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Citations for Qiagen :
301 - 350 of 991 citations for Zika Virus DIII Envelope Protein Asian strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Protein Precipitation Solution (Qiagen) was added at 0.33x and mixed well ...
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bioRxiv - Microbiology 2020Quote: ... Viral nucleic acids were then isolated using the Qiagen QIAamp MinElute Virus Spin Kit without the use of AW1 buffer or carrier RNA (Qiagen, Valencia, CA, USA). Random hexamers were used to prime cDNA synthesis (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in 384 well plates on an Applied Biosystems 7900HT Fast Real-Time PCR system using the QuantiTect Virus Kit (Qiagen, Redwood City, CA) and SARS-CoV-CDC RUO primers and probes (Integrated DNA Technologies (IDT) ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acids were extracted at CNM using either QIAamp MinElute Virus Spin (DNA) or QIAamp Viral RNA Mini kits (Qiagen, Germantown, MD, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted for the ancestral strain (AN) and a single clonal isolate from each evolved population (S1-S10) using QIAamp DNA Blood Midi Kit (Qiagen) following the manufacturer’s recommendations from 50 mL culture incubated at 17 °C for 5 days in 75 cm2 flasks ...
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bioRxiv - Genomics 2022Quote: ... The genomic DNA of the wild-type and the mutant strains was isolated using a QIAamp DNA Mini Kit (QIAGEN). Libraries were prepared using the Nextera XT DNA Library preparation kit (Illumina) ...
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bioRxiv - Molecular Biology 2020Quote: ... coli strain (Rosetta) and purified using Ni-NTA affinity chromatography according to the manufacturer’s instructions (Qiagen Cat No./ID: 30210). Purified protein was used as an immunogen to raise polyclonal antibody in rabbits at a local commercial facility (DPL ...
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bioRxiv - Microbiology 2019Quote: ... The complete 16S rRNA gene was amplified in specific strains after DNA extraction using the DNeasy Blood & Tissue kit (Qiagen), following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2019Quote: Genomic DNA from each strain was isolated from overnight cultures using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was prepared from 20 adult female flies from each strain using the DNeasy Blood and Tissue kit (Qiagen). PCR was performed with primers specific to the LcαE7 gene16 ...
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bioRxiv - Microbiology 2021Quote: ... 500 μL of mid-log phase (OD600nm = 0.45 - 0.7, strain-specific) cells were stabilized using RNA Protect Bacteria Reagent (QIAGEN, Hilden, Germany), and cell pellets stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... and RNA was isolated from 3 biological replicates of each strain when the cultures had reached OD600 = 0.1 using the RNeasy mini kit (Qiagen #74106). RNA-Seq was performed at the Genomics Core Facility at Princeton University ...
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bioRxiv - Cell Biology 2020Quote: RNA was isolated from frozen RNA later® soaked tissue from the embryo from either C57BL/6NCrl or Ddi2PD strains using RNeasy plus Micro and Mini Kits (QIAGEN). Samples with RIN values above 7 in the Bioanalyzer RNA 6000 Nano assay (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant protein fragments were produced in Escherichia coli (strain M15) as fusion products with an N-terminal tag of six histidines using plasmid pQE30 (Qiagen) and purified from total bacterial lysates by nickel affinity chromatography ...
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bioRxiv - Microbiology 2022Quote: ... Transformation of gene knockouts from strains 168 or PY79 into strain 3610 was done with the following protocol: DNA was extracted from strain 168 or PY79 with the DNeasy Blood & Tissue kit (Qiagen) as described in the manufacturer’s protocol and the final DNA yield was quantified by Qubit (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... all clinical isolates and the UCBPP-PA14 reference strain were cultivated at 37 °C in LB broth and harvested in RNAprotect (Qiagen) at OD600= 2 ...
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bioRxiv - Epidemiology 2019Quote: The genomic DNA of the 11 outbreak strains was extracted using the DNeasy blood and tissue kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from cultured tprAko-SS14 or wild-type strains propagated in 6-well plates following the transformation procedure was performed using the QIAamp mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Most DNA samples for ONT sequencing were extracted from 100 female flies from each strain using the Blood and Cell Culture DNA Mini Kit (Qiagen) following manufacturer’s instructions with small modifications (Table S2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we designed strain-specific primers (see Supplementary Table 2) and generated overlapping amplicons using the OneStep RT-PCR Kit (Qiagen) and 5 µL of cDNA ...
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bioRxiv - Biochemistry 2021Quote: Genomic DNA was isolated from 2 clones of each strain corresponding to 50 OD600 units with the Genomic DNA Buffer Set and Genomic-tip 100/G (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The gDNA of all mutants strains constructed in this study was extracted using DNeasy® Blood and Tissue kit (Qiagen) according to the manufacturer recommendations ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA from 110 mutants plus the ancestral strain was extracted with the Genomic-tip 20/G Kit (Cat. No. 10223, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... coli HST04 dam-/dcm- strains in which target MTases were transformed were extracted using the DNeasy UltraClean Microbial Kit (QIAGEN) according to the supplier’s protocol after induction of gene expression ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from MU636 and set1 mutant strains growing in plates using the RNeasy Plant Mini Kit (Qiagen) and treated with DNase (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... We extracted gDNA from the MSK named strains collected in Botswana and North Carolina using the Powersoil Pro extraction kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA from mutant clones with enhanced tdTomato-ATG8 signal compared to the parental strain was harvested using DNeasy Blood & Tissue Kit (Qiagen) and send for sequencing at UMICH Advanced Genomics Core ...
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bioRxiv - Molecular Biology 2024Quote: ... coli XL1-blue strain was transformed and plasmids were isolated from bacterial cultures using the Plasmid Midi Kit (Qiagen, Germany). Sequencing of the three NS1 plasmids was performed by Eurofins (Germany).
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bioRxiv - Cancer Biology 2022Quote: Whole tissue protein was extracted by Qproteome Mammalian Protein Prep Kit (Qiagen, 37901) according to the manufacture’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein were extracted using the AllPrep DNA/RNA/Protein kit (Qiagen, #47054). Sample concentrations were measured with Qubit high sensitivity dsDNA and RNA platform ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated by adding 100 μL of a protein precipitation solution (Qiagen). Samples were centrifuged for 5 min at 13000 rpm ...
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bioRxiv - Genomics 2021Quote: ... and Vero E6 cells infected with 2 patient virus isolates was converted to cDNA using reverse transcriptase from qiaseq SARS-CoV-2 primer pool (Qiagen kit, cat no. 333896). APOBEC3b (sense ...
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bioRxiv - Genomics 2020Quote: ... was extracted from the liver of a female Pekin duck (Anas platyrhynchos, Z2 strain) with Gentra Puregene Tissue Kit (Qiagen #158667). Libraries for SMRT sequencing were constructed as described previously[87] ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain BL21(DE3)(Novogen Inc.) essentially as described [34] except that after elution from Ni-NTA agarose resins (Qiagen Inc.) by incubating with 400 mM imidazole for 1 hr at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: The gDNA of strains obtained during the IVEE at both temperatures was extracted with DNeasy® Blood and Tissue Kit (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2019Quote: DNA was extracted from 94 RIAILs and the two parental strains with the DNeasy® Blood & Tissue Kit (QIAGEN, Hilden, Germany). For each strain ...
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Genetic architecture of oxidative stress tolerance in the fungal wheat pathogen Zymoseptoria triticibioRxiv - Genetics 2020Quote: ... Sequence variation among the parental strains in the QTL confidence intervals was identified by performing sequence alignments (CLC Sequence Viewer 7.6.1, QIAGEN Aarhus A/S). The previously published in planta expression data (Palma-Guerrero et al ...
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bioRxiv - Microbiology 2022Quote: Complete genome of strain YC6860T was sequenced by extracting its high-quality genomic DNA with the blood and cell culture DNA minikit (Qiagen, MD). The genome of YC6860T was sequenced and characterized using Illumina GAIIx sequencer (San Diego ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from fibroblasts obtained from each individual mouse from each of the five non-CC inbred strains using AllPrep DNA/RNA Mini kit (Qiagen, #80204). The concentration and purity of extracted DNA was determined using a NanoDrop 2000 UV-vis spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: The genomic DNA of the CRISPR-Cas3 engineered strains after vector curing was isolated using the DNeasy UltraClean Microbial Kit (Qiagen, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA from the pooled recombinants and parent strain were isolated using the Gentra® Puregene® DNA isolation kit (Qiagen) and sent for whole genome sequencing (BGI) ...
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bioRxiv - Genomics 2020Quote: ... Protein was precipitated by adding 200 µL of ice-cold Protein Precipitation Solution (Qiagen), gentle mixing and incubation on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The peptide-coupled proteins were separated from uncoupled proteins using Ni-NTA Agarose (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... N-terminal His-tagged proteins were purified using a QIAexpress protein purification system (Qiagen), as previously described47.
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bioRxiv - Neuroscience 2020Quote: ... Total protein was extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen #80004) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein was purified by the AllPrep DNA/RNA/Protein Mini Kit (Qiagen: # 80004). Corresponding protein expression levels in the cells of different groups were detected by western blot using the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested for total protein using a Qproteome Mammalian Protein Prep Kit (Qiagen). Total protein was quantified using a PierceTM BCA Protein Assay Kit (Cat ...
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bioRxiv - Developmental Biology 2022Quote: ... 333µl Protein Precipitation Solution (Qiagen) was added and samples were vortexed vigorously for 20 seconds at high speed ...
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bioRxiv - Cell Biology 2023Quote: ... Protein Precipitation Solution (Qiagen #158910), DNA Hydration Solution (Qiagen #158914 ...