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Citations for Qiagen :
1 - 50 of 61 citations for cis Clopidogrel MP 13C d3 Derivative Pair of Enantiomers since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... pBABE derivatives were transfected with Effectene (Qiagen) according to the manufacturer’s protocol along with VSVG packaging plasmid into 293-GP cells for generation of retrovirus as described(Morgenstern and Land ...
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bioRxiv - Neuroscience 2022Quote: ... and 15μL of 100ng/mL d3-CBD (Qiagen TissueLyser II (30 hz, 9 min)) ...
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bioRxiv - Neuroscience 2023Quote: ... and gene-specific primer pairs obtained from Qiagen. Real-time qPCR reactions were then run on a CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: UPEC CFT073 and its derivatives were treated with RNAprotect Bacterial Reagent (Qiagen, CA), and RNA was extracted using an RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... and their passaged derivatives using the Qiagen RNeasy kit (Qiagen, Valencia, Ca, USA). HCV RNA was amplified by RT-PCR using Accuscript (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... served as hosts for derivatives of plasmids pGEX-6P-1 (Cytiva) and pQE30 (Qiagen), respectively ...
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bioRxiv - Cell Biology 2021Quote: pBABE derivatives containing empty IRES_EGFP or different Cdc20_IRES2_EGFP constructs were transfected with Effectene (Qiagen) along with VSVG packaging plasmid into 293-GP cells for generation of retrovirus as described 29 ...
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bioRxiv - Neuroscience 2021Quote: ... The primer pairs for specific genes were purchased from Qiagen (QuantiTect Primer Assay ...
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bioRxiv - Immunology 2021Quote: ... DARPin clones selected by ribosome display were cloned into a derivative of the pQE30 (Qiagen) expression vector ...
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bioRxiv - Cancer Biology 2024Quote: ... The following primer pairs were from Qiagen (RT2 qPCR primer assay): GPI (PPH00897C) ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged BASP1 derivatives were prepared using Ni-NTA beads following the manufacturers instructions (Qiagen). Purified proteins were dialysed into Buffer D and stored at −80°C.
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bioRxiv - Genomics 2020Quote: ... Pairs were pairwise aligned in CLC Genomics Workbench 12 (Qiagen, Hilden, Germany) using default settings (gap open cost=10 ...
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bioRxiv - Cancer Biology 2024Quote: ... and a ChemiDoc MP Imaging System (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: ... Specific primer pairs (miScript Primer Assay or miRCURY LNA miRNA PCR Assay, Qiagen) for miR-142-5p and RNU48 were used ...
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bioRxiv - Cancer Biology 2022Quote: ... the respective Quantitect Primer pairs for detection of specific mRNAs (Qiagen, Hilden, Germany), and StepOnePlusTM qPCR system (2-ΔΔCT method) ...
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bioRxiv - Microbiology 2023Quote: ... aureus CIs were extracted using DNeasy Blood & Tissue Kit (Cat. #69504, Qiagen Pty. Ltd, Australia) according to the manufacturer’s guidelines with slight modifications ...
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bioRxiv - Cancer Biology 2024Quote: ... The final enriched pool of cDNAs encoding putative DARPin binders was cloned into a bacterial pQE30 derivative vector (Qiagen) via unique BamHI and HindIII sites as a fusion construct with an N-terminal MRGS(H)8-tag and a C-terminal Flag-tag containing a T5 lac promoter and lacIq for expression control ...
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bioRxiv - Genetics 2022Quote: ... and band size around 100 base pairs was excised and gel purified (Qiagen #27206). Purified PCR products were TA-cloned using the pGEM-T Easy Vector System (Promega ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells in 6-well plates were transfected with 2 μg pIRES2-eGFP or Trim-HA-NUP153 derivatives using Effectene (Qiagen). At 24 h post-transfection ...
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bioRxiv - Microbiology 2022Quote: The final enriched pool of cDNA encoding putative DARPin binders was cloned as fusion construct into a bacterial pQE30 derivative vector (Qiagen), containing a T5 lac promoter and lacIq for expression control ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from WT427 cas9 BSFs and three clonal derivatives for each gene using the DNeasy Blood and Tissue extraction kit (Qiagen). 5 µM each of gene-specific forward and reverse primers and 30 ng of gDNA was mixed with 0.4 µl Phire Hot Start II polymerase ...
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bioRxiv - Cell Biology 2022Quote: ... MPs and exosomes with the RNeasy mini kit (Qiagen) and eluted from the column in RNase ...
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bioRxiv - Cancer Biology 2020Quote: ... Expression of PAINT was determined using PAINT-specific primer pairs (RT2 qPCR Primer Assays - Qiagen) and internal control EIF3D and RPL13A specific primers (RT2 qPCR Primer Assays -Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was extracted from 20 pairs of dissected ovaries using the RNeasy Micro Kit (QIAGEN). RNA from w1118ovaries served as control.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded at 3 × 105 cells.well−1 in 6-well plates and transfected 24 h later with one pmiRGLO derivative construct per well (0.9 µg plasmid) using Effectene (Qiagen cat. #301425). After 3 days the cells were washed with PBS and lysed with Cell Culture Lysis Buffer (Promega cat ...
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bioRxiv - Genomics 2022Quote: Genomic DNA for Illumina mate-pair sequencing was extracted using the DNeasy Plant Mini Kit (Qiagen) from 8-week-old leaves of ALO seedlings ...
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bioRxiv - Microbiology 2021Quote: ... The following primer pairs were used and data were analyzed with Rotorgene 6000 Series Software (Qiagen): GAPDH ...
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bioRxiv - Immunology 2020Quote: Primer pairs were used in this study together with a QuantiFast SYBR green PCR kit (QIAGEN). The sequences of primer pairs used in this study are listed in Supplementary Table 3 ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNAs containing HIV-1 proviruses were recovered by infecting viruses produced in derivatives of THP-1 or SupT11 cells into SupT11 using DNeasy Blood & Tissue Kits (Qiagen, Cat# 69504). Following DpnI digestion ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from 0.25 g of soil from all segments from two of the five 13C soil cores using the DNeasy PowerSoil DNA Extraction kit (Qiagen, Hilden, Germany) to determine the segment with the highest abundance of methanogens or methanotrophs based on the respective functional marker genes using qPCR as described below ...
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bioRxiv - Molecular Biology 2021Quote: ... The pair-wise sequence alignments were performed using Alignment and trees tool of CLC genomics workbench (Qiagen). The promoter sequences were analyzed for the presence of putative cis-regulatory elements by PlantCARE (Rombauts et al ...
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bioRxiv - Immunology 2020Quote: 4 matched pairs of Treg and MulTreg were expanded and RNA extracted (RNeasy RNA extraction kit, Qiagen). cDNA was then produced (SMART cDNA synthesis kit ...
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bioRxiv - Genomics 2023Quote: ... the 2 pairs of bacteriomes were dissected and DNA was extracted using the DNEasy Blood & tissue Kit (Qiagen). DNA library was constructed using the NGS Nextera Flex DNA library preparation kit (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... Reads of short length (<900 base pairs (bp)) were manually discarded using CLC Genomics Workbench (v. 12.0.03) (QIAGEN).
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bioRxiv - Genetics 2020Quote: ... Multiplex polymerase chain reaction (PCR) was performed using three primer pairs and the Multiplex PCR Kit (QIAGEN, Hilden, Germany). Each reaction contained 2× QIAGEN multiplex PCR master mix ...
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bioRxiv - Neuroscience 2023Quote: ... DNA between 300 and 700 base pairs was then cut and purified using the Qiagen Gel Extraction Kit (Qiagen). Library sequencing was performed at the RNomics platform of the Université de Sherbrooke (Canada) ...
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bioRxiv - Evolutionary Biology 2020Quote: A subset of 1.29 × 109 read pairs (approximately 45× genome coverage) were assembled with the CLC Genomics Workbench (v. 9.5.3) (Qiagen, Hilden, Germany) using default parameters ...
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bioRxiv - Genomics 2020Quote: Bisulfite converted DNA was amplified with a region-specific biotinylated/unmodified DNA primer pair (Metabion; Supplemental Table S4) using the PyroMark PCR Kit (Qiagen) according to the manufactures instructions ...
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bioRxiv - Biochemistry 2020Quote: ... low melting point agarose gel and the approximately 700 base pair band corresponding to the heavy chain only immunoglobulin was extracted and purified using QIAquick Gel Extraction Kit (Qiagen). A secondary amplification of the VHH domain was performed using modified primers (VHH-Esp-For ...
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bioRxiv - Biophysics 2020Quote: ... Total RNA from each pair of AM from a single animal was isolated using the RNeasy Plus Mini Kit (Qiagen). cDNA was synthesized using the BioRad iScript cDNA Synthesis Kit (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.2 μM gene specific primers pairs (hVDAC1, hVDAC2, hVDAC3, β-actin (Table 1) and 12.5 μl of master mix (QuantiFast SYBR Green PCR kit, Qiagen). Three independent experiments of quantitative real-time were performed in triplicate for each sample ...
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bioRxiv - Microbiology 2020Quote: ... templates containing a T7 phage promoter sequence (5’-TAATACGACTCACTATAGGG-3’) were amplified by PCR using corresponding primer pairs and were purified with a QIAquick PCR purification kit (Qiagen). The primers used are listed in S1 Table ...
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bioRxiv - Immunology 2021Quote: ... The band corresponding to 257 base pairs was cut out and DNA (NGS library) was extracted using a QIAquick Gel Extraction Kit (Qiagen) according to standard manufacturer protocols ...
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bioRxiv - Genetics 2021Quote: ... Specific primers were designed to cover approximately 500 base pairs around the mutations and the regions were PCR-amplified using Qiagen Multiplex PCR kit (Qiagen). The PCR products were sequenced with corresponding primers using canonical Sanger sequencing at SeqLab-Microsynth (Göttingen ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.2 uM gene specific primers pairs (hVDAC1, hVDAC2, hVDAC3, ß-actin) and 12.5 ul of master mix (QuantiFast SYBR Green PCR kit, Qiagen). Three independent experiments of quantitative real-time were performed in triplicate for each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified cDNA was subsequently isolated by separation in a 3% agarose gel and isolation of cDNAs 200 to 600 base pairs in length (corresponding to 100 to 500 nucleotide RNAs plus adaptors) using QIAquick gel extraction kit (Qiagen) according to manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... was digested using the same restriction enzyme pair and all digests were gel extracted using QIAquick Gel Extraction Kit (Qiagen). Gel extracts of vector and trib digests were ligated into pCS2+ via overnight incubation at room temperature with T4 DNA ligase according to manufacturer’s instructions (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... Genomic DNA for sequencing was prepared from 25 L3 salivary gland pairs or 10 mg embryos (0-6 h AED) using DNeasy Blood and Tissue kit (Qiagen). Each sample was prepared in triplicate ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were separated on a 2% agarose gel and fragments from 600-800 base pairs were isolated using the QIAquick Gel Purification Kit (Qiagen). Fragments were then amplified by 15 cycles of PCR and sequenced using Illumina sequencing at the Tufts Core Facility (Boston ...
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bioRxiv - Plant Biology 2021Quote: ... Amplification of a single product of the expected size by a primer pair was first confirmed by RT-PCR using Qiagen® One-Step RT-PCR kit (Qiagen) followed by agarose gel analysis of the amplified products ...