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Citations for Alfa Aesar :
301 - 350 of 357 citations for 4 3 6 Dimethyl 3 heptyl phenol monoethoxylate ring 13C6 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 2.5 µg of plasmid DNA was mixed with 5 µL Polyethyleneimine (PEI) at 1ug/ml stock solution (Alfa Aesar) and incubated for 15 min at room temperature before being added to the RD114 cells ...
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bioRxiv - Systems Biology 2022Quote: ... The cells were transiently transfected with N-terminal YFP-fused NF2 variants (pdEYFP) and mutant proteins using polyethylenimine (PEI, 1 mg/ml, Alfa Aesar, 9002-98-6) at 1:5 ratio of pDNA:transfection reagent ...
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bioRxiv - Developmental Biology 2021Quote: ... Gastruloids were collected at 96 h after seeding and fixed with 4% paraformaldehyde (PFA, Alfa Aesar) overnight at 4 °C.
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bioRxiv - Microbiology 2020Quote: ... cells were treated with nocodazole after which they were fixed by 4% paraformaldehyde (PFA) (Alfa Aesar, Haverhill ...
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bioRxiv - Biochemistry 2021Quote: ... The media was removed and the cells were fixed using 4 % paraformaldehyde (Alfa Aesar, Haverhill, USA) (10min ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by addition of 100 µl N-methyl-trimethylsilyl-trifluoroacetamide (MSTFA) (Alfa Aesar, 24589-78-4) for 12 hours at room temperature25,26 ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was stopped by addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar). After boiling at 95 °C for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction was stopped by addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar). After boiling samples at 95 °C for 5 min ...
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bioRxiv - Genetics 2023Quote: ... and the cells were washed with cold 1XPBS and fixed using 4% PFA (Alfa Aesar #J61899). Cells were blocked for 1 hour using a blocking buffer composed of 1XPBS ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction was stopped by addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar). After boiling at 95 °C for 5 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 4-(2-Hydroxyethyl)-1-piperazinepropane-sulfonic acid (EPPS) was purchased from Alfa Aesar (Product no.: J60511). Two different type of poly acrylic acids were used (PAA) ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of remaining neutrophils was fixed with 4% Paraformaldehyde (PFA, cat.no. 43368, Alfa Aesar, USA) and then stored overnight at −80°C before transferred to and stored in liquid nitrogen.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with isopropanol (60%) for 5 minutes and then with ORO solution (ORO, 2mg/mL, Alfa Aesar, A12989) for 20 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and 75 μL of a pre-filtered solution of 1 mg/mL XTT sodium salt (Alfa Aesar, 111072-31-2) and 20 μL/mL XTT activator (Trevigen ...
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bioRxiv - Cancer Biology 2023Quote: ... and 75 µL of a pre-filtered solution of 1 mg/mL XTT sodium salt (Alfa Aesar, 111072-31-2) and 20 µL/mL XTT activator (Trevigen ...
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bioRxiv - Developmental Biology 2022Quote: Cells cultured in a µ-slide 8 well dish were fixed with 4% paraformaldehyde (J61899, Alfa Aesar) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction was stopped by the addition of 4 × reducing Laemmli SDS sample loading buffer (Alfa Aesar). After boiling at 95 °C for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... 3T3 fibroblast cells were fixed for 15 min in 4% paraformaldehyde (43368; Alfa Aesar, Haverhill, MA, USA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2020Quote: ... were impregnated with 5 μl of a freshly made stock antibiotic solution of either 40 mg/ml streptomycin sulfate (MilliporeSigma) in sterile distilled water or 10 mg/ml oxytetracycline hydrochloride (Alfa Aesar) in sterile distilled water to reach a concentration of 200 μg for streptomycin and 50 μg for oxytetracycline in each disc ...
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bioRxiv - Microbiology 2022Quote: ... was added directly to a tube containing 120 µL of a 5X fixation cocktail (100 μL of 16% (wt/vol) paraformaldehyde aqueous solution (methanol-free, Alfa Aesar) and 20 μL of 1 M NaPO4 buffer (pH 7.4) ...
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bioRxiv - Microbiology 2023Quote: ... Trypanosomes were lysed by the addition of 50 μl/well of lysis solution (30 mM Tris pH 7.5, 7.5 mM EDTA, 0.012% saponin (Alfa Aesar, Cat. No. A18820), and 0.12% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Cells were fixed for 30 minutes at room temperature in 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899). Cells were washed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were fixed with 4% paraformaldehyde (PFA; Electron Microscopy Services) with 0.075 mg/mL saponin (Alfa Aesar,Sigma) diluted in phosphate-buffered saline (PBS ...
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bioRxiv - Biophysics 2021Quote: ... 3T3 fibroblast and HUVEC cells were fixed for 15 min in 4% paraformaldehyde (43368; Alfa Aesar, Haverhill, MA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diluted with 30 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 95 °C for 5 min ...
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bioRxiv - Genomics 2022Quote: ... The infection was terminated by washing the cells with PBS and fixing them with 4% paraformaldehyde (Alfa Aesar) for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diluted with 30 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
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bioRxiv - Genetics 2023Quote: ... At the designated timepoints the supernatant was discarded and replaced with 4% PFA (cat.no. 43368, Alfa Aesar, USA). The plates were incubated at 4°C for 24h ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed three times with 200μL of FACS buffer and fixed with 200μL of 4% PFA (Alfa Aesar) for 15 mins at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... the cancer cell lines MCF7 and MDA-MB-231 were fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Haverhill, MA) for 15-30 min at room temperature following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice with phosphate buffered saline (PBS) at 37°C and fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar) at 37°C ...
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bioRxiv - Physiology 2022Quote: ... cells were washed twice for 5 min with PBS supplemented with 500 μM MgCl2 and 0.5 μM CaCl2 (PBS-S) then fixed with 4% paraformaldehyde (catalog no. 43368, Alfa Aesar) for 20 min and washed with PBS-S twice for 5 min ...
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bioRxiv - Immunology 2020Quote: ... BL21 cells were spun down (3000 RPM, 30 min, 4 °C) and resuspended in Tris-buffered saline (TBS, Alfa Aesar) containing cOmplete™ protease inhibitor cocktail (Sigma Millipore) ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 100 μL PBS and fixed by pipetting into 24 μL fixation cocktail (4 μL 1M NaPO4 buffer, pH 7.4, and 20 μL 16% (wt/vol) paraformaldehyde (Alfa Aesar)) ...
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bioRxiv - Biophysics 2020Quote: An aqueous solution of Alexa Fluor 488 (10−8 mol/L in H2O) was diluted 1:100 in a solution of polyvinyl alcohol (PVA, 98-99 % hydrolyzed, low molecular weight, Alfa Aesar; 1.5 % in H2O). A droplet (10 µL ...
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bioRxiv - Biophysics 2023Quote: ... cells and beads were suspended into a 0.6 w/w% solution of methyl cellulose (MC) (4000 cPs, Alfa Aesar 036718.22, CAS 9004-67-5) dissolved in phosphate buffered saline (PBS ...
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bioRxiv - Bioengineering 2022Quote: A volume of 1.5 mL of the collected biomass sample was resuspended using 13.5 mL of solution of a commercial 1% formaldehyde-phosphate-buffered saline (F-PBS) (Alfa Aesar - Thermo Fischer Scientific, USA) in a 15 mL Falcon tube under biosafety level II conditions ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatants were collected by centrifugation at 3,200 x g at 4°C for 30 min and the pH was adjusted to the same value as fresh media 5N KOH (Alfa Aesar) with the Mettler Toledo InLab Micro pH electrode ...
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bioRxiv - Neuroscience 2020Quote: ... adult mice were killed rapidly by cervical dislocation and the brains dissected and immediately post-fixed in 10 ml 4% paraformaldehyde (Alfa Aesar) in 0.1 M phosphate buffer for 4-5 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... or 300,000 (MDA-MB-231) cells were plated per dish and cells were fixed 18 h later with 4% paraformaldehyde (Alfa Aesar) for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... Incubations were quenched by diluting the incubation with 20 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
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bioRxiv - Biophysics 2023Quote: The tissue was fixed at the end of each experiment with 4% paraformaldehyde in PBS (Alfa Aesar, ThermoFisher GmbH, Kandel, Germany) for 5 min at room temperature followed by thoroughly washing using 1x Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Microbiology 2023Quote: ... of each sample were spotted onto the surface of Mueller-Hinton agar (30 ml in a standard 100 mm petri dish) containing 4 µg/ml ticarcillin-clavulanate (15:1, Alfa Aesar). Plates were incubated at 37°C for approximately 24 h.
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... an overnight culture was grown in 5 ml LB (E. coli MG1655 recipient) or 5 ml LB (E & O Laboratories Ltd) + 4 µg/ml cefotaxime (Alfa Aesar) (donors ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then boiled for 8 min at 90 °C after addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar) and ran on precast 4−20% Criterion TGX gels (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... Protein pellet was dissolved in RIPA buffer and then 20 μL of 4× reducing Laemmli SDS sample loading buffer (Cat. No. J63615, Alfa Aesar) was added and heated at 95 °C for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred to a new bottle and stored at 4 °C until use. N,N’-methylenebis(acrylamide) (BIS; catalog no. J66710) was purchased from Alfa Aesar. Ammonium persulfate (APS ...
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bioRxiv - Physiology 2024Quote: ... was mounted for histology at a 90° angle on a piece of cork in a 1:1 w/w mixture of optimal cutting temperature (OCT) solution and tragacanth powder (Alfa Aesar, Ward Hill, MA, USA). The mount was then covered in OCT ...
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bioRxiv - Physiology 2024Quote: ... was mounted for histology at a 90° angle on a piece of cork in a 1:1 w/w mixture of optimal cutting temperature (OCT) solution and tragacanth powder (Alfa Aesar, Ward Hill, MA, USA). The mount was then covered in OCT ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... difficile as described above were washed thrice with PBS to remove unadhered bacteria and fixed with 4% paraformaldehyde (PFA) (Alfa Aesar, USA) for 15 min at RT ...