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Citations for Alfa Aesar :
351 - 395 of 395 citations for 6 FLUORO 2 METHYL 3 4 PIPERIDINE 1H INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... adult mice were killed rapidly by cervical dislocation and the brains dissected and immediately post-fixed in 10 ml 4% paraformaldehyde (Alfa Aesar) in 0.1 M phosphate buffer for 4-5 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... or 300,000 (MDA-MB-231) cells were plated per dish and cells were fixed 18 h later with 4% paraformaldehyde (Alfa Aesar) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... pups of both sexes were euthanized with an overdose (>200mg/kg) of 20% pentobarbital sodium solution and transcardially perfused with 4% paraformaldehyde (PFA, Alfa Aesar) in phosphate-buffered solution (PBS ...
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bioRxiv - Neuroscience 2019Quote: ... transcardiac perfusion of phosphate buffered saline (PBS; Oxoid) was performed and followed by transcardiac perfusion of 4% (v/v) paraformaldehyde (PFA; Alfa Aesar). PBS and PFA volumes used were ...
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bioRxiv - Cell Biology 2019Quote: ... mice were sacrificed following institutional guidelines and solidified Matrigel plugs were excised and fixed using 4% paraformaldehyde (Alfa Aesar, AA43368-9M). Plugs were cryosectioned ...
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bioRxiv - Immunology 2019Quote: Immunofluorescence on saponin-permeabilized cells was carried out as described previously [5] except that cells were fixed in methanol-free 4% (w/v) paraformaldehyde (PFA, Alfa Aesar) in phosphate buffered saline (PBS) ...
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bioRxiv - Biochemistry 2020Quote: ... Incubations were quenched by diluting the incubation with 20 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
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bioRxiv - Biophysics 2023Quote: The tissue was fixed at the end of each experiment with 4% paraformaldehyde in PBS (Alfa Aesar, ThermoFisher GmbH, Kandel, Germany) for 5 min at room temperature followed by thoroughly washing using 1x Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Microbiology 2023Quote: ... of each sample were spotted onto the surface of Mueller-Hinton agar (30 ml in a standard 100 mm petri dish) containing 4 µg/ml ticarcillin-clavulanate (15:1, Alfa Aesar). Plates were incubated at 37°C for approximately 24 h.
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bioRxiv - Cancer Biology 2023Quote: ... Protein pellet was dissolved in RIPA buffer and then 20 μL of 4× reducing Laemmli SDS sample loading buffer (Cat. No. J63615, Alfa Aesar) was added and heated at 95 °C for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred to a new bottle and stored at 4 °C until use. N,N’-methylenebis(acrylamide) (BIS; catalog no. J66710) was purchased from Alfa Aesar. Ammonium persulfate (APS ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... an overnight culture was grown in 5 ml LB (E. coli MG1655 recipient) or 5 ml LB (E & O Laboratories Ltd) + 4 µg/ml cefotaxime (Alfa Aesar) (donors ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then boiled for 8 min at 90 °C after addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar) and ran on precast 4−20% Criterion TGX gels (Bio-Rad).
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bioRxiv - Pathology 2022Quote: ... they were counterstained for 2 minutes with 1% methylene blue in distilled water (Alfa Aesar, Karlsruhe, Germany). A standard Hematoxylin and Eosin (H&E ...
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bioRxiv - Microbiology 2022Quote: ... This was followed by fixing the remaining adherent A549 cells in the wells with 4% formaldehyde in PBS for 10 min (Alfa Aesar, UK), permeabilizing the cells with 0.2% Triton-X100 (VWR ...
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bioRxiv - Neuroscience 2022Quote: ... tadpoles were washed three times with PBS (-/-) and fixed in 4% Paraformaldehyde in PBS + Mg + EGTA (MEMFA) (Alfa Aesar, Tewksbury, MA, USA) for 2 hours at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were initially fixed in fresh 4% PFA for one hour at room temperature and washed with 1X PBS (Alfa Aesar, J62036) twice for two minutes each ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... difficile as described above were washed thrice with PBS to remove unadhered bacteria and fixed with 4% paraformaldehyde (PFA) (Alfa Aesar, USA) for 15 min at RT ...
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bioRxiv - Immunology 2021Quote: Viruses and control recombinant SARS-CoV-2 spike protein (LakePharma) were denatured with Laemmli sample buffer (Alfa Aesar) by heating at 95 °C for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and BAPTA (1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid) from Alfa Aesar (Tewksbury, MA). GSK101 (1 mM) ...
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bioRxiv - Microbiology 2022Quote: The synthesis began with a reaction of commercially available N-Boc-L-tyrosine (2 mmol, Alfa Aesar, 98%) and oleylamine (2.4 mmol ...
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bioRxiv - Cancer Biology 2021Quote: ... S34F and Q157R HEL cells and not-infected HEL cells were labeled in duplicate with 100 μM of the uridine analog 4-thiouridine (s4U, Alfa Aesar, Cat #AAJ60679MC) for 2 hours at 37°C in the dark ...
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bioRxiv - Microbiology 2021Quote: ... all dishes were incubated at 37°C with 5% CO2 before being washed with sterile PBS and fixed with 4% paraformaldehyde (Alfa Aesar, Tewksbury, MA). Confocal laser scanning microscopy (CLSM ...
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bioRxiv - Neuroscience 2022Quote: ... were dissected in ice-cold Ca2+-free saline solution and fixed for 24 h in 4% paraformaldehyde (PFA; J19943, Alfa Aesar; in PBS) at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... and then simultaneously blocked and permeabilized with 2% BSA in PBS++ with 0.1% saponin (Alfa Aesar, cat. no. A18820) for 1hr at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... each sample was diluted to 2 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
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bioRxiv - Cell Biology 2022Quote: ... mice were anesthetized and sacrificed by transcardial perfusion with 2 % paraformaldehyde (PFA, Cat.# J61984 Alfa Aesar, Haverhill, United States). Tumors were dissected and fixed with 4 % PFA for 24-48 hours and frozen in the fume of liquid nitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The medium was then replaced by the normal cell culture medium supplemented with 2 mM L-methionine (Alfa Aesar J61904) for 0-3 hr at 37°C for the chase ...
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bioRxiv - Developmental Biology 2019Quote: ... Next each sample was diluted to 2 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
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bioRxiv - Cell Biology 2022Quote: ... that was threaded through a heated platinum coil (∼2 mm in diam.; Pt wire is 0.3 mm in diam., Alfa Aesar) upon application of a desired voltage ...
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bioRxiv - Cancer Biology 2023Quote: ... and 75 μL of a pre-filtered solution of 1 mg/mL XTT sodium salt (Alfa Aesar, 111072-31-2) and 20 μL/mL XTT activator (Trevigen ...
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bioRxiv - Cancer Biology 2023Quote: ... and 75 µL of a pre-filtered solution of 1 mg/mL XTT sodium salt (Alfa Aesar, 111072-31-2) and 20 µL/mL XTT activator (Trevigen ...
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bioRxiv - Biochemistry 2019Quote: ... UK) for 2 mins at 37°C prior to the addition of calcium chloride (25 μl, 50 mM; Alfa Aesar, UK). The time taken for clot formations to occur (an upper maximal of 2 mins was imposed ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were grown to confluency and then screened based on functional testing of the XBP1s construct using RT-PCR (described below) with or without 2 μg/mL doxycycline (dox; Alfa Aesar). The selected SupT1 single colony cell line encoding tetracycline-inducible XBP1s was then transduced with lentivirus encoding DHFR.ATF6(1–373 ...
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bioRxiv - Biochemistry 2022Quote: ... FDH2 was assayed in an argon (Airgas AR-300) atmosphere with 2 mM or 20 mM Benzyl viologen (Alfa Aesar H66836) in 50 mM Tris pH 8.0 with 20 mM glucose and supplemented with glucose oxidase (Sigma-G7141-50ku ...
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bioRxiv - Genomics 2019Quote: ... 10M cells were treated with 4Gy IR (RS-2000, RadSource) and 2 hours later were fixed by addition of 1% paraformeldehyde (Alfa Aesar) at room temperature for 10 minutes with agitation ...
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bioRxiv - Biophysics 2019Quote: ... a 2 mL centrifuge tube was charged with 1 mg of dried EB-NS and 0.5 mL of glycerol (Alfa Aesar, 99+%). The powder was re-dispersed in glycerol by 2 min of tip sonication in an ice bath at 60 W power ...
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bioRxiv - Bioengineering 2022Quote: ... TLS sample was prepared in 250 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP HCl, Cat# H51864, Alfa Aesar, Haverhill, MA, USA) in PBS.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The transfection mixture was prepared in Opti-MEM media with a ratio of 2 μL of PEI (Alfa Aesar, 1mg/mL) per 1 μg of DNA ...
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bioRxiv - Biophysics 2022Quote: ... The transient mammalian transfection was applied in a mass ratio of 1:2 πα-SHP-H-Sgene : PEI (linear, MW > 25000, Alfa Aesar) (30) ...
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bioRxiv - Microbiology 2021Quote: ... Culture medium was supplemented with 20 µg/mL of the quinone 1,4-dihydroxy-2-naphthoic acid (DHNA) (Alfa Aesar, Haverhill, MA, USA), 1.25 mM ferric ammonium citrate (VWR ...
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bioRxiv - Cancer Biology 2021Quote: ... each consisting of 5 days of DSS in drinking water (2%, 40 kDa) followed by 7 days of recovery (Alfa Aesar #J63606).
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bioRxiv - Microbiology 2022Quote: ... 1μg gRNA and 3μg of pCTR4-2-FKS1 promoter construct (48) and cells were plated on YPD with 400μg/mL hygromycin B (Alfa Aesar, Cat# J69681). Transformants were verified with PCR spanning the joint between the promoter construct and FKS1 coding sequence and tested for response to copper by growth assay and gene expression.
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bioRxiv - Neuroscience 2022Quote: ... were pulled to a shank length of approximately 0.6 cm (5-8 MΩ) using a Narishige PC-10 puller (Tokyo, Japan) and filled with 2% Pontamine sky blue dye (Alfa Aesar, Haverhill, MA) dissolved in sodium acetate (0.5 M ...
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bioRxiv - Microbiology 2022Quote: ... Biotin was conjugated to the probes in a click reaction with 0.4 mg cleared lysate in 180 μL PBS and 20 μL 10X click master mix (1 mM azido-PEG3-biotin [Alfa Aesar], 10 mM tris(2-carboxyethyl)phosphine hydrochloride ...