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Citations for anatrace :
251 - 289 of 289 citations for Cow Polypyrimidine tract binding protein 1 PTBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... then solubilised on ice at 4.5 mg mL−1 by the stepwise addition of lauryl maltose neopentyl glycol (LMNG, Anatrace) from a 5% stock solution to a final concentration of 1.5% ...
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bioRxiv - Biochemistry 2024Quote: 3.5 uL of nhTMEM16-containing nanodiscs (3-7mg ml-1) supplemented with 1.5 or 3 mM Fos-Choline-8-Fluorinated (Anatrace) was applied to a glow-discharged UltrAuFoil R1.2/1.3 300-mesh gold grid (Quantifoil ...
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bioRxiv - Plant Biology 2020Quote: ... and were suspended in a 25 mM MES buffer (pH 6.5) containing 1 M betaine at 0.3 mg Chl/mL and solubilized with 1.0% α-DDM (Anatrace, Maumee, OH) for 10 min on ice ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Biophysics 2021Quote: ... with 1 % (v/v) membrane protease inhibitors and 1.0 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM) 40:1 (w/w) CHS (Anatrace). After centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) using a Dounce homogenizer and this membrane suspension was solubilized with 1% dodecyl-maltoside (DDM, Anatrace) and 0.1% cholesteryl hemi-succinate (CHS ...
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bioRxiv - Biophysics 2022Quote: ... The pellet containing the membrane fraction was solubilized in 1% N-dodecyl-β-D-maltopyranoside (DDM) and 0.1% cholesteryl hemisuccinate (CHS; Tris Salt, Anatrace) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... the detergent was exchanged with 16 CVs of wash buffer I supplemented with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... equilibrated with the FPLC buffer (20 mM HEPES, pH7.5, 150 mM NaCl) plus 1 mM (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace).
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed in ATF6 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 1x protease inhibitor, and 1% LMNG (Anatrace NG322) for 15 min at 4°C as previously described68 ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 mM MgCl2 were added to the thawed suspension and the mixture was incubated with additional 1% (w/v) dodecyl-β-D-maltopyranoside (DDM; Bluepus) and 0.2% (w/v) cholesteryl hemisuccinate (CHS, Anatrace) at 8°C for 2 h for membrane solubilization and protein extraction ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were solubilized by resuspending in buffer (50 mM Tris-HCl, pH 7.4, 10% [v/v] glycerol, 1% [w/w] dodecyl-β-D-maltoside [DDM, Anatrace] ...
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bioRxiv - Plant Biology 2023Quote: ... Tissue was lysed in 500 μl of lysis buffer (50 mM Tris-HCl pH 7.2, 10% v/v glycerol, 150 mM NaCl, 1% w/v DIBMA (Anatrace BMA101), with protease inhibitors (1% v/v ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were mechanically broken and were resuspended in buffer A (10ml of buffer per gram of membrane) and supplemented with 1% w/v final of dodecyl-maltoside (DDM, Anatrace) for 1h solubilization under gentle stirring ...
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bioRxiv - Immunology 2023Quote: ... The membrane pellet was thoroughly resuspended in TN buffer using a homogenizer and then mixed with a pre-made solution of 10% (w/v) Dodecyl Maltoside (DDM) and 1% (w/v) Cholesteryl Hemisuccinate (CHS) (Anatrace) to a final concentration of 1% DDM and 0.1% CHS ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were solubilized by incubating with an addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction was homogenized on ice with Buffer B (20 mL/pellet) containing 1% dodecylphosphocholine (DPC, FOS-CHOLINE-12, Anatrace). Homogenized cells were ultracentrifuged at 100,000 x g for 30 minutes to remove the insoluble fraction ...
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bioRxiv - Biochemistry 2023Quote: ... for rounds 2-5 phage particles were eluted from magnetic beads by a 15-min incubation step with 1% Fos-choline-12 (Anatrace) prepared in buffered saline.
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bioRxiv - Biochemistry 2023Quote: ... 1,2-Dimyristoyl-sn-glycerol-3-phosphocholine (DMPC) and 3((3-cholamidopropyl)- dimethylammonio)-2-hydroxy-1-propanesulphonate (CHAPSO) were purchased from Anatrace. Sodium dodecyl sulfate (SDS ...
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bioRxiv - Biochemistry 2023Quote: ... and receptor was subsequently extracted from membranes in a volume of 50 ml by addition of 2× solubilisation buffer (100 mM HEPES pH 7.5, 250 mM NaCl, 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... Cell powder was added at a ratio of 1 g cell powder to 3 ml lysis buffer (200 mM KCl, 21 mM OGNG (octyl glucose neopentyl glycol, Anatrace), 30 mM HTG (n-heptyl-β-D-thioglucopyranoside ...
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bioRxiv - Biochemistry 2020Quote: AcrB was extracted from homogenized membranes by overnight incubation with 1 % (w/v) n-dodecyl-β-D-maltoside (DDM) detergent (Anatrace) at 4 °C with gentle agitation ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
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bioRxiv - Biophysics 2022Quote: ... The membrane pellets were homogenized in lysis buffer supplemented with 200 mM NaCl and 10% (v/v) glycerol (solubilization buffer) and then incubated with 1% dodecyl-β-D-maltopyranoside (DDM; Anatrace) 0.2% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Molecular Biology 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [HN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [TN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biochemistry 2023Quote: Frozen membranes were thawed in a tepid water bath and solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM; Anatrace) with mixing for ∼1 h ...
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bioRxiv - Biophysics 2023Quote: ... washed once with PBS and were resuspended with 500 μL buffer A containing 1% N-Dodecyl-β-D-maltoside (DDM, Anatrace), 0.1% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biophysics 2023Quote: ... which were solubilized in solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-Dodecyl-β-D-Maltopyranoside (Anatrace, D310) and 0.2% Cholesteryl Hemisuccinate Tris salt (Anatrace ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The mixture was transferred to 4 °C and allowed to equilibrate for 20 minutes before solubilization was initiated by addition of a final concentration of 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), 0.2% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed with the Microfluidizer LM20 (Microfluidics) cell disruptor and lysate was incubated with 1% N-Dodecyl-β-D-maltoside (Anatrace) for 1 1hour at 4°C with rocking ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 mM BME and further solubilized by stirring with the addition of 1% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, OH) for an hour at 4 °C ...
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bioRxiv - Biochemistry 2019Quote: ... Cholesteryl hemisuccinate Tris salt (CHS) and detergents 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) and n-dodecyl-β-D-maltoside (DDM) were from Anatrace (anatrace.com).
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bioRxiv - Biochemistry 2022Quote: ... or either 2 mM lyso PC/PG (50:50 mol% of 14:0 lyso PC and 14:0 lyso PG) or 1 mM β-DDM (n-Dodecyl β-D-maltoside) (Anatrace) were added to the samples ...
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bioRxiv - Biophysics 2022Quote: ... The membrane was resuspended again using Buffer W and solubilized by the addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM) (Anatrace, USA), 0.15% (w/v ...
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bioRxiv - Biophysics 2022Quote: ... the initial purification for nanodisc incorporation was performed analogously to the GDN prep with the following modifications: Extraction buffer contained 1% n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace, Maumee, OH), 0.2% Cholesterol Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Biochemistry 2023Quote: ... The cell pellet was re-dissolved and dounced in solubilization buffer (50 mM Tris-Cl, pH 8.0, 150 mM NaCl, 5 mM β-mercaptoethanol, 1% DDM (Anatrace, Cat. no. D310), 1 mM PMSF ...