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Citations for Alfa Aesar :
301 - 350 of 350 citations for 4 ArmPEG MA 10K since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... MTT (3-[4, 5- dimethyl thiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (Alfa Aesar Chemicals Co. Ltd. Shanghai, China), fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed three times with 200μL of FACS buffer and fixed with 200μL of 4% PFA (Alfa Aesar) for 15 mins at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... the effect of viscosity on synovial fluid-induced aggregation was assessed by imaging 1-hour after incubation in 10% BSF supplemented with increasing concentrations of high molecular weight hyaluronic acid sodium salt (1.5-1.8MDa) (Alfa Aesar, Haverhill, MA, USA) in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Culture medium was supplemented with 20 µg/mL of the quinone 1,4-dihydroxy-2-naphthoic acid (DHNA) (Alfa Aesar, Haverhill, MA, USA), 1.25 mM ferric ammonium citrate (VWR ...
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bioRxiv - Biophysics 2021Quote: ... We then detergent extracted the cells by resuspending the cell pellet in PEME buffer with 1% IGEPAL (CA-630, Alfa Aesar, MA) and incubating on ice for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... sections were placed into a plastic container with pH 6.0 citrate buffer: 0.294% Tri-sodium citrate (dihydrate) (Cat# A12274, Alfa Aesar, Tewksbury, MA, USA) + 0.05% TWEEN® 20 and heated in a food steamer (Hamilton Beach ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and positive clones were grown and selected in Luria broth media supplemented with 100ug/ml of Ampicillin (J60977, Alfa Aesar, Haverfill, MA). The NucleoSpin 96 Plasmid kit (740625.4 ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were calibrated using a matrix-matched serial dilution of Specpure multi-element plasma standard solution 4 (Alfa Aesar) set by linear regression.
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bioRxiv - Plant Biology 2020Quote: ... All samples were calibrated using a matrix-matched serial dilution of Specpure multi-element plasma standard solution 4 (Alfa Aesar) set by linear regression.
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bioRxiv - Cell Biology 2021Quote: ... washed twice with phosphate buffered saline (PBS) at 37°C and fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar) at 37°C ...
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bioRxiv - Physiology 2022Quote: ... cells were washed twice for 5 min with PBS supplemented with 500 μM MgCl2 and 0.5 μM CaCl2 (PBS-S) then fixed with 4% paraformaldehyde (catalog no. 43368, Alfa Aesar) for 20 min and washed with PBS-S twice for 5 min ...
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bioRxiv - Immunology 2020Quote: ... BL21 cells were spun down (3000 RPM, 30 min, 4 °C) and resuspended in Tris-buffered saline (TBS, Alfa Aesar) containing cOmplete™ protease inhibitor cocktail (Sigma Millipore) ...
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bioRxiv - Biophysics 2020Quote: ... Young adult worms were placed on NG agar plates containing 4 mM auxin (Indole-3 acetic acid, Alfa Aesar, # A10556) seeded with E ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 100 μL PBS and fixed by pipetting into 24 μL fixation cocktail (4 μL 1M NaPO4 buffer, pH 7.4, and 20 μL 16% (wt/vol) paraformaldehyde (Alfa Aesar)) ...
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bioRxiv - Cell Biology 2023Quote: ... Propidium iodide was used as a dead cell marker (1mg/ml aqueous solution; 25535-16-4, Alfa Aesar, Lancashire, UK). Cells were then sorted using fluorescence-activated cell sorting (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Overnight cultures of donor strain were inoculated from cryopreserved stocks (−80°C in LB + 10% glycerol) into 5 mL LB amended with 10 μg/mL kanamycin and 150 μL of 100 mM DAP (Alfa Aesar, Haverhill, MA) and incubated shaking at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: WT and Ern2-/- germ free mice received either regular drinking water or drinking water supplemented with 100 mM sodium butyrate (#A11079 Alfa Aesar, Tewksbury, MA) (47 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... perfluorotetradecanoic acid (PFTDA, CAS 376-06-7, purity ≥ 96%), and HFPO-DA (CAS 13252-13-6, purity ≥ 97%) were from Alfa Aesar (Havermill, MA). Perfluorohexanoic acid (PFHxA ...
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bioRxiv - Neuroscience 2022Quote: ... were pulled to a shank length of approximately 0.6 cm (5-8 MΩ) using a Narishige PC-10 puller (Tokyo, Japan) and filled with 2% Pontamine sky blue dye (Alfa Aesar, Haverhill, MA) dissolved in sodium acetate (0.5 M ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in complete RPMI 1640 media (without antibiotics) and incubated with 100 ng/mL phorbol 12-myristate 13-acetate (PMA, Alfa Aesar, Haverhill, MA). Cells were seeded at 50,000 cells/well into 96-well plates and incubated at 37°C for 3 days.
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bioRxiv - Neuroscience 2023Quote: ... were purchased from Sigma Aldrich (St Louis, MO, USA).(Dunham & Venton, 2020, 2022) meta-Chlorophenylpiperazine (m-CPP, 65369-76-8) was purchased from Alfa Aesar (Haverhill, MA, USA). FD&C Blue No ...
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bioRxiv - Cell Biology 2024Quote: ... and cells were washed twice with 300 µl of PBS for 5 minutes each and then permeabilized for 5 minutes with PBS/ 0.1% Triton X-100 (# J66624, Alfa Aesar, Ward Hill, MA). Cells were then washed three times for 5 minutes each with PBS and then stained with a 1:3,000 dilution of Phalloidin-555 Alexafluor (# ab176756 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PFOA (CAS 335-67-1, purity ≥ 95%), and HFPO-DA/GenX (CAS 1352-13-6, purity ≥ 97%) were from Alfa Aesar (Havermill, MA). Perfluorohexanoic acid (PFHxA ...
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bioRxiv - Biochemistry 2024Quote: ... CAT A12403.36) and sodium metaborate tetrahydrate (NaBO2·4H2O, CAS 10555-76-7, CAT A11803.36) were purchased from Alfa Aesar (Ward Hill, MA, USA). Purified water was obtained from a water PRO PS polisher (Labconco ...
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bioRxiv - Cancer Biology 2024Quote: ... Each sample comprised of equal amounts of protein and varying amounts of SDS buffer (SDS sample buffer; Alfa Aesar; Ward Hill, MA, USA) with M-PER ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatants were collected by centrifugation at 3,200 x g at 4°C for 30 min and the pH was adjusted to the same value as fresh media 5N KOH (Alfa Aesar) with the Mettler Toledo InLab Micro pH electrode ...
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bioRxiv - Neuroscience 2020Quote: ... adult mice were killed rapidly by cervical dislocation and the brains dissected and immediately post-fixed in 10 ml 4% paraformaldehyde (Alfa Aesar) in 0.1 M phosphate buffer for 4-5 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... or 300,000 (MDA-MB-231) cells were plated per dish and cells were fixed 18 h later with 4% paraformaldehyde (Alfa Aesar) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... pups of both sexes were euthanized with an overdose (>200mg/kg) of 20% pentobarbital sodium solution and transcardially perfused with 4% paraformaldehyde (PFA, Alfa Aesar) in phosphate-buffered solution (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Incubations were quenched by diluting the incubation with 20 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
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bioRxiv - Genetics 2020Quote: ... and auxin treatment was performed by transferring worms to bacteria-seeded plates containing 4 mM natural auxin (indole-3-acetic acid; Alfa Aesar). Worms were kept on these plates for 4 h (germline depletion ...
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bioRxiv - Microbiology 2023Quote: ... of each sample were spotted onto the surface of Mueller-Hinton agar (30 ml in a standard 100 mm petri dish) containing 4 µg/ml ticarcillin-clavulanate (15:1, Alfa Aesar). Plates were incubated at 37°C for approximately 24 h.
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bioRxiv - Cancer Biology 2023Quote: ... Protein pellet was dissolved in RIPA buffer and then 20 μL of 4× reducing Laemmli SDS sample loading buffer (Cat. No. J63615, Alfa Aesar) was added and heated at 95 °C for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred to a new bottle and stored at 4 °C until use. N,N’-methylenebis(acrylamide) (BIS; catalog no. J66710) was purchased from Alfa Aesar. Ammonium persulfate (APS ...
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bioRxiv - Biophysics 2023Quote: The tissue was fixed at the end of each experiment with 4% paraformaldehyde in PBS (Alfa Aesar, ThermoFisher GmbH, Kandel, Germany) for 5 min at room temperature followed by thoroughly washing using 1x Dulbecco’s Phosphate Buffered Saline (DPBS ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... an overnight culture was grown in 5 ml LB (E. coli MG1655 recipient) or 5 ml LB (E & O Laboratories Ltd) + 4 µg/ml cefotaxime (Alfa Aesar) (donors ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then boiled for 8 min at 90 °C after addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar) and ran on precast 4−20% Criterion TGX gels (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Clavibacter transformants were selected on TBY rich media supplemented with 200 μg/mL apramycin (Apr) and screened on TBY with only 100 μg/mL apramycin (Alfa Aesar, Haverhill, MA, USA). Escherichia coli was grown in Luria Broth (LB ...
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bioRxiv - Microbiology 2022Quote: ... This was followed by fixing the remaining adherent A549 cells in the wells with 4% formaldehyde in PBS for 10 min (Alfa Aesar, UK), permeabilizing the cells with 0.2% Triton-X100 (VWR ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were initially fixed in fresh 4% PFA for one hour at room temperature and washed with 1X PBS (Alfa Aesar, J62036) twice for two minutes each ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 × 105 cells were seeded onto glass coverslip (SLS, MIC3300) overnight before fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899) for 10min ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... difficile as described above were washed thrice with PBS to remove unadhered bacteria and fixed with 4% paraformaldehyde (PFA) (Alfa Aesar, USA) for 15 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded onto poly-L-lysine coated glass coverslip to reach 60% confluency before fixing with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar # J61899) for 10 min at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... or ER-mCherry-S1-2 (16 μg) and sonicated salmon sperm DNA (14 μg) were transfected into the cells with 240 μL of polyethyleneimine (#43896, Alfa Aesar, Ward Hill, MA, USA). After incubation of the cells for 24 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was composed of equal amounts of protein in addition to varying amounts of sodium dodecyl sulfate buffer (SDS sample buffer; Alfa Aesar, Ward Hill, MA, USA) supplemented with beta-mercapto-ethanol and water ...
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bioRxiv - Physiology 2024Quote: ... was mounted for histology at a 90° angle on a piece of cork in a 1:1 w/w mixture of optimal cutting temperature (OCT) solution and tragacanth powder (Alfa Aesar, Ward Hill, MA, USA). The mount was then covered in OCT ...
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bioRxiv - Physiology 2024Quote: ... was mounted for histology at a 90° angle on a piece of cork in a 1:1 w/w mixture of optimal cutting temperature (OCT) solution and tragacanth powder (Alfa Aesar, Ward Hill, MA, USA). The mount was then covered in OCT ...
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bioRxiv - Cancer Biology 2021Quote: ... S34F and Q157R HEL cells and not-infected HEL cells were labeled in duplicate with 100 μM of the uridine analog 4-thiouridine (s4U, Alfa Aesar, Cat #AAJ60679MC) for 2 hours at 37°C in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... were dissected in ice-cold Ca2+-free saline solution and fixed for 24 h in 4% paraformaldehyde (PFA; J19943, Alfa Aesar; in PBS) at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... VHHs were enzymatically biotinylated in their BAP tag by incubating 1 mg of purified VHHs with 10 µM of ATP (Alfa Aesar, Haverhill, MA, Cat#CAAAJ61125-09), 100 µM of D-(+)-biotin (VWR ...