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Citations for anatrace :
201 - 250 of 265 citations for Oxytocin ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... AcrAL was solubilised from homogenised membranes with 1% (w/v) n-dodecyl-β-D-maltoside (DDM) detergent (Anatrace) at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... of buffer A containing 0.1% DDM and 0.01% CHS before being incubated with 1% lauryl maltose neopentyl glycol (LMNG, Anatrace) and 0.1% CHS in buffer A at 4 °C for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane proteins were extracted by supplementing the lysis buffer with 1% n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), and incubated for 1.5 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate was subsequently incubated for 1 h at room temperature (RT) and then solubilized by 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace) and 0.1% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... and resuspended in 500 μl solubilization buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 1% (w/v) DDM (Anatrace), protease inhibitor cocktail set III (Merck Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were collected and centrifuged at 500 x g for 3 minutes, resuspended with lysis buffer (150 mM NaCl, 50 mM HEPES, pH 7.5, 1% (w/v) DDM (Anatrace), 1 mM PMSF) ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysate was dounce homogenized (8 strokes with the A pestle) and a 1% Dodecyl maltoside (DDM)/0.2% Cholesteryl hemisuccinate (CHS) (Anatrace) w:w mixture was added for protein solubilization ...
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bioRxiv - Biochemistry 2021Quote: ... pre-equilibrated with size exclusion buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 1 mM DDM (Anatrace)) in line with a fluorescence detector (RF-20A ...
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bioRxiv - Biochemistry 2021Quote: ... Nanodiscs were assembled on the resin by adding 1.25mM lipids (POPC/DOPE (Avanti) at a 4:1 ratio in DDM (Anatrace)) and 25uM nanodisc-scaffolding protein MSP1D1 in 700ul of washing buffer ...
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bioRxiv - Biophysics 2022Quote: ... before being resuspended in buffer (50 mM Tris-HCl pH 7.4, 10% [v/v] glycerol, 1% [w/w] DDM [Anatrace] ...
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bioRxiv - Biophysics 2022Quote: ... membrane pellets were homogenized in TBS (20 mM Tris-HCl, pH 7.5, 150 mM NaCl) and solubilized in TBS with 1% GDN (Anatrace), supplemented with protease inhibitors (1 mg/ml Leupeptin ...
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bioRxiv - Biochemistry 2022Quote: ... Heterotrimeric Giβ1γ2 was extracted in a buffer containing 1% sodium cholate and 0.05% n-dodecyl-β-D-maltoside (DDM, Anatrace). Ni- NTA chromatography is performed and the detergent was exchanged from cholate/DDM to DDM on column ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the GLUT3 protein was purified in 0.06% (w/v) 6-cyclohexyl-1-hexyl-ß-D-maltoside (Cymal-6, Anatrace) and concentrated to ~ 40 mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ROS of 50 bovine retinas were isolated as described previously and suspended in Extraction Buffer (20 mM BTP, pH 7.9, 10% glycerol, 300 mM NaCl, 1 mM TCEP) containing 2% LMNG (Anatrace) (Papermaster ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were resuspended in TBS and solubilized by incubation with 1% (w/v) lauryl maltose neopentyl glycol (LMNG; Anatrace) for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... The size exclusion purified GspLM was concentrated and mixed with 10:1 molar excess of Amphipol A8-35 (Anatrace), for 30 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was incubated for 1 h at room temperature and complex from membranes solubilized by 0.5% (w/v) lauryl maltose neopentylglycol (LMNG, Anatrace) supplemented with 0.1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... Membranes were resuspended by manual homogenization in a dounce in lysis buffer supplemented with 1% glycol-diosgenin (GDN) (Anatrace) and incubated for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was incubated for 1 h at room temperature and then solubilized by 0.5% (w/v) lauryl maltose neopentylglycol (LMNG, Anatrace) supplemented with 0.1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction was pelleted at 100.000 × g and solvated in 1 % (w/v) Dodecyl-malto-pyranoside (DDM, Anatrace). Affinity chromatography with Ni-NTA-Agarose (Peqlab ...
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bioRxiv - Biophysics 2023Quote: ... After resuspension in buffer B (buffer A supplemented with 1% (w/v) n-Dodecyl-β-D-maltoside (DDM, Anatrace), 0.15% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... then solubilised on ice at 4.5 mg mL−1 by the stepwise addition of lauryl maltose neopentyl glycol (LMNG, Anatrace) from a 5% stock solution to a final concentration of 1.5% ...
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bioRxiv - Biochemistry 2024Quote: 3.5 uL of nhTMEM16-containing nanodiscs (3-7mg ml-1) supplemented with 1.5 or 3 mM Fos-Choline-8-Fluorinated (Anatrace) was applied to a glow-discharged UltrAuFoil R1.2/1.3 300-mesh gold grid (Quantifoil ...
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bioRxiv - Plant Biology 2020Quote: ... and were suspended in a 25 mM MES buffer (pH 6.5) containing 1 M betaine at 0.3 mg Chl/mL and solubilized with 1.0% α-DDM (Anatrace, Maumee, OH) for 10 min on ice ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Biophysics 2021Quote: ... with 1 % (v/v) membrane protease inhibitors and 1.0 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM) 40:1 (w/w) CHS (Anatrace). After centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) using a Dounce homogenizer and this membrane suspension was solubilized with 1% dodecyl-maltoside (DDM, Anatrace) and 0.1% cholesteryl hemi-succinate (CHS ...
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bioRxiv - Biophysics 2022Quote: ... The pellet containing the membrane fraction was solubilized in 1% N-dodecyl-β-D-maltopyranoside (DDM) and 0.1% cholesteryl hemisuccinate (CHS; Tris Salt, Anatrace) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... the detergent was exchanged with 16 CVs of wash buffer I supplemented with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... equilibrated with the FPLC buffer (20 mM HEPES, pH7.5, 150 mM NaCl) plus 1 mM (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace).
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed in ATF6 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 1x protease inhibitor, and 1% LMNG (Anatrace NG322) for 15 min at 4°C as previously described68 ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 mM MgCl2 were added to the thawed suspension and the mixture was incubated with additional 1% (w/v) dodecyl-β-D-maltopyranoside (DDM; Bluepus) and 0.2% (w/v) cholesteryl hemisuccinate (CHS, Anatrace) at 8°C for 2 h for membrane solubilization and protein extraction ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were solubilized by resuspending in buffer (50 mM Tris-HCl, pH 7.4, 10% [v/v] glycerol, 1% [w/w] dodecyl-β-D-maltoside [DDM, Anatrace] ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were mechanically broken and were resuspended in buffer A (10ml of buffer per gram of membrane) and supplemented with 1% w/v final of dodecyl-maltoside (DDM, Anatrace) for 1h solubilization under gentle stirring ...
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bioRxiv - Plant Biology 2023Quote: ... Tissue was lysed in 500 μl of lysis buffer (50 mM Tris-HCl pH 7.2, 10% v/v glycerol, 150 mM NaCl, 1% w/v DIBMA (Anatrace BMA101), with protease inhibitors (1% v/v ...
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bioRxiv - Biochemistry 2023Quote: ... and receptor was subsequently extracted from membranes in a volume of 50 ml by addition of 2× solubilisation buffer (100 mM HEPES pH 7.5, 250 mM NaCl, 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were solubilized by incubating with an addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... membranes at a protein concentration of ∼4 mg/ml were solubilized with 1% (w:v) n-dodecyl-β-d-maltopyranoside (DDM) (Anatrace) and 0.1% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Immunology 2023Quote: ... The membrane pellet was thoroughly resuspended in TN buffer using a homogenizer and then mixed with a pre-made solution of 10% (w/v) Dodecyl Maltoside (DDM) and 1% (w/v) Cholesteryl Hemisuccinate (CHS) (Anatrace) to a final concentration of 1% DDM and 0.1% CHS ...
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bioRxiv - Biochemistry 2023Quote: ... 1,2-Dimyristoyl-sn-glycerol-3-phosphocholine (DMPC) and 3((3-cholamidopropyl)- dimethylammonio)-2-hydroxy-1-propanesulphonate (CHAPSO) were purchased from Anatrace. Sodium dodecyl sulfate (SDS ...
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bioRxiv - Biochemistry 2023Quote: ... for rounds 2-5 phage particles were eluted from magnetic beads by a 15-min incubation step with 1% Fos-choline-12 (Anatrace) prepared in buffered saline.
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction was homogenized on ice with Buffer B (20 mL/pellet) containing 1% dodecylphosphocholine (DPC, FOS-CHOLINE-12, Anatrace). Homogenized cells were ultracentrifuged at 100,000 x g for 30 minutes to remove the insoluble fraction ...
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bioRxiv - Biophysics 2023Quote: ... Cell powder was added at a ratio of 1 g cell powder to 3 ml lysis buffer (200 mM KCl, 21 mM OGNG (octyl glucose neopentyl glycol, Anatrace), 30 mM HTG (n-heptyl-β-D-thioglucopyranoside ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Giβ1ψ2 G-protein were lysed in hypotonic buffer and G-protein was extracted in a buffer containing 1% sodium cholate and 0.05% n-dodecyl-β-D-maltoside (DDM, Anatrace). Detergent was exchanged from cholate/DDM to DDM on Ni Sepharose column ...
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bioRxiv - Biophysics 2022Quote: ... and the membrane pellet was resuspended in Lysis buffer and the protein was solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.04% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biochemistry 2020Quote: AcrB was extracted from homogenized membranes by overnight incubation with 1 % (w/v) n-dodecyl-β-D-maltoside (DDM) detergent (Anatrace) at 4 °C with gentle agitation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 200 μg/ml purified proteins were added into liposomes after incubation with 1% n-octyl-ß-D-glucopyranoside (ß-OG, Anatrace) at 4 °C for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...