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Citations for Qiagen :
301 - 350 of 812 citations for West Nile virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Cells lysates were treated with 100 μg/mL of RNase A (Qiagen). Lysates were cleared by centrifugation and then incubated with Glutathione-Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... Cleared lysates were then passed through a Ni-NTA column (Qiagen 30250) that was equilibrated with P5 Buffer pH 7 (20 mM NaHPO4 ...
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bioRxiv - Cell Biology 2019Quote: ... the lysates were collected and incubate with Ni-NTA agarose beads (QIAGEN) for 4 hr ...
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bioRxiv - Cell Biology 2019Quote: ... The cleared lysate was batch bound to Ni-NTA agarose beads (Qiagen) for 1 h at 4oC ...
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bioRxiv - Developmental Biology 2021Quote: ... before the lysate was recovered and passed through a QIA shredder (Qiagen). An equal volume of 100 % ethanol was added to the homogenised lysate before being transferred to a RNeasy extraction column ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were loaded onto a 5 mL Ni-NTA Superflow Cartridges (Qiagen) equilibrated with Buffer B (25 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was incubated with Ni-nitrilotriacetic acid (NTA) resin from Qiagen for 1hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was then subjected to Ni-NTA affinity chromatography (Qiagen) and washed with wash buffer (Na2HPO4 pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was then loaded onto Ni-NTA spin columns (QIAgen) and proteins purified according to manufacturer protocol washing the column with Washing Buffer (30 mM Hepes pH 7.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... QIAshredder homogenizer columns were used to disrupt the cell lysates (Qiagen, 79654). mRNA was purified using the NEBNext poly(A ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA was extracted from cell lysates using RNAeasy purification kit (Qiagen). The quality of RNA was assessed on the Agilent 2100 Bioanalyzer and the RNA was quantified by QuBit fluorometry ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was loaded on a Ni-NTA affinity column (Qiagen). The bound proteins were first washed with lysis buffer and then with the lysis buffer supplemented with 20 mM imidazole to remove non- specific proteins ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was loaded on a Ni-NTA affinity column (Qiagen) and the column was then washed with the lysis buffer and 50 mM imidazole buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and the cleared lysate was incubated with Ni-NTA agarose beads (Qiagen). The protein complex was eluted with buffer containing 30 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... and the cleared lysate was incubated with Ni-NTA agarose beads (Qiagen). After several washing steps in wash buffer (50 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... Lysates were processed using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen). To generate sample libraries ...
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bioRxiv - Microbiology 2022Quote: ... RNA extraction on the lysate was performed with RNeasy Mini Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed with lysis buffer supplemented with 25 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed in two steps with lysis buffer supplemented with 25 mM and 100 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was incubated with 800 μL of Ni-NTA agarose (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified lysate was applied to a Ni-NTA agarose column (Qiagen). The H2A/H2B dimer was eluted from the column with high-salt buffer containing 300 mM imidazole and was incubated with SENP7 protease to remove the His-SUMO-tag.
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bioRxiv - Genetics 2022Quote: ... The lysate was incubated with Ni2+-NTA beads (Ni-NTA Superflow, Qiagen) for 2 h at room temperature with rocking ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified lysate was incubated with 1 ml Ni-NTA resin (Qiagen) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was clarified by centrifugation before adding Ni-NTA-agarose (Qiagen) and incubation for 2 h at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysates after centrifugation were added to equilibrated Ni-NTA Agarose (Qiagen) and mixed gently on a rotary shaker at 4°C for 60 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumor and cell lysates were next transferred onto a QiaShredder column (Qiagen). RNA isolation was performed using the RNA micro plus kit (Qiagen) ...
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bioRxiv - Cell Biology 2019Quote: ... by sonication and clarified lysate was applied to Ni-NTA resin (Qiagen) followed by amylose resin (NEB) ...
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bioRxiv - Genetics 2020Quote: ... total RNAs were extracted from lysates using a miRNeasy kit (QIAgen, #217004), depleted of genomic DNA contamination using Baseline-Zero DNase (Epicentre ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed with lysis buffer supplemented with 25 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed in two steps with lysis buffer supplemented with 25 and 100 mM imidazole ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were purified from lysates on Ni-NTA columns (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... cruzi amastigote/trypomastigote lysate coupled to Carboxy-Ni-NTA beads (Qiagen Inc). Antibody binding was detected with goat antihuman IgG conjugated to phycoerythrin and quantified on a Bio-Plex Suspension Array System (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysate was loaded on a 5 mL Ni-NTA column (Qiagen) equilibrated with buffer C ...
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bioRxiv - Immunology 2021Quote: ... RNA was then extracted from whole lysates using RNEasy kits (Qiagen, Inc.) and combined with mRNA capture beads ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was applied to a Ni-NTA Superflow column (Qiagen). The column was washed with buffer A and a high salt buffer containing 50 mM Tris pH 7.2 ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was incubated with 1 mL of Ni- NTA agarose (Qiagen) for 1 h at 25 °C with rotating ...
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bioRxiv - Molecular Biology 2022Quote: ... The clarified lysate was then loaded onto Ni- NTA spin columns (QIAgen) and proteins purified according to manufacturer protocol washing the column with Washing Buffer (30 mM Hepes pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... and the lysate was spun through a QIAshredder column (Qiagen, cat#: 79656) to fully homogenize ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate supernatants were batch bound with equilibrated Ni-NTA resin (QIAGEN). Washes were performed with 5-10 column volumes of lysis buffer ...
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bioRxiv - Systems Biology 2023Quote: ... with the cell-lysate first being homogenized through a QIAshredder column (Qiagen), per the manufacturer’s directions ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was loaded onto three tandemly linked Strep-Tactin columns (Qiagen) and the complexes were eluted with 2.5 mM desthiobiotin (Sigma ...
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bioRxiv - Immunology 2023Quote: RNA from lysates was purified using RNeasy Plus RNA purification kit (Qiagen) and retrotranscribed using qScript cDNA supermix (Quanta bio) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysate supernatants were applied to packed Ni-NTA beads (QIAGEN; 30210). After washing with 20 column volume of lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... total RNAs were extracted from lysates using a miRNeasy kit (QIAgen, #217004) following the manufacturer’s protocol for recovery of total RNAs (standard protocol) ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was then loaded onto a QIAshredder spin column (#79656, Qiagen) and placed in a 2 mL collection tube before being centrifuged for 2 minutes at full speed ...
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bioRxiv - Systems Biology 2023Quote: ... with the cell-lysate first being homogenized through a QIAshredder column (Qiagen), per the manufacturer’s directions ...
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bioRxiv - Bioengineering 2023Quote: ... RNA was isolated from the lysate with the RNeasy Mini Kit (Qiagen) according to the manufacturers protocol and quantified with NanoDrop 2000 (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from LNSC lysates using the RNeasy Mini Kit (QIAGEN). The TaqMan® RNA-to-CT™ 1-Step Kit (Applied Biosystems ...