Labshake search
Citations for anatrace :
251 - 298 of 298 citations for N Ethyl Benzamid 3 carboxylate 15N 13C2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Two membrane pellets (corresponding to half a fermenter growth) were thawed and solubilized with addition of n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) to 1% (w/v ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 200 μg/ml purified proteins were added into liposomes after incubation with 1% n-octyl-ß-D-glucopyranoside (ß-OG, Anatrace) at 4 °C for 30 min ...
-
bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
-
bioRxiv - Biophysics 2022Quote: ... for 30 min at 4 °C and then solubilized by addition of 2× buffer (50 mM HEPES, 500 mM NaCl, 2 %w/v n-dodecyl-β-D-maltopyranoside (DDM; Anatrace), 0.4 %w/v cholesteryl hemisuccinate (CHS ...
-
bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
-
bioRxiv - Microbiology 2022Quote: ... and extracted with detergent by adding drop-wise a 10% (w/v) stock solution of n-dodecyl β-D-maltoside (DDM, Anatrace) to a final concentration of 1.3% (w/v) ...
-
bioRxiv - Immunology 2020Quote: ... RBD or S1-binding clones were moved into the ReD cell-display platform (Beasley et al. 2015) and cells were membrane permeabilized using 0.5% n-octyl b-d-thioglucopyranoside (Anatrace, Cat: 0314) and labeled for FACS using either SARS-CoV-2 RBD-Fc (Sino Biological ...
-
bioRxiv - Molecular Biology 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [HN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
-
bioRxiv - Biophysics 2023Quote: ... washed once with PBS and were resuspended with 500 μL buffer A containing 1% N-Dodecyl-β-D-maltoside (DDM, Anatrace), 0.1% cholesteryl hemisuccinate (CHS ...
-
bioRxiv - Biochemistry 2023Quote: ... The mixture was then incubated at room temperature (RT) for 1 hour and the membranes were solubilised by adding 1% (w/v) n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace), and 0.2% (w/v ...
-
bioRxiv - Biophysics 2023Quote: ... which were solubilized in solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-Dodecyl-β-D-Maltopyranoside (Anatrace, D310) and 0.2% Cholesteryl Hemisuccinate Tris salt (Anatrace ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The mixture was transferred to 4 °C and allowed to equilibrate for 20 minutes before solubilization was initiated by addition of a final concentration of 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), 0.2% (w/v ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were lysed with the Microfluidizer LM20 (Microfluidics) cell disruptor and lysate was incubated with 1% N-Dodecyl-β-D-maltoside (Anatrace) for 1 1hour at 4°C with rocking ...
-
bioRxiv - Plant Biology 2022Quote: ... Then the suspension was incubated in the buffer with additional l.5 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) at 4 °C for 2 h ...
-
bioRxiv - Biochemistry 2022Quote: HEK293F cells (for SBA clarified lysate preparation) and HEK293T cells (for expression analysis by Western Blot) were solubilized with n-Dodecyl-β-d-maltoside (DM) detergent (Anatrace) to form micelles around membrane proteins and maintain GPCR and RAMP structure and complex formation ...
-
bioRxiv - Biophysics 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [TN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
-
bioRxiv - Biochemistry 2023Quote: Frozen membranes were thawed in a tepid water bath and solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM; Anatrace) with mixing for ∼1 h ...
-
bioRxiv - Microbiology 2024Quote: ... and pellets were dissolved in solubilization buffer (20 mM Tris, 150 NaCl, 15% glycerol, 2% (w/v) n-dodecyl-β-D-maltoside (DDM; Anatrace) and 0.2% (w/v ...
-
bioRxiv - Neuroscience 2024Quote: ... SLC45A4 was purified to homogeneity using standard immobilised affinity chromatography (IMAC) protocols in n-either dodecyl-β-d-maltopyranoside (DDM) detergent (Anatrace) with cholesterol hemisuccinate (5:1 ratio DDM ...
-
bioRxiv - Plant Biology 2024Quote: ... thylakoid membranes (400–800 µg Chl at 0.5 mg Chl/mL) were solubilized with 1% (w/v) n-dodecyl-α-D-maltoside (α-DDM) (Anatrace) for 30 min with gentle agitation ...
-
bioRxiv - Biochemistry 2024Quote: ... were sonicated for total of 6-10 minutes and then solubilized using 8% (wt/vol) of n-octyl-β-D-maltoside (OM; Anatrace). Purified chicken BEST1 and SEC buffer ...
-
bioRxiv - Biophysics 2024Quote: ... mechanically homogenized and solubilized in the solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-dodecyl-β-D-maltopyranoside (DDM; Anatrace, D310) and 0.2% cholesteryl hemisuccinate Tris salt (Anatrace ...
-
bioRxiv - Plant Biology 2022Quote: ... Membranes were solubilized by addition of an equal volume of ACA 750 containing 2% n-dodecyl β-D-maltoside (β-DM, Anatrace) for a final concentration of 0.5 mg mL−1 chlorophyll and 1% β-DM ...
-
bioRxiv - Biochemistry 2021Quote: ... and 5 mM BME and further solubilized by stirring with the addition of 1% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, OH) for an hour at 4 °C ...
-
bioRxiv - Biophysics 2021Quote: ... proteins were overexpressed in BL21-AI E.coli competent cells and extracted into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris(2-carboxyethyl)phosphine ...
-
bioRxiv - Biochemistry 2022Quote: ... or either 2 mM lyso PC/PG (50:50 mol% of 14:0 lyso PC and 14:0 lyso PG) or 1 mM β-DDM (n-Dodecyl β-D-maltoside) (Anatrace) were added to the samples ...
-
bioRxiv - Biophysics 2022Quote: ... The membrane was resuspended again using Buffer W and solubilized by the addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM) (Anatrace, USA), 0.15% (w/v ...
-
bioRxiv - Neuroscience 2024Quote: ... Membrane pellets were scooped into a Dounce homogenizer containing extraction buffer (50 mM TRIS, 150 mM KCl, 1 mM EDTA, 1.5% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.3% cholesteryl hemisuccinate Tris salt (CHS ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by 30 minutes rocking at room temperature after addition of DNase, lysozyme, protease cocktail inhibitor (Pierce), and n-octyl-β-glucopyranoside detergent (β-OG, 2% final concentration) (Anatrace). Lysate was clarified by centrifugation (20 min ...
-
bioRxiv - Cell Biology 2024Quote: ... and blotted again with 4 μL of a solution containing lysis buffer supplemented with 0.1 %(v/v) n-octyl-β-D-glucopyranoside (β-OG, Anatrace #O311). The grid was then transferred to an EM GP2 plunger (Leica ...
-
bioRxiv - Biochemistry 2020Quote: Purified samples were supplemented with 3 mM fluorinated Fos-Choline-8 (FFC8; Anatrace) before plunge freezing ...
-
bioRxiv - Neuroscience 2021Quote: ... The supernatant was discarded and membrane pellets were scooped into a dounce homogenizer containing extraction buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), pH 8) ...
-
bioRxiv - Biophysics 2022Quote: ... Membrane pellets were scooped into a dounce homogenizer containing extraction buffer (50 mM HEPES, 150 mM KCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% cholesteryl hemisuccinate Tris salt (CHS ...
-
bioRxiv - Plant Biology 2020Quote: ... at 2.0 mg Chl/mL and solubilized with 4% (w/v) n-dodecyl-α-D-maltoside (α-DDM as previously abbreviated as α-DM; Anatrace) for 30 min with gentle agitation on ice ...
-
bioRxiv - Biophysics 2021Quote: ... Membrane pellets were scooped into a dounce homogenizer containing Extraction Buffer (50 mM Tris, 150 mM KCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), pH 8) ...
-
bioRxiv - Biophysics 2022Quote: ... the initial purification for nanodisc incorporation was performed analogously to the GDN prep with the following modifications: Extraction buffer contained 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% Cholesterol Hemisuccinate Tris Salt (CHS ...
-
bioRxiv - Biophysics 2023Quote: ... coli cells transformed with the expression plasmid were lysed by sonication and the protein extracted from membrane fragments into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris (2-carboxyethyl ...
-
bioRxiv - Biochemistry 2022Quote: 2.5mg of of a POPC/POPG 3/1 (w/w) mixture in chloroform (Anatrace) were dried for 3h under argon flow ...
-
bioRxiv - Biochemistry 2022Quote: 2.5mg of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) in chloroform (Anatrace) were dried for 3h under argon flow ...
-
bioRxiv - Microbiology 2024Quote: ... 3 μL of each complex was briefly incubated with lauryl maltose neopentyl glycol (LMNG; Anatrace) to a final concentration of 0.005 mM and then deposited on glow discharged Quantifoil Cu 1.2/1.3-300 mesh grids and plunge frozen using a Thermo Fisher Vitrobot Mark IV at 4ᵒC ...
-
bioRxiv - Immunology 2020Quote: ... 3 μL of complex was mixed with 0.5 µL of a 0.5% w/v octyl-maltoside solution (Anatrace). Samples were vitrified in 100% liquid ethane using a Mark IV Vitrobot (Thermo Fisher ...
-
bioRxiv - Biophysics 2024Quote: ... Cells were lysed in RIPA buffer (150 mM NaCl, 3 mM MgCl2, 1% NP-40, 0.5% deoxycholate (Anatrace), 0.1% SDS ...
-
bioRxiv - Biophysics 2024Quote: ... Cells were lysed in RIPA buffer (150 mM NaCl, 3 mM MgCl2, 1% NP-40, 0.5% deoxycholate (Anatrace), 0.1% SDS ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were collected and centrifuged at 500 x g for 3 minutes, resuspended with lysis buffer (150 mM NaCl, 50 mM HEPES, pH 7.5, 1% (w/v) DDM (Anatrace), 1 mM PMSF) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
-
Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... Then the membrane was solubilized for 3 h at 4 °C by adding 0.5% (w/v) lauryl maltoseneopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
-
bioRxiv - Biophysics 2020Quote: ... Concentrated PilQ (150 µL of ∼1 mg/mL protein) was exchanged into Amphipol A8-35 (0.585 mg for a 3:1 ratio, Anatrace, A835) and allowed to incubate at 4 C for 1 hr ...
-
bioRxiv - Biophysics 2023Quote: ... Cell powder was added at a ratio of 1 g cell powder to 3 ml lysis buffer (200 mM KCl, 21 mM OGNG (octyl glucose neopentyl glycol, Anatrace), 30 mM HTG (n-heptyl-β-D-thioglucopyranoside ...