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Citations for Alfa Aesar :
201 - 248 of 248 citations for 6 Methyl 3 5 heptadien 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The degron tagged-L1 larval stage worms were plated on NGM plate containing 100 μM auxin indole-3-acetic acid (IAA) (Alfa Aesar, A10556). Worms were collected for gonad dissection when the L1 reached adult stages.
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bioRxiv - Cell Biology 2021Quote: ... Acute sterol depletion was achieved by 1 h incubation with 5 mM MβCD (Alfa Aesar, 1303.31 g/mol) at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... Lyophilized samples were dissolved in a buffer consisting of 5 mM MOPS sodium salt (Alfa Aesar, MA, USA) and water ...
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bioRxiv - Microbiology 2022Quote: ... the agar was supplemented with hemin (5 μg/mL) (MilliporeSigma) and vitamin K1 (1 μg/mL) (Alfa Aesar) and plates were poured ...
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bioRxiv - Microbiology 2023Quote: ... fragilis NCTC 9343 was grown in Brain heart infusion (BHIS) supplemented with 5 mg/L hemin (Alfa Aesar) in 1 N NaOH ...
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bioRxiv - Microbiology 2023Quote: ... fragilis NCTC 9343 was grown in Brain heart infusion (BHIS) supplemented with 5 mg/L hemin (Alfa Aesar) in 1 N NaOH ...
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bioRxiv - Developmental Biology 2019Quote: ... clarified in 50% methanol/50% BABB (benzyl alcohol:benzyl benzoate 1:2 ratio, Alfa Aesar and Sigma), transferred into 100% BABB in glass capillaries and imaged on a Leica SP8 confocal microscope.
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bioRxiv - Cell Biology 2019Quote: ... infected cells were selected by incubation in growth medium containing puromycin (2 μg/ml; Alfa Aesar) for 1 week ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 4-(2-Hydroxyethyl)-1-piperazinepropane-sulfonic acid (EPPS) was purchased from Alfa Aesar (Product no.: J60511). Two different type of poly acrylic acids were used (PAA) ...
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bioRxiv - Microbiology 2019Quote: ... the cell pellet obtained from initial centrifugation was incubated at 37°C for 1 hour with 300 rpm mixing in 250 μL 3 mg/mL lysozyme (Alfa Aesar, Haverhill, MA) in Tris-EDTA buffer (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... animals were mounted onto 5% agarose pads and immobilized using 0.5µl of 0.10 µmpolystyrene latex microsphere (Alfa Aesar # 427124Y). The coverslip was sealed with Vaseline (Vaseline® Jelly Original) ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were diluted to 0.5 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... The samples were diluted to 0.5 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
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bioRxiv - Immunology 2023Quote: ... 2.5 µg of plasmid DNA was mixed with 5 µL Polyethyleneimine (PEI) at 1ug/ml stock solution (Alfa Aesar) and incubated for 15 min at room temperature before being added to the RD114 cells ...
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bioRxiv - Pathology 2022Quote: ... they were counterstained for 2 minutes with 1% methylene blue in distilled water (Alfa Aesar, Karlsruhe, Germany). A standard Hematoxylin and Eosin (H&E ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with isopropanol (60%) for 5 minutes and then with ORO solution (ORO, 2mg/mL, Alfa Aesar, A12989) for 20 min ...
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bioRxiv - Immunology 2021Quote: Viruses and control recombinant SARS-CoV-2 spike protein (LakePharma) were denatured with Laemmli sample buffer (Alfa Aesar) by heating at 95 °C for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and BAPTA (1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid) from Alfa Aesar (Tewksbury, MA). GSK101 (1 mM) ...
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bioRxiv - Microbiology 2022Quote: The synthesis began with a reaction of commercially available N-Boc-L-tyrosine (2 mmol, Alfa Aesar, 98%) and oleylamine (2.4 mmol ...
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bioRxiv - Immunology 2019Quote: Immunofluorescence on saponin-permeabilized cells was carried out as described previously [5] except that cells were fixed in methanol-free 4% (w/v) paraformaldehyde (PFA, Alfa Aesar) in phosphate buffered saline (PBS) ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked at RT with blocking buffer (5% skim milk powder in TBST containing sodium orthovanadate (Na3VO4, 1mM, Alfa Aesar) and sodium fluoride (NaF ...
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bioRxiv - Microbiology 2023Quote: ... cells were harvested by centrifugation (4720 x g for 5 min) and resuspended in filter-sterilised HEPES-buffered saline (HBS, Alfa Aesar). Diluted cell suspensions were added to tubes containing HBS ...
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bioRxiv - Biochemistry 2019Quote: ... and then crocein scarlet-acid fuchsin solution for 2 minutes (4 parts crocein scarlet – 0.1% crocein scarlet [Alfa Aesar, J66876] and 0.5% glacial acetic acid ...
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bioRxiv - Bioengineering 2019Quote: ... and then simultaneously blocked and permeabilized with 2% BSA in PBS++ with 0.1% saponin (Alfa Aesar, cat. no. A18820) for 1hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... mice were anesthetized and sacrificed by transcardial perfusion with 2 % paraformaldehyde (PFA, Cat.# J61984 Alfa Aesar, Haverhill, United States). Tumors were dissected and fixed with 4 % PFA for 24-48 hours and frozen in the fume of liquid nitrogen ...
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bioRxiv - Genomics 2021Quote: ... green bean size frozen tissue incubated in the ice-cold 800 µl of 1X homogenization unstable buffer(5 mM CaCl2 (Alfa Aesar, J63122), 3 mM Mg(Ac)2 (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... PCR tubes containing 7-day-old seedlings were transferred to a 3D-printed holder made to hold up to 5 seedlings and platinum-iridium foil electrodes (Platinum:Iridium = 80:20; Alfa Aesar 41805.FF), as previously described (Salvalaio et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The medium was then replaced by the normal cell culture medium supplemented with 2 mM L-methionine (Alfa Aesar J61904) for 0-3 hr at 37°C for the chase ...
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bioRxiv - Cell Biology 2022Quote: ... that was threaded through a heated platinum coil (∼2 mm in diam.; Pt wire is 0.3 mm in diam., Alfa Aesar) upon application of a desired voltage ...
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bioRxiv - Cancer Biology 2023Quote: ... and 75 μL of a pre-filtered solution of 1 mg/mL XTT sodium salt (Alfa Aesar, 111072-31-2) and 20 μL/mL XTT activator (Trevigen ...
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bioRxiv - Cancer Biology 2023Quote: ... and 75 µL of a pre-filtered solution of 1 mg/mL XTT sodium salt (Alfa Aesar, 111072-31-2) and 20 µL/mL XTT activator (Trevigen ...
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bioRxiv - Microbiology 2021Quote: ... Overnight cultures of donor strain were inoculated from cryopreserved stocks (−80°C in LB + 10% glycerol) into 5 mL LB amended with 10 μg/mL kanamycin and 150 μL of 100 mM DAP (Alfa Aesar, Haverhill, MA) and incubated shaking at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... all dishes were incubated at 37°C with 5% CO2 before being washed with sterile PBS and fixed with 4% paraformaldehyde (Alfa Aesar, Tewksbury, MA). Confocal laser scanning microscopy (CLSM ...
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bioRxiv - Microbiology 2019Quote: ... F1 mice at 7 weeks of age (7-11/treatment group) were treated for 5 consecutive days with 1.5% DSS (Alfa Aesar, Haverhill, MA, USA) in sterile drinking water ...
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bioRxiv - Biophysics 2022Quote: ... Cal-520-AM-loaded cells were removed from the CO2 incubator and equilibrated at room temperature for 5 minutes prior to IP3 stimulation by the addition of 200 µl of 1 mM carbachol (Alfa Aesar; #L06674-06), a Gαq-coupled M3 muscarinic receptor agonist ...
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bioRxiv - Biochemistry 2019Quote: ... UK) for 2 mins at 37°C prior to the addition of calcium chloride (25 μl, 50 mM; Alfa Aesar, UK). The time taken for clot formations to occur (an upper maximal of 2 mins was imposed ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were grown to confluency and then screened based on functional testing of the XBP1s construct using RT-PCR (described below) with or without 2 μg/mL doxycycline (dox; Alfa Aesar). The selected SupT1 single colony cell line encoding tetracycline-inducible XBP1s was then transduced with lentivirus encoding DHFR.ATF6(1–373 ...
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bioRxiv - Biochemistry 2022Quote: ... FDH2 was assayed in an argon (Airgas AR-300) atmosphere with 2 mM or 20 mM Benzyl viologen (Alfa Aesar H66836) in 50 mM Tris pH 8.0 with 20 mM glucose and supplemented with glucose oxidase (Sigma-G7141-50ku ...
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bioRxiv - Genomics 2019Quote: ... 10M cells were treated with 4Gy IR (RS-2000, RadSource) and 2 hours later were fixed by addition of 1% paraformeldehyde (Alfa Aesar) at room temperature for 10 minutes with agitation ...
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bioRxiv - Biophysics 2019Quote: ... a 2 mL centrifuge tube was charged with 1 mg of dried EB-NS and 0.5 mL of glycerol (Alfa Aesar, 99+%). The powder was re-dispersed in glycerol by 2 min of tip sonication in an ice bath at 60 W power ...
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bioRxiv - Bioengineering 2022Quote: ... TLS sample was prepared in 250 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP HCl, Cat# H51864, Alfa Aesar, Haverhill, MA, USA) in PBS.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The transfection mixture was prepared in Opti-MEM media with a ratio of 2 μL of PEI (Alfa Aesar, 1mg/mL) per 1 μg of DNA ...
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bioRxiv - Microbiology 2022Quote: MICs were determined by the agar dilution method on BHI agar either with or without 5 µg/ml of porcine hemin (Alfa Aesar, catalog no. A11165) and doubling dilutions of metronidazole (0.125-32 µg/ml ...
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bioRxiv - Biophysics 2022Quote: ... The transient mammalian transfection was applied in a mass ratio of 1:2 πα-SHP-H-Sgene : PEI (linear, MW > 25000, Alfa Aesar) (30) ...
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bioRxiv - Microbiology 2021Quote: ... Culture medium was supplemented with 20 µg/mL of the quinone 1,4-dihydroxy-2-naphthoic acid (DHNA) (Alfa Aesar, Haverhill, MA, USA), 1.25 mM ferric ammonium citrate (VWR ...
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bioRxiv - Microbiology 2022Quote: ... 1μg gRNA and 3μg of pCTR4-2-FKS1 promoter construct (48) and cells were plated on YPD with 400μg/mL hygromycin B (Alfa Aesar, Cat# J69681). Transformants were verified with PCR spanning the joint between the promoter construct and FKS1 coding sequence and tested for response to copper by growth assay and gene expression.
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bioRxiv - Molecular Biology 2024Quote: ... 2 × 105 cells were seeded onto glass coverslip (SLS, MIC3300) overnight before fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899) for 10min ...
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bioRxiv - Microbiology 2022Quote: ... Biotin was conjugated to the probes in a click reaction with 0.4 mg cleared lysate in 180 μL PBS and 20 μL 10X click master mix (1 mM azido-PEG3-biotin [Alfa Aesar], 10 mM tris(2-carboxyethyl)phosphine hydrochloride ...