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Citations for anatrace :
251 - 286 of 286 citations for N N Dimethyl 4 4 Trimethylsilyl Ethynyl Phenyl Ethynyl Aniline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... SLC45A4 was purified to homogeneity using standard immobilised affinity chromatography (IMAC) protocols in n-either dodecyl-β-d-maltopyranoside (DDM) detergent (Anatrace) with cholesterol hemisuccinate (5:1 ratio DDM ...
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bioRxiv - Plant Biology 2024Quote: ... thylakoid membranes (400–800 µg Chl at 0.5 mg Chl/mL) were solubilized with 1% (w/v) n-dodecyl-α-D-maltoside (α-DDM) (Anatrace) for 30 min with gentle agitation ...
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bioRxiv - Biophysics 2024Quote: ... mechanically homogenized and solubilized in the solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-dodecyl-β-D-maltopyranoside (DDM; Anatrace, D310) and 0.2% cholesteryl hemisuccinate Tris salt (Anatrace ...
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bioRxiv - Biochemistry 2024Quote: ... were sonicated for total of 6-10 minutes and then solubilized using 8% (wt/vol) of n-octyl-β-D-maltoside (OM; Anatrace). Purified chicken BEST1 and SEC buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were solubilized by addition of an equal volume of ACA 750 containing 2% n-dodecyl β-D-maltoside (β-DM, Anatrace) for a final concentration of 0.5 mg mL−1 chlorophyll and 1% β-DM ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 mM BME and further solubilized by stirring with the addition of 1% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, OH) for an hour at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... proteins were overexpressed in BL21-AI E.coli competent cells and extracted into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris(2-carboxyethyl)phosphine ...
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bioRxiv - Biochemistry 2022Quote: ... or either 2 mM lyso PC/PG (50:50 mol% of 14:0 lyso PC and 14:0 lyso PG) or 1 mM β-DDM (n-Dodecyl β-D-maltoside) (Anatrace) were added to the samples ...
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bioRxiv - Biophysics 2022Quote: ... The membrane was resuspended again using Buffer W and solubilized by the addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM) (Anatrace, USA), 0.15% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 30 minutes rocking at room temperature after addition of DNase, lysozyme, protease cocktail inhibitor (Pierce), and n-octyl-β-glucopyranoside detergent (β-OG, 2% final concentration) (Anatrace). Lysate was clarified by centrifugation (20 min ...
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bioRxiv - Neuroscience 2024Quote: ... Membrane pellets were scooped into a Dounce homogenizer containing extraction buffer (50 mM TRIS, 150 mM KCl, 1 mM EDTA, 1.5% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.3% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was discarded and membrane pellets were scooped into a dounce homogenizer containing extraction buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), pH 8) ...
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bioRxiv - Biophysics 2022Quote: ... Membrane pellets were scooped into a dounce homogenizer containing extraction buffer (50 mM HEPES, 150 mM KCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Biophysics 2021Quote: ... Membrane pellets were scooped into a dounce homogenizer containing Extraction Buffer (50 mM Tris, 150 mM KCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), pH 8) ...
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bioRxiv - Biophysics 2022Quote: ... the initial purification for nanodisc incorporation was performed analogously to the GDN prep with the following modifications: Extraction buffer contained 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% Cholesterol Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Biophysics 2023Quote: ... coli cells transformed with the expression plasmid were lysed by sonication and the protein extracted from membrane fragments into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris (2-carboxyethyl ...
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bioRxiv - Biochemistry 2020Quote: ... was added before cruel-extraction at 4°C in 1.5% DDM and 0.3% CHS (Anatrace) for 2 hours ...
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bioRxiv - Biophysics 2024Quote: ... Membranes were solubilised by constant stirring for 1.5 hours at 4°C in DDM (Anatrace) at a final 1% w/v concentration ...
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bioRxiv - Neuroscience 2020Quote: ... The plasma membranes were solubilized at 4 °C with 10x critical micelle concentration (CMC) DDM (Anatrace) in a solution of 20mM HEPES (pH8.0) ...
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bioRxiv - Biophysics 2022Quote: DDM-solubilized claudin-4 was exchanged into 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside (LMNG, Anatrace) or amphipol A8-35 (Anatrace) ...
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bioRxiv - Biophysics 2023Quote: Cells were solubilized for 1 hour at 4 °C in 1% (w/v) Glyco-diosgenin (GDN) (Anatrace). Insoluble material was removed by centrifugation at 16,000 rpm for 30 minutes at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... DDM solubilized hsCLDN-4 was washed with buffer containing 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside (LMNG, Anatrace) and released by thrombin ...
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bioRxiv - Biochemistry 2021Quote: ... Nanodiscs were assembled on the resin by adding 1.25mM lipids (POPC/DOPE (Avanti) at a 4:1 ratio in DDM (Anatrace)) and 25uM nanodisc-scaffolding protein MSP1D1 in 700ul of washing buffer ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... or concentrated to 4 mg/mL and vitrified in the presence of 1.5 mM fluorinated fos-choline (FFosC, Anatrace). 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT and incubated 1h at 4 °C with PMAL-C8 (Anatrace, mass ratio protein:PMAL-C8 1:2.25). 30 mg BioBeads (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... Then the membrane was solubilized for 3 h at 4 °C by adding 0.5% (w/v) lauryl maltoseneopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Solubilization of 8S complexes was performed by mixing the homogenized sample at 4°C for 2 hours after addition of 2% C12M (Anatrace). After removing the insoluble material by centrifugation at 42,000 rpm (Ti-45 rotor ...
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bioRxiv - Biochemistry 2022Quote: ... UBR5 was frozen in GF Buffer with the addition of 4 mM CHAPSO or 0.005 % fluorinated octyl β-maltoside (Anatrace) prior to freezing.
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: Native and selenomethionine-labeled LapD2 was concentrated to 10 mg/ml and screened for crystallization conditions using the MCSG1-4 crystallization suites (Anatrace) and the hanging drop vapour diffusion method ...
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bioRxiv - Biochemistry 2022Quote: ... diluted with 4 mL GTBS (50 mM Tris-HCl, 150 mM NaCl, 0.004% [w/v] glyco-diosgenin [GDN; Anatrace], pH 7.4) and concentrated to ~200 μL in the same concentrator ...
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bioRxiv - Biochemistry 2023Quote: ... the receptor was solubilized for 2 hrs at 4°C with continual stirring in a solution of 0.5% L-MNG (Anatrace, Cat. no: NG310) and 0.1% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals ...
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bioRxiv - Biochemistry 2022Quote: ... Drops of 450 nL of 10 mg/mL protein in SEC buffer and 150 nL of MCSG-1 H10 (Anatrace; 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...