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Citations for Alfa Aesar :
101 - 134 of 134 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Bound proteins (‘output’) were eluted by boiling the beads (95 °C, 10 minutes) with 1x Laemelli sample buffer (Alfa Aesar) containing 30 mM DTT ...
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bioRxiv - Bioengineering 2019Quote: DSS-treated mice received 3 cycles of 2% (weight/volume) DSS (40,000 MW, Alfa Aesar, Thermo Fisher, France) in drinking water for 5 days ...
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bioRxiv - Cell Biology 2021Quote: ... and BAPTA (1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid) from Alfa Aesar (Tewksbury, MA). GSK101 (1 mM) ...
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bioRxiv - Microbiology 2022Quote: The synthesis began with a reaction of commercially available N-Boc-L-tyrosine (2 mmol, Alfa Aesar, 98%) and oleylamine (2.4 mmol ...
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bioRxiv - Bioengineering 2023Quote: ... coverslips were acryl-silanised submerged for 2 hrs in a 0.5% solution of 3-(Acryloyloxy)propyltrimethoxysilane (Alfa Aesar) in ethanol with 5% water ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein and varying amounts of sodium dodecyl sulfate buffer (SDS sample buffer; Alfa Aesar, Wood Hill, MA, USA) supplemented with beta-mercapto-ethanol and water were used in each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... library with an estimated diversity of 1.7×109 different clones of M13 bacteriophage is used to select peptide sequences fused to the minor coat protein (pIII) against MoS2 flakes (∼300 mesh, 99%, 10129, Alfa Aesar). Before the screening process ...
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bioRxiv - Genetics 2023Quote: ... Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) samples were prepared by boiling equal amounts of protein in 6X Laemmli sample buffer (Alfa Aesar) containing 5% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... MTT (3-[4, 5- dimethyl thiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (Alfa Aesar Chemicals Co. Ltd. Shanghai, China), fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2019Quote: ... and then crocein scarlet-acid fuchsin solution for 2 minutes (4 parts crocein scarlet – 0.1% crocein scarlet [Alfa Aesar, J66876] and 0.5% glacial acetic acid ...
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bioRxiv - Bioengineering 2019Quote: ... and then simultaneously blocked and permeabilized with 2% BSA in PBS++ with 0.1% saponin (Alfa Aesar, cat. no. A18820) for 1hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... mice were anesthetized and sacrificed by transcardial perfusion with 2 % paraformaldehyde (PFA, Cat.# J61984 Alfa Aesar, Haverhill, United States). Tumors were dissected and fixed with 4 % PFA for 24-48 hours and frozen in the fume of liquid nitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The medium was then replaced by the normal cell culture medium supplemented with 2 mM L-methionine (Alfa Aesar J61904) for 0-3 hr at 37°C for the chase ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with 0.5 mg/ml of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Alfa Aesar, Haverhill, MA) containing media for 3.5 h ...
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bioRxiv - Cell Biology 2022Quote: ... that was threaded through a heated platinum coil (∼2 mm in diam.; Pt wire is 0.3 mm in diam., Alfa Aesar) upon application of a desired voltage ...
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bioRxiv - Cancer Biology 2023Quote: ... and 75 μL of a pre-filtered solution of 1 mg/mL XTT sodium salt (Alfa Aesar, 111072-31-2) and 20 μL/mL XTT activator (Trevigen ...
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bioRxiv - Cancer Biology 2023Quote: ... and 75 µL of a pre-filtered solution of 1 mg/mL XTT sodium salt (Alfa Aesar, 111072-31-2) and 20 µL/mL XTT activator (Trevigen ...
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bioRxiv - Biochemistry 2019Quote: ... UK) for 2 mins at 37°C prior to the addition of calcium chloride (25 μl, 50 mM; Alfa Aesar, UK). The time taken for clot formations to occur (an upper maximal of 2 mins was imposed ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were grown to confluency and then screened based on functional testing of the XBP1s construct using RT-PCR (described below) with or without 2 μg/mL doxycycline (dox; Alfa Aesar). The selected SupT1 single colony cell line encoding tetracycline-inducible XBP1s was then transduced with lentivirus encoding DHFR.ATF6(1–373 ...
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bioRxiv - Biochemistry 2022Quote: ... FDH2 was assayed in an argon (Airgas AR-300) atmosphere with 2 mM or 20 mM Benzyl viologen (Alfa Aesar H66836) in 50 mM Tris pH 8.0 with 20 mM glucose and supplemented with glucose oxidase (Sigma-G7141-50ku ...
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bioRxiv - Genomics 2019Quote: ... 10M cells were treated with 4Gy IR (RS-2000, RadSource) and 2 hours later were fixed by addition of 1% paraformeldehyde (Alfa Aesar) at room temperature for 10 minutes with agitation ...
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bioRxiv - Biophysics 2019Quote: ... a 2 mL centrifuge tube was charged with 1 mg of dried EB-NS and 0.5 mL of glycerol (Alfa Aesar, 99+%). The powder was re-dispersed in glycerol by 2 min of tip sonication in an ice bath at 60 W power ...
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bioRxiv - Bioengineering 2022Quote: ... TLS sample was prepared in 250 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP HCl, Cat# H51864, Alfa Aesar, Haverhill, MA, USA) in PBS.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The transfection mixture was prepared in Opti-MEM media with a ratio of 2 μL of PEI (Alfa Aesar, 1mg/mL) per 1 μg of DNA ...
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bioRxiv - Systems Biology 2022Quote: ... The cells were transiently transfected with N-terminal YFP-fused NF2 variants (pdEYFP) and mutant proteins using polyethylenimine (PEI, 1 mg/ml, Alfa Aesar, 9002-98-6) at 1:5 ratio of pDNA:transfection reagent ...
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bioRxiv - Biophysics 2022Quote: ... The transient mammalian transfection was applied in a mass ratio of 1:2 πα-SHP-H-Sgene : PEI (linear, MW > 25000, Alfa Aesar) (30) ...
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bioRxiv - Microbiology 2021Quote: ... Culture medium was supplemented with 20 µg/mL of the quinone 1,4-dihydroxy-2-naphthoic acid (DHNA) (Alfa Aesar, Haverhill, MA, USA), 1.25 mM ferric ammonium citrate (VWR ...
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bioRxiv - Cancer Biology 2021Quote: ... each consisting of 5 days of DSS in drinking water (2%, 40 kDa) followed by 7 days of recovery (Alfa Aesar #J63606).
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bioRxiv - Microbiology 2022Quote: ... 1μg gRNA and 3μg of pCTR4-2-FKS1 promoter construct (48) and cells were plated on YPD with 400μg/mL hygromycin B (Alfa Aesar, Cat# J69681). Transformants were verified with PCR spanning the joint between the promoter construct and FKS1 coding sequence and tested for response to copper by growth assay and gene expression.
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bioRxiv - Molecular Biology 2024Quote: ... 2 × 105 cells were seeded onto glass coverslip (SLS, MIC3300) overnight before fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899) for 10min ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was composed of equal amounts of protein in addition to varying amounts of sodium dodecyl sulfate buffer (SDS sample buffer; Alfa Aesar, Ward Hill, MA, USA) supplemented with beta-mercapto-ethanol and water ...
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bioRxiv - Neuroscience 2022Quote: ... were pulled to a shank length of approximately 0.6 cm (5-8 MΩ) using a Narishige PC-10 puller (Tokyo, Japan) and filled with 2% Pontamine sky blue dye (Alfa Aesar, Haverhill, MA) dissolved in sodium acetate (0.5 M ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Microbiology 2022Quote: ... Biotin was conjugated to the probes in a click reaction with 0.4 mg cleared lysate in 180 μL PBS and 20 μL 10X click master mix (1 mM azido-PEG3-biotin [Alfa Aesar], 10 mM tris(2-carboxyethyl)phosphine hydrochloride ...