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Citations for anatrace :
51 - 100 of 120 citations for 8 CHLORO 2 THIEN 2 YLQUINOLINE 4 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... protein was extracted with 2% (w/v) decyl-ý-D-maltopyranoside (DM; Anatrace, Maumee, OH) for 2 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and 1mg/ml Amphipol 8-35 (Anatrace). Buffer exchange into TBS pH7.4 and 1 mg/ml Amphipol 8-35 using Amicon Ultra-4 centrifugation filters with a 100kDa cutoff (Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorinated fos-choline 8 (Anatrace, cat# F300F) was added to T ...
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bioRxiv - Biochemistry 2020Quote: TRPC4 membranes were solubilized for 2 hr in buffer B supplemented with 1% LMNG (Anatrace #NG310). Then a protocol similar to that used for DDM purification was followed ...
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bioRxiv - Biochemistry 2020Quote: Membranes were solubilized for 2 hr in buffer B supplemented with 1% dodecyl maltoside (Anatrace #D310). Insoluble material was removed by ultracentrifugation for 1 hr in a Beckman Coulter Type 70 Ti rotor at 40,000 rpm ...
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bioRxiv - Biochemistry 2019Quote: ... then treated with 1 uL of sodium 2-sulfonatoethyl methanethiosulfonate (MTSES, Anatrace, 10x stock in water) to a working concentration of 100 uM ...
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bioRxiv - Molecular Biology 2023Quote: ... Washed membranes were resuspended in solubilisation buffer (25 mM PIPES, 300 mM NaCl, 2% CHAPSO (Anatrace), 5% glycerol ...
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bioRxiv - Biochemistry 2023Quote: ... Infected Hi5 cells were collected at 72 hr post infection and lysed in 2% CHAPSO (Anatrace) buffer (25 mM Pipes pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... 3 mM fluorinated Fos-choline 8 (Anatrace, USA) was added to improve particle distribution in vitreous ice ...
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bioRxiv - Biophysics 2021Quote: ... then incubated for 1 hour in the same resuspension buffer supplemented with 2 % DDM (Anatrace, OH, USA) and 0.1 mg/ml cardiolipin ...
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bioRxiv - Biochemistry 2022Quote: ... Resuspended pellets were lysed by sonication and extracted with 2% n-Decyl-β-D-Maltopyranoside (DM) (Anatrace) for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The dried film was then resuspended in buffered water with 2% (w/v) β-n-dodecylmaltoside (Anatrace) and sonicated (Branson sonifier ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL of solubilization solution supplemented with the 2 % 6-Cyclohexyl-1-Hexyl-β-D-Maltoside (Cymal6, Anatrace) was added gently to the membrane solution and the sample incubated at RT for 30 min under moderate shaking ...
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bioRxiv - Biophysics 2019Quote: ... A column was packed with the Sepharose beads and washed with Wash Buffer (20 mM HEPES pH 8.0, 150 mM NaCl, 2 mM TCEP) containing 0.006% DMNG (Anatrace). TRPV2 was eluted with Wash Buffer containing 0.006% DMNG and 3 mg/ml 1D4 peptide (GenScript USA ...
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bioRxiv - Biophysics 2020Quote: ... The mutant CLC-ec1 proteins were extracted with n-decyl-β-D-maltopyranoside (DM, Anatrace; 2% (w/v)) and allowed to bind to cobalt resin (Talon ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of 0.6 mg/mL LayV G or 2 mg/ml LayV G with 0.01% fluorinated octyl-maltoside (Anatrace) detergent were applied onto a freshly glow discharged 2.0/2.0 UltraFoil79 grid (200 mesh) ...
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bioRxiv - Biochemistry 2021Quote: ... and fluorinated fos-choline 8 (FC8) were from Anatrace. Cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biochemistry 2019Quote: ... and then resuspended to 100 μl with solubilization buffer (85 mM NaCl, 17 mM Tris, pH 7.5 and 2% sodium dodecanoyl sarcosine (Anatrace)) ...
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bioRxiv - Cell Biology 2021Quote: ROS of 50 bovine retinas were isolated as described previously and suspended in Extraction Buffer (20 mM BTP, pH 7.9, 10% glycerol, 300 mM NaCl, 1 mM TCEP) containing 2% LMNG (Anatrace) (Papermaster ...
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bioRxiv - Biophysics 2022Quote: ... 8% (wt/vol) n-octyl-b-D-maltopyranoside (OM, Anatrace) was added to solubilize the lipids ...
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bioRxiv - Biophysics 2022Quote: ... and 2.5 mM fluorinated fos-choline-8 (Anatrace Cat# F300F), a detergent that does not interact with hydrocarbons ...
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bioRxiv - Biophysics 2023Quote: ... The addition of 0.75 mM fluorinated Fos-choline-8 (Anatrace) resulted in even ice distribution.
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) using a Dounce homogenizer and this membrane suspension was solubilized with 1% dodecyl-maltoside (DDM, Anatrace) and 0.1% cholesteryl hemi-succinate (CHS ...
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction pellets were solubilized in solubilization buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2% n-dodecyl-beta-d-maltopyranoside (DDM) (Anatrace, USA) with 1 mM PMSF ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane pellets were solubilized in 1 ml lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 10% glycerol, protease inhibitor cocktail, 2% UDM (Anatrace U300LA) on a rotary shaker for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... 1,2-Dimyristoyl-sn-glycerol-3-phosphocholine (DMPC) and 3((3-cholamidopropyl)- dimethylammonio)-2-hydroxy-1-propanesulphonate (CHAPSO) were purchased from Anatrace. Sodium dodecyl sulfate (SDS ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were solubilized by douncing in solubilization buffer (20 mM HEPES pH 8.0, 300 mM NaCl, 2% DDM detergent (Anatrace D310S), 50 mM glutamate ...
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bioRxiv - Biophysics 2020Quote: ... 10 μg of OlZAC (10 μl) was mixed with 200 μg of NAPol (Anatrace, dissolved in 2 µl of buffer B) and incubated for 16 hours ...
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bioRxiv - Biophysics 2020Quote: ... The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace, Maumee, OH) and incubating for 1 h at 4° C ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were solubilized by addition of an equal volume of ACA 750 containing 2% n-dodecyl β-D-maltoside (β-DM, Anatrace) for a final concentration of 0.5 mg mL−1 chlorophyll and 1% β-DM ...
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bioRxiv - Biophysics 2021Quote: ... proteins were overexpressed in BL21-AI E.coli competent cells and extracted into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris(2-carboxyethyl)phosphine ...
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bioRxiv - Biophysics 2023Quote: ... coli cells transformed with the expression plasmid were lysed by sonication and the protein extracted from membrane fragments into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris (2-carboxyethyl ...
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bioRxiv - Cell Biology 2023Quote: ... 200 mM NaCl, 2 mM MgAc2, 1x cOmpleteTM EDTA-free Protease Inhibitor Cocktail [Roche], 1% [w/v] glyco-diosgenin [GDN; Anatrace], 1 mM DTT) at a ratio 6.8 ml solubilization buffer per 1 g cell pellet ...
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bioRxiv - Biochemistry 2020Quote: Purified samples were supplemented with 3 mM fluorinated Fos-Choline-8 (FFC8; Anatrace) before plunge freezing ...
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bioRxiv - Biophysics 2020Quote: 3.5 μL of protein-embedded nanodiscs supplemented with 1.5 mM Fos-Choline-8 (Anatrace) was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3 ...
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bioRxiv - Biophysics 2019Quote: ... 4 mM Cholesteryl Hemisuccinate (CHS, Anatrace) and 2 μM RTx and incubated at 4° C for 1 hour ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... 3.5 μL of GltPh-containing nanodiscs (7 mg/mL) supplemented with 1.5 mM fluorinated Fos-Choline-8 (Anatrace) were applied to a glow-discharged UltrAuFoil R1.2/1.3 300-mesh gold grid (Quantifoil ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of 8 mg/ml complex with 3 mM CHAPSO or 0.01% fluorinated octyl-maltoside (FOM) (Anatrace) were applied onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysate was dounce homogenized (8 strokes with the A pestle) and a 1% Dodecyl maltoside (DDM)/0.2% Cholesteryl hemisuccinate (CHS) (Anatrace) w:w mixture was added for protein solubilization ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 8 column buffers of GTBS (50 mM Tris-HCl, 150 mM NaCl, 0.006 % (w/v) glyco-diosgenin (GDN, Anatrace), pH 8.0) ...
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bioRxiv - Biophysics 2021Quote: ... purified protein at a concentration of 6–7 mg/mL was mixed with 2.9 mM Fluorinated Fos-Choline-8 (FFC8) (Anatrace) immediately prior to grid preparation ...
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bioRxiv - Biophysics 2022Quote: ... Cryo-EM grids of anaerobically-purified FHL were prepared similarly within the anaerobic chamber except that 0.5x CMC fluorinated fos-choline-8 (Anatrace) was added immediately before the sample was applied to the grid ...
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bioRxiv - Biochemistry 2024Quote: 3.5 uL of nhTMEM16-containing nanodiscs (3-7mg ml-1) supplemented with 1.5 or 3 mM Fos-Choline-8-Fluorinated (Anatrace) was applied to a glow-discharged UltrAuFoil R1.2/1.3 300-mesh gold grid (Quantifoil ...
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bioRxiv - Biochemistry 2024Quote: ... Nanodiscs were formed with a mixture of phospholipids (48% POPC, 32% POPE, 12% DOPS, 8% cardiolipid (w/w)) (Anatrace). The cell-free synthesis protocol was detailed previously [32,36,70] ...
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bioRxiv - Biochemistry 2020Quote: ... with or without 1.5 mg/ml cardiolipin was prepared in water and solubilized using 8% (w/vol) n-octyl-β-D-maltopyranoside (Anatrace). Protein that had been purified by SEC in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... 2.7 µL of sample was mixed with 0.3 µL of fluorinated fos-choline-8 solution (Anatrace; final concentration 1.5 mM) and applied to UltrAuFoil 0.6/1 300 mesh gold grids (Quantifoil) ...
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bioRxiv - Biochemistry 2021Quote: ... 6 mM amino acid mixture (average concentration of each amino acid 0.3 mM) and 0.1% (w/v) MNG-3 (Maltose Neopentyl Glycol-3, Anatrace). The feeding buffer contained 1x SUB-AMIX buffer (CellFree Sciences Japan) ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 8 column buffers of GTBS (50 mM Tris HCl, 150 mM NaCl, 0.006% [w/v] glyco-diosgenin [GDN; Anatrace], pH 8.0). Ycf1p was eluted with 6 column volumes of GTBS containing 150 μg/mL 3×FLAG peptide ...
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bioRxiv - Immunology 2023Quote: ... the complex was extracted from the membrane with 0.8% (w/v) lauryl maltose neopentylglycol (LMNG; Anatrace, CAS. No. 1423315-02-8) and 0.1% (w/v ...