Labshake search
Citations for Qiagen :
451 - 500 of 5860 citations for rno mir 125b 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2019Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
-
bioRxiv - Physiology 2019Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
-
bioRxiv - Neuroscience 2019Quote: ... All the qPCR experiments were performed using Quantifast SYBR Green RT-PCR Kit (Qiagen) in a LightCycler 480 II System (Roche) ...
-
bioRxiv - Immunology 2019Quote: ... Human RAC2 3’UTR was amplified with One Step Ahead RT-PCR kit (Qiagen) from human mRNA using the following primers RAC2 3’UTR+ ...
-
bioRxiv - Cancer Biology 2021Quote: ... One-step qRT-PCR reactions were performed in triplicate using QuantiTech RT mix (QIAGEN) on the Bio-Rad C1000 Thermocycler and CFX96 system (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed by using QuantiTect SYBR® Green PCR Kit (#204145, QIAGEN) on Agilent MP3005P thermocycler ...
-
bioRxiv - Physiology 2020Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription was initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
-
bioRxiv - Microbiology 2019Quote: ... For this purpose we used the QIAGEN OneStep RT-PCR Kit (Qiagen, CA-EUA) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Ig Vκ and Vλ from positive wells were amplified by RT-PCR (210210; QIAGEN) and nested PCR (203205 ...
-
bioRxiv - Physiology 2020Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription was initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
-
bioRxiv - Molecular Biology 2021Quote: ... TER1 was amplified from circularized RNA with the OneStep RT-PCR Kit (Qiagen, 210210) (primer sequences in Table S6) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time RT-PCR was performed on a Rotor-Gene Q instrument (Qiagen) using SYBR Premix Ex-Taq II (TakaraBio) ...
-
bioRxiv - Microbiology 2020Quote: ... HIV-1 RNA was analyzed using the QuantiTect SYBR Green RT-PCR kit (Qiagen) in a LightCycler 480 (Roche) ...
-
bioRxiv - Immunology 2021Quote: ... Viral load was quantified by Taq-Man qPCR using OneStep RT-PCR Kit (Qiagen) and AriaMx Real-time PCR Systems (Agilent) ...
-
bioRxiv - Neuroscience 2020Quote: ... RNA (500 ng) was reverse transcribed into cDNA using QuantiTect RT-PCR kit (Qiagen). RT-PCR was performed on a BioRad CFX384 thermocycler using pre-validated Taqman primers (Applied Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: ... RT-qPCR mix was prepared using the miRCURY LNA SYBR Green PCR kit (Qiagen). qPCR was performed using the QuantStudio 7 Flex System ...
-
bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in triplicate using the QuantiNova SYBR Green PCR Kit (Qiagen) and the following primer sequences ...
-
bioRxiv - Physiology 2023Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Each sample was analysed in duplicate ...
-
bioRxiv - Developmental Biology 2023Quote: ... and RT-qPCR was performed using the miScript SYBR Green PCR Kit (Qiagen, 218073) together with the miRCURY LNA miRNA PCR Assay (Qiagen ...
-
bioRxiv - Neuroscience 2024Quote: ... RT-PCR was normalized using the housekeeping genes hypoxanthine phosphoribosyl-transferase 1 (HPRT1, Qiagen QuantiTect Primer Assay ...
-
DNA and RNA-SIP reveal Nitrospira spp. as key drivers of nitrification in groundwater-fed biofiltersbioRxiv - Physiology 2019Quote: ... and from sacrificed column experiments and full scale biofilters were reverse transcribed using reverse primer 1492R with Sensiscript RT kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Three micrograms of RNA were used to synthesize cDNA with the Omniscript RT kit using oligo dT primers (Qiagen 205113). Resulting cDNA was diluted 1:2 and used for real-time PCR with Power SYBR Green (Thermo Fisher Scientific 4367659 ...
-
bioRxiv - Molecular Biology 2021Quote: The gene of interest was amplified using PCR with gene-specific primers and purified using the QIAquick PCR Purification Kit (QIAGEN, Germany). Gene-specific T7 promoter sequences were added to the 5’ and 3’ end of the purified product using PCR and were purified ...
-
bioRxiv - Molecular Biology 2022Quote: ... The qRT-PCR was performed using 20 miRNA primers (Supplementary Table 1) and miScript SYBR Green PCR Kit (Qiagen, Hilden, Germany). RNA U6 was used as the endogenous control ...
-
bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were performed for all primers in 10μl reaction containing 4μl Qiagen multiplex PCR buffer mix (QIAGEN Inc., Hilden, Germany), 0.3 μM primer mix for each set ...
-
bioRxiv - Biophysics 2019Quote: ... and DNase Set (QIAGEN). Gli1 and Hprt levels were determined by one-step quantitative reverse transcriptase PCR (qRT–PCR ...
-
bioRxiv - Bioengineering 2019Quote: Genes were quantified by one-step quantitative reverse transcription-polymerase chain reaction (q-PCR) using the QuantiFast SYBR Green RT-PCR kit (Qiagen, UK). A master mix was prepared by mixing 6.25 μl SYBR green ...
-
bioRxiv - Physiology 2023Quote: ... Gene expression of 96 fibrosis-associated genes were evaluated using a PCR array kit (RT² Profiler™ PCR Array Rabbit Fibrosis PANZ-120Z, Qiagen) by quantitative real-time PCR using a Thermal Cycler (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2020Quote: ... with all primers listed in Supplementary Table 1 and then purified (QIAGEN, PCR purification kit). Before nick translation ...
-
bioRxiv - Cancer Biology 2022Quote: ... Quantitative real time PCR was performed using SYBR Green and the following primers from Qiagen: Ppia (QT00247709) ...
-
bioRxiv - Immunology 2020Quote: ... 0.125 μl 20 μM SmarterR reverse primer (30) and 1 μl PCR-grade H2O (Qiagen). The amplification was performed in a thermal cycler (lid temperature 98°C ...
-
bioRxiv - Genetics 2019Quote: ... genomic regions were PCR amplified using sequence specific primers with HotStarTaq Polymerase Kit (Qiagen # 203203). PCR primer sequences used to amplify the CASK_SS variant ...
-
bioRxiv - Cancer Biology 2021Quote: ... we pre-coated 384-well plates with a mixture containing the PCR primer mix (Qiagen; sequences and category numbers available through online data repository (17)) ...
-
bioRxiv - Neuroscience 2023Quote: ... PCR primers were designed using the PyroMark Assay Design 2.0 software (Qiagen, Supplementary Table 2). The assay for MBP was tested for its sensitivity using the EpiTect PCR Control DNA Set (Qiagen) ...
-
bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed with 9ng of cDNA per reaction using Quantitect primers (Qiagen).
-
bioRxiv - Immunology 2024Quote: ... using primers (Supplementary Table 2) and purified the products using QIAquick PCR Purification Kit (Qiagen). PCR-products were Sanger sequenced and knockout scores were determined using the ICE analysis tool (https://ice ...
-
bioRxiv - Genetics 2021Quote: ... PCRs were set-up in 30 μl reactions using three μl of 10x HotStarTaq buffer (Qiagen, Hilden Germany), 2.4 μl of 10 mM d’NTPs (Fisher Scientific ...
-
bioRxiv - Plant Biology 2021Quote: ... Sets of 95 ligations were pooled into one sample and purified using QIAquick PCR Purification Kit (Qiagen, Germany). The pooled ligation mixtures (5 μL ...
-
bioRxiv - Microbiology 2019Quote: miR-21 inhibitor and its negative control were purchased from Qiagen. Cells were seeded in six-well plates and transfected with miR-21-inhibitor or inhibitor-control using Lipofectamine 2000 (Invitrogen ...
-
bioRxiv - Developmental Biology 2021Quote: ... miR-let7 levels were analyzed using the miScript protocol (Qiagen, 218193) using primers amplifying miR let7 (Qiagen ...
-
bioRxiv - Developmental Biology 2022Quote: ... miRCURY ™LNA microRNA inhibitor (anti-miR-223) (Qiagen, Hilden, Germany) is the sequence-specific and chemically modified oligonucleotide that specifically target and knockdown miR-223 miRNA molecules.
-
bioRxiv - Physiology 2022Quote: ... 0.002 fmol of the synthetic miRNA cel-miR-39-3p (Qiagen) and 0.15 fmol of the spike-in UniSp6 (Qiagen ...
-
bioRxiv - Immunology 2021Quote: ... Primers were from QuantiTect Primer Assays (Qiagen). Expression was normalized to mouse or human Gapdh.
-
bioRxiv - Microbiology 2022Quote: ... Primers were from QuantiTect Primer Assays (Qiagen).
-
bioRxiv - Cancer Biology 2021Quote: ... Amplification of cDNA for validation of doxycycline induction was performed with U2AF1 primers (forward: 5’-GGCACCGAGAAAGACAAAGT-3’; reverse: 5’-CTCTGGAAATGGGCTTCAAA-3’) and PCR products were purified using QIAquick PCR purification kit (QIAGEN, Cat #28104) before Sanger sequencing ...
-
bioRxiv - Cell Biology 2023Quote: ... We obtained single amplified products for12 out of the 14 PCR primer pairs and these amplified products were purified using the QIAquick PCR Purification Kit (28104, Qiagen, Germantown, MD) before sequencing ...
-
bioRxiv - Biochemistry 2020Quote: Reverse transcription reactions containing 2μl of purified RNA and 2μl of 10mM HNqPCRrev1 primer in a final volume of 20μl were performed using the Qiagen Sensiscript RT kit (Qiagen, catalog # 205211) per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... All RNAs for the miRNA investigation were transcribed using miScript RT kits and subsequent qPCR performed using miScript Primer Assays and miScript SYBR kits (Qiagen, Courtaboeuf). Experiments were performed with at least triplicates for each data point ...
-
bioRxiv - Cancer Biology 2021Quote: RT-PCR was performed using the SYBR green master mix (RealQ plus 2X, Qiagen-Germany). Amplification was done by Exicycler96 (Bioneer-S ...
-
bioRxiv - Microbiology 2020Quote: ... vIL-6 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) using QuantiTect Multiplex RT-PCR Kits (Qiagen) as described previously (37 ...