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Citations for Qiagen :
451 - 500 of 10000+ citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... vIL-6 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) using QuantiTect Multiplex RT-PCR Kits (Qiagen) as described previously (37 ...
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bioRxiv - Cancer Biology 2019Quote: ... First-strand synthesis and RT-PCR were performed using QuantiTect™ Reverse Transcription Kit (Qiagen) and Rotor-Gene™ SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... EGR1 transcripts were converted into cDNA and quantified using a OneStep RT-PCR kit (Qiagen) and a pair of primers (Table S1) ...
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bioRxiv - Physiology 2020Quote: ... cDNA was prepared by reverse transcription using the Quantitect RT-PCR kit (205313, Qiagen, DEU). cDNAs were amplified using assay-on-demand kits (Gpt ...
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bioRxiv - Cell Biology 2020Quote: ... For the RT² Profiler™ PCR Array Human Epithelial to Mesenchymal Transition kit (EMT) (Qiagen), RNA integrity of all the samples was tested by using the Agilent RNA 6000 Nano kit (Agilent Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The harvested contents were subjected to RT-PCR using OneStep Kit (210212, Qiagen, Hilden, Germany). Forward (F ...
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bioRxiv - Microbiology 2020Quote: ... Positive sense vRNA and GAPDH transcripts were measured by qRT-PCR using the QuantiTect SYBR Green RT-PCR kit (Qiagen) on an Applied Biosystems 7300 machine ...
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bioRxiv - Immunology 2019Quote: Total RNA samples were diluted to a concentration of 10 ng/μl prior to gene expression studies by qRT-PCR using QuantiFast SYBR green RT-PCR kit (Qiagen) in 7900HT Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... The remaining 18 μl of RT-PCR DNA was purified by a QIAquick PCR Purification kit (Qiagen, Germantown, MD, USA). Sequences of the purified RT-PCR products were generated using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) following the standard protocol for a QuantStudio 7 Flex RT-PCR system ...
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bioRxiv - Microbiology 2021Quote: ... we used both one-step RT-PCR and two-step (cDNA synthesis followed by separate PCR amplification) reaction designs with commercial One-Step Ahead RT-PCR Kit (Qiagen) or IEH in-house RT ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR mixtures were assembled using 50 ng or 5 µl total RNA and QuantiTect Probe RT-PCR Kit (Qiagen). Amplification and analysis were performed using QuantStudio3 system (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... The PNPLA2 transcript levels in ARPE19 cells determined by quantitative RT-PCR were normalized using the QuantiTect SYBR Green PCR Kit (Qiagen) in the QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... 75 ng of rRNA was reverse transcribed and amplified with 8 PCR cycles using the OneStep RT-PCR kit (Qiagen) and primers MS2_quant_F and MS2_quant_R (Supplemental Table 5) ...
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bioRxiv - Pathology 2020Quote: ... and the RT product was used for detection of miR-574-5p/3p and control RNA Snord68 using the miScript Primer Assay (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: ... MiRs (61 plus 3 controls) were quantified on a custom PCR plate using the miRCURY LNA miR SYBR Green PCR Handbook (Qiagen, Germany). PCR (40 cycles ...
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bioRxiv - Immunology 2021Quote: ... PRRSV-2 RNA was used to amplify cDNA encoding the full-length of N gene by RT-PCR (One-step RT-PCR, QIAGEN) using specific primers with the forward containing a T7 promoter sequence at the 5’ end ...
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bioRxiv - Immunology 2022Quote: ... cDNA was prepared using Quantitect RT- PCR (Qiagen) and RT Q-PCR was performed using the TaqMan Gene Expression Assay (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... cDNA was prepared using Quantitect RT-PCR (Qiagen) and PCR performed with Brilliant III SYBRGreen on a Stratagene Mx3000 ...
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bioRxiv - Cancer Biology 2023Quote: ... using a RT² Profiler PCR Array (Qiagen, 330231), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... level relative to nuclear gene (β actin) by real-time PCR in total DNA isolated from INS1 cells by QIAamp DNA minikit (Qiagen). Rat primers were mt-ND6 ...
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bioRxiv - Biophysics 2021Quote: Thermal stability of CagFbFP and all mutated variants were measured using the Rotor-Gene Q real-time PCR cycler (Qiagen, Germany) with fluorescence excitation at 470 nm and fluorescence detection at 510 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... SYBR I was added to the samples after the reaction was completed and the fluorescence measured in a Real-time PCR machine (Rotor-Gene Q, Qiagen, USA). Triplicates for each conditions were measured and the mean value calculated ...
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bioRxiv - Microbiology 2019Quote: ... Amplification was performed using RotorgeneQ real-time thermocyclers (Qiagen, Australia), with reactions run under the following conditions ...
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bioRxiv - Bioengineering 2019Quote: Genes were quantified by one-step quantitative reverse transcription-polymerase chain reaction (q-PCR) using the QuantiFast SYBR Green RT-PCR kit (Qiagen, UK). A master mix was prepared by mixing 6.25 μl SYBR green ...
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bioRxiv - Physiology 2023Quote: ... Gene expression of 96 fibrosis-associated genes were evaluated using a PCR array kit (RT² Profiler™ PCR Array Rabbit Fibrosis PANZ-120Z, Qiagen) by quantitative real-time PCR using a Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... The QuantiTect SYBR® Green PCR Kit was used for this biomolecular detection (Qiagen, Hilden, Germany).
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bioRxiv - Neuroscience 2021Quote: ... double-digoxigenin locked nucleic acid probe (RNO-MIR-124-3P: CATTCACCGCGTGCCTTA, Tm: 84°C, 339111 YD00614870-BGC, miRCURY LNA™ miRNA Detection Probe, Qiagen) was used at a final concentration of 30 nM ...
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bioRxiv - Microbiology 2020Quote: ... CDNA fragments were amplified with strain-specific primers using the one step RT-PCR kit (Qiagen). Sequencing was performed by Macrogen Europe ...
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bioRxiv - Microbiology 2020Quote: All qPCR and RT-qPCR-reactions were done using the QuantiTect Multiplex PCR NoROX Kit (Qiagen) or QuantiTect Probe RT-PCR Kit (Qiagen ...
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bioRxiv - Systems Biology 2021Quote: ... the presence of RVFV RNA was assessed using the QuantiNova® RT-PCR probe kit (QIAGEN). Samples were tested in duplicate using 5 μl of RNA template per well.
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bioRxiv - Microbiology 2021Quote: ... All reactions were set up in triplicate using a One-Step RT-PCR kit (Qiagen, UK) as per the following run conditions ...
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bioRxiv - Microbiology 2020Quote: ... and miR-42-3p were obtained from the miScript Primer Assays kit (Qiagen). miR-47-3p was used as the endogenous control ...
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bioRxiv - Cancer Biology 2020Quote: ... Digoxigenin labeled miRCURY LNA probes (HSA-MIR-10B-5P, SCRAMBLE-MIR, miRCURY LNA U6, Qiagen) were diluted in hybridiziation buffer after a denaturation step at 90 °C and incubated on cells for 30 min (60 min tissue sections ...
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bioRxiv - Genetics 2022Quote: ... PCR product was pooled and purified two times with a QIAquick PCR Purification Kit (Qiagen, Inc., Valencia, CA, USA) and DNA quantity was measured on an Agilent 2200 TapeStation (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized by Qiagen Omniscript RT Kit (#205111, Qiagen). RT-qPCR was performed using QuantiTect SYBR® Green PCR Kit (#204145 ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized by Qiagen Omniscript RT Kit (#205111, Qiagen). RT-qPCR was performed by using QuantiTect SYBR® Green PCR Kit (#204145 ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized by Qiagen Omniscript RT Kit (205111, Qiagen). The relative mRNA level of indicated genes was normalized to that of the internal control Hsp90 and calculated by the equation 2^(Ct(cycle threshold ...
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bioRxiv - Bioengineering 2019Quote: ... Sensiscript RT Kit (QIAGEN, Germany) was used for cDNA synthesis with 50 ng per reaction according to the manufacturers’ instructions ...
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bioRxiv - Genetics 2022Quote: ... miScript II RT Kit (Qiagen) was used to synthesize cDNA ...
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bioRxiv - Microbiology 2022Quote: ... 2 μL of fecal DNA from each mouse was used as template in 25 μL total reactions of SYBR Green Real Time PCR Master Mix as instructed by the manufacturer (Qiagen; Hilden, Germany). Reactions were carried using CFX Connect Real Time PCR Detection System (BioRad ...
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bioRxiv - Immunology 2022Quote: ... qPCR was performed using the Mx3000/Mx3005P Real-Time PCR System. All primers were used in this study were ordered from Qiagen BCAM (PPH12956A), SCGB1A1 (PPH01032F) ...
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Integrative analysis of hexaploid wheat roots identifies signature components during iron starvationbioRxiv - Plant Biology 2019Quote: ... qRT-PCR was performed using QuantiTect SYBR Green RT-PCR mastermix (Qiagen, USA) with programs recommended by the manufacturer in the ABI 7700 sequence detector (Applied Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-PCR was performed in triplicate using SYBR Green PCR Master Mix (Qiagen) and run on Viia7 platform (Applied Biosystems) ...
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bioRxiv - Physiology 2021Quote: ... reaction tubes were transferred to a Rotor-Gene Q PCR thermal cycler for product amplification using a one-step protocol (QuantiFast SYBR® Green RT-PCR Kit, Qiagen, UK). The amplification protocol was as follows ...
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bioRxiv - Neuroscience 2021Quote: ... pmirGLO-3’UTR_FosB and pmirGLO-3’UTR_Npas4 plasmids were purchased from Creative Biogene and the mimick-miR-1a-3p and mimick-miR-negative control from Qiagen (miScript miRNA Mimics). The protocol used is described in DOI ...
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bioRxiv - Neuroscience 2022Quote: ... miR-495 pLNA (miRCURY LNA miRNA Power Inhibitor HSA-MIR-495-3P, Qiagen #339130 YI04101229-DCA).
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bioRxiv - Neuroscience 2022Quote: ... miR-329 pLNA (miRCURY LNA miRNA Power Inhibitor RNO-MIR-329-3P, Qiagen # 339130 YI04101481-DCA), miR-495 pLNA (miRCURY LNA miRNA Power Inhibitor HSA-MIR-495-3P ...
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bioRxiv - Immunology 2021Quote: ... RT² Profiler PCR Array (QIAGEN, Cat. no. PAMM-021Z) was used ...
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bioRxiv - Microbiology 2021Quote: ... 10 μl 5x One-step RT-PCR buffer (Qiagen), 10 μl Triton-x100 (0.3%) ...