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Citations for Alfa Aesar :
101 - 148 of 148 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... and the cells were washed with cold 1XPBS and fixed using 4% PFA (Alfa Aesar #J61899). Cells were blocked for 1 hour using a blocking buffer composed of 1XPBS ...
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bioRxiv - Microbiology 2024Quote: ... and cells were lysed using 100 µL 0.1% Triton X-100 (Alfa Aesar, Ward Hill, MA) in PBS for 5 minutes at room temperature by gentle pipetting ...
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bioRxiv - Neuroscience 2019Quote: ... NIH3T3 cells were cultured for 4 hours in the presence of 100 nM Bafilomycin A (Alfa Aesar) to block autophagy and/or in DMEM media without glutamine and serum for autophagy induction prior to downstream analysis ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in presence of ultrapure Zn sulfate monohydrate purchased from Alfa Aesar (Haverhill, MA. #1113809) at 5μM ...
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bioRxiv - Developmental Biology 2022Quote: Cells cultured in a µ-slide 8 well dish were fixed with 4% paraformaldehyde (J61899, Alfa Aesar) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: Unless otherwise stated all cell lysates were made by direct lysis in 6X Laemmli buffer (Alfa Aesar) to inactive SENPs and denature proteins followed by heating at 95 °C for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: Cells were fixed using 3.7% (v/v) formaldehyde then permeabilised using 0.1% Triton X-100 (Alfa Aesar) for 30min ...
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bioRxiv - Biophysics 2023Quote: ... 3T3 fibroblast cells were fixed for 15 min in 4% paraformaldehyde (43368; Alfa Aesar, Haverhill, MA, USA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were cultured in differentiation medium supplemented with 0.125 mM indomethacin (cat#A19910-06, Alfa Aesar), 0.5 µM dexamethasone (cat#d4902 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed for 10 minutes with room temperature (RT) 3.5% PFA (paraformaldehyde, pH 6.7; Alfa Aesar). Cells were then washed 3 x with Tris buffered saline (TBS ...
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bioRxiv - Immunology 2021Quote: ... Cells were fixed for 30 minutes at room temperature in 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899). Cells were washed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were fixed with 4% paraformaldehyde (PFA; Electron Microscopy Services) with 0.075 mg/mL saponin (Alfa Aesar,Sigma) diluted in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2019Quote: ... A 100 μL aliquot of this cell suspension was added to 900 μL of a chitin (Alfa Aesar) IO mixture (8 g/L chitin) ...
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bioRxiv - Microbiology 2020Quote: ... a 500 μl aliquot of cells in growth medium was added to 100 μl 16% paraformaldehyde (Alfa Aesar) and 20 μl of 1M NaPO4 buffer (pH 7.4) ...
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bioRxiv - Biophysics 2021Quote: ... 3T3 fibroblast and HUVEC cells were fixed for 15 min in 4% paraformaldehyde (43368; Alfa Aesar, Haverhill, MA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Genomics 2022Quote: ... The infection was terminated by washing the cells with PBS and fixing them with 4% paraformaldehyde (Alfa Aesar) for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were trypsinized and washed twice with PBS and lysed in 2x Laemmli Sample buffer (Alfa Aesar, J61337). For cytosolic lysate ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed and flash frozen with liquid N2 before being lysed with lysis buffer containing cycloheximide (Alfa Aesar). Lysate was digested with RNase I (Lucigen ...
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bioRxiv - Molecular Biology 2021Quote: ... the coverslips were washed 3 times with PBS and cells fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Heysham, England) in PBS for 20 minutes ...
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bioRxiv - Biophysics 2020Quote: ... the cells were suspended in 100 μL viscosity-adjusted measurement buffer (0.5% [w/v] methylcellulose [4000 cPs, Alfa Aesar, Germany] in phosphate-buffered saline without Mg2+ and Ca2+ ...
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bioRxiv - Immunology 2020Quote: ... Subsequently the cells were resuspended in TBS buffer (25 mM Tris + 2.7 mM KCl + 137 mM NaCl, pH 7.4; Alfa Aesar / Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed three times with 200μL of FACS buffer and fixed with 200μL of 4% PFA (Alfa Aesar) for 15 mins at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... tubing and pipette tips used to handle wounded cells were treated with 3% Pluronic F-68 (J6608736, Alfa Aesar) in deionized (DI ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated with 10 μM of the blocking agent 7-bromoheptanol (Merrill et al. 2019) (Alfa Aesar, H54762), for 2 h ...
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bioRxiv - Bioengineering 2023Quote: ... the cancer cell lines MCF7 and MDA-MB-231 were fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Haverhill, MA) for 15-30 min at room temperature following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The medium was then replaced by the normal cell culture medium supplemented with 2 mM L-methionine (Alfa Aesar J61904) for 0-3 hr at 37°C for the chase ...
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bioRxiv - Immunology 2020Quote: ... BL21 cells were spun down (3000 RPM, 30 min, 4 °C) and resuspended in Tris-buffered saline (TBS, Alfa Aesar) containing cOmplete™ protease inhibitor cocktail (Sigma Millipore) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with isopropanol (60%) for 5 minutes and then with ORO solution (ORO, 2mg/mL, Alfa Aesar, A12989) for 20 min ...
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bioRxiv - Biophysics 2020Quote: ... Hydrated protein powders were then sealed in aluminum flat neutron cells (sample thickness=0.3 mm) using 1 mm indium wire from Alfa Aesar. Samples were weighed before and after the neutron experiment to verify that they were correctly sealed and no water was lost ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 and BFP expression induction was achieved by treatment of the cells for 3 days with doxycycline (Dox) (1µg/ml) (Hyclate, Alfa Aesar) and single cell clones were seeded by sorting BFP positive cells using the BD FACSAria™ Cell Sorter system ...
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bioRxiv - Cell Biology 2023Quote: ... Propidium iodide was used as a dead cell marker (1mg/ml aqueous solution; 25535-16-4, Alfa Aesar, Lancashire, UK). Cells were then sorted using fluorescence-activated cell sorting (BD Biosciences ...
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bioRxiv - Systems Biology 2023Quote: ... HeLa cells were transfected with YFP-ATGL variants using Polyethylenimine (PEI, linear, M. W. 20,000 [Alfa Aesar, 9002-98-6]) as transfection reagent (ratio DNA:PEI = 1:5) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were stained with alcian blue (1% w/v in 3% v/v acetic acid; Alfa Aesar; goblet cell stain), hematoxylin (0.2% w/v ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in the presence of 100 microliters radio-immunoprecipitation (RIPA) buffer containing protease inhibitors (Alfa Aesar, Stoughton, MA, USA) on ice ...
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bioRxiv - Biophysics 2019Quote: ... cells were suspended in 100 μl of a viscosity-adjusted measurement buffer (0.5% (w/v) methylcellulose (4000 cPs, Alfa Aesar, Germany) in phosphate saline buffer without Mg2+ and Ca2+ ...
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bioRxiv - Microbiology 2021Quote: ... sample volume was adjusted such that approximately 107 cells were harvested and re-suspended in 1 x Hepes Buffered Saline (5X HBS; Alfa Aesar). Cells were washed and resuspended in 1 mL HBS ...
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bioRxiv - Immunology 2019Quote: Immunofluorescence on saponin-permeabilized cells was carried out as described previously [5] except that cells were fixed in methanol-free 4% (w/v) paraformaldehyde (PFA, Alfa Aesar) in phosphate buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2022Quote: Osteogenic differentiation was induced by providing confluent cells with DMEM and 10% FBS supplemented with 10 mM sodium beta-glycerolphosphate (Cat# L03425, Alfa Aesar), 100 nM dexamethasone (Cat# D1756 ...
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bioRxiv - Microbiology 2022Quote: ... to stain the membrane and the cell wall was labeled with 1 μg/ml of an equal mixture of vancomycin (Alfa Aesar) and a BODIPY FL conjugate of vancomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed in the digitonin lysis buffer (1% digitonin [Sigma-Aldrich D141], 137 mM NaCl, 10% w/v glycerol [Alfa Aesar 38988] ...
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bioRxiv - Microbiology 2022Quote: ... 1μg gRNA and 3μg of pCTR4-2-FKS1 promoter construct (48) and cells were plated on YPD with 400μg/mL hygromycin B (Alfa Aesar, Cat# J69681). Transformants were verified with PCR spanning the joint between the promoter construct and FKS1 coding sequence and tested for response to copper by growth assay and gene expression.
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bioRxiv - Microbiology 2022Quote: ... This was followed by fixing the remaining adherent A549 cells in the wells with 4% formaldehyde in PBS for 10 min (Alfa Aesar, UK), permeabilizing the cells with 0.2% Triton-X100 (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 × 105 cells were seeded onto glass coverslip (SLS, MIC3300) overnight before fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899) for 10min ...
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bioRxiv - Cancer Biology 2021Quote: ... S34F and Q157R HEL cells and not-infected HEL cells were labeled in duplicate with 100 μM of the uridine analog 4-thiouridine (s4U, Alfa Aesar, Cat #AAJ60679MC) for 2 hours at 37°C in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... and cryopreserved at 10-15×106 cells/cryovial (RPMI-1640, Corning #15-040-CV; 20% human AB serum, Valley Biomedical #HP1022; 10% DMSO, Alfa Aesar #42780).
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bioRxiv - Biophysics 2023Quote: ... cells and beads were suspended into a 0.6 w/w% solution of methyl cellulose (MC) (4000 cPs, Alfa Aesar 036718.22, CAS 9004-67-5) dissolved in phosphate buffered saline (PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were lysed at RT for 10 min using freshly prepared lysis buffer: RIPA buffer with EDTA and EGTA (Alfa Aesar/Thermo Fisher Scientific) + 5% SDS + 2x cOmplete protease inhibitor cocktail (Roche) ...
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bioRxiv - Biophysics 2021Quote: ... or ER-mCherry-S1-2 (16 μg) and sonicated salmon sperm DNA (14 μg) were transfected into the cells with 240 μL of polyethyleneimine (#43896, Alfa Aesar, Ward Hill, MA, USA). After incubation of the cells for 24 h ...