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Citations for Alfa Aesar :
251 - 300 of 303 citations for 7 Azabicyclo 2.2.1 heptane 7 carboxylic acid 2 phenylmethoxy carbonyl amino 1 1 dimethylethyl ester 1R 2S 4S rel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and 66mg of corresponding beads of the same size (200-400 mesh) and material (polystyrene divinylbenzene 1% cross linked beads; Alfa Aesar) were used for mock depletion ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial strains carrying plasmids were maintained using the corresponding antibiotics at the indicated concentrations: 10 µg mL−1 of thiamphenicol (Tm; Alfa Aesar), 50 µg mL−1 of kanamycin (Kan ...
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bioRxiv - Developmental Biology 2022Quote: Zinc and copper IUDs were made from 1 cm of 99.994% pure zinc and 99.999% pure copper wire (Alfa Aesar, MA, USA), 0.5 mm in diameter ...
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bioRxiv - Microbiology 2021Quote: ... sample volume was adjusted such that approximately 107 cells were harvested and re-suspended in 1 x Hepes Buffered Saline (5X HBS; Alfa Aesar). Cells were washed and resuspended in 1 mL HBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Trypticase-Yeast extract-Glucose (TYG) media or Defined Minimal Media (DMM) supplemented with 1 mg/L resazurin sodium salt (Alfa Aesar), 10 mg/L hemin (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... to stain the membrane and the cell wall was labeled with 1 μg/ml of an equal mixture of vancomycin (Alfa Aesar) and a BODIPY FL conjugate of vancomycin (Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: ... For the experiments in Heptanol: the flowing medium was replaced with HM containing 300 μl/l of Heptanol (1-Heptanol, 99%, Alfa Aesar) after well mixing ...
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bioRxiv - Microbiology 2023Quote: ... of each sample were spotted onto the surface of Mueller-Hinton agar (30 ml in a standard 100 mm petri dish) containing 4 µg/ml ticarcillin-clavulanate (15:1, Alfa Aesar). Plates were incubated at 37°C for approximately 24 h.
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bioRxiv - Cell Biology 2022Quote: ... TA and GM muscles were dissected and partially embedded in a plastic cassette holder using 1 g/ml of tragacanth powder gum (Alfa Aesar) in water ...
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bioRxiv - Immunology 2023Quote: ... BG-agarose beads were demulsified by addition of 800 μL PBS and 200 μL 1H,1H,2H,2H-perfluoro-1-octanol (PFO, Alfa Aesar), filtered through a 50 μm strainer and washed once in 15 mL FACS buffer (1% ultra-low IgG FBS (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... Inocula for soil-derived enrichment cultures were generated from soil (2.5 g) resuspended in sterile 1×PBS (25 mL) supplemented with 2.24 mM sodium pyrophosphate (Alfa Aesar, Heysham, England) and stirred for 30 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... aminosilation of coverslips were carried out in a mixture of 100 mL water and 1% (v/v) aminopropylsilane (Alfa Aesar, A10668, UK). Third ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 800 µL TE buffer (10 mM Tris pH 8.0, 1 mM EDTA) + 15 mg/mL lysozyme (Alfa Aesar J60701-06) and incubated at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed in the digitonin lysis buffer (1% digitonin [Sigma-Aldrich D141], 137 mM NaCl, 10% w/v glycerol [Alfa Aesar 38988] ...
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bioRxiv - Biophysics 2021Quote: ... We then detergent extracted the cells by resuspending the cell pellet in PEME buffer with 1% IGEPAL (CA-630, Alfa Aesar, MA) and incubating on ice for 15 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... and FUCCI plasmid-transduced cells were selected with 2 μg/mL blasticidin (Alfa Aesar). Upon completion of drug selection ...
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bioRxiv - Cancer Biology 2022Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (Alfa Aesar, L11939, lot no. 10196058) dissolved in culture medium was added to each well and incubated for 2-3hrs at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Half of the culture dishes were fixed with 2% paraformaldehyde (PFA) (PFA Alfa Aesar)/ 0.075% glutaraldehyde (GA ...
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bioRxiv - Cell Biology 2024Quote: Cells were washed twice in EBM-2 and fixed in 4% paraformaldehyde (Alfa Aesar) diluted in EBM-2 for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... the cell pellet obtained from initial centrifugation was incubated at 37°C for 1 hour with 300 rpm mixing in 250 μL 3 mg/mL lysozyme (Alfa Aesar, Haverhill, MA) in Tris-EDTA buffer (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... Cal-520-AM-loaded cells were removed from the CO2 incubator and equilibrated at room temperature for 5 minutes prior to IP3 stimulation by the addition of 200 µl of 1 mM carbachol (Alfa Aesar; #L06674-06), a Gαq-coupled M3 muscarinic receptor agonist ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PFOA (CAS 335-67-1, purity ≥ 95%), and HFPO-DA/GenX (CAS 1352-13-6, purity ≥ 97%) were from Alfa Aesar (Havermill, MA). Perfluorohexanoic acid (PFHxA ...
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bioRxiv - Microbiology 2020Quote: ... 1.0 ml of a 10 mM solution of Fe(CIO4)2 (Alfa Aesar, Tewksbury, MA) in 0.1M HClO4 (Ricca Chemical ...
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bioRxiv - Microbiology 2022Quote: ... for 2 min and staining nuclei of the adherent A549 cells with DAPI (Alfa Aesar) at 300 nM for 5 min ...
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bioRxiv - Biophysics 2020Quote: An aqueous solution of Alexa Fluor 488 (10−8 mol/L in H2O) was diluted 1:100 in a solution of polyvinyl alcohol (PVA, 98-99 % hydrolyzed, low molecular weight, Alfa Aesar; 1.5 % in H2O). A droplet (10 µL ...
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bioRxiv - Neuroscience 2020Quote: ... 0.04% w/v red dye (Sulforhodamine B, MP Biochemicals, CAS# 3520-42-1) and 0.02% w/v blue dye (Erioglaucine A, Alfa Aesar, CAS# 3844-45-9) were used for food coloring ...
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bioRxiv - Biochemistry 2020Quote: ... two sequential 24-hour pre-cultures were grown on 1 % ethanol minimal medium (EMM) media containing 15 and 10 nM β-estradiol (Alfa Aesar, Th. Geyer), respectively ...
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bioRxiv - Bioengineering 2022Quote: A volume of 1.5 mL of the collected biomass sample was resuspended using 13.5 mL of solution of a commercial 1% formaldehyde-phosphate-buffered saline (F-PBS) (Alfa Aesar - Thermo Fischer Scientific, USA) in a 15 mL Falcon tube under biosafety level II conditions ...
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bioRxiv - Systems Biology 2022Quote: ... The cells were transiently transfected with N-terminal YFP-fused NF2 variants (pdEYFP) and mutant proteins using polyethylenimine (PEI, 1 mg/ml, Alfa Aesar, 9002-98-6) at 1:5 ratio of pDNA:transfection reagent ...
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bioRxiv - Cell Biology 2019Quote: ... infected cells were selected by incubation in growth medium containing puromycin (2 μg/ml; Alfa Aesar) for 1 week ...
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bioRxiv - Bioengineering 2021Quote: ... VHHs were enzymatically biotinylated in their BAP tag by incubating 1 mg of purified VHHs with 10 µM of ATP (Alfa Aesar, Haverhill, MA, Cat#CAAAJ61125-09), 100 µM of D-(+)-biotin (VWR ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cells were then washed with 25 mL of ice cold STM buffer (pH = 7.5, 270 mM sucrose, Millipore Sigma, 1.07651.5000, 10 mM Tris HCl, Alfa Aesar, J67233, 1 mM MgCl2, VWR, E525-100ML) and spun down again at 4°C for 5 minutes at 1500 x g ...
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bioRxiv - Bioengineering 2019Quote: DSS-treated mice received 3 cycles of 2% (weight/volume) DSS (40,000 MW, Alfa Aesar, Thermo Fisher, France) in drinking water for 5 days ...
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bioRxiv - Immunology 2021Quote: Viruses and control recombinant SARS-CoV-2 spike protein (LakePharma) were denatured with Laemmli sample buffer (Alfa Aesar) by heating at 95 °C for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: The synthesis began with a reaction of commercially available N-Boc-L-tyrosine (2 mmol, Alfa Aesar, 98%) and oleylamine (2.4 mmol ...
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bioRxiv - Bioengineering 2023Quote: ... coverslips were acryl-silanised submerged for 2 hrs in a 0.5% solution of 3-(Acryloyloxy)propyltrimethoxysilane (Alfa Aesar) in ethanol with 5% water ...
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bioRxiv - Molecular Biology 2020Quote: ... MTT (3-[4, 5- dimethyl thiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (Alfa Aesar Chemicals Co. Ltd. Shanghai, China), fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2019Quote: ... and then simultaneously blocked and permeabilized with 2% BSA in PBS++ with 0.1% saponin (Alfa Aesar, cat. no. A18820) for 1hr at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... each sample was diluted to 2 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
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bioRxiv - Cell Biology 2022Quote: ... mice were anesthetized and sacrificed by transcardial perfusion with 2 % paraformaldehyde (PFA, Cat.# J61984 Alfa Aesar, Haverhill, United States). Tumors were dissected and fixed with 4 % PFA for 24-48 hours and frozen in the fume of liquid nitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The medium was then replaced by the normal cell culture medium supplemented with 2 mM L-methionine (Alfa Aesar J61904) for 0-3 hr at 37°C for the chase ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with 0.5 mg/ml of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Alfa Aesar, Haverhill, MA) containing media for 3.5 h ...
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bioRxiv - Developmental Biology 2019Quote: ... Next each sample was diluted to 2 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
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bioRxiv - Cell Biology 2022Quote: ... that was threaded through a heated platinum coil (∼2 mm in diam.; Pt wire is 0.3 mm in diam., Alfa Aesar) upon application of a desired voltage ...
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bioRxiv - Biochemistry 2019Quote: ... UK) for 2 mins at 37°C prior to the addition of calcium chloride (25 μl, 50 mM; Alfa Aesar, UK). The time taken for clot formations to occur (an upper maximal of 2 mins was imposed ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were grown to confluency and then screened based on functional testing of the XBP1s construct using RT-PCR (described below) with or without 2 μg/mL doxycycline (dox; Alfa Aesar). The selected SupT1 single colony cell line encoding tetracycline-inducible XBP1s was then transduced with lentivirus encoding DHFR.ATF6(1–373 ...
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bioRxiv - Biochemistry 2022Quote: ... FDH2 was assayed in an argon (Airgas AR-300) atmosphere with 2 mM or 20 mM Benzyl viologen (Alfa Aesar H66836) in 50 mM Tris pH 8.0 with 20 mM glucose and supplemented with glucose oxidase (Sigma-G7141-50ku ...
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bioRxiv - Bioengineering 2022Quote: ... TLS sample was prepared in 250 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP HCl, Cat# H51864, Alfa Aesar, Haverhill, MA, USA) in PBS.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The transfection mixture was prepared in Opti-MEM media with a ratio of 2 μL of PEI (Alfa Aesar, 1mg/mL) per 1 μg of DNA ...
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bioRxiv - Microbiology 2022Quote: ... 1μg gRNA and 3μg of pCTR4-2-FKS1 promoter construct (48) and cells were plated on YPD with 400μg/mL hygromycin B (Alfa Aesar, Cat# J69681). Transformants were verified with PCR spanning the joint between the promoter construct and FKS1 coding sequence and tested for response to copper by growth assay and gene expression.