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Citations for anatrace :
251 - 277 of 277 citations for D Galactose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... which were solubilized in solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-Dodecyl-β-D-Maltopyranoside (Anatrace, D310) and 0.2% Cholesteryl Hemisuccinate Tris salt (Anatrace ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The mixture was transferred to 4 °C and allowed to equilibrate for 20 minutes before solubilization was initiated by addition of a final concentration of 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), 0.2% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed with the Microfluidizer LM20 (Microfluidics) cell disruptor and lysate was incubated with 1% N-Dodecyl-β-D-maltoside (Anatrace) for 1 1hour at 4°C with rocking ...
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bioRxiv - Biophysics 2022Quote: ... The membrane pellets were homogenized in lysis buffer supplemented with 200 mM NaCl and 10% (v/v) glycerol (solubilization buffer) and then incubated with 1% dodecyl-β-D-maltopyranoside (DDM; Anatrace) 0.2% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Plant Biology 2022Quote: ... Then the suspension was incubated in the buffer with additional l.5 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) at 4 °C for 2 h ...
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bioRxiv - Plant Biology 2023Quote: ... the thylakoid membranes were diluted to a concentration of 0.5 mg chlorophyll mL-1 and solubilized with 1% (w/v) dodecyl-β-D-maltoside (β-DDM) (Anatrace) for 30 min on ice ...
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bioRxiv - Biochemistry 2022Quote: HEK293F cells (for SBA clarified lysate preparation) and HEK293T cells (for expression analysis by Western Blot) were solubilized with n-Dodecyl-β-d-maltoside (DM) detergent (Anatrace) to form micelles around membrane proteins and maintain GPCR and RAMP structure and complex formation ...
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bioRxiv - Biophysics 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [TN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biochemistry 2023Quote: Frozen membranes were thawed in a tepid water bath and solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM; Anatrace) with mixing for ∼1 h ...
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bioRxiv - Microbiology 2024Quote: ... and pellets were dissolved in solubilization buffer (20 mM Tris, 150 NaCl, 15% glycerol, 2% (w/v) n-dodecyl-β-D-maltoside (DDM; Anatrace) and 0.2% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... SLC45A4 was purified to homogeneity using standard immobilised affinity chromatography (IMAC) protocols in n-either dodecyl-β-d-maltopyranoside (DDM) detergent (Anatrace) with cholesterol hemisuccinate (5:1 ratio DDM ...
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bioRxiv - Plant Biology 2024Quote: ... thylakoid membranes (400–800 µg Chl at 0.5 mg Chl/mL) were solubilized with 1% (w/v) n-dodecyl-α-D-maltoside (α-DDM) (Anatrace) for 30 min with gentle agitation ...
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bioRxiv - Biochemistry 2024Quote: ... were sonicated for total of 6-10 minutes and then solubilized using 8% (wt/vol) of n-octyl-β-D-maltoside (OM; Anatrace). Purified chicken BEST1 and SEC buffer ...
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bioRxiv - Biophysics 2024Quote: ... mechanically homogenized and solubilized in the solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-dodecyl-β-D-maltopyranoside (DDM; Anatrace, D310) and 0.2% cholesteryl hemisuccinate Tris salt (Anatrace ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were solubilized by addition of an equal volume of ACA 750 containing 2% n-dodecyl β-D-maltoside (β-DM, Anatrace) for a final concentration of 0.5 mg mL−1 chlorophyll and 1% β-DM ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 mM BME and further solubilized by stirring with the addition of 1% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, OH) for an hour at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... proteins were overexpressed in BL21-AI E.coli competent cells and extracted into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris(2-carboxyethyl)phosphine ...
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bioRxiv - Biochemistry 2022Quote: ... or either 2 mM lyso PC/PG (50:50 mol% of 14:0 lyso PC and 14:0 lyso PG) or 1 mM β-DDM (n-Dodecyl β-D-maltoside) (Anatrace) were added to the samples ...
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bioRxiv - Biophysics 2022Quote: ... The membrane was resuspended again using Buffer W and solubilized by the addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM) (Anatrace, USA), 0.15% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... Membrane pellets were scooped into a Dounce homogenizer containing extraction buffer (50 mM TRIS, 150 mM KCl, 1 mM EDTA, 1.5% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.3% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Cell Biology 2024Quote: ... and blotted again with 4 μL of a solution containing lysis buffer supplemented with 0.1 %(v/v) n-octyl-β-D-glucopyranoside (β-OG, Anatrace #O311). The grid was then transferred to an EM GP2 plunger (Leica ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was discarded and membrane pellets were scooped into a dounce homogenizer containing extraction buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), pH 8) ...
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bioRxiv - Biophysics 2022Quote: ... Membrane pellets were scooped into a dounce homogenizer containing extraction buffer (50 mM HEPES, 150 mM KCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Plant Biology 2020Quote: ... at 2.0 mg Chl/mL and solubilized with 4% (w/v) n-dodecyl-α-D-maltoside (α-DDM as previously abbreviated as α-DM; Anatrace) for 30 min with gentle agitation on ice ...
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bioRxiv - Biophysics 2021Quote: ... Membrane pellets were scooped into a dounce homogenizer containing Extraction Buffer (50 mM Tris, 150 mM KCl, 1 mM EDTA, 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), pH 8) ...
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bioRxiv - Biophysics 2022Quote: ... the initial purification for nanodisc incorporation was performed analogously to the GDN prep with the following modifications: Extraction buffer contained 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% Cholesterol Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Biophysics 2023Quote: ... coli cells transformed with the expression plasmid were lysed by sonication and the protein extracted from membrane fragments into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris (2-carboxyethyl ...