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Citations for Alfa Aesar :
151 - 200 of 201 citations for 4 4 Bromophenylsulfonyl morpholine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... 4-(2-Hydroxyethyl)-1-piperazinepropane-sulfonic acid (EPPS) was purchased from Alfa Aesar (Product no.: J60511). Two different type of poly acrylic acids were used (PAA) ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was stopped by addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar). After boiling at 95 °C for 5 min ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of remaining neutrophils was fixed with 4% Paraformaldehyde (PFA, cat.no. 43368, Alfa Aesar, USA) and then stored overnight at −80°C before transferred to and stored in liquid nitrogen.
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bioRxiv - Biochemistry 2023Quote: ... The reaction was stopped by addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar). After boiling samples at 95 °C for 5 min ...
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bioRxiv - Genetics 2023Quote: ... and the cells were washed with cold 1XPBS and fixed using 4% PFA (Alfa Aesar #J61899). Cells were blocked for 1 hour using a blocking buffer composed of 1XPBS ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction was stopped by addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar). After boiling at 95 °C for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: Cells cultured in a µ-slide 8 well dish were fixed with 4% paraformaldehyde (J61899, Alfa Aesar) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were then washed 3 times in cold PBS and fixed in cold 4% paraformaldehyde (Alfa Aesar) in PBS for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction was stopped by the addition of 4 × reducing Laemmli SDS sample loading buffer (Alfa Aesar). After boiling at 95 °C for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... 3T3 fibroblast cells were fixed for 15 min in 4% paraformaldehyde (43368; Alfa Aesar, Haverhill, MA, USA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: ... Cells were fixed for 30 minutes at room temperature in 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899). Cells were washed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were fixed with 4% paraformaldehyde (PFA; Electron Microscopy Services) with 0.075 mg/mL saponin (Alfa Aesar,Sigma) diluted in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... the coverslips were washed 3 times with PBS and fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Heysham, England) in PBS for 20 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... 3T3 fibroblast and HUVEC cells were fixed for 15 min in 4% paraformaldehyde (43368; Alfa Aesar, Haverhill, MA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Genomics 2022Quote: ... The infection was terminated by washing the cells with PBS and fixing them with 4% paraformaldehyde (Alfa Aesar) for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diluted with 30 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
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bioRxiv - Genetics 2023Quote: ... At the designated timepoints the supernatant was discarded and replaced with 4% PFA (cat.no. 43368, Alfa Aesar, USA). The plates were incubated at 4°C for 24h ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then diluted with 30 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 95 °C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... the coverslips were washed 3 times with PBS and cells fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Heysham, England) in PBS for 20 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... MTT (3-[4, 5- dimethyl thiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (Alfa Aesar Chemicals Co. Ltd. Shanghai, China), fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed three times with 200μL of FACS buffer and fixed with 200μL of 4% PFA (Alfa Aesar) for 15 mins at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... the cancer cell lines MCF7 and MDA-MB-231 were fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Haverhill, MA) for 15-30 min at room temperature following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were calibrated using a matrix-matched serial dilution of Specpure multi-element plasma standard solution 4 (Alfa Aesar) set by linear regression.
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bioRxiv - Plant Biology 2020Quote: ... All samples were calibrated using a matrix-matched serial dilution of Specpure multi-element plasma standard solution 4 (Alfa Aesar) set by linear regression.
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bioRxiv - Cell Biology 2021Quote: ... washed twice with phosphate buffered saline (PBS) at 37°C and fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar) at 37°C ...
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bioRxiv - Physiology 2022Quote: ... cells were washed twice for 5 min with PBS supplemented with 500 μM MgCl2 and 0.5 μM CaCl2 (PBS-S) then fixed with 4% paraformaldehyde (catalog no. 43368, Alfa Aesar) for 20 min and washed with PBS-S twice for 5 min ...
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bioRxiv - Immunology 2020Quote: ... BL21 cells were spun down (3000 RPM, 30 min, 4 °C) and resuspended in Tris-buffered saline (TBS, Alfa Aesar) containing cOmplete™ protease inhibitor cocktail (Sigma Millipore) ...
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bioRxiv - Biophysics 2020Quote: ... Young adult worms were placed on NG agar plates containing 4 mM auxin (Indole-3 acetic acid, Alfa Aesar, # A10556) seeded with E ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 100 μL PBS and fixed by pipetting into 24 μL fixation cocktail (4 μL 1M NaPO4 buffer, pH 7.4, and 20 μL 16% (wt/vol) paraformaldehyde (Alfa Aesar)) ...
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bioRxiv - Cell Biology 2023Quote: ... Propidium iodide was used as a dead cell marker (1mg/ml aqueous solution; 25535-16-4, Alfa Aesar, Lancashire, UK). Cells were then sorted using fluorescence-activated cell sorting (BD Biosciences ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatants were collected by centrifugation at 3,200 x g at 4°C for 30 min and the pH was adjusted to the same value as fresh media 5N KOH (Alfa Aesar) with the Mettler Toledo InLab Micro pH electrode ...
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bioRxiv - Neuroscience 2020Quote: ... adult mice were killed rapidly by cervical dislocation and the brains dissected and immediately post-fixed in 10 ml 4% paraformaldehyde (Alfa Aesar) in 0.1 M phosphate buffer for 4-5 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... or 300,000 (MDA-MB-231) cells were plated per dish and cells were fixed 18 h later with 4% paraformaldehyde (Alfa Aesar) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... pups of both sexes were euthanized with an overdose (>200mg/kg) of 20% pentobarbital sodium solution and transcardially perfused with 4% paraformaldehyde (PFA, Alfa Aesar) in phosphate-buffered solution (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Incubations were quenched by diluting the incubation with 20 μL of 4 x reducing Laemmli SDS sample loading buffer (Alfa Aesar) and heated at 90 °C for 5 min ...
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bioRxiv - Genetics 2020Quote: ... and auxin treatment was performed by transferring worms to bacteria-seeded plates containing 4 mM natural auxin (indole-3-acetic acid; Alfa Aesar). Worms were kept on these plates for 4 h (germline depletion ...
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bioRxiv - Microbiology 2023Quote: ... of each sample were spotted onto the surface of Mueller-Hinton agar (30 ml in a standard 100 mm petri dish) containing 4 µg/ml ticarcillin-clavulanate (15:1, Alfa Aesar). Plates were incubated at 37°C for approximately 24 h.
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bioRxiv - Cancer Biology 2023Quote: ... Protein pellet was dissolved in RIPA buffer and then 20 μL of 4× reducing Laemmli SDS sample loading buffer (Cat. No. J63615, Alfa Aesar) was added and heated at 95 °C for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred to a new bottle and stored at 4 °C until use. N,N’-methylenebis(acrylamide) (BIS; catalog no. J66710) was purchased from Alfa Aesar. Ammonium persulfate (APS ...
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bioRxiv - Biophysics 2023Quote: The tissue was fixed at the end of each experiment with 4% paraformaldehyde in PBS (Alfa Aesar, ThermoFisher GmbH, Kandel, Germany) for 5 min at room temperature followed by thoroughly washing using 1x Dulbecco’s Phosphate Buffered Saline (DPBS ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... an overnight culture was grown in 5 ml LB (E. coli MG1655 recipient) or 5 ml LB (E & O Laboratories Ltd) + 4 µg/ml cefotaxime (Alfa Aesar) (donors ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then boiled for 8 min at 90 °C after addition of 4×reducing Laemmli SDS sample loading buffer (Alfa Aesar) and ran on precast 4−20% Criterion TGX gels (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... This was followed by fixing the remaining adherent A549 cells in the wells with 4% formaldehyde in PBS for 10 min (Alfa Aesar, UK), permeabilizing the cells with 0.2% Triton-X100 (VWR ...
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bioRxiv - Neuroscience 2022Quote: ... tadpoles were washed three times with PBS (-/-) and fixed in 4% Paraformaldehyde in PBS + Mg + EGTA (MEMFA) (Alfa Aesar, Tewksbury, MA, USA) for 2 hours at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were initially fixed in fresh 4% PFA for one hour at room temperature and washed with 1X PBS (Alfa Aesar, J62036) twice for two minutes each ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 × 105 cells were seeded onto glass coverslip (SLS, MIC3300) overnight before fixed with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899) for 10min ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... difficile as described above were washed thrice with PBS to remove unadhered bacteria and fixed with 4% paraformaldehyde (PFA) (Alfa Aesar, USA) for 15 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded onto poly-L-lysine coated glass coverslip to reach 60% confluency before fixing with 4% paraformaldehyde (PFA) in PBS (Alfa Aesar # J61899) for 10 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... S34F and Q157R HEL cells and not-infected HEL cells were labeled in duplicate with 100 μM of the uridine analog 4-thiouridine (s4U, Alfa Aesar, Cat #AAJ60679MC) for 2 hours at 37°C in the dark ...
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bioRxiv - Microbiology 2021Quote: ... all dishes were incubated at 37°C with 5% CO2 before being washed with sterile PBS and fixed with 4% paraformaldehyde (Alfa Aesar, Tewksbury, MA). Confocal laser scanning microscopy (CLSM ...