Labshake search
Citations for Alfa Aesar :
151 - 181 of 181 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: ... The infection was terminated by washing the cells with PBS and fixing them with 4% paraformaldehyde (Alfa Aesar) for 15 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were trypsinized and washed twice with PBS and lysed in 2x Laemmli Sample buffer (Alfa Aesar, J61337). For cytosolic lysate ...
-
bioRxiv - Molecular Biology 2021Quote: ... the coverslips were washed 3 times with PBS and cells fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Heysham, England) in PBS for 20 minutes ...
-
bioRxiv - Biophysics 2020Quote: ... the cells were suspended in 100 μL viscosity-adjusted measurement buffer (0.5% [w/v] methylcellulose [4000 cPs, Alfa Aesar, Germany] in phosphate-buffered saline without Mg2+ and Ca2+ ...
-
bioRxiv - Immunology 2020Quote: ... Subsequently the cells were resuspended in TBS buffer (25 mM Tris + 2.7 mM KCl + 137 mM NaCl, pH 7.4; Alfa Aesar / Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were washed three times with 200μL of FACS buffer and fixed with 200μL of 4% PFA (Alfa Aesar) for 15 mins at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... tubing and pipette tips used to handle wounded cells were treated with 3% Pluronic F-68 (J6608736, Alfa Aesar) in deionized (DI ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were incubated with 10 μM of the blocking agent 7-bromoheptanol (Merrill et al. 2019) (Alfa Aesar, H54762), for 2 h ...
-
bioRxiv - Bioengineering 2023Quote: ... the cancer cell lines MCF7 and MDA-MB-231 were fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Haverhill, MA) for 15-30 min at room temperature following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... The medium was then replaced by the normal cell culture medium supplemented with 2 mM L-methionine (Alfa Aesar J61904) for 0-3 hr at 37°C for the chase ...
-
bioRxiv - Immunology 2020Quote: ... BL21 cells were spun down (3000 RPM, 30 min, 4 °C) and resuspended in Tris-buffered saline (TBS, Alfa Aesar) containing cOmplete™ protease inhibitor cocktail (Sigma Millipore) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with isopropanol (60%) for 5 minutes and then with ORO solution (ORO, 2mg/mL, Alfa Aesar, A12989) for 20 min ...
-
bioRxiv - Biophysics 2020Quote: ... Hydrated protein powders were then sealed in aluminum flat neutron cells (sample thickness=0.3 mm) using 1 mm indium wire from Alfa Aesar. Samples were weighed before and after the neutron experiment to verify that they were correctly sealed and no water was lost ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cas9 and BFP expression induction was achieved by treatment of the cells for 3 days with doxycycline (Dox) (1µg/ml) (Hyclate, Alfa Aesar) and single cell clones were seeded by sorting BFP positive cells using the BD FACSAria™ Cell Sorter system ...
-
bioRxiv - Cell Biology 2023Quote: ... Propidium iodide was used as a dead cell marker (1mg/ml aqueous solution; 25535-16-4, Alfa Aesar, Lancashire, UK). Cells were then sorted using fluorescence-activated cell sorting (BD Biosciences ...
-
bioRxiv - Systems Biology 2023Quote: ... HeLa cells were transfected with YFP-ATGL variants using Polyethylenimine (PEI, linear, M. W. 20,000 [Alfa Aesar, 9002-98-6]) as transfection reagent (ratio DNA:PEI = 1:5) ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were stained with alcian blue (1% w/v in 3% v/v acetic acid; Alfa Aesar; goblet cell stain), hematoxylin (0.2% w/v ...
-
bioRxiv - Biophysics 2019Quote: ... cells were suspended in 100 μl of a viscosity-adjusted measurement buffer (0.5% (w/v) methylcellulose (4000 cPs, Alfa Aesar, Germany) in phosphate saline buffer without Mg2+ and Ca2+ ...
-
bioRxiv - Microbiology 2021Quote: ... sample volume was adjusted such that approximately 107 cells were harvested and re-suspended in 1 x Hepes Buffered Saline (5X HBS; Alfa Aesar). Cells were washed and resuspended in 1 mL HBS ...
-
bioRxiv - Immunology 2019Quote: Immunofluorescence on saponin-permeabilized cells was carried out as described previously [5] except that cells were fixed in methanol-free 4% (w/v) paraformaldehyde (PFA, Alfa Aesar) in phosphate buffered saline (PBS) ...
-
bioRxiv - Developmental Biology 2022Quote: Osteogenic differentiation was induced by providing confluent cells with DMEM and 10% FBS supplemented with 10 mM sodium beta-glycerolphosphate (Cat# L03425, Alfa Aesar), 100 nM dexamethasone (Cat# D1756 ...
-
bioRxiv - Microbiology 2022Quote: ... to stain the membrane and the cell wall was labeled with 1 μg/ml of an equal mixture of vancomycin (Alfa Aesar) and a BODIPY FL conjugate of vancomycin (Invitrogen) ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were lysed in the digitonin lysis buffer (1% digitonin [Sigma-Aldrich D141], 137 mM NaCl, 10% w/v glycerol [Alfa Aesar 38988] ...
-
bioRxiv - Microbiology 2022Quote: ... 1μg gRNA and 3μg of pCTR4-2-FKS1 promoter construct (48) and cells were plated on YPD with 400μg/mL hygromycin B (Alfa Aesar, Cat# J69681). Transformants were verified with PCR spanning the joint between the promoter construct and FKS1 coding sequence and tested for response to copper by growth assay and gene expression.
-
bioRxiv - Microbiology 2022Quote: ... This was followed by fixing the remaining adherent A549 cells in the wells with 4% formaldehyde in PBS for 10 min (Alfa Aesar, UK), permeabilizing the cells with 0.2% Triton-X100 (VWR ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 × 105 cells were seeded onto glass coverslip (SLS, MIC3300) overnight before fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899) for 10min ...
-
bioRxiv - Cancer Biology 2021Quote: ... S34F and Q157R HEL cells and not-infected HEL cells were labeled in duplicate with 100 μM of the uridine analog 4-thiouridine (s4U, Alfa Aesar, Cat #AAJ60679MC) for 2 hours at 37°C in the dark ...
-
bioRxiv - Cancer Biology 2019Quote: ... and cryopreserved at 10-15×106 cells/cryovial (RPMI-1640, Corning #15-040-CV; 20% human AB serum, Valley Biomedical #HP1022; 10% DMSO, Alfa Aesar #42780).
-
bioRxiv - Biophysics 2023Quote: ... cells and beads were suspended into a 0.6 w/w% solution of methyl cellulose (MC) (4000 cPs, Alfa Aesar 036718.22, CAS 9004-67-5) dissolved in phosphate buffered saline (PBS ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were lysed at RT for 10 min using freshly prepared lysis buffer: RIPA buffer with EDTA and EGTA (Alfa Aesar/Thermo Fisher Scientific) + 5% SDS + 2x cOmplete protease inhibitor cocktail (Roche) ...
-
bioRxiv - Biophysics 2021Quote: ... or ER-mCherry-S1-2 (16 μg) and sonicated salmon sperm DNA (14 μg) were transfected into the cells with 240 μL of polyethyleneimine (#43896, Alfa Aesar, Ward Hill, MA, USA). After incubation of the cells for 24 h ...