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Citations for Alfa Aesar :
101 - 150 of 157 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... for 2 min and staining nuclei of the adherent A549 cells with DAPI (Alfa Aesar) at 300 nM for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... infected cells were selected by incubation in growth medium containing puromycin (2 μg/ml; Alfa Aesar) for 1 week ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated at 37°C for 30 minutes in 5 μM propidium iodide (Alfa Aesar) and 1:1500 Hoechst 33342 (Molecular Probes ...
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bioRxiv - Microbiology 2020Quote: ... cells were treated with nocodazole after which they were fixed by 4% paraformaldehyde (PFA) (Alfa Aesar, Haverhill ...
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bioRxiv - Microbiology 2020Quote: ... Cells were fixed in a final concentration of 1% paraformaldehyde (Alfa Aesar 16% W/V #43368) for 15 min ...
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bioRxiv - Biochemistry 2021Quote: ... The media was removed and the cells were fixed using 4 % paraformaldehyde (Alfa Aesar, Haverhill, USA) (10min ...
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bioRxiv - Cancer Biology 2020Quote: ... Rho Zero cell culture media was additionally supplemented with 50 μg/mL uridine (A15227.06, Alfa Aesar).
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bioRxiv - Cell Biology 2022Quote: ... media was aspirated and cells were incubated in room temperature Krebs-Ringer Buffer (Alfa Aesar #J67795) for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... and cells were lysed using 100 µL 0.1% Triton X-100 (Alfa Aesar, Ward Hill, MA) in PBS for 5 minutes at room temperature by gentle pipetting ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with 100 μl of a 5 mg/mL MTT solution (Alfa Aesar, L11939) for 3 hours ...
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bioRxiv - Genetics 2023Quote: ... and the cells were washed with cold 1XPBS and fixed using 4% PFA (Alfa Aesar #J61899). Cells were blocked for 1 hour using a blocking buffer composed of 1XPBS ...
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bioRxiv - Immunology 2024Quote: ... CAL-1 cells were treated for 5 days with 100 μM 2’,3’-dideoxycytidine (Alfa Aesar) in medium supplemented with 50 μg/ml uridine (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... NIH3T3 cells were cultured for 4 hours in the presence of 100 nM Bafilomycin A (Alfa Aesar) to block autophagy and/or in DMEM media without glutamine and serum for autophagy induction prior to downstream analysis ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in presence of ultrapure Zn sulfate monohydrate purchased from Alfa Aesar (Haverhill, MA. #1113809) at 5μM ...
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bioRxiv - Developmental Biology 2022Quote: Cells cultured in a µ-slide 8 well dish were fixed with 4% paraformaldehyde (J61899, Alfa Aesar) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: Unless otherwise stated all cell lysates were made by direct lysis in 6X Laemmli buffer (Alfa Aesar) to inactive SENPs and denature proteins followed by heating at 95 °C for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: Cells were fixed using 3.7% (v/v) formaldehyde then permeabilised using 0.1% Triton X-100 (Alfa Aesar) for 30min ...
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bioRxiv - Biophysics 2023Quote: ... 3T3 fibroblast cells were fixed for 15 min in 4% paraformaldehyde (43368; Alfa Aesar, Haverhill, MA, USA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were cultured in differentiation medium supplemented with 0.125 mM indomethacin (cat#A19910-06, Alfa Aesar), 0.5 µM dexamethasone (cat#d4902 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed for 10 minutes with room temperature (RT) 3.5% PFA (paraformaldehyde, pH 6.7; Alfa Aesar). Cells were then washed 3 x with Tris buffered saline (TBS ...
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bioRxiv - Immunology 2021Quote: ... Cells were fixed for 30 minutes at room temperature in 4% paraformaldehyde (PFA) in PBS (Alfa Aesar, J61899). Cells were washed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were fixed with 4% paraformaldehyde (PFA; Electron Microscopy Services) with 0.075 mg/mL saponin (Alfa Aesar,Sigma) diluted in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2019Quote: ... A 100 μL aliquot of this cell suspension was added to 900 μL of a chitin (Alfa Aesar) IO mixture (8 g/L chitin) ...
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bioRxiv - Microbiology 2020Quote: ... a 500 μl aliquot of cells in growth medium was added to 100 μl 16% paraformaldehyde (Alfa Aesar) and 20 μl of 1M NaPO4 buffer (pH 7.4) ...
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bioRxiv - Biophysics 2021Quote: ... 3T3 fibroblast and HUVEC cells were fixed for 15 min in 4% paraformaldehyde (43368; Alfa Aesar, Haverhill, MA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Genomics 2022Quote: ... The infection was terminated by washing the cells with PBS and fixing them with 4% paraformaldehyde (Alfa Aesar) for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were trypsinized and washed twice with PBS and lysed in 2x Laemmli Sample buffer (Alfa Aesar, J61337). For cytosolic lysate ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed and flash frozen with liquid N2 before being lysed with lysis buffer containing cycloheximide (Alfa Aesar). Lysate was digested with RNase I (Lucigen ...
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bioRxiv - Molecular Biology 2021Quote: ... the coverslips were washed 3 times with PBS and cells fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Heysham, England) in PBS for 20 minutes ...
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bioRxiv - Biophysics 2020Quote: ... the cells were suspended in 100 μL viscosity-adjusted measurement buffer (0.5% [w/v] methylcellulose [4000 cPs, Alfa Aesar, Germany] in phosphate-buffered saline without Mg2+ and Ca2+ ...
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bioRxiv - Immunology 2020Quote: ... Subsequently the cells were resuspended in TBS buffer (25 mM Tris + 2.7 mM KCl + 137 mM NaCl, pH 7.4; Alfa Aesar / Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed three times with 200μL of FACS buffer and fixed with 200μL of 4% PFA (Alfa Aesar) for 15 mins at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... tubing and pipette tips used to handle wounded cells were treated with 3% Pluronic F-68 (J6608736, Alfa Aesar) in deionized (DI ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated with 10 μM of the blocking agent 7-bromoheptanol (Merrill et al. 2019) (Alfa Aesar, H54762), for 2 h ...
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bioRxiv - Bioengineering 2023Quote: ... the cancer cell lines MCF7 and MDA-MB-231 were fixed with 4% paraformaldehyde (PFA, Alfa Aesar, Haverhill, MA) for 15-30 min at room temperature following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The medium was then replaced by the normal cell culture medium supplemented with 2 mM L-methionine (Alfa Aesar J61904) for 0-3 hr at 37°C for the chase ...
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bioRxiv - Immunology 2020Quote: ... BL21 cells were spun down (3000 RPM, 30 min, 4 °C) and resuspended in Tris-buffered saline (TBS, Alfa Aesar) containing cOmplete™ protease inhibitor cocktail (Sigma Millipore) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with isopropanol (60%) for 5 minutes and then with ORO solution (ORO, 2mg/mL, Alfa Aesar, A12989) for 20 min ...
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bioRxiv - Biophysics 2020Quote: ... Hydrated protein powders were then sealed in aluminum flat neutron cells (sample thickness=0.3 mm) using 1 mm indium wire from Alfa Aesar. Samples were weighed before and after the neutron experiment to verify that they were correctly sealed and no water was lost ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 and BFP expression induction was achieved by treatment of the cells for 3 days with doxycycline (Dox) (1µg/ml) (Hyclate, Alfa Aesar) and single cell clones were seeded by sorting BFP positive cells using the BD FACSAria™ Cell Sorter system ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were stained with alcian blue (1% w/v in 3% v/v acetic acid; Alfa Aesar; goblet cell stain), hematoxylin (0.2% w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Propidium iodide was used as a dead cell marker (1mg/ml aqueous solution; 25535-16-4, Alfa Aesar, Lancashire, UK). Cells were then sorted using fluorescence-activated cell sorting (BD Biosciences ...
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bioRxiv - Systems Biology 2023Quote: ... HeLa cells were transfected with YFP-ATGL variants using Polyethylenimine (PEI, linear, M. W. 20,000 [Alfa Aesar, 9002-98-6]) as transfection reagent (ratio DNA:PEI = 1:5) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in the presence of 100 microliters radio-immunoprecipitation (RIPA) buffer containing protease inhibitors (Alfa Aesar, Stoughton, MA, USA) on ice ...
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bioRxiv - Biophysics 2019Quote: ... cells were suspended in 100 μl of a viscosity-adjusted measurement buffer (0.5% (w/v) methylcellulose (4000 cPs, Alfa Aesar, Germany) in phosphate saline buffer without Mg2+ and Ca2+ ...
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bioRxiv - Microbiology 2021Quote: ... sample volume was adjusted such that approximately 107 cells were harvested and re-suspended in 1 x Hepes Buffered Saline (5X HBS; Alfa Aesar). Cells were washed and resuspended in 1 mL HBS ...
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bioRxiv - Immunology 2019Quote: Immunofluorescence on saponin-permeabilized cells was carried out as described previously [5] except that cells were fixed in methanol-free 4% (w/v) paraformaldehyde (PFA, Alfa Aesar) in phosphate buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2022Quote: Osteogenic differentiation was induced by providing confluent cells with DMEM and 10% FBS supplemented with 10 mM sodium beta-glycerolphosphate (Cat# L03425, Alfa Aesar), 100 nM dexamethasone (Cat# D1756 ...
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bioRxiv - Microbiology 2022Quote: ... to stain the membrane and the cell wall was labeled with 1 μg/ml of an equal mixture of vancomycin (Alfa Aesar) and a BODIPY FL conjugate of vancomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed in the digitonin lysis buffer (1% digitonin [Sigma-Aldrich D141], 137 mM NaCl, 10% w/v glycerol [Alfa Aesar 38988] ...