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Citations for anatrace :
51 - 81 of 81 citations for 6 Hydroxy 2 methylindole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Protein extraction was carried out with 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace #D322S25GM) for 2 hr at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... protein was extracted with 2% (w/v) decyl-ý-D-maltopyranoside (DM; Anatrace, Maumee, OH) for 2 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: TRPC4 membranes were solubilized for 2 hr in buffer B supplemented with 1% LMNG (Anatrace #NG310). Then a protocol similar to that used for DDM purification was followed ...
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bioRxiv - Biochemistry 2020Quote: Membranes were solubilized for 2 hr in buffer B supplemented with 1% dodecyl maltoside (Anatrace #D310). Insoluble material was removed by ultracentrifugation for 1 hr in a Beckman Coulter Type 70 Ti rotor at 40,000 rpm ...
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bioRxiv - Biochemistry 2019Quote: ... then treated with 1 uL of sodium 2-sulfonatoethyl methanethiosulfonate (MTSES, Anatrace, 10x stock in water) to a working concentration of 100 uM ...
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bioRxiv - Molecular Biology 2023Quote: ... Washed membranes were resuspended in solubilisation buffer (25 mM PIPES, 300 mM NaCl, 2% CHAPSO (Anatrace), 5% glycerol ...
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bioRxiv - Biochemistry 2023Quote: ... Infected Hi5 cells were collected at 72 hr post infection and lysed in 2% CHAPSO (Anatrace) buffer (25 mM Pipes pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... then incubated for 1 hour in the same resuspension buffer supplemented with 2 % DDM (Anatrace, OH, USA) and 0.1 mg/ml cardiolipin ...
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bioRxiv - Biochemistry 2022Quote: ... Resuspended pellets were lysed by sonication and extracted with 2% n-Decyl-β-D-Maltopyranoside (DM) (Anatrace) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... at ~5 mg/mL in SEC Buffer containing 2 mM fluorinated fos-choline-8 (Anatrace, cat# F300F), was applied to glow discharged Quantifoil R1.2/1.3 grids ...
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bioRxiv - Biochemistry 2023Quote: ... The dried film was then resuspended in buffered water with 2% (w/v) β-n-dodecylmaltoside (Anatrace) and sonicated (Branson sonifier ...
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bioRxiv - Biophysics 2019Quote: ... A column was packed with the Sepharose beads and washed with Wash Buffer (20 mM HEPES pH 8.0, 150 mM NaCl, 2 mM TCEP) containing 0.006% DMNG (Anatrace). TRPV2 was eluted with Wash Buffer containing 0.006% DMNG and 3 mg/ml 1D4 peptide (GenScript USA ...
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bioRxiv - Biophysics 2020Quote: ... The mutant CLC-ec1 proteins were extracted with n-decyl-β-D-maltopyranoside (DM, Anatrace; 2% (w/v)) and allowed to bind to cobalt resin (Talon ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of 0.6 mg/mL LayV G or 2 mg/ml LayV G with 0.01% fluorinated octyl-maltoside (Anatrace) detergent were applied onto a freshly glow discharged 2.0/2.0 UltraFoil79 grid (200 mesh) ...
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bioRxiv - Biophysics 2019Quote: ... at 4 °C for 2 h and then washed with 30 bed volumes of lysis buffer added 0.05% GDN (Anatrace). The protein was eluted with lysis buffer added 0.05% GDN and 300 μg ml-1 Flag peptide (Genscript) ...
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bioRxiv - Biochemistry 2019Quote: ... and then resuspended to 100 μl with solubilization buffer (85 mM NaCl, 17 mM Tris, pH 7.5 and 2% sodium dodecanoyl sarcosine (Anatrace)) ...
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bioRxiv - Cell Biology 2021Quote: ROS of 50 bovine retinas were isolated as described previously and suspended in Extraction Buffer (20 mM BTP, pH 7.9, 10% glycerol, 300 mM NaCl, 1 mM TCEP) containing 2% LMNG (Anatrace) (Papermaster ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) using a Dounce homogenizer and this membrane suspension was solubilized with 1% dodecyl-maltoside (DDM, Anatrace) and 0.1% cholesteryl hemi-succinate (CHS ...
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bioRxiv - Biophysics 2023Quote: ... The membrane fraction pellets were solubilized in solubilization buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2% n-dodecyl-beta-d-maltopyranoside (DDM) (Anatrace, USA) with 1 mM PMSF ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane pellets were solubilized in 1 ml lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 10% glycerol, protease inhibitor cocktail, 2% UDM (Anatrace U300LA) on a rotary shaker for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were solubilized by douncing in solubilization buffer (20 mM HEPES pH 8.0, 300 mM NaCl, 2% DDM detergent (Anatrace D310S), 50 mM glutamate ...
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bioRxiv - Biochemistry 2024Quote: ... The resuspension was solubilized at 4 °C for 4 h with 2% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... 10 μg of OlZAC (10 μl) was mixed with 200 μg of NAPol (Anatrace, dissolved in 2 µl of buffer B) and incubated for 16 hours ...
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bioRxiv - Biophysics 2020Quote: ... The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace, Maumee, OH) and incubating for 1 h at 4° C ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were solubilized by addition of an equal volume of ACA 750 containing 2% n-dodecyl β-D-maltoside (β-DM, Anatrace) for a final concentration of 0.5 mg mL−1 chlorophyll and 1% β-DM ...
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bioRxiv - Biophysics 2021Quote: ... proteins were overexpressed in BL21-AI E.coli competent cells and extracted into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris(2-carboxyethyl)phosphine ...
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bioRxiv - Biochemistry 2023Quote: ... the receptor was solubilized for 2 hrs at 4°C with continual stirring in a solution of 0.5% L-MNG (Anatrace, Cat. no: NG310) and 0.1% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... coli cells transformed with the expression plasmid were lysed by sonication and the protein extracted from membrane fragments into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris (2-carboxyethyl ...
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bioRxiv - Cell Biology 2023Quote: ... 200 mM NaCl, 2 mM MgAc2, 1x cOmpleteTM EDTA-free Protease Inhibitor Cocktail [Roche], 1% [w/v] glyco-diosgenin [GDN; Anatrace], 1 mM DTT) at a ratio 6.8 ml solubilization buffer per 1 g cell pellet ...
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bioRxiv - Biochemistry 2022Quote: ... Drops of 450 nL of 10 mg/mL protein in SEC buffer and 150 nL of MCSG-1 H10 (Anatrace; 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...