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Citations for Qiagen :
1 - 50 of 10000+ citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... under p32 promoter to the recombinant expression using PCR Cloning Kit (Qiagen, Germany). The recombinant plasmids were named as pMG36e:nisA (nisA) ...
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bioRxiv - Microbiology 2022Quote: ... Gentra Puregene Yeast/Bact Kit (Qiagen) was used ...
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bioRxiv - Microbiology 2019Quote: ... meningitidis using the Gentra Puregene Yeast/Bact Kit (Qiagen) and plasmid DNA was extracted from E ...
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bioRxiv - Microbiology 2021Quote: ... interrogans using the Gentra Puregene Yeast/Bacteria kit (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... These were cloned into Qiagen’s pDrive cloning vector using the Qiagen PCR Cloning Kit (Qiagen) and clones verified by sequencing (Department of Biochemistry Sequencing Facility ...
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bioRxiv - Cell Biology 2022Quote: Total yeast RNA was extracted using RNeasy Kit (74004, QIAGEN) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the QIAseq FastSelect-rRNA Yeast Kit (Qiagen Cat. 334217). Lastly ...
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bioRxiv - Molecular Biology 2021Quote: ... 1μl of oligonucleotides from the QIAseq FastSelect –rRNA Yeast Kit (Qiagen) in a total of 20.5 μl were incubated in a thermocycler with a preheated lid at 94°C ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted using the PureGene Yeast/Bacteria Kit (Qiagen, Germany), following the manufacturer’s instructions for Gram-negative bacteria ...
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bioRxiv - Microbiology 2019Quote: ... DNA isolation followed instructions of the Gentra Puregene Yeast/Bact kit (Qiagen) with slight modifications ...
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bioRxiv - Genomics 2023Quote: ... rRNA depletion was performed using QIAseq FastSelect -rRNA yeast kit (Qiagen #334215) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The rRNA depletion strategy using QIAseq FastSelect –rRNA Yeast Kit (Qiagen, #334215) was integrated into the RNA fragmentation step of the TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... gDNA was extracted using an isopropanol-ethanol purification kit (Puregene Yeast/Bact. Kit B from Qiagen) and following the manufacturer’s protocol including a 60-minute RNase A treatment ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was isolated from yeast pellet using the RNeasy mini kit (Qiagen) following the manufacturer’s instructions for yeast ...
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bioRxiv - Plant Biology 2022Quote: ... plasmids were rescued from yeast as described using the QIAprep Miniprep Kit (QIAGEN, Chadstone Centre ...
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bioRxiv - Synthetic Biology 2022Quote: ... Yeast genomic DNA for genome sequencing was extracted using Genomic-tip kits (Qiagen). Plasmid DNA was isolated from bacterial hosts using QIAprep Spin Miniprep kits (Qiagen).
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bioRxiv - Microbiology 2022Quote: ... aureus genomic DNA was purified using the Puregene yeast/bacteria kit B (Qiagen). Lysostaphin ...
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bioRxiv - Genetics 2023Quote: ... the Gentra Puregene Yeast Bacteria DNA extraction kit was used (Qiagen, Hilden, Germany). Cells were grown for 16 h in 5 ml cultures and prepared according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... and cloned in pDrive using the Qiagen PCR cloning kit (Qiagen). For the determination of the transcriptional start site 10 individual clones were sequenced.
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bioRxiv - Immunology 2019Quote: ... the inserted coding regions of each gene were transferred to the multi-cloning site of expression vector pQE30 (Qiagen, Hilden, Germany) for the expression of a hexahistidine-tagged protein in E ...
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bioRxiv - Microbiology 2020Quote: DNA extraction was performed using the PureGene® Yeast/Bact kit B (Qiagen, Germany) following the manufacturer’s instructions for extraction of DNA from Gram-negative bacteria and eluted in molecular grade water.
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bioRxiv - Microbiology 2022Quote: Genomic DNA was purified from meningococci with the Gentra Puregene Yeast/Bacteria kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: DNA was extracted using the Gentra Puregene Yeast and Bacteria kit (Qiagen, Hilden, Germany). Illumina libraries with 300-400 bp inserts were generated using the Illumina Nextera XT kit according to the manufacturer’s protocol (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA of the strains was isolated using the Gentra Puregene Yeast Kit (Qiagen). Libraries were made using the Nextera XT DNA Library Prep Kit (Illumina).
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bioRxiv - Cell Biology 2021Quote: ... using the yeast protocol described in the manufacturer’s instructions of RNeasy Mini Kit (Qiagen). To eliminate genomic DNA contamination ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from harvested mycelium using the Gentra Puregene Yeast/Bact kit (Qiagen).
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bioRxiv - Synthetic Biology 2023Quote: ... purified using the Gentra Puregene Yeast/Bacteria gDNA extraction kit (Qiagen catalogue no. 158567). TCSs were amplified by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid DNA was extracted from yeast using Qiagen QIAprep Spin Miniprep Kits (Qiagen, 27104). 500uL Resuspension Buffer PI was added to thawed pellets ...
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bioRxiv - Microbiology 2021Quote: ... Cloning intermediates were purified using the QIAquick PCR Purification kit (Qiagen, #28104).
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bioRxiv - Genetics 2022Quote: ... Ribosomal rRNA was depleted using the QI-Aseq FastSelect –rRNA Yeast Kit (Qiagen cat. 334217). Libraries were sequenced as paired-end 75 base pair reads on a Illumina NextSeq 500 ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA from the isolates was extracted using the Gentra Puregene Yeast/Bact Kit (Qiagen) and used as template for PCRs ...
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bioRxiv - Genetics 2023Quote: ... The PCR for cloning was with UltraRun® LongRange PCR Kit (QIAGEN, 206442) with the following conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA cloning and purification was performed using QIAGEN Plasmid Midi Kit (Qiagen) according to manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from the pellets using GeneiPure Yeast DNA preparation kit (GeNei) or DNeasy blood and tissue kit (Qiagen). The DNA was eluted in the elution buffer (provided with the kit ...
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bioRxiv - Microbiology 2022Quote: ... and genomic DNA was purified using a Yeast/Bact Genomic DNA purification kit (QIAGEN, Hilden, Germany). 1 μg of genomic DNA was digested with the restriction enzyme AatII overnight at 37°C to generate fragments of genomic DNA which were on average 1,500 bp (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from 10 OD600nm units of yeast cells by RNeasy kits (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... each assembled construct was isolated from the cloning strain (QIAprep Spin Miniprep Kit, QIAGEN) and transformed into E ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA isolation and purification was performed using the Gentra Puregene Yeast/Bacteria Kit (Qiagen; Valencia, CA) with slight modification (36) ...
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bioRxiv - Molecular Biology 2020Quote: The small RNAs from the yeast cells (1 x 107) were isolated with the miRNeasy kit (Qiagen) with modifications ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA extraction from isolates was performed using the Gentra Puregene Yeast/Bacteria Kit (Qiagen; Valencia, CA) with slight modification (43) ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression of mRNA was quantified using QuantiTect SYBR Green PCR kit (Qiagen) according to product manual at an annealing temperature of 60 °C ...
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bioRxiv - Cell Biology 2023Quote: ... microRNA expression was quantified using the miScript SYBR Green PCR Kit (Qiagen) using 3 ng of complementary DNA ...
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bioRxiv - Biochemistry 2021Quote: ... The amplified products were cloned into pDrive using the TA Cloning Kit (Qiagen®, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: For the above-described cloning efforts the Qiaquick gel extraction kit (Catalog no. 28706, Qiagen) was used to purify PCR and digestion products from agarose gels ...
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bioRxiv - Cell Biology 2024Quote: ... All DNA fragments used for cloning were purified using a gel purification kit/protocol (Qiagen). All PCR was performed using KOD polymerase (EMD Millipore) ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from 10 ml cultures of yeast strains and purified with an RNeasy kit (Qiagen). The RNA was prepared for sequencing using the TruSeq Stranded Total RNA Sample Prep adaptor kit (Illumina) ...
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bioRxiv - Plant Biology 2020Quote: Bacterial genomic DNA was extracted from overnight (O/N) cultures using the Gentra Puregene Yeast/Bact Kit (Qiagen) (25/74 strains) ...
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bioRxiv - Molecular Biology 2021Quote: ... Further processing was performed with agreement of the RNA extraction protocol using the Qiagen Purification of Total RNA Kit for Yeast with Optimal On-Column DNase Digestion (RNeasy Mini Kit (50) (Qiagen, catalog no. 74104).
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bioRxiv - Plant Biology 2020Quote: ... For expression analysis total RNA was extracted with RNeasy Plant Mini Kit (QIAGEN) from 2-3 weeks old seedlings and 500ng was treated with DNaseI (Ambion ...
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bioRxiv - Plant Biology 2020Quote: ... Gene expression was quantified using the QuantiNova SYBR Green PCR Kit from Qiagen. PCRs were carried out in a Bio-Rad CFX96 machine (C1000 Touch) ...