Labshake search
Citations for Qiagen :
351 - 400 of 5854 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... RT-PCR was performed using QIAGEN’s RT2 Profiler™ PCR Array Human Cell Death PathwayFinder (QIAGEN, Hilden, Germany). RT-PCR data was analyzed and visualized for pathway analysis by GeneSpring GX 14.9 (Agilent Technologies ...
-
bioRxiv - Bioengineering 2023Quote: Isolated miRNA content was quantified by real time quantitative PCR (RT-qPCR) using the miScript PCR Kit (Qiagen) with a CFX Connect Real-Time system (Bio-Rad) ...
-
bioRxiv - Plant Biology 2023Quote: Real-Time Quantitative PCR (RT-qPCR) was performed with the Rotor-Gene SYBR® Green PCR Kit (Qiagen), on a Rotor Gene-Q(R ...
-
bioRxiv - Cancer Biology 2020Quote: ... at a final concentration of 50 nM and confirmed by RT-qPCR with the miRCURY LNA™ Universal RT microRNA PCR system (Qiagen) and a StepOnePlus thermocycler (Applied Biosystems) ...
-
bioRxiv - Physiology 2023Quote: ... A one-step real-time quantitative polymerase chain reaction (RT-qPCR) was performed using a QuantiFast SYBR Green RT-PCR one-step kit on a Rotorgene 3000Q thermocycler (Qiagen, UK). Each reaction was setup as follows ...
-
bioRxiv - Genomics 2021Quote: ... Synthetic cel-miR-39 mimic (miScript miRNA Mimics, QIAGEN) was spiked to each RNA sample at a ratio of 11 fmol per ug of total RNA and used as an exogenous control for normalization ...
-
bioRxiv - Cancer Biology 2020Quote: ... Ct values for hsa-miR-145-5p (Qiagen, YP00204483) were normalized to expression levels of hsa-miR-423-5p (Qiagen ...
-
bioRxiv - Cell Biology 2022Quote: ... Syn-cel-miR-39 spike in synthetic RNA (Qiagen) was added to monitor extraction efficiency ...
-
bioRxiv - Molecular Biology 2021Quote: ... PCR and sequencing primers were designed using the PyroMark Assay Design software (Qiagen). All successfully amplified PCR products were analysed on a PyroMark Q24 (Qiagen) ...
-
bioRxiv - Systems Biology 2019Quote: ... with custom miRCURY LNA PCR primers (Qiagen cat. no. 339306, Supplementary Table 7). Each 10 µL reaction volume contained 5 µL 2x miRCURY SYBR Green Master Mix ...
-
bioRxiv - Biophysics 2020Quote: ... Real-Time PCR was performed with specific primers purchased from Qiagen (Tables 1,2) and Power SYBR Green PCR Master Mix (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2022Quote: ... Quantitative real time PCR was performed using SYBR Green with QuantiTect Primers (Qiagen) and using ppia gene as a reference gene ...
-
bioRxiv - Developmental Biology 2019Quote: ... PCR and sequencing primers were designed by Pyromark Assay Design Software 2.0 (Qiagen) and available upon request ...
-
bioRxiv - Genetics 2020Quote: ... Multiplex polymerase chain reaction (PCR) was performed using three primer pairs and the Multiplex PCR Kit (QIAGEN, Hilden, Germany). Each reaction contained 2× QIAGEN multiplex PCR master mix ...
-
bioRxiv - Cancer Biology 2021Quote: ... For evaluation of correlation with ALDH1A1 and ALDH1A3 the RT2 profiler PCR Array gene sets (Qiagen) were used ...
-
bioRxiv - Molecular Biology 2021Quote: ... All experiments were done with Qiagen OneStep RT-PCR reagents (Qiagen, Hilden, Germany) on a MIC thermocycler (BMS ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... RT-qPCR was performed in SYBR Green PCR Master Mix (QIAGEN, California, USA) on CFX-96 system ...
-
bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed using the miScript SYBR Green PCR Kit (QIAGEN, 218073) on a step-one plus PCR machine (Applied Biosystems) ...
-
bioRxiv - Biochemistry 2020Quote: ... and QIAGEN® OneStep RT-PCR Kit were purchased from QIAGEN (Frederick, MD). RiboMAX™ Large Scale RNA Production Systems-T7 was purchased from Sigma-Aldrich (St ...
-
bioRxiv - Neuroscience 2021Quote: ... RT-qPCR was performed using QuantiTect SYBR® Green PCR Kit (#204145, QIAGEN) on Agilent MP3005P thermocycler ...
-
bioRxiv - Microbiology 2021Quote: ... the QIAcuity One-Step Viral RT-PCR Kit (Cat No. 1123145, Qiagen, Germany) and the 24-well 26K Nanoplates (Cat No ...
-
bioRxiv - Plant Biology 2020Quote: ... Initial amplification was carried out using a One-Step RT-PCR Kit (QIAGEN) in a BIO-RAD T100 Thermal Cycler ...
-
bioRxiv - Microbiology 2021Quote: ... qPCR analyses were performed using QuantiFast SYBR Green RT-PCR Kit (Qiagen, 204154) on StepOne Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Neuroscience 2020Quote: ... and for the RT-PCR reaction—RT2 SYBR Green ROX qPCR Matermix (Qiagen). The average Ct values across the three housekeeping genes B2m ...
-
bioRxiv - Cancer Biology 2019Quote: ... RT-qPCR was performed subsequently using a QuantiTect SYBR Green PCR Kit (Qiagen). We purchased all primers from Qiagen ...
-
bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was performed using a Quanti-Tect SYBR green PCR kit (Qiagen) and primers for the target genes (Table S2) ...
-
bioRxiv - Cell Biology 2022Quote: ... its expression level was quantified using QuantiFast Pathogen RT-PCR Kit (Qiagen, #211352) and 2019-nCoV RUO Kit (IDT ...
-
bioRxiv - Microbiology 2020Quote: ... Gene segments and DVGs were amplified using a OneStep RT-PCR kit (Qiagen), using the isolated serial passage RNA as a template and gene-specific primers that bind the 5′ and 3′ RNA termini (Table 1) ...
-
bioRxiv - Cell Biology 2019Quote: ... Quantitative RT-qPCR was performed using QuantiTect SYBR®-Green PCR kits (Qiagen) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
-
bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was conducted by QuantiTect SYBR® Green PCR Kit(204145, QIAGEN) approaches on Agilent MP3005P thermocycler ...
-
bioRxiv - Molecular Biology 2021Quote: ... RT-qPCR was performed using a QuantiTect SYBR® Green PCR kit (Qiagen) and primers for the target genes (Table 2) ...
-
bioRxiv - Microbiology 2020Quote: ... Viral RNA was analyzed using the QuantiTect SYBR Green RT-PCR kit (Qiagen) in a LightCycler 480 (Roche ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... QuantiTect Probe RT-PCR (reverse transcription polymerase chain reaction) Kit (QIAGEN, Hilden, Germany) was used to perform RRT-PCR reactions in the ABI 7300 Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Bioengineering 2023Quote: ... and analyzed using the RT² Profiler PCR Data Analysis Tool on GeneGlobe (Qiagen). For reverse transcription ...
-
bioRxiv - Biophysics 2023Quote: ... We assembled qPCR reactions with QuantiFast SYBR Green RT-PCR Kit (Qiagen # 204156), gene-specific oligonucleotides (Supplementary Table 14 ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... Expression of AmTyr1 was quantified using QuantiFAST SYBR Green RT-PCR kit (QIAGEN) on Applied Biosystem 7900 cycler (ASU DNA Facilities ...
-
bioRxiv - Neuroscience 2020Quote: ... PCR assays were performed based on previous published method (Gonatopoulos-Pournatzis et al., 2018) OneStep RT-PCR kit (QIAGEN) was used according to the manufacturer’s recommendations ...
-
bioRxiv - Genomics 2022Quote: ... cDNA of each gene target was reverse-transcribed and PCR amplified with the PyroMark OneStep RT-PCR kit (QIAGEN) using one biotinylated (reverse ...
-
bioRxiv - Cancer Biology 2019Quote: ... Real time one step qRT-PCR was carried out using the QuantiTect SYBR® Green RT-PCR Kit (Qiagen) according to manufacturer’s instructions before analysis on the 7900 PCR machine (Applied Biosystems) ...
-
bioRxiv - Microbiology 2019Quote: ... qRT-PCR to quantify cap and gyr mRNA was performed using the QuantiFast SYBR-Green RT-PCR kit (Qiagen). Standard curves were generated using 10-fold serial dilutions (104 to 108 copies ...
-
bioRxiv - Microbiology 2023Quote: ... SYBR green real-time PCR assay was carried out in a 20μL PCR mixture volume consisting of 10 μL of 2X Quantitect SYBR green RT-PCR Master Mix (Qiagen) containing HotStarTaq DNA polymerase ...
-
bioRxiv - Microbiology 2020Quote: The uniplex RT-qPCR assays to detect DENV were performed with the QuantiTect Probe RT-PCR Kit (Cat. 204445, Qiagen, Hilden, Germany), and the amplification of RT-PCR was performed with Superscript III Platinum One-Step reagents RT-qPCR System (Cat ...
-
bioRxiv - Molecular Biology 2021Quote: ... 40 ng of each sample was used per reverse transcription-quantitative polymerase chain reaction (RT-qPCR) reaction using the QuantiTect SYBR® Green RT-PCR Kit (Qiagen, 204243) on Rotor-Gene Q (Qiagen ...
-
bioRxiv - Molecular Biology 2021Quote: ... Oligonucleotides containing shRNA inserts were PCR-amplified with primers 5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’ and 5’-TGAACTCGAGAAGGTATATTGCTGTTGACAGTGAGCG-3’ and purified with QIAquick PCR Purification Kit (Qiagen). shRNA inserts and miRE18_LT3GEPIR_Ren714 backbone (inducible via Tet-On system ...
-
bioRxiv - Genetics 2020Quote: ... Analyses of HTT CAG repeat size in both HttQ111 mice and in patient fibroblasts was performed by PCR using human-specific HTT primers and Taq PCR Core Kit with Q solution (Qiagen), as previously described (17 ...
-
bioRxiv - Immunology 2021Quote: ... Each AES was then amplified by PCR on a 3 kb fragment using primer X5 and X6 and purified using QIAquick PCR Purification kit (Qiagen). For gene yrbE4A ...
-
bioRxiv - Microbiology 2020Quote: ... templates containing a T7 phage promoter sequence (5’-TAATACGACTCACTATAGGG-3’) were amplified by PCR using corresponding primer pairs and were purified with a QIAquick PCR purification kit (Qiagen). The primers used are listed in S1 Table ...
-
bioRxiv - Molecular Biology 2022Quote: ... We designed species specific MALAT1 tiling primers for human and green monkey (Table S1) and performed multiplex PCRs following Qiagen Multiplex PCR protocol (Qiagen). cDNA was divided equally between primer sets ...
-
bioRxiv - Cancer Biology 2023Quote: ... Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen, Germany). 2-nd PCR (PCR 2 ...
-
bioRxiv - Immunology 2023Quote: ... The first round of PCR was carried out using the ImmunoSEQ proprietary PCR primer mix (32 μL per sample containing 25 uL of QIAGEN 2× Multiplex PCR Master Mix ...