Labshake search
Citations for Alfa Aesar :
251 - 300 of 303 citations for 6 Chloro N methyl 5 nitro 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: 23 DIV neurons were fixed in 4 % (w/v) paraformaldehyde in phosphate-buffered saline (PBS) (Alfa Aesar, MA, USA) and non-specific fluorescence quenched by incubation in 50 mM NH4Cl in PBS for 10 minutes ...
-
bioRxiv - Bioengineering 2023Quote: ... the cancer cell lines MCF7 and MDA-MB-231 were fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Haverhill, MA) for 15-30 min at room temperature following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... Acute sterol depletion was achieved by 1 h incubation with 5 mM MβCD (Alfa Aesar, 1303.31 g/mol) at 37°C ...
-
bioRxiv - Plant Biology 2022Quote: ... Lyophilized samples were dissolved in a buffer consisting of 5 mM MOPS sodium salt (Alfa Aesar, MA, USA) and water ...
-
bioRxiv - Microbiology 2022Quote: ... the agar was supplemented with hemin (5 μg/mL) (MilliporeSigma) and vitamin K1 (1 μg/mL) (Alfa Aesar) and plates were poured ...
-
bioRxiv - Microbiology 2023Quote: ... fragilis NCTC 9343 was grown in Brain heart infusion (BHIS) supplemented with 5 mg/L hemin (Alfa Aesar) in 1 N NaOH ...
-
bioRxiv - Microbiology 2023Quote: ... fragilis NCTC 9343 was grown in Brain heart infusion (BHIS) supplemented with 5 mg/L hemin (Alfa Aesar) in 1 N NaOH ...
-
bioRxiv - Plant Biology 2020Quote: ... All samples were calibrated using a matrix-matched serial dilution of Specpure multi-element plasma standard solution 4 (Alfa Aesar) set by linear regression.
-
bioRxiv - Plant Biology 2020Quote: ... All samples were calibrated using a matrix-matched serial dilution of Specpure multi-element plasma standard solution 4 (Alfa Aesar) set by linear regression.
-
bioRxiv - Cell Biology 2021Quote: ... washed twice with phosphate buffered saline (PBS) at 37°C and fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar) at 37°C ...
-
bioRxiv - Physiology 2022Quote: ... cells were washed twice for 5 min with PBS supplemented with 500 μM MgCl2 and 0.5 μM CaCl2 (PBS-S) then fixed with 4% paraformaldehyde (catalog no. 43368, Alfa Aesar) for 20 min and washed with PBS-S twice for 5 min ...
-
bioRxiv - Immunology 2020Quote: ... BL21 cells were spun down (3000 RPM, 30 min, 4 °C) and resuspended in Tris-buffered saline (TBS, Alfa Aesar) containing cOmplete™ protease inhibitor cocktail (Sigma Millipore) ...
-
bioRxiv - Biophysics 2020Quote: ... Young adult worms were placed on NG agar plates containing 4 mM auxin (Indole-3 acetic acid, Alfa Aesar, # A10556) seeded with E ...
-
bioRxiv - Cell Biology 2023Quote: ... Propidium iodide was used as a dead cell marker (1mg/ml aqueous solution; 25535-16-4, Alfa Aesar, Lancashire, UK). Cells were then sorted using fluorescence-activated cell sorting (BD Biosciences ...
-
bioRxiv - Microbiology 2024Quote: ... resuspended in 100 μL PBS and fixed by pipetting into 24 μL fixation cocktail (4 μL 1M NaPO4 buffer, pH 7.4, and 20 μL 16% (wt/vol) paraformaldehyde (Alfa Aesar)) ...
-
bioRxiv - Genetics 2019Quote: ... the 4 ml cultures were grown in the presence or absence of 0.5 mM of orsellinic acid (Alfa Aesar, Heysham, England) in DMSO.
-
bioRxiv - Synthetic Biology 2021Quote: ... The supernatants were collected by centrifugation at 3,200 x g at 4°C for 30 min and the pH was adjusted to the same value as fresh media 5N KOH (Alfa Aesar) with the Mettler Toledo InLab Micro pH electrode ...
-
bioRxiv - Neuroscience 2020Quote: ... adult mice were killed rapidly by cervical dislocation and the brains dissected and immediately post-fixed in 10 ml 4% paraformaldehyde (Alfa Aesar) in 0.1 M phosphate buffer for 4-5 h at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... or 300,000 (MDA-MB-231) cells were plated per dish and cells were fixed 18 h later with 4% paraformaldehyde (Alfa Aesar) for 10 min ...
-
bioRxiv - Neuroscience 2020Quote: ... pups of both sexes were euthanized with an overdose (>200mg/kg) of 20% pentobarbital sodium solution and transcardially perfused with 4% paraformaldehyde (PFA, Alfa Aesar) in phosphate-buffered solution (PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... transcardiac perfusion of phosphate buffered saline (PBS; Oxoid) was performed and followed by transcardiac perfusion of 4% (v/v) paraformaldehyde (PFA; Alfa Aesar). PBS and PFA volumes used were ...
-
bioRxiv - Cell Biology 2019Quote: ... mice were sacrificed following institutional guidelines and solidified Matrigel plugs were excised and fixed using 4% paraformaldehyde (Alfa Aesar, AA43368-9M). Plugs were cryosectioned ...
-
bioRxiv - Biochemistry 2020Quote: ... Incubations were quenched by diluting the incubation with 20 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
-
bioRxiv - Genetics 2020Quote: ... and auxin treatment was performed by transferring worms to bacteria-seeded plates containing 4 mM natural auxin (indole-3-acetic acid; Alfa Aesar). Worms were kept on these plates for 4 h (germline depletion ...
-
bioRxiv - Microbiology 2023Quote: ... of each sample were spotted onto the surface of Mueller-Hinton agar (30 ml in a standard 100 mm petri dish) containing 4 µg/ml ticarcillin-clavulanate (15:1, Alfa Aesar). Plates were incubated at 37°C for approximately 24 h.
-
bioRxiv - Cancer Biology 2023Quote: ... Protein pellet was dissolved in RIPA buffer and then 20 μL of 4× reducing Laemmli SDS sample loading buffer (Cat. No. J63615, Alfa Aesar) was added and heated at 95 °C for 5 min ...
-
bioRxiv - Biophysics 2023Quote: The tissue was fixed at the end of each experiment with 4% paraformaldehyde in PBS (Alfa Aesar, ThermoFisher GmbH, Kandel, Germany) for 5 min at room temperature followed by thoroughly washing using 1x Dulbecco’s Phosphate Buffered Saline (DPBS ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were then boiled for 8 min at 90 °C after addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar) and ran on precast 4−20% Criterion TGX gels (Bio-Rad).
-
bioRxiv - Neuroscience 2019Quote: ... animals were mounted onto 5% agarose pads and immobilized using 0.5µl of 0.10 µmpolystyrene latex microsphere (Alfa Aesar # 427124Y). The coverslip was sealed with Vaseline (Vaseline® Jelly Original) ...
-
bioRxiv - Developmental Biology 2019Quote: ... The samples were diluted to 0.5 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
-
bioRxiv - Developmental Biology 2021Quote: ... The samples were diluted to 0.5 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
-
bioRxiv - Developmental Biology 2021Quote: ... each sample was diluted to 2 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
-
bioRxiv - Immunology 2023Quote: ... 2.5 µg of plasmid DNA was mixed with 5 µL Polyethyleneimine (PEI) at 1ug/ml stock solution (Alfa Aesar) and incubated for 15 min at room temperature before being added to the RD114 cells ...
-
bioRxiv - Microbiology 2022Quote: ... This was followed by fixing the remaining adherent A549 cells in the wells with 4% formaldehyde in PBS for 10 min (Alfa Aesar, UK), permeabilizing the cells with 0.2% Triton-X100 (VWR ...
-
bioRxiv - Neuroscience 2022Quote: ... tadpoles were washed three times with PBS (-/-) and fixed in 4% Paraformaldehyde in PBS + Mg + EGTA (MEMFA) (Alfa Aesar, Tewksbury, MA, USA) for 2 hours at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... Slides were initially fixed in fresh 4% PFA for one hour at room temperature and washed with 1X PBS (Alfa Aesar, J62036) twice for two minutes each ...
-
Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... difficile as described above were washed thrice with PBS to remove unadhered bacteria and fixed with 4% paraformaldehyde (PFA) (Alfa Aesar, USA) for 15 min at RT ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 × 105 cells were seeded onto glass coverslip (SLS, MIC3300) overnight before fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899) for 10min ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with isopropanol (60%) for 5 minutes and then with ORO solution (ORO, 2mg/mL, Alfa Aesar, A12989) for 20 min ...
-
bioRxiv - Developmental Biology 2019Quote: ... Next each sample was diluted to 2 M Guanidine HCl with 5 mM EPPS (pH 9.0, Alfa Aesar cat# A13714), ensuring that the pH was at least 8.5 ...
-
bioRxiv - Cancer Biology 2021Quote: ... S34F and Q157R HEL cells and not-infected HEL cells were labeled in duplicate with 100 μM of the uridine analog 4-thiouridine (s4U, Alfa Aesar, Cat #AAJ60679MC) for 2 hours at 37°C in the dark ...
-
bioRxiv - Neuroscience 2022Quote: ... were dissected in ice-cold Ca2+-free saline solution and fixed for 24 h in 4% paraformaldehyde (PFA; J19943, Alfa Aesar; in PBS) at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were blocked at RT with blocking buffer (5% skim milk powder in TBST containing sodium orthovanadate (Na3VO4, 1mM, Alfa Aesar) and sodium fluoride (NaF ...
-
bioRxiv - Microbiology 2023Quote: ... cells were harvested by centrifugation (4720 x g for 5 min) and resuspended in filter-sterilised HEPES-buffered saline (HBS, Alfa Aesar). Diluted cell suspensions were added to tubes containing HBS ...
-
bioRxiv - Genomics 2021Quote: ... green bean size frozen tissue incubated in the ice-cold 800 µl of 1X homogenization unstable buffer(5 mM CaCl2 (Alfa Aesar, J63122), 3 mM Mg(Ac)2 (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2021Quote: ... each consisting of 5 days of DSS in drinking water (2%, 40 kDa) followed by 7 days of recovery (Alfa Aesar #J63606).
-
bioRxiv - Plant Biology 2023Quote: ... PCR tubes containing 7-day-old seedlings were transferred to a 3D-printed holder made to hold up to 5 seedlings and platinum-iridium foil electrodes (Platinum:Iridium = 80:20; Alfa Aesar 41805.FF), as previously described (Salvalaio et al. ...
-
bioRxiv - Microbiology 2021Quote: ... Overnight cultures of donor strain were inoculated from cryopreserved stocks (−80°C in LB + 10% glycerol) into 5 mL LB amended with 10 μg/mL kanamycin and 150 μL of 100 mM DAP (Alfa Aesar, Haverhill, MA) and incubated shaking at 37 °C ...
-
bioRxiv - Microbiology 2019Quote: ... F1 mice at 7 weeks of age (7-11/treatment group) were treated for 5 consecutive days with 1.5% DSS (Alfa Aesar, Haverhill, MA, USA) in sterile drinking water ...
-
bioRxiv - Neuroscience 2022Quote: ... were pulled to a shank length of approximately 0.6 cm (5-8 MΩ) using a Narishige PC-10 puller (Tokyo, Japan) and filled with 2% Pontamine sky blue dye (Alfa Aesar, Haverhill, MA) dissolved in sodium acetate (0.5 M ...