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Citations for Qiagen :
301 - 339 of 339 citations for hnRNP K since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Samples were treated with RNase A and Proteinase K for post-immunoprecipitation and then the DNA was purified using QIAquick PCR Purification Kit (Qiagen, 28104). ChIP primers sequences can be found in Supplemental Table 3 ...
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bioRxiv - Physiology 2020Quote: ... in lysis buffer (180 µl buffer ATL with 20 µl proteinase K) provided in the DNeasy spin column kit (Qiagen, UK) using a Roche Magnalyser instrument and homogenization tubes containing ceramic beads (Roche ...
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bioRxiv - Microbiology 2020Quote: ... and then with (2) 15 µl Proteinase K [>600 mA/ml] and 5 µl Rnase A [100 mg/ml] (Qiagen, Germany) for 5min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... the beads were transferred to a new 0.5ml tube and incubated in 100 μL ChIP elution buffer (10mM Tris-HCl pH8.0, 5mM EDTA, 300mM NaCl, 0.5% SDS) containing 5 µL proteinase K (Qiagen, 20mg/ml stock) at 55°C for 2 h and then at 65°C for 4 h ...
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bioRxiv - Physiology 2023Quote: ... in lysis buffer (180 µl buffer ATL with 20 µl proteinase K) provided in the DNeasy spin column kit (Qiagen, UK) using a Roche Magnalyser instrument and homogenization tubes containing ceramic beads (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... RNA extraction was performed using the RNeasy midi kit with proteinase K digestion and on-column DNase digestion according to the manufacturer’s instructions (Qiagen, Hilden, Germany). The samples were then sent to Admera Health for quality control ...
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bioRxiv - Neuroscience 2023Quote: ... After protein digestion (PK Buffer and Proteinase K (20 mg/mL) for 2 hrs at 45°C) DNA was purified with QIAquick PCR Purification Kit (Qiagen, Germany) according to manufacturer instruction ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted DNA by adding 3-5 mg of tail tissue to 150 µl of 10% ChelexR resin (Bio- Rad, Inc.) with 1.4 µl of Proteinase K (20 mg/ml, Qiagen, Chatsworth, CA), incubating at 55°C for 180 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following an incubation at 55°C for 1 hr in 450 µl of lysis buffer with proteinase K (QIAGEN, Hilden, Germany). Each colony was genotyped using twelve microsatellite loci69 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were digested with proteinase K overnight and DNA was extracted the next day by QIAamp DNA Mini Kit (ref 51306) (Qiagen, USA) with washes performed twice ...
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bioRxiv - Physiology 2023Quote: ... 0.5% SDS and 20mg/ml of Proteinase K) and DNA purified using the DNeasy Blood and Tissue Kit (Qiagen, Venlo, Netherlands). PCR was performed with extracted DNA and primers for the recombined and non-recombined alleles as previously described [8] ...
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bioRxiv - Cancer Biology 2023Quote: ... Deproteination of the D-2-HG quantification aliquot was achieved using 3.0 mL of Proteinase K (Qiagen, Catalog #19131, Hildren, Germany) per 100.0 mL of cell lysis solution ...
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bioRxiv - Genetics 2024Quote: ... Total genomic DNA was subsequently isolated from the ATL/Proteinase K mixture with the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was further treated with 25 µL of proteinase K (20 mg/mL) (Qiagen Singapore Pte. Ltd, Cat. No. 19133), gently mixed by flicking of tube and incubated at 56 °C for 20 min ...
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bioRxiv - Evolutionary Biology 2019Quote: Total DNA was extracted from tadpole tails by overnight digestion in 10% proteinase K solution at 56 °C and extracted using the Qiagen Biosprint 96 DNA blood kit (Qiagen, CA, USA). DNA was eluted in 100 - 200 μL buffer AE (QIAgen).
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was extracted from the tongue tissue after treating the tissue with proteinase K using a Qiagen Genomic Tip Kit for High Molecular Weight DNA following Qiagen’s extraction protocol (Qiagen, Valencia, CA, USA). After extraction ...
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bioRxiv - Physiology 2020Quote: ... Sections were then post-fixed in 4% PFA/PBS before washing with buffer containing Proteinase K (PK; Qiagen UK Ltd, Manchester, UK) (1 µg/ml PK ...
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bioRxiv - Systems Biology 2021Quote: ... The liquid was aliquoted into a microcentrifuge tube with 590 µl nuclease-free water and 10 µl Proteinase K (QIAGEN, Hilden, Germany). For RNA extraction from swabs ...
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bioRxiv - Zoology 2021Quote: ... dead mites were pierced with an 18-gauge needle under a dissecting microscope and digested overnight in lysis buffer and proteinase K at 56 °C (Qiagen, Valencia, CA). Final DNA product from each mite was eluted in 60 µL of AE buffer ...
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bioRxiv - Microbiology 2020Quote: ... of a 6-well plate in 200 μL ice-cold PBS + 10% Proteinase K and processed through the DNeasy Blood and Tissue Kit following manufacture protocols (QIAGEN, Hilden, Germany). gDNA was stored at −20° C for short-term storage or −80° C for long-term storage.
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bioRxiv - Microbiology 2020Quote: Total RNA extraction was performed using a squash buffer (10 mM Tris base, 1 mM EDTA, 50 mM NaCl) supplemented with 1:8 part Proteinase K (Qiagen, 15mg/ml). Mosquito abdomens with the midguts and heads with thoraces were individually collected in 50 μl of squash buffer at 7 and 14 days ...
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bioRxiv - Microbiology 2022Quote: ... of a 6-well plate in 200 μL ice-cold PBS + 10% Proteinase K and processed through the DNeasy Blood and Tissue Kit following manufacture protocols (QIAGEN, Hilden, Germany). gDNA was stored at -20° C for short-term storage or -80° C for long-term storage.
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Genomic local adaptation of a generalist plant species to pollinator communities and abiotic factorsbioRxiv - Plant Biology 2022Quote: ... 750 ng of purified gDNA was labelled with DLE-1 labelling mix and subsequently incubated with Proteinase K (Qiagen, cat. no. 158920) followed by drop dialysis ...
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bioRxiv - Molecular Biology 2023Quote: ... 600 µl of lysis buffer (TES (0.1 M TRIS, 10 mM EDTA, 2% sodium dodecyl sulphate; pH 8) and Proteinase K (Qiagen, 20 mg/ml) in a 20:1 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was treated with proteinase K at 42°C for 2 hrs and purified using MinElute PCR Purification Kit (Qiagen, Cat# 28004).
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bioRxiv - Microbiology 2023Quote: ... Bacterial cells were lysed by enzymatic lysis and proteinase K treatment and total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) with subsequent DNAse treatment using the RapidOut DNA removal kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: Genomic DNA from MCF7 cells was isolated by proteinase K digestion at 65°C for 30 minutes followed by purification using the QIAamp circulating nucleic acid kit (Qiagen, Venlo, The Netherlands). Genomic DNA from frozen tissue sections of colorectal liver metastases was isolated using the NucleoSpin Tissue kit according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2020Quote: ... about 20 mg of tissue was macerated with sterile blade and digested with 30 μl of Proteinase K (20mg/ml) and 300 μl of ATL buffer (Qiagen Inc., Hilden, Germany) overnight at 56°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was digested overnight at 56°C in 20 ul proteinase K and 180 ul Buffer ATL from the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Valencia, California, USA). DNA was extracted from the digest with the QIAamp PowerFecal DNA Kit (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl, QIAGEN, >600 mAU / ml) and left at 55 °C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Buffer G2 and proteinase K were incubated at 56°C overnight and extracted using the EZ1 DNeasy Blood Tissue Kit (Qiagen GmbH, Hilden, Germany) in line with the manufacturer’s protocol.
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bioRxiv - Animal Behavior and Cognition 2020Quote: Genomic DNA was extracted and purified from mouse tails by a sodium hydroxide method or by proteinase K digestion followed by high-salt precipitation (Gentra/Qiagen, Valencia, CA, USA). In total ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We extracted and purified genomic DNA from tail tissue either using a sodium hydroxide method (Truett, Heeger et al. 2000) or by proteinase K digestion followed by high-salt precipitation (Gentra/Qiagen, Valencia, CA, USA). We measured the concentration and purity of DNA using a small-volume spectrophotometer (Nanodrop ...
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bioRxiv - Evolutionary Biology 2019Quote: ... with 5 µl proteinase K (20 mg/ml) and lysed overnight at 56°C under shaking in Cell Lysis Solution (Qiagen Gentra Puregen Cell kit) with proteinase K (20 mg/ml) ...
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bioRxiv - Zoology 2023Quote: ... Then 25-50 mg of rehydrated tissue was cut into small pieces with a sterile disposable scalpel blade and transferred into individual 2 ml Eppendorf tubes containing 180 µL of ATL and 20 µL of proteinase K provided by the IndiSpin® QIAcube® HT Pathogen Kit (Qiagen, Courtaboeuf, France). Tubes were incubated between 6 and 12 h at 56°C until full proteolysis was achieved ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from homogenates after overnight incubation with 20 μl of proteinase K at 56°C using the QIAamp DNA Micro Kit (Qiagen, Hilden, Germany, cat. No. 56304) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Microbiology 2021Quote: ... whole ticks were bisected using a sterile 16-gauge needle and incubated in a lysis buffer (i.e., 180 μl Qiagen Buffer ATL and 20 μl proteinase K) at 56°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated in a 50 µL reaction with or without triton-x (1% v/v) and with or without proteinase K (Qiagen #19131, final concentration 20 µg/mL) for 15 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... 25 µL of samples was mixed with Triton X-100 solution and then incubated with 25 µL of Proteinase K (ProK; Qiagen, 19131; 100 µg/mL in PBS) at 37°C for 30 min prior to Tluc measurement.