Labshake search
Citations for Alfa Aesar :
301 - 350 of 468 citations for 6 4 methylpiperazin 1 yl pyridine 3 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2021Quote: ... washed with PBST (1X PBS, 0.1% Triton-X 100), blocked in PBTA (1X PBS, 0.1% Triton-X 100, 3% BSA) (Alfa Aesar), and then incubated in the appropriate primary antibody overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... tubing and pipette tips used to handle wounded cells were treated with 3% Pluronic F-68 (J6608736, Alfa Aesar) in deionized (DI ...
-
bioRxiv - Molecular Biology 2022Quote: ... Colitis was induced by replacing normal drinking water with 3% (w/v) DSS (molecular mass, 40 kDa Alfa Aesar) for 7 days ...
-
bioRxiv - Microbiology 2023Quote: ... The recovery broth (RB) used during transformation was commercial MRS supplemented with 3 mM L-cysteine (Alfa Aesar, A10389), 150 µM iron(II ...
-
bioRxiv - Genetics 2023Quote: ... with PBST (1X PBS, 0.1% Triton-X 100), blocked in PBTA (1X PBS, 0.1% Triton-X100, 3% BSA) (Alfa Aesar), and then incubated with primary antibodies overnight at 4°C ...
-
bioRxiv - Neuroscience 2019Quote: ... NIH3T3 cells were cultured for 4 hours in the presence of 100 nM Bafilomycin A (Alfa Aesar) to block autophagy and/or in DMEM media without glutamine and serum for autophagy induction prior to downstream analysis ...
-
bioRxiv - Cancer Biology 2019Quote: ... Samples were then diluted with 20 μL of 4 × reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
-
bioRxiv - Developmental Biology 2022Quote: Cells cultured in a µ-slide 8 well dish were fixed with 4% paraformaldehyde (J61899, Alfa Aesar) for 30 minutes at room temperature ...
-
Extracellular matrix micropatterning technology for whole cell cryogenic electron microscopy studiesbioRxiv - Bioengineering 2019Quote: ... They were fixed by incubation in a solution of 4% paraformaldehyde (AA433689M, Alfa Aesar, Haverhill, MA, USA) in PBS for 15 min at room temperature.
-
bioRxiv - Biochemistry 2023Quote: ... The reaction was stopped by the addition of 4 × reducing Laemmli SDS sample loading buffer (Alfa Aesar). After boiling at 95 °C for 5 min ...
-
bioRxiv - Biophysics 2023Quote: ... 3T3 fibroblast cells were fixed for 15 min in 4% paraformaldehyde (43368; Alfa Aesar, Haverhill, MA, USA) in phosphate-buffered saline (PBS) ...
-
bioRxiv - Cell Biology 2021Quote: ... Dishes were rinsed 3 times with phosphate buffered saline and then imaged in Tyrode’s Solution (Alfa Aesar, cat#:J67593-AP).
-
bioRxiv - Cancer Biology 2022Quote: ... Cas9 and BFP expression induction was achieved by treatment of the cells for 3 days with doxycycline (Dox) (1µg/ml) (Hyclate, Alfa Aesar) and single cell clones were seeded by sorting BFP positive cells using the BD FACSAria™ Cell Sorter system ...
-
bioRxiv - Immunology 2021Quote: ... Cells were fixed for 30 minutes at room temperature in 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899). Cells were washed in PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were fixed with 4% paraformaldehyde (PFA; Electron Microscopy Services) with 0.075 mg/mL saponin (Alfa Aesar,Sigma) diluted in phosphate-buffered saline (PBS ...
-
bioRxiv - Biophysics 2021Quote: ... 3T3 fibroblast and HUVEC cells were fixed for 15 min in 4% paraformaldehyde (43368; Alfa Aesar, Haverhill, MA) in phosphate-buffered saline (PBS) ...
-
bioRxiv - Genomics 2022Quote: ... The infection was terminated by washing the cells with PBS and fixing them with 4% paraformaldehyde (Alfa Aesar) for 15 min ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were then diluted with 30 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
-
bioRxiv - Genetics 2023Quote: ... At the designated timepoints the supernatant was discarded and replaced with 4% PFA (cat.no. 43368, Alfa Aesar, USA). The plates were incubated at 4°C for 24h ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were then diluted with 30 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 95 °C for 5 min ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were washed three times with 200μL of FACS buffer and fixed with 200μL of 4% PFA (Alfa Aesar) for 15 mins at room temperature ...
-
bioRxiv - Neuroscience 2019Quote: 23 DIV neurons were fixed in 4 % (w/v) paraformaldehyde in phosphate-buffered saline (PBS) (Alfa Aesar, MA, USA) and non-specific fluorescence quenched by incubation in 50 mM NH4Cl in PBS for 10 minutes ...
-
bioRxiv - Bioengineering 2023Quote: ... the cancer cell lines MCF7 and MDA-MB-231 were fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Haverhill, MA) for 15-30 min at room temperature following the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2020Quote: ... All samples were calibrated using a matrix-matched serial dilution of Specpure multi-element plasma standard solution 4 (Alfa Aesar) set by linear regression.
-
bioRxiv - Plant Biology 2020Quote: ... All samples were calibrated using a matrix-matched serial dilution of Specpure multi-element plasma standard solution 4 (Alfa Aesar) set by linear regression.
-
bioRxiv - Cell Biology 2021Quote: ... washed twice with phosphate buffered saline (PBS) at 37°C and fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar) at 37°C ...
-
bioRxiv - Physiology 2022Quote: ... cells were washed twice for 5 min with PBS supplemented with 500 μM MgCl2 and 0.5 μM CaCl2 (PBS-S) then fixed with 4% paraformaldehyde (catalog no. 43368, Alfa Aesar) for 20 min and washed with PBS-S twice for 5 min ...
-
bioRxiv - Immunology 2020Quote: ... BL21 cells were spun down (3000 RPM, 30 min, 4 °C) and resuspended in Tris-buffered saline (TBS, Alfa Aesar) containing cOmplete™ protease inhibitor cocktail (Sigma Millipore) ...
-
bioRxiv - Cell Biology 2023Quote: ... Propidium iodide was used as a dead cell marker (1mg/ml aqueous solution; 25535-16-4, Alfa Aesar, Lancashire, UK). Cells were then sorted using fluorescence-activated cell sorting (BD Biosciences ...
-
bioRxiv - Microbiology 2024Quote: ... resuspended in 100 μL PBS and fixed by pipetting into 24 μL fixation cocktail (4 μL 1M NaPO4 buffer, pH 7.4, and 20 μL 16% (wt/vol) paraformaldehyde (Alfa Aesar)) ...
-
bioRxiv - Cell Biology 2023Quote: ... samples were washed 3×5 minutes with PBS and then mounted in 15 μL of mounting media (80% Glycerol (Alfa Aesar, Cat#36646); 0.1 M Tris•HCl (Calbiochem ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The supernatants were collected by centrifugation at 3,200 x g at 4°C for 30 min and the pH was adjusted to the same value as fresh media 5N KOH (Alfa Aesar) with the Mettler Toledo InLab Micro pH electrode ...
-
bioRxiv - Neuroscience 2020Quote: ... adult mice were killed rapidly by cervical dislocation and the brains dissected and immediately post-fixed in 10 ml 4% paraformaldehyde (Alfa Aesar) in 0.1 M phosphate buffer for 4-5 h at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... or 300,000 (MDA-MB-231) cells were plated per dish and cells were fixed 18 h later with 4% paraformaldehyde (Alfa Aesar) for 10 min ...
-
bioRxiv - Neuroscience 2020Quote: ... pups of both sexes were euthanized with an overdose (>200mg/kg) of 20% pentobarbital sodium solution and transcardially perfused with 4% paraformaldehyde (PFA, Alfa Aesar) in phosphate-buffered solution (PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... transcardiac perfusion of phosphate buffered saline (PBS; Oxoid) was performed and followed by transcardiac perfusion of 4% (v/v) paraformaldehyde (PFA; Alfa Aesar). PBS and PFA volumes used were ...
-
bioRxiv - Cell Biology 2019Quote: ... mice were sacrificed following institutional guidelines and solidified Matrigel plugs were excised and fixed using 4% paraformaldehyde (Alfa Aesar, AA43368-9M). Plugs were cryosectioned ...
-
bioRxiv - Immunology 2019Quote: Immunofluorescence on saponin-permeabilized cells was carried out as described previously [5] except that cells were fixed in methanol-free 4% (w/v) paraformaldehyde (PFA, Alfa Aesar) in phosphate buffered saline (PBS) ...
-
bioRxiv - Biochemistry 2020Quote: ... Incubations were quenched by diluting the incubation with 20 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein pellet was dissolved in RIPA buffer and then 20 μL of 4× reducing Laemmli SDS sample loading buffer (Cat. No. J63615, Alfa Aesar) was added and heated at 95 °C for 5 min ...
-
bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred to a new bottle and stored at 4 °C until use. N,N’-methylenebis(acrylamide) (BIS; catalog no. J66710) was purchased from Alfa Aesar. Ammonium persulfate (APS ...
-
bioRxiv - Biophysics 2023Quote: The tissue was fixed at the end of each experiment with 4% paraformaldehyde in PBS (Alfa Aesar, ThermoFisher GmbH, Kandel, Germany) for 5 min at room temperature followed by thoroughly washing using 1x Dulbecco’s Phosphate Buffered Saline (DPBS ...
-
Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... an overnight culture was grown in 5 ml LB (E. coli MG1655 recipient) or 5 ml LB (E & O Laboratories Ltd) + 4 µg/ml cefotaxime (Alfa Aesar) (donors ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were then boiled for 8 min at 90 °C after addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar) and ran on precast 4−20% Criterion TGX gels (Bio-Rad).
-
bioRxiv - Neuroscience 2020Quote: ... 1 μl Cas9 and 1 μl Fast Green (Alfa Aesar, Heysham, UK), was injected into the yolk of single cell stage embryos.
-
bioRxiv - Microbiology 2022Quote: ... This was followed by fixing the remaining adherent A549 cells in the wells with 4% formaldehyde in PBS for 10 min (Alfa Aesar, UK), permeabilizing the cells with 0.2% Triton-X100 (VWR ...
-
bioRxiv - Neuroscience 2022Quote: ... tadpoles were washed three times with PBS (-/-) and fixed in 4% Paraformaldehyde in PBS + Mg + EGTA (MEMFA) (Alfa Aesar, Tewksbury, MA, USA) for 2 hours at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... Slides were initially fixed in fresh 4% PFA for one hour at room temperature and washed with 1X PBS (Alfa Aesar, J62036) twice for two minutes each ...
-
Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... difficile as described above were washed thrice with PBS to remove unadhered bacteria and fixed with 4% paraformaldehyde (PFA) (Alfa Aesar, USA) for 15 min at RT ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 × 105 cells were seeded onto glass coverslip (SLS, MIC3300) overnight before fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899) for 10min ...