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Citations for Alfa Aesar :
1 - 50 of 119 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... ABTS substrate (KPL, Maryland WA), TRIS-buffered saline (TBS, 10X) pH=7.4 for western blot (Alfa Aesar, Tewksbury, MA) and 10% neutral buffered formalin (Leica biosystems ...
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bioRxiv - Immunology 2022Quote: EV and cellular protein (20μg for Calnexin and 5μg for GAPDH blots) was mixed with reducing sample buffer (Cat. No. J61337, Alfa Aesar), heated at 95°C for 5min and separated on a Bolt 4-12% Bis-Tris gel (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... 6x Laemmli buffer (Alfa Aesar) containing BME was added and beads were boiled for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then equalized and 6x sodium dodecyl sulfate buffer (SDS sample buffer; Alfa Aesar, Wood Hill ...
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bioRxiv - Immunology 2022Quote: ... column running TBS buffer (Alfa Aesar)) ...
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bioRxiv - Neuroscience 2022Quote: ... Laemmli SDS sample buffer (Alfa Aesar) was added to lysates which were boiled at 95°C for 5 minutes prior to SDS-PAGE ...
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bioRxiv - Immunology 2022Quote: ... running in TBS buffer (Alfa Aesar). Fractions corresponding to Coxsackievirus A21 particles were pooled and concentrated to 0.5mg/ml using Amicon Ultra centrifugal filter units with 100 kDa cutoff (Millipore Sigma).
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bioRxiv - Synthetic Biology 2024Quote: ... Laemmli SDS sample buffer (Alfa Aesar) was added to supernatants and incubated at 65 °C for 10 min ...
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bioRxiv - Genomics 2024Quote: ... containing 4µl of lysis buffer consisting of 16.67mM TAPS-NaOH buffer (pH 8.5, Alfa Aesar, Cat: J63268), 8.33mM MgCl2(Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: ... the buffer contained propidium iodide (Alfa Aesar). Cells and buffer were incubated at room temperature (20−22°C ...
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bioRxiv - Biophysics 2020Quote: ... 10x TAE buffer was purchased from Alfa Aesar. Magnesium acetate tetrahydrate (molecular biology grade ...
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bioRxiv - Cell Biology 2023Quote: ... 6x Laemmli SDS sample buffer (J60660, Alfa Aesar) (with β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein and varying amounts of sodium dodecyl sulfate buffer (SDS sample buffer; Alfa Aesar, Wood Hill, MA, USA) supplemented with beta-mercapto-ethanol and water were used in each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein pellet was dissolved in RIPA buffer and then 20 μL of 4× reducing Laemmli SDS sample loading buffer (Cat. No. J63615, Alfa Aesar) was added and heated at 95 °C for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... mixed with 6x SDS Laemmli reducing buffer (Alfa Aesar), then heat-denatured at 85 °C for 10 min and kept at room temperature until needed ...
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bioRxiv - Biochemistry 2020Quote: ... in sterile-filtered HEPES-Tyrode Buffer (HTB) (Alfa Aesar) supplemented with 0.01% Plurionic F-127 (Molecular Probes ...
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bioRxiv - Neuroscience 2023Quote: Cell cultures were harvested in RIPA buffer (Alfa Aesar) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were lysed at RT for 10 min using freshly prepared lysis buffer: RIPA buffer with EDTA and EGTA (Alfa Aesar/Thermo Fisher Scientific) + 5% SDS + 2x cOmplete protease inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... Pelleted cells were treated with RBC lysis buffer (Alfa Aesar), counted by an automated cell counter (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: Cells were harvested and resuspended in RIPA buffer (Alfa Aesar) supplemented with protease and phosphatase inhibitors (ThermoScientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar) and denatured at 95°C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... The gel was run in 1X TBE buffer (Alfa Aesar) at 4 V/cm ...
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bioRxiv - Cell Biology 2021Quote: Cells were harvested and resuspended in RIPA buffer (Alfa Aesar) supplemented with protease and phosphatase inhibitors (ThermoScientific ...
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bioRxiv - Biochemistry 2021Quote: Cell pellets were lysed in 2x Laemmli buffer (Alfa Aesar) and performed as described previously 12 with the following antibodies ...
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bioRxiv - Bioengineering 2023Quote: Tissues were lysed in commercial RIPA buffer (Alfa Aesar J63306), with protease inhibitor (Millipore-Sigma P8340 ...
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bioRxiv - Biochemistry 2023Quote: ... 20 μL of Laemmli SDS sample loading buffer (Alfa Aesar) was added to quench the reaction and proteins were analyzed by Western Blot.
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were prepared using NP-40 buffer (Alfa Aesar). Quantification of human/mouse chemokines in tissue culture supernatants and tissue homogenates was performed using a commercially available human CXCL1/GRO alpha ...
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bioRxiv - Cell Biology 2023Quote: ... 200 µl of RIPA lysis buffer (Alfa Aesar, Cat# J63306) supplemented with 1x protease inhibitor cocktail (GenDepot ...
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bioRxiv - Biochemistry 2023Quote: ... 20 μL of Laemmli SDS sample loading buffer (Alfa Aesar) was added to quench the reaction and proteins were analyzed by Western Blot ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was composed of equal amounts of protein in addition to varying amounts of sodium dodecyl sulfate buffer (SDS sample buffer; Alfa Aesar, Ward Hill, MA, USA) supplemented with beta-mercapto-ethanol and water ...
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bioRxiv - Biophysics 2022Quote: ... citrate blood was resuspended in a viscosity-adjusted measurement buffer (phosphate saline buffer without Mg2+ and Ca2+ (PBS−) containing 0.5% (w/v) methyl cellulose (4000 cPs, Alfa Aesar 036718.22, CAS# 9004-67-5); adjusted in HAAKE Falling Ball Viscometer type C (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatants were combined with Laemmli SDS sample buffer (Alfa Aesar) and incubated at 65°C for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were boiled in Laemmli SDS sample buffer (Alfa Aesar) for 10 min and separated by Mini-Protean TGX Gels 4-20 % (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... and then blocked with 200 µL/well Blocking Buffer (Alfa Aesar) at 4°C overnight ...
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bioRxiv - Bioengineering 2020Quote: ... Sodium phosphate buffer (0.2 M, pH 8.5) was purchased from Alfa Aesar. Clear silicone sealant was purchased from Loctite ...
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bioRxiv - Molecular Biology 2022Quote: ... the agarose beads were boiled in Laemmli SDS sample buffer (Alfa Aesar) for western blot analysis with rabbit anti-Myc antibody (CST ...
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bioRxiv - Molecular Biology 2022Quote: ... the agarose beads were boiled in Laemmli SDS sample buffer (Alfa Aesar) for western blot analysis with rabbit anti-eNOS antibody (CST ...
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bioRxiv - Biophysics 2020Quote: ... and zyxin were buffer-exchanged into 100 mM NaF (Alfa Aesar, A13019) dissolved in phosphate buffer (pH 7.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... in 30 µl mg-1 RIPA buffer (Alfa Aesar, Haverhill, MA, USA) containing 10 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: Citrate buffer stabilized AuNPs (10 nm diameter) was purchased from Alfa Aesar (Thermo Fisher Scientific India Private Limited) ...
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bioRxiv - Bioengineering 2022Quote: Cells on PAA gel coverslips were lysed with RIPA buffer (Alfa Aesar). For the ELISA ...
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bioRxiv - Genetics 2022Quote: ... 2-(N-morpholino)ethanesulfonic acid (MES) buffer solution was from Alfa Aesar.
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bioRxiv - Cancer Biology 2023Quote: ... and dissolved in 50 mM HEPES pH 8.5 buffer (Alfa Aesar #J63218).
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bioRxiv - Bioengineering 2021Quote: ... BMMs were harvested and lysed with ice-cold RIPA lysis buffer (Alfa Aesar) supplemented with Halt Protease and Phosphatase Inhibitors (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... in 0.1 M phosphate buffer at pH 7.4 with 50% heptane (Alfa Aesar) for 30 min and manually devitellinized (TLR stains ...
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bioRxiv - Immunology 2023Quote: ... equal amounts of protein were boiled in 6X Laemmli sample buffer (Alfa Aesar) containing 5% β-mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: ... the beads were treated with 6x Laemmli SDS sample buffer (J60660, Alfa Aesar) (with 10% β-mercaptoethanol) ...
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bioRxiv - Neuroscience 2024Quote: ... The final pellet was dissolved in 1x TE buffer (Alfa Aesar, #J60738-AK), which became the sarkosyl insoluble fraction (P3).
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bioRxiv - Microbiology 2020Quote: ... with 25 mM final concentration of PIPES buffer (Alfa Aesar, 0.5 M, pH 7.5); uracil added to this medium was dissolved in ddH2O (Millipore Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... The pelleted stromal vascular fraction (SVF) was treated with RBC lysis buffer (Alfa Aesar, Tewksbury ...