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Citations for anatrace :
201 - 250 of 267 citations for N Acetyl methyl amino dimethylsilyl N methylacetamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The resulting pellets were resuspended in Solubilization Buffer (PBS, 1% n-dodecyl-β-maltoside (DDM, Anatrace, catalog number D310), 37 mM imidazole ...
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bioRxiv - Plant Biology 2024Quote: ... The thylakoid membranes were solubilized with 1% (w/v) N-dodecyl-α-D-maltoside (α- DDM) (Anatrace, Maumee, OH) for 30 min on ice and centrifuged at 40,000g for 10 min to remove the unsolubilized part ...
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bioRxiv - Biochemistry 2024Quote: The resulting pellet was resuspended in 10 mL of lysis buffer per 1 g of cell mass (25 mM HEPES, pH 7.5, 150 mM NaCl, 1.5% n-dodecyl-β-d-maltopyranoside (βDDM; Anatrace), supplemented with 10 μg/mL DNase I and 1x cOmplete™ EDTA-free protease inhibitor cocktail (Roche) ...
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bioRxiv - Biochemistry 2020Quote: ... protein sample was spun down at 290,000 x g for 20 min and mixed with either n-octyl-β-D-glucopyranoside (OG; Anatrace) or n-nonyl-β-D-glucoside (NG ...
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bioRxiv - Biochemistry 2020Quote: ... with or without 1.5 mg/ml cardiolipin was prepared in water and solubilized using 8% (w/vol) n-octyl-β-D-maltopyranoside (Anatrace). Protein that had been purified by SEC in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... with 1 % (v/v) membrane protease inhibitors and 1.0 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM) 40:1 (w/w) CHS (Anatrace). After centrifugation ...
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bioRxiv - Biophysics 2021Quote: A total of 4.0 μl of 20 μM TpCorC protein in 20 mM HEPES (pH 7.5) containing 0.03% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, USA) and either 150 mM NaCl or 150 mM KCl was mixed with 0.5 μl of 2 mM EDTA ...
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bioRxiv - Biophysics 2022Quote: ... The pellet containing the membrane fraction was solubilized in 1% N-dodecyl-β-D-maltopyranoside (DDM) and 0.1% cholesteryl hemisuccinate (CHS; Tris Salt, Anatrace) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... equilibrated with the FPLC buffer (20 mM HEPES, pH7.5, 150 mM NaCl) plus 1 mM (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace).
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bioRxiv - Biophysics 2022Quote: ... The protein was extracted by adding 50 mM n-Dodecyl-β-D-maltopyranoside with 0.25% cholesteryl hemisuccinate (Anatrace, D310-CH210) and nutating for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Cells membranes were isolated by ultracentrifugation at 200,000 x g (45 min) and solubilised with 40 mM n-dodecyl-β-D-maltopyranoside (DDM, Anatrace). Solubilised membranes were incubated with Ni-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... the transfected 293T cells were harvested and lysed in PBS buffer containing 0.3 % n-decyl-β-D-maltopyranoside (DDM, Anatrace). Cell lysates were incubated with Protein A/G PLUS Agarose (Santa Cruz ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was incubated for 1 h at room temperature and the complex was solubilized from the membrane using 0.5% (w/v) n-dodecyl-β-d-maltoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Giβ1ψ2 G-protein were lysed in hypotonic buffer and G-protein was extracted in a buffer containing 1% sodium cholate and 0.05% n-dodecyl-β-D-maltoside (DDM, Anatrace). Detergent was exchanged from cholate/DDM to DDM on Ni Sepharose column ...
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bioRxiv - Biophysics 2023Quote: ... the PSII-enriched membranes (0.5 mg Chl/mL) were solubilized with 1.0% (w/v) α-DDM (n-dodecyl-α-D-maltopyranoside, Anatrace) in a buffer containing 25 mM MES-NaOH (pH 6.0 ...
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bioRxiv - Microbiology 2023Quote: ... 7[mM NMG (pH 8.0)) and solubilized by adding 40[mM DM (n-Decyl-β-D-Maltoside) (Sol-Grade from Anatrace). KtrB alone ...
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction pellets were solubilized in solubilization buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2% n-dodecyl-beta-d-maltopyranoside (DDM) (Anatrace, USA) with 1 mM PMSF ...
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bioRxiv - Biophysics 2023Quote: ... membranes at a protein concentration of ∼4 mg/ml were solubilized with 1% (w:v) n-dodecyl-β-d-maltopyranoside (DDM) (Anatrace) and 0.1% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were solubilized by incubating with an addition of 1% (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace) and 0.2% (w/v ...
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Structural insight into guanylyl cyclase receptor hijacking of the kinase–Hsp90 regulatory mechanismbioRxiv - Biochemistry 2023Quote: ... Membranes were thawed and solubilized with the addition of 1% n-dodecyl β-D-maltoside (DDM) and 0.1% cholesteryl hemisuccinate (CHS) (10:1) (Anatrace). Debris and unsolubilized membranes were pelleted by centrifugation at 46,000 x g ...
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bioRxiv - Biophysics 2023Quote: ... Protein was extracted via the addition of n-Dodecyl-β-D-maltopyranoside (DDM) with cholesteryl hemisuccinate (CHS) (Anatrace, D310-CH210) to a final concentration of 50 mM DDM / 4 mM CHS and rotated for 1 hour at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and receptor was subsequently extracted from membranes in a volume of 50 ml by addition of 2× solubilisation buffer (100 mM HEPES pH 7.5, 250 mM NaCl, 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Biophysics 2024Quote: ... by addition of 2× solubilization buffer (100 mM HEPES pH 7.5, 250 mM NaCl, 1 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... using a Superdex 200 26/60 (Cytiva) was adjusted from 0.05 % (w/v) n-decyl-β-D-thiomaltoside (DTM; Anatrace) to 0.1 % (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... Beads were washed for 5 times with RIPA buffer and proteins subsequently eluted using UDM (n-Undecyl-Beta-Maltoside, Anatrace) lysis buffer (0.05% UDM ...
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bioRxiv - Biochemistry 2024Quote: ... The membrane pellet was solubilized in buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 1% (w/v) n-dodecyl-β-D-maltoside (DDM, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were lysed by cryogenic milling and then solubilized using the detergent n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, solgrade). CST was purified from the clarified lysate using Talon resin (Clontech ...
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bioRxiv - Biophysics 2022Quote: ... and the membrane pellet was resuspended in Lysis buffer and the protein was solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.04% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biochemistry 2020Quote: AcrB was extracted from homogenized membranes by overnight incubation with 1 % (w/v) n-dodecyl-β-D-maltoside (DDM) detergent (Anatrace) at 4 °C with gentle agitation ...
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bioRxiv - Cell Biology 2021Quote: ... Two membrane pellets (corresponding to half a fermenter growth) were thawed and solubilized with addition of n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) to 1% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 200 μg/ml purified proteins were added into liposomes after incubation with 1% n-octyl-ß-D-glucopyranoside (ß-OG, Anatrace) at 4 °C for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2022Quote: ... for 30 min at 4 °C and then solubilized by addition of 2× buffer (50 mM HEPES, 500 mM NaCl, 2 %w/v n-dodecyl-β-D-maltopyranoside (DDM; Anatrace), 0.4 %w/v cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
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bioRxiv - Microbiology 2022Quote: ... and extracted with detergent by adding drop-wise a 10% (w/v) stock solution of n-dodecyl β-D-maltoside (DDM, Anatrace) to a final concentration of 1.3% (w/v) ...
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bioRxiv - Immunology 2020Quote: ... RBD or S1-binding clones were moved into the ReD cell-display platform (Beasley et al. 2015) and cells were membrane permeabilized using 0.5% n-octyl b-d-thioglucopyranoside (Anatrace, Cat: 0314) and labeled for FACS using either SARS-CoV-2 RBD-Fc (Sino Biological ...
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bioRxiv - Plant Biology 2022Quote: ... Then the suspension was incubated in the buffer with additional l.5 % (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) at 4 °C for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed with the Microfluidizer LM20 (Microfluidics) cell disruptor and lysate was incubated with 1% N-Dodecyl-β-D-maltoside (Anatrace) for 1 1hour at 4°C with rocking ...
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bioRxiv - Microbiology 2024Quote: ... and pellets were dissolved in solubilization buffer (20 mM Tris, 150 NaCl, 15% glycerol, 3% (% w/v) n-dodecyl-β-D-maltoside (DDM; Anatrace)) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The mixture was transferred to 4 °C and allowed to equilibrate for 20 minutes before solubilization was initiated by addition of a final concentration of 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), 0.2% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [HN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biochemistry 2022Quote: HEK293F cells (for SBA clarified lysate preparation) and HEK293T cells (for expression analysis by Western Blot) were solubilized with n-Dodecyl-β-d-maltoside (DM) detergent (Anatrace) to form micelles around membrane proteins and maintain GPCR and RAMP structure and complex formation ...
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bioRxiv - Biophysics 2023Quote: ... the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [TN buffer + 1% (w/v) n-dodecyl β-D-maltoside (DDM; #D310S, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biochemistry 2023Quote: Frozen membranes were thawed in a tepid water bath and solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM; Anatrace) with mixing for ∼1 h ...
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bioRxiv - Biophysics 2023Quote: ... washed once with PBS and were resuspended with 500 μL buffer A containing 1% N-Dodecyl-β-D-maltoside (DDM, Anatrace), 0.1% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was then incubated at room temperature (RT) for 1 hour and the membranes were solubilised by adding 1% (w/v) n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace), and 0.2% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... which were solubilized in solubilization buffer (150 mM NaCl, 20 mM Tris pH 8.0, 1% n-Dodecyl-β-D-Maltopyranoside (Anatrace, D310) and 0.2% Cholesteryl Hemisuccinate Tris salt (Anatrace ...
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bioRxiv - Microbiology 2024Quote: ... and pellets were dissolved in solubilization buffer (20 mM Tris, 150 NaCl, 15% glycerol, 2% (w/v) n-dodecyl-β-D-maltoside (DDM; Anatrace) and 0.2% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... SLC45A4 was purified to homogeneity using standard immobilised affinity chromatography (IMAC) protocols in n-either dodecyl-β-d-maltopyranoside (DDM) detergent (Anatrace) with cholesterol hemisuccinate (5:1 ratio DDM ...
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bioRxiv - Plant Biology 2024Quote: ... thylakoid membranes (400–800 µg Chl at 0.5 mg Chl/mL) were solubilized with 1% (w/v) n-dodecyl-α-D-maltoside (α-DDM) (Anatrace) for 30 min with gentle agitation ...