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Citations for Alfa Aesar :
301 - 350 of 363 citations for 7 Chloro 9 methyl 3 4 dihydro 2H benzo b oxepin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The infection was terminated by washing the cells with PBS and fixing them with 4% paraformaldehyde (Alfa Aesar) for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diluted with 30 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
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bioRxiv - Genetics 2023Quote: ... At the designated timepoints the supernatant was discarded and replaced with 4% PFA (cat.no. 43368, Alfa Aesar, USA). The plates were incubated at 4°C for 24h ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diluted with 30 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 95 °C for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... Animals were grown (from embryo onward) on NGM plates supplemented with OP50 and 4mM auxin in EtOH (indole-3 acetic acid, IAA, Alfa Aesar) at 25°C to degrade DAF-16 panneuronally and to induce dauer formation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a subset of worms from each replicate were screened on NGM-agar plates containing 1 mM indole-3-acetic acid (Auxin, Alfa Aesar) following Kasimatis et al ...
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bioRxiv - Cell Biology 2023Quote: ... The degron-tagged L1 larvae were plated on NGM plates with 500 μM indole-3-acetic acid (IAA; Alfa Aesar, 10171307). Worms were placed on IAA plates and collected at young adult stage for further analysis.
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bioRxiv - Biochemistry 2019Quote: ... and then crocein scarlet-acid fuchsin solution for 2 minutes (4 parts crocein scarlet – 0.1% crocein scarlet [Alfa Aesar, J66876] and 0.5% glacial acetic acid ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed three times with 200μL of FACS buffer and fixed with 200μL of 4% PFA (Alfa Aesar) for 15 mins at room temperature ...
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bioRxiv - Neuroscience 2019Quote: 23 DIV neurons were fixed in 4 % (w/v) paraformaldehyde in phosphate-buffered saline (PBS) (Alfa Aesar, MA, USA) and non-specific fluorescence quenched by incubation in 50 mM NH4Cl in PBS for 10 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... the cancer cell lines MCF7 and MDA-MB-231 were fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Haverhill, MA) for 15-30 min at room temperature following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: Auxin treatment was performed as previously described(46) using the natural auxin indole-3-acetic acid (IAA) purchased from Alfa Aesar (#A10566). Auxin-containing plates were prepared by adding auxin in ethanol (1mM final concentration ...
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bioRxiv - Developmental Biology 2020Quote: Gravid JDW87 adults were allowed to lay eggs for 1 hour on MYOB with and without 4mM auxin (Indole 3-acetic acid, Alfa Aesar #AAA1055622), then removed ...
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bioRxiv - Molecular Biology 2020Quote: ... The degron tagged-L1 larval stage worms were plated on NGM plates with 100 μM auxin indole-3-acetic acid (IAA) (Alfa Aesar, A10556) unless otherwise stated ...
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bioRxiv - Molecular Biology 2020Quote: ... The degron tagged-L1 larval stage worms were plated on NGM plate containing 100 μM auxin indole-3-acetic acid (IAA) (Alfa Aesar, A10556). Worms were collected for gonad dissection when the L1 reached adult stages.
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bioRxiv - Cell Biology 2021Quote: ... Acute sterol depletion was achieved by 1 h incubation with 5 mM MβCD (Alfa Aesar, 1303.31 g/mol) at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... Lyophilized samples were dissolved in a buffer consisting of 5 mM MOPS sodium salt (Alfa Aesar, MA, USA) and water ...
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bioRxiv - Microbiology 2022Quote: ... the agar was supplemented with hemin (5 μg/mL) (MilliporeSigma) and vitamin K1 (1 μg/mL) (Alfa Aesar) and plates were poured ...
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bioRxiv - Microbiology 2023Quote: ... fragilis NCTC 9343 was grown in Brain heart infusion (BHIS) supplemented with 5 mg/L hemin (Alfa Aesar) in 1 N NaOH ...
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bioRxiv - Microbiology 2023Quote: ... fragilis NCTC 9343 was grown in Brain heart infusion (BHIS) supplemented with 5 mg/L hemin (Alfa Aesar) in 1 N NaOH ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were calibrated using a matrix-matched serial dilution of Specpure multi-element plasma standard solution 4 (Alfa Aesar) set by linear regression.
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bioRxiv - Plant Biology 2020Quote: ... All samples were calibrated using a matrix-matched serial dilution of Specpure multi-element plasma standard solution 4 (Alfa Aesar) set by linear regression.
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bioRxiv - Cell Biology 2021Quote: ... washed twice with phosphate buffered saline (PBS) at 37°C and fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar) at 37°C ...
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bioRxiv - Physiology 2022Quote: ... cells were washed twice for 5 min with PBS supplemented with 500 μM MgCl2 and 0.5 μM CaCl2 (PBS-S) then fixed with 4% paraformaldehyde (catalog no. 43368, Alfa Aesar) for 20 min and washed with PBS-S twice for 5 min ...
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bioRxiv - Immunology 2020Quote: ... BL21 cells were spun down (3000 RPM, 30 min, 4 °C) and resuspended in Tris-buffered saline (TBS, Alfa Aesar) containing cOmplete™ protease inhibitor cocktail (Sigma Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... Propidium iodide was used as a dead cell marker (1mg/ml aqueous solution; 25535-16-4, Alfa Aesar, Lancashire, UK). Cells were then sorted using fluorescence-activated cell sorting (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 100 μL PBS and fixed by pipetting into 24 μL fixation cocktail (4 μL 1M NaPO4 buffer, pH 7.4, and 20 μL 16% (wt/vol) paraformaldehyde (Alfa Aesar)) ...
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bioRxiv - Microbiology 2019Quote: ... the cell pellet obtained from initial centrifugation was incubated at 37°C for 1 hour with 300 rpm mixing in 250 μL 3 mg/mL lysozyme (Alfa Aesar, Haverhill, MA) in Tris-EDTA buffer (Sigma-Aldrich ...
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bioRxiv - Genetics 2019Quote: ... the 4 ml cultures were grown in the presence or absence of 0.5 mM of orsellinic acid (Alfa Aesar, Heysham, England) in DMSO.
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatants were collected by centrifugation at 3,200 x g at 4°C for 30 min and the pH was adjusted to the same value as fresh media 5N KOH (Alfa Aesar) with the Mettler Toledo InLab Micro pH electrode ...
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bioRxiv - Neuroscience 2020Quote: ... adult mice were killed rapidly by cervical dislocation and the brains dissected and immediately post-fixed in 10 ml 4% paraformaldehyde (Alfa Aesar) in 0.1 M phosphate buffer for 4-5 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... or 300,000 (MDA-MB-231) cells were plated per dish and cells were fixed 18 h later with 4% paraformaldehyde (Alfa Aesar) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... pups of both sexes were euthanized with an overdose (>200mg/kg) of 20% pentobarbital sodium solution and transcardially perfused with 4% paraformaldehyde (PFA, Alfa Aesar) in phosphate-buffered solution (PBS ...
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bioRxiv - Neuroscience 2019Quote: ... transcardiac perfusion of phosphate buffered saline (PBS; Oxoid) was performed and followed by transcardiac perfusion of 4% (v/v) paraformaldehyde (PFA; Alfa Aesar). PBS and PFA volumes used were ...
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bioRxiv - Cell Biology 2019Quote: ... mice were sacrificed following institutional guidelines and solidified Matrigel plugs were excised and fixed using 4% paraformaldehyde (Alfa Aesar, AA43368-9M). Plugs were cryosectioned ...
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bioRxiv - Biochemistry 2020Quote: ... Incubations were quenched by diluting the incubation with 20 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
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bioRxiv - Biophysics 2023Quote: The tissue was fixed at the end of each experiment with 4% paraformaldehyde in PBS (Alfa Aesar, ThermoFisher GmbH, Kandel, Germany) for 5 min at room temperature followed by thoroughly washing using 1x Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Microbiology 2023Quote: ... of each sample were spotted onto the surface of Mueller-Hinton agar (30 ml in a standard 100 mm petri dish) containing 4 µg/ml ticarcillin-clavulanate (15:1, Alfa Aesar). Plates were incubated at 37°C for approximately 24 h.
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bioRxiv - Cancer Biology 2023Quote: ... Protein pellet was dissolved in RIPA buffer and then 20 μL of 4× reducing Laemmli SDS sample loading buffer (Cat. No. J63615, Alfa Aesar) was added and heated at 95 °C for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred to a new bottle and stored at 4 °C until use. N,N’-methylenebis(acrylamide) (BIS; catalog no. J66710) was purchased from Alfa Aesar. Ammonium persulfate (APS ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then boiled for 8 min at 90 °C after addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar) and ran on precast 4−20% Criterion TGX gels (Bio-Rad).
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bioRxiv - Neuroscience 2019Quote: ... animals were mounted onto 5% agarose pads and immobilized using 0.5µl of 0.10 µmpolystyrene latex microsphere (Alfa Aesar # 427124Y). The coverslip was sealed with Vaseline (Vaseline® Jelly Original) ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were diluted to 0.5 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... The samples were diluted to 0.5 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... each sample was diluted to 2 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
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bioRxiv - Immunology 2023Quote: ... 2.5 µg of plasmid DNA was mixed with 5 µL Polyethyleneimine (PEI) at 1ug/ml stock solution (Alfa Aesar) and incubated for 15 min at room temperature before being added to the RD114 cells ...
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bioRxiv - Microbiology 2022Quote: ... This was followed by fixing the remaining adherent A549 cells in the wells with 4% formaldehyde in PBS for 10 min (Alfa Aesar, UK), permeabilizing the cells with 0.2% Triton-X100 (VWR ...
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bioRxiv - Neuroscience 2022Quote: ... tadpoles were washed three times with PBS (-/-) and fixed in 4% Paraformaldehyde in PBS + Mg + EGTA (MEMFA) (Alfa Aesar, Tewksbury, MA, USA) for 2 hours at RT ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... difficile as described above were washed thrice with PBS to remove unadhered bacteria and fixed with 4% paraformaldehyde (PFA) (Alfa Aesar, USA) for 15 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 × 105 cells were seeded onto glass coverslip (SLS, MIC3300) overnight before fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899) for 10min ...