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Citations for Qiagen :
251 - 300 of 990 citations for Zika Virus Envelope Protein Suriname strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Reads were mapped against the reference genome of strain S288C and variants detected using CLC Genomics Workbench (Qiagen).
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bioRxiv - Microbiology 2023Quote: Genomic DNA was isolated from the clinical strains obtained using DNeasy Blood and tissue kit (Qiagen cat. 69504). DNA was sent to Microbial Genome Sequencing Center ...
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bioRxiv - Genomics 2023Quote: ... or the buffy coat of the BALB/c strain using the QIAsymphony DNA Mini Kit (cat:931236, Qiagen). The extracted gDNA was sheared to 150 bp in size using a Covaris LE 220 instrument ...
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bioRxiv - Microbiology 2024Quote: ... and mapping of the reads on a reference (GenBank strain: U39292) using CLC genomics workbench software v.21.0.5 (Qiagen). Parameters for reference-based assembly consisted of match score = 1 ...
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bioRxiv - Microbiology 2021Quote: ... To generate a recombinant A_NS237 virus, viral RNA was extracted from allantoic fluid using Trizol reagent (Thermo Fischer, Germany) and Qiagen RNeasy Kit (Qiagen, Germany) following the manufacturers’ guidelines ...
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bioRxiv - Genomics 2020Quote: ... Viral RNA was extracted from 300 µl of viral transport media (VTM, Himedia, India) using QIAamp 96 Virus QIAcube HT Kit (Qiagen, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: A 200 μL aliquot of Copan UTM® was processed through the QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen, Cat# 937036) following manufacturer’s instructions on the QIAsymphony SP instrument ...
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bioRxiv - Molecular Biology 2020Quote: The RNA isolation was performed with 50 µl of the crude extract on the QIAsymphony with the DSP Virus/Pathogen Kit (Qiagen, #937055). The RT-PCR was performed using 5 µl of 85 µl eluate with TIB MolBiol Lightmix® MODULAR SARS AND WUHAN CoV E-Gene Kit ...
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bioRxiv - Genomics 2020Quote: Viral RNA was extracted from nasopharyngeal swabs of patients with COVID-19 using the EZ1 DSP Virus Kit (Qiagen, Hilden, Germany), optimized for viral and bacterial nucleic acids extractions from human specimens using magnetic bead technology ...
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bioRxiv - Microbiology 2021Quote: ... we collected the cell culture supernatants after 24 h of infection for viral RNA extraction with a QIAamp 96 Virus QIAcube HT Kit (Qiagen, 57731) and for viral RNA copy number detection in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from the lungs of influenza virus-infected mice using the RNeasy Mini Kit (QIAGEN, Cat. No 74004) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... viral RNA was extracted from the egg-isolate of the virus from the nasal swab using the QIAmp viral RNA mini-kit without the addition of carrier RNA (Qiagen, Manchester, UK). cDNA was synthesized from RNA using a random hexamer primer mix and cDNA Synthesis System (Roche ...
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Host transcriptomic profiling of COVID-19 patients with mild, moderate, and severe clinical outcomesbioRxiv - Genomics 2020Quote: RNA was extracted from nasopharyngeal swabs using the QIAamp Viral RNA Mini or the 213 EZ1 DSP Virus Kits (Qiagen, Hilden, Germany). All patients in this study tested positive for SARS-CoV-2 by RT-qPCR performed at Dubai Health Authority Hospitals ...
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bioRxiv - Molecular Biology 2020Quote: ... The entire 200-µL reaction was used as input for ssAAV gDNA extraction using a MinElute Virus Spin Kit (Qiagen Cat#57704). 50-ng of ssDNA from each sample were bisulfite converted using the EZ Methylation Gold kit (Zymo Cat#D5005 ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was extracted from virus library and ferret nasal wash samples using the QIAamp® Viral RNA mini kit (Qiagen, Germany). Next generation sequencing was carried out twice on the nasal washes ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA was purified using the QIAamp 96 virus QIAcube HT Kit and processed on the QIAcube robotic extraction platform (QIAgen, Hilden, Germany)
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bioRxiv - Microbiology 2022Quote: ... A wild-type virus stock NL4-3 was prepared by transfection of the pNL4-3 plasmid (purified using the Qiagen Plasmid Maxikit) into HeLa cells ...
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bioRxiv - Immunology 2019Quote: ... RNA was isolated from plasma samples using the QIAsymphony Virus/Bacteria Midi kit on the QIAsymphony SP automated sample preparation platform (Qiagen, Hilden, Germany). RNA was extracted manually if plasma volumes were limited ...
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bioRxiv - Genomics 2020Quote: All 49 COVID-19 patients tested positive for SARS-CoV-2 by RT-qPCR using RNA extracted from nasopharyngeal swabs following the QIAamp Viral RNA Mini or the EZ1 DSP Virus Kits (Qiagen, Hilden, Germany). RNA libraries from all samples were then prepared for shotgun transcriptomic sequencing using the TruSeq Stranded Total RNA Library kit from Illumina (San Diego ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from 200 ul of oral swab material using the Qiagen EZ1 DSP Virus kit on the automated EZ1 XL Advance instrument (Qiagen, Valencia, CA). Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA and DNA were extracted from fecal and urine samples using the QIAamp MinElute Virus Spin Kit (Qiagen, Valencia, CA, USA), but using 20μg of linear polyacrylamide (VWR ...
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bioRxiv - Microbiology 2022Quote: Viral RNA was extracted from 200 ul of nasopharyngeal swab and BAL material using the Qiagen EZ1 DSP Virus kit on the automated EZ1 XL Advance instrument (Qiagen, Valencia, CA). Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from stock virus in allantoic fluid using the QIAamp Viral RNA Mini Kit (Qiagen Inc., Valencia, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... whereupon DNA was subsequently purified from 200 μl of the lysate using the QIAsymphony DSP Virus/Pathogen extraction kit (Qiagen Cat# 937036) on the QIAsymphony automated platform ...
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bioRxiv - Molecular Biology 2023Quote: Total viral genomic DNA was extracted from 100 μL stored plasma samples with the QIAamp MinElute Virus Spin Kit (Qiagen, Hilden, Germany), according to manufacturers’ instructions ...
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bioRxiv - Immunology 2024Quote: Virus RNA from in vitro or in vivo experiments was isolated using the QIAamp® Vira RNA Mini Kit (Qiagen, Germantown, MD) or the MagMAX-96 AI/ND viral RNA isolation kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was extracted from Leptospira interrogans serovar Copenhageni strain M20 culture by using DNeasy Blood & Tissue kit (Qiagen) and used as a template to amplify lipl21 ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from P15 liver tissue (n=4 biological replicates per strain) using QIAzol Lysis Reagent (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated from selected putative mutants and the parent strains using the DNeasy Blood & Tissue kit (Qiagen). Library preparation and 2×150 bp paired-end Illumina sequencing was performed by the Microbial Genome Sequencing Center (Pittsburgh ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from strains marked with KI in Table S1 was extracted using DNeasy Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s guidelines for Gram-negative bacteria ...
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bioRxiv - Microbiology 2021Quote: DNA was isolated from the PA14 ΔlasI strain lysogenized by phage JBD4439* using the DNeasy Blood & Tissue kit (Qiagen) followed by treatment with 100 μg/mL RNAse A (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: Whole-genome DNA of Muribaculaceae strain MF13079 was extracted with QIAamp BiOstic Bacteremia DNA Kit (12240-50, Qiagen, USA) and sequenced with the Oxford@ Nanopore PromethION platform (performed by Nextomics Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was isolated in six biological replicates per strain from 0.5 mL of culture using the RNeasy Protect Bacteria Mini kit (Qiagen) on six consecutive days ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated from four replicate cultures for each strain grown to mid-exponential phase in THY (OD ∼ 0.5) using the RNeasy kit (Qiagen) and processed as previously described (Horstmann et al. ...
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bioRxiv - Genomics 2022Quote: Long fragment DNA extraction from sub-cultured strains was performed using the Qiagen Genomic tip 100/G kit (Qiagen). Quality and fragment length assessments were measured with the Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from bacterial strains using a Qiagen Genomic DNA Buffer Set (Qiagen, Germany; Cat. No. 19060) and Qiagen genomic tip 100/G gravity-flow ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial biomass from strains grown on SKV agar plates was resuspended directly in 300 μl of PowerBead Solution (Qiagen) and vortexed to mix ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from the two isolated strains using a DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) and sequenced using an Illumina HiSeq sequencer (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... total RNA from WT or Pcu-shc::Δshc strains at early exponential phase (OD600 = 0.5-0.8) was extracted using a RNeasy® Mini Kit (Qiagen) according to the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2023Quote: Bacterial genomic DNA from each strain was purified individually using the QIAamp Fast DNA Stool Mini Kit (Qiagen, #51604). DNA was quantified by Nanodrop ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we extracted DNA for other strains and purified it using a Blood and Cell Culture DNA mini kit (Qiagen). Finally ...
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bioRxiv - Microbiology 2023Quote: ... 37 °C) of strain GFKo1 grown in nutrient broth (Oxoid) using the Qiagen DNeasy Blood and Tissue Kit (Qiagen). Extracted DNA was adjusted to a concentration of 0.2 ng/μL and treated using the Nextera XT DNA library preparation kit (Illumina ...
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Altered motility in response to iron-limitation is regulated by lpdA in uropathogenic E. coli CFT073bioRxiv - Microbiology 2023Quote: ... Strains were cultured for five hours at 37°C with aeration before harvest and treatment with bacterial RNAprotect (Qiagen). Bacterial pellets were stored at -80°C until RNA isolation.
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bioRxiv - Microbiology 2023Quote: ... coli BL21 Star (DE3) strain and purified by Ni-NTA affinity chromatography with a Ni-NTA agarose column (QIAGEN), followed by size exclusion chromatography with a HiLoad 26/600 Superdex 75 pg column (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting plasmid was then transformed into E.coli DH10b strain for isolation via QIAprep Plasmid Mini-Prep Kit (Qiagen). The isolated plasmid was subsequently transformed into D.radiodurans R1 chemically competent cells (above) ...
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bioRxiv - Microbiology 2024Quote: ... was used to amplify DNA fragment except for PCR verification of plasmids or strains for which Taq polymerase (Qiagen) was used ...
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bioRxiv - Bioengineering 2021Quote: ... Protein extraction was conducted using a Qproteome Bacterial Protein Prep Kit (Qiagen)‡ ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein isolation with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: ... Protein-protein interaction networks were built using Ingenuity Pathway Analysis (IPA) (Qiagen).
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bioRxiv - Genetics 2021Quote: ... Protein Precipitation Solution (Qiagen) was added at 0.33x and mixed well ...