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Citations for Qiagen :
1 - 50 of 65 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 150 µL RLT buffer (Qiagen) with 1:10 β-mercaptoethanol (55mM ...
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bioRxiv - Immunology 2021Quote: ... 150 μl Trizol (Qiagen, Hilden, Germany) were added onto the upper surface of epithelial culture ...
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bioRxiv - Molecular Biology 2024Quote: ... with 150 µg/mL RNAse A (Qiagen) overnight at 4 °C in the dark ...
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bioRxiv - Microbiology 2023Quote: ... and 150 μL of Proteinase K (Qiagen, #19131), vortexed ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNaseA (150 μg/ml, final concentration; Qiagen) and incubated with Streptavidin Sepharose High Performance (Cytiva ...
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bioRxiv - Cancer Biology 2020Quote: ... 150 μl bed volume Ni-NTA agarose (QIAGEN, Venlo, Netherlands, 30210) was added to the samples and rotated for two hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant incubated with 150 μL Ni-NTA affinity resin (QIAGEN) on the nutator at 4°C for 3 h ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was eluted by incubating in 150 μl PKD buffer (Qiagen) with 10 μl of Proteinase K (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... Each DNA pellet was resuspended in 150 µL elution buffer (Qiagen, # 1014819).
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bioRxiv - Biochemistry 2022Quote: ... 150 mM NaCl) and applied to pre-equilibrated Ni-NTA resin (Qiagen). Column was washed in at least 30 volumes of wash buffer ...
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bioRxiv - Genomics 2020Quote: ... and from blood (150 µl) with the QIAamp DNA Blood Mini Kit (Qiagen). DNA concentration was measured using the NanoDrop™ 2000 spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Neuroscience 2024Quote: ... Size selection was performed via agarose gel (150-1000 bp) followed by Qiagen MinElute gel extraction according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Size selection was performed via agarose gel (150-1000 bp) followed by Qiagen MinElute gel extraction according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 150 ng of CRD-STREX-GFP plasmid was diluted in proprietary EC buffer (Qiagen) and enhancer was added to it at 1:125 (μl/ng DNA ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted in 150 μl phenol-chloroform and purified on a QIAquick column (Qiagen) and eluted in 50 μl EB buffer (10 mM Tris-HCl pH 8.5 ...
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bioRxiv - Immunology 2022Quote: ... then 150 µL of homogenate was added to an equal volume of buffer RLT (Qiagen) for RNA extraction ...
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bioRxiv - Physiology 2020Quote: ... RNA from human islets (∼150 for each donor) was extracted with RNeasy Mini Kit (Qiagen) and was reverse transcribed using the High Capacity Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from 150-200 islets using the RNeasy Micro kit (Qiagen, Valencia, CA). RNA was quantified by spectrophotometry with a NanoDrop 2000 (Life Technologies Inc.) ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments <150 bp in length were removed using the GeneRead Size Selection Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA (150 ng) was used for cDNA synthesis using the Omniscript RT Kit (Qiagen). Quantitative real-time PCR was performed on a QuantStudio 6 Pro real-time PCR system and Taqman Universal PCR Master Mix according to the manufacturer instructions ...
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bioRxiv - Genetics 2021Quote: Dissected larval or adult guts were homogenised in 150 μL of QIAzol Lysis Reagent (QIAGEN, 79306), and stored at −80 °C ...
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bioRxiv - Genetics 2021Quote: Pools of 150 flies per sample were homogenized using a TissueLyser (Qiagen, Inc., Germantown, MD, USA). Genomic DNA was extracted with the Gentra Puregene Tissue kit (Qiagen Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The remaining 150 μL of RLT-dissolved embryo were used for RNA extraction (QIAGEN RNeasy Kit), the RNA was quantified and equal amounts were used for reverse transcription ...
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bioRxiv - Plant Biology 2020Quote: ... Paired sequence reads of 150 bp were aligned using CLC Genomics Workbench 10.0.2 (QIAGEN, Aarhus, Denmark). The Arabidopsis genome assembly of ftp.ensemblgenomes.org/pub/current/plants/fasta/arabidopsis_thaliana/dna/Arabidopsis_thaliana.TAIR10.dna.toplevel.fa.gz (downloaded 10.8.2019 ...
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bioRxiv - Physiology 2022Quote: ... approximately 150-200 mg of snap frozen placenta tissue was lysed in RLT-lysis buffer (Qiagen) with homogenization aided by a tissue-lyzer ...
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bioRxiv - Microbiology 2024Quote: ... 150 μl of the supernatant was extracted with a DNeasy blood & tissue kit (Qiagen, Maryland, USA), and were extracted as purified DNA in 50 μl of distilled water ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The sections were hybridized with hsa-miR-544a miRCURY LNA miRNA Detection Probe (150 nM; Qiagen), U6 snRNA (2 nM-positive control ...
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bioRxiv - Cell Biology 2020Quote: Adrenal glands were homogenized in ammonium-bicarbonate buffer (150 mM ammonium bicarbonate, pH 7) with TissueLyser (Qiagen). Protein content was assessed using BCA Protein Assay Kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 μL of supernatant were removed for RNA extraction with the QIAamp Viral RNA Kit (Qiagen, Germany). Midguts and saliva were extracted directly with the same kit after complete digestion in lysis buffer for 30 min at room temperature (about 25 °C) ...
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bioRxiv - Plant Biology 2024Quote: ... the fragment around 150 bp were cut from gel and cleaned with QIAquick Gel Extraction Kit (QIAGEN). The libraries were sequenced using 50bp single reads on an Illumina Hiseq 2500.
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bioRxiv - Microbiology 2020Quote: RNA extraction was performed from 150 µl of ED cell supernatant using the QIAamp viral RNA kit (Qiagen). EAV genome copy number was determined by one step RT-qPCR using the QuantiTect™ Virus + ROX Vial Kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... 150 μL of the aggregated or non-aggregated cells were then washed with 2 volumes of RNAprotect (Qiagen) to prevent RNA degradation ...
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bioRxiv - Developmental Biology 2021Quote: ... and fragments sized 150-350 bp were excised and then purified using a MinElute Gel Extraction kit (QIAGEN 28604). The purified fragments were treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (Zymo Research D5005) ...
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bioRxiv - Biochemistry 2022Quote: ... The remaining recovery was grown overnight in 150 mL LB + chloramphenicol and plasmid DNA was isolated by MaxiPrep (Qiagen). The complexity of the SpCas9 catalytic and PAM domain libraries were estimated to be 77,400 and 292,600 respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... Wing imaginal discs or their neoplasms were thus obtained were resuspended in 150-300 μL of RLT Buffer (Qiagen) containing 1.5-3 μL of ◻-mercaptoethanol ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was lysed with a lysis buffer (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, 20 mM EDTA, 1% SDS) and proteinase K (Qiagen) was added to samples to degrade proteins ...
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bioRxiv - Immunology 2023Quote: ... The periplasmic fraction was filtered with a 0.22 um filter and incubated with 4 mL 50% Ni-NTA resin equilibrated in wash buffer (20 mM HEPES, pH 7.5, 150 mM NaCl, 40 mM imidazole) (Qiagen) per liter of initial bacterial culture ...
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bioRxiv - Microbiology 2024Quote: ... and 150-175 cm depth increments were collected and extracted for DNA using the MoBio PowerSoil Pro Kit (QIAGEN) and resuspended in 50 μL of 10 mM Tris buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 ul of lysate was used for the total RNA preparation with the RNeasy kit (Qiagen, cat. No. 74106). The remaining lysate was used for treatment with RNaseI (100 Unit per 120µg of lysate ...
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bioRxiv - Genomics 2024Quote: ... DNA extractions were performed by combining 150 µl of demineralized material with 1.5 ml binding buffer (500 ml Qiagen PB ...
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bioRxiv - Cancer Biology 2024Quote: ... were mapped to mouse genome NCBI37/mm9 reference to achieve an average depth coverage above 150× for the targeted exon regions by using CLC Genomics Workbench v11.0 (CLCbio, Qiagen). The single nucleotide variants and short indels were then called using the Variant Detection tool ...
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bioRxiv - Neuroscience 2021Quote: ... To each of those mixtures 150 μl of ice-cold MeOH was added and subsequently homogenized using a TissueLyser II (Qiagen). To support protein precipitation ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
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bioRxiv - Genetics 2021Quote: ... and 99F8 (150 ng pU6-3-99F8-gRNA (DGRC Cat# 1549) and 150 ng pBS-99F8-5’HR-attP>>Act5C::GFP<
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bioRxiv - Genomics 2021Quote: ... gel was viewed and cut between 150-500bp and DNA was extracted from gel pieces using QIAquick Gel Extraction Kit (QIAGEN). Libraries were normalized using the KAPA Library Quantification Kit (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products with sizes from 150 bp to 300 bp were extracted from 2% agarose gel using MinElute Gel Extraction Kit (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... SUMO protease was added at a final concentration 1/10 and the mixture was dialyzed overnight at 4 °C against the buffer DB6 (50 mM Tris-HCl pH 8, 150 mM NaCl, 10 mM imidazole) and applied on a NiNTA column (Qiagen) equilibrated in the DB6 buffer ...
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bioRxiv - Microbiology 2023Quote: ... Then 4 μl RNase A (100 mg/ml) were added to the tubes followed by 150 μl of buffer AL (lysis buffer, Qiagen). After mixing by vortexing ...
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bioRxiv - Microbiology 2023Quote: ... 150 mg of soil were weighed to carry out the DNA extraction using the DNeasy PowerSoil kit (Qiagen, Toronto, ON) following the manufacturer’s protocol with the following modification ...
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bioRxiv - Genomics 2023Quote: ... Run time was set to 10 minutes and DNA between 150 and 500 bp was excised from the gel and purified using the QIAquick Gel Extraction Kit (Cat. #28706X4, Qiagen) according to the manufacturer’s instructions ...