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Citations for Alfa Aesar :
201 - 228 of 228 citations for 5 TERT BUTOXYCARBONYLAMINO 2 CHLORO NICOTINIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... mice were anesthetized and sacrificed by transcardial perfusion with 2 % paraformaldehyde (PFA, Cat.# J61984 Alfa Aesar, Haverhill, United States). Tumors were dissected and fixed with 4 % PFA for 24-48 hours and frozen in the fume of liquid nitrogen ...
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bioRxiv - Genomics 2021Quote: ... green bean size frozen tissue incubated in the ice-cold 800 µl of 1X homogenization unstable buffer(5 mM CaCl2 (Alfa Aesar, J63122), 3 mM Mg(Ac)2 (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... PCR tubes containing 7-day-old seedlings were transferred to a 3D-printed holder made to hold up to 5 seedlings and platinum-iridium foil electrodes (Platinum:Iridium = 80:20; Alfa Aesar 41805.FF), as previously described (Salvalaio et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The medium was then replaced by the normal cell culture medium supplemented with 2 mM L-methionine (Alfa Aesar J61904) for 0-3 hr at 37°C for the chase ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with 0.5 mg/ml of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Alfa Aesar, Haverhill, MA) containing media for 3.5 h ...
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bioRxiv - Cell Biology 2022Quote: ... that was threaded through a heated platinum coil (∼2 mm in diam.; Pt wire is 0.3 mm in diam., Alfa Aesar) upon application of a desired voltage ...
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bioRxiv - Cancer Biology 2023Quote: ... and 75 μL of a pre-filtered solution of 1 mg/mL XTT sodium salt (Alfa Aesar, 111072-31-2) and 20 μL/mL XTT activator (Trevigen ...
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bioRxiv - Cancer Biology 2023Quote: ... and 75 µL of a pre-filtered solution of 1 mg/mL XTT sodium salt (Alfa Aesar, 111072-31-2) and 20 µL/mL XTT activator (Trevigen ...
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bioRxiv - Microbiology 2021Quote: ... Overnight cultures of donor strain were inoculated from cryopreserved stocks (−80°C in LB + 10% glycerol) into 5 mL LB amended with 10 μg/mL kanamycin and 150 μL of 100 mM DAP (Alfa Aesar, Haverhill, MA) and incubated shaking at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... all dishes were incubated at 37°C with 5% CO2 before being washed with sterile PBS and fixed with 4% paraformaldehyde (Alfa Aesar, Tewksbury, MA). Confocal laser scanning microscopy (CLSM ...
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bioRxiv - Microbiology 2019Quote: ... F1 mice at 7 weeks of age (7-11/treatment group) were treated for 5 consecutive days with 1.5% DSS (Alfa Aesar, Haverhill, MA, USA) in sterile drinking water ...
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bioRxiv - Biophysics 2022Quote: ... Cal-520-AM-loaded cells were removed from the CO2 incubator and equilibrated at room temperature for 5 minutes prior to IP3 stimulation by the addition of 200 µl of 1 mM carbachol (Alfa Aesar; #L06674-06), a Gαq-coupled M3 muscarinic receptor agonist ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed 3×5 minutes with PBS and then mounted in 15 μL of mounting media (80% Glycerol (Alfa Aesar, Cat#36646); 0.1 M Tris•HCl (Calbiochem ...
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bioRxiv - Biochemistry 2019Quote: ... UK) for 2 mins at 37°C prior to the addition of calcium chloride (25 μl, 50 mM; Alfa Aesar, UK). The time taken for clot formations to occur (an upper maximal of 2 mins was imposed ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were grown to confluency and then screened based on functional testing of the XBP1s construct using RT-PCR (described below) with or without 2 μg/mL doxycycline (dox; Alfa Aesar). The selected SupT1 single colony cell line encoding tetracycline-inducible XBP1s was then transduced with lentivirus encoding DHFR.ATF6(1–373 ...
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bioRxiv - Biochemistry 2022Quote: ... FDH2 was assayed in an argon (Airgas AR-300) atmosphere with 2 mM or 20 mM Benzyl viologen (Alfa Aesar H66836) in 50 mM Tris pH 8.0 with 20 mM glucose and supplemented with glucose oxidase (Sigma-G7141-50ku ...
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bioRxiv - Genomics 2019Quote: ... 10M cells were treated with 4Gy IR (RS-2000, RadSource) and 2 hours later were fixed by addition of 1% paraformeldehyde (Alfa Aesar) at room temperature for 10 minutes with agitation ...
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bioRxiv - Biophysics 2019Quote: ... a 2 mL centrifuge tube was charged with 1 mg of dried EB-NS and 0.5 mL of glycerol (Alfa Aesar, 99+%). The powder was re-dispersed in glycerol by 2 min of tip sonication in an ice bath at 60 W power ...
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bioRxiv - Bioengineering 2022Quote: ... TLS sample was prepared in 250 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP HCl, Cat# H51864, Alfa Aesar, Haverhill, MA, USA) in PBS.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The transfection mixture was prepared in Opti-MEM media with a ratio of 2 μL of PEI (Alfa Aesar, 1mg/mL) per 1 μg of DNA ...
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bioRxiv - Microbiology 2022Quote: MICs were determined by the agar dilution method on BHI agar either with or without 5 µg/ml of porcine hemin (Alfa Aesar, catalog no. A11165) and doubling dilutions of metronidazole (0.125-32 µg/ml ...
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bioRxiv - Biophysics 2023Quote: ... cells and beads were suspended into a 0.6 w/w% solution of methyl cellulose (MC) (4000 cPs, Alfa Aesar 036718.22, CAS 9004-67-5) dissolved in phosphate buffered saline (PBS ...
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bioRxiv - Biophysics 2022Quote: ... The transient mammalian transfection was applied in a mass ratio of 1:2 πα-SHP-H-Sgene : PEI (linear, MW > 25000, Alfa Aesar) (30) ...
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bioRxiv - Microbiology 2022Quote: ... 1μg gRNA and 3μg of pCTR4-2-FKS1 promoter construct (48) and cells were plated on YPD with 400μg/mL hygromycin B (Alfa Aesar, Cat# J69681). Transformants were verified with PCR spanning the joint between the promoter construct and FKS1 coding sequence and tested for response to copper by growth assay and gene expression.
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bioRxiv - Molecular Biology 2024Quote: ... 2 × 105 cells were seeded onto glass coverslip (SLS, MIC3300) overnight before fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899) for 10min ...
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bioRxiv - Biophysics 2022Quote: ... citrate blood was resuspended in a viscosity-adjusted measurement buffer (phosphate saline buffer without Mg2+ and Ca2+ (PBS−) containing 0.5% (w/v) methyl cellulose (4000 cPs, Alfa Aesar 036718.22, CAS# 9004-67-5); adjusted in HAAKE Falling Ball Viscometer type C (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Microbiology 2022Quote: ... Biotin was conjugated to the probes in a click reaction with 0.4 mg cleared lysate in 180 μL PBS and 20 μL 10X click master mix (1 mM azido-PEG3-biotin [Alfa Aesar], 10 mM tris(2-carboxyethyl)phosphine hydrochloride ...