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Citations for Qiagen :
51 - 100 of 1134 citations for Water Nuclease Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... The extracted RNA samples were dissolved into 30 µl of nuclease-free water (Qiagen, cat. 129115). RNA concentration and the absorbance ratio at 260/280 nm and 260/230 nm of each sample was measured by NanoDrop™One (Thermo ...
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bioRxiv - Physiology 2023Quote: ... The cDNA used in the qPCR assays was first diluted with nuclease-free water (Qiagen, Hilden, Germany). Each qPCR mixture contained SsoAdvance Universal SYBR® Green Supermix ...
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bioRxiv - Cancer Biology 2020Quote: ... 1μl of forward/reverse primers (5pmol) and 3μl of nuclease-free water were mixed in a 0.1mL real-time PCR (Qiagen) to conduct PCR in a 10μl of reaction volume ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in nuclease-free water and purified using a Qiagen RNeasy kit with additional on-column DNase (Qiagen) digestion ...
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bioRxiv - Genomics 2020Quote: ... cDNA were purified with 1.8X volume AMPure XP Beads and eluted into 42 μl nuclease free water (129114, Qiagen). Next ...
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bioRxiv - Genomics 2020Quote: ... samples were purified with 1.8X volume AMPure XP Beads and eluted into 33 μl nuclease free water (129114, Qiagen). Illumina unique dual barcodes adapters (Cat# 20022370 ...
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bioRxiv - Bioengineering 2022Quote: Nuclei were isolated from differentiating white and brown preadipocytes using an NP-40 based lysis buffer: To 14.7 mL nuclease-free water (Qiagen), 150 uL of Tris-Hydrochloride (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The DNA pellet was washed with 70% (v/v) ethanol and resuspended in 50 μL nuclease free water (Qiagen). DNA concentration and purity were determined by agarose gel electrophoresis and fluorometrically using RiboGreen (Qubit Assay Kit ...
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bioRxiv - Bioengineering 2021Quote: Nuclei were isolated from white and brown preadipocytes using an NP-40 based lysis buffer: To 14.7 mL nuclease-free water (Qiagen), 150 ul of Tris-Hydrochloride (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... 50 μl each input and purified RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen) using the modified protocol as described (Duffy and Simon ...
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bioRxiv - Molecular Biology 2022Quote: ... Biotin-labelled RNA was eluted with 100 μl elution buffer (100 mM DTT in nuclease-free water) and cleaned up with the RNeasy MinElute Cleanup kit (QIAGEN), adjusting the amount of ethanol to capture RNA < 200 nucleotides in length ...
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bioRxiv - Molecular Biology 2019Quote: ... each of forward and reverse primers and 2 μl of nuclease-free water) was run in a Rotor-Gene Q thermal cycler (Qiagen) using 40 cycles of 95°C for 10 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μl each input and purified RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen) using the modified protocol as described (Duffy and Simon ...
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bioRxiv - Molecular Biology 2020Quote: Remaining cell cultures were spun down at 4000 rpm for 5 min and the cell pellet resuspended in 50 μL nuclease-free water (Qiagen). Total RNA was extracted using a hot acidic phenol (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... 50 μl each input and purified RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen) using the modified protocol as described (Duffy and Simon ...
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bioRxiv - Neuroscience 2019Quote: ... were diluted in nuclease free elution buffer (Qiagen) to a 10 ng/µl concentration (200 ng gDNA in 20 µl final volume) ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.5 µl of diluted template cDNA (1:3, nuclease-free water) per real-time PCR reaction (10 µl – SYBR Green PCR kit, Qiagen, 204143) was used to assay specific transcript abundance (CFX96 Real-Time System ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μl of each input and biotinylated RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen #74104) using the modified protocol ...
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bioRxiv - Microbiology 2023Quote: ... RNA pellets were dissolved in 88 μL of nuclease-free water and subjected to genomic DNA digestion with DNase (Qiagen Inc.). RNA samples were then concentrated using RNA Clean & Concentrator (Zymo Research) ...
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bioRxiv - Microbiology 2021Quote: ... in RNAse free water (Qiagen)) ...
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bioRxiv - Pathology 2020Quote: ... The cDNA was first diluted with nuclease-free water and then added to the RT2 SYBR Green ROXTM qPCR Mastermix (Qiagen, Hilden, Germany) according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2021Quote: ... The liquid was aliquoted into a microcentrifuge tube with 590 µl nuclease-free water and 10 µl Proteinase K (QIAGEN, Hilden, Germany). For RNA extraction from swabs ...
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bioRxiv - Microbiology 2023Quote: ... where reactions were filled to 100 μl with nuclease-free water and buffer RLT from RNeasy Mini Kit (Qiagen, Venlo, the Netherlands) was added ...
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bioRxiv - Microbiology 2023Quote: ... in-house mock community (25 diverse Gram-positive and Gram-negative bacteria and fungi) and a negative reagent control (nuclease-free water, Qiagen, Hilden, Germany) were included ...
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bioRxiv - Neuroscience 2023Quote: ... 7 µL of RNase inhibitor and 98 µL nuclease-free water) and the eluted RNA was then concentrated using the RNeasy MinElute Cleanup kit (Qiagen, ref. 74204). The immunoprecipitated RNA was then reverse transcribed and used for qPCR ...
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bioRxiv - Microbiology 2022Quote: ... Total extracted RNA was resuspended in nuclease free water and one microgram was used for cDNA synthesis using the Quantitect reverse transcription kit (Qiagen, Germantown, MD, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5μl of diluted cDNA (1:3 - nuclease-free water) was used as template in 10μl real-time PCR reactions (Qiagen: ‘SYBR Green PCR kit’) to assay specific transcripts (BioRad ...
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bioRxiv - Genomics 2021Quote: ... and DNA eluted in 25 µL nuclease-free H2O (Qiagen). When required ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 50 μL of RNase-Free water (Qiagen) were applied directly on the column filter and incubated for 1 min at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μL of RNase-free water (Qiagen), and 4 μL of 1-2 ng/μL template DNA ...
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bioRxiv - Neuroscience 2021Quote: ... were mixed with RNAse–free water (Qiagen) up to 5 µL ...
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bioRxiv - Cell Biology 2024Quote: ... dissolved in RNase-Free Distilled Water (Qiagen, Cat.#10-977-015 ...
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bioRxiv - Microbiology 2019Quote: ... 8μl of Microbial DNA-Free water (Qiagen GmbH), 1.25μl of each primer (0.5mM each ...
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bioRxiv - Immunology 2020Quote: ... RNA was eluted in RNase-free water (Qiagen). The quantity and quality of mRNA were checked by nanodrop ...
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bioRxiv - Plant Biology 2024Quote: ... and resupended in 50 μl RNAse-free water (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... RNA was eluted in 50 μL RNase-Free water (Qiagen). A second DNase treatment was then performed on the samples (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was eluted in 50 μL RNase-Free water (Qiagen). A second DNase treatment was then performed on the samples (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... oligonucleotides were dissolved in DNase and RNase free water (Qiagen) and added to cultures ...
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bioRxiv - Genomics 2019Quote: ... RNA was eluted with 200 μL RNAse-free water (Qiagen) and subsequently concentrated using an Amicon Ultra-0.5 mL Centrifugal Filter with a 10 kDa cut-off ...
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bioRxiv - Molecular Biology 2021Quote: ... Each RNA sample was treated with DNA nuclease using RNAse-free DNAse kit # 79254 (QIAGEN) during RNA isolation procedure to avoid contamination by genomic DNA ...
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bioRxiv - Microbiology 2024Quote: ... The purified RNA was ultimately resuspended in RNase-Free Water (QIAGEN), and its concentration was determined at 260 nm using a NanoDrop ND-1000 spectrophotometer ...
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bioRxiv - Microbiology 2019Quote: ... Extracted DNA was eluted in 75μl Microbial DNA-Free water (Qiagen GmbH). Suitable precautions were taken during sample handling and processing in the laboratory to limit microbial contamination and maintain consistency during all procedures ...
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bioRxiv - Neuroscience 2022Quote: ... The RNA pellet was resuspended in RNAase-free water and any DNA digested using the RNase-Free DNase kit (Qiagen). RNA was eluted using the RNeasy MinElute Cleanup Kit (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... The RNA pellets were subsequently dissolved in an appropriate volume of RNase free water and treated with RNase-Free DNase (Qiagen, 79254) by incubation at room temperature for 10 min to avoid genomic DNA contamination ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were eluted in 50μl of RNase-free water supplemented with 0.04% NaN3 (Qiagen) or resuspended in RNase-free water (Trizol ...
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bioRxiv - Microbiology 2023Quote: ... The RNA samples were resuspended in RNase-free water before clean-up treatment with the RNase-Free DNase Set (Qiagen, Hilden, Germany) following the manufacturer’s protocol for off-column clean-up ...
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bioRxiv - Cell Biology 2021Quote: ... we used the sample at a 1:1000 dilution in RNAse-free water (Qiagen, 129112) (2017) ...
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bioRxiv - Bioengineering 2022Quote: ... Lysates were eluted with RNase-free water after purification using a RNeasy Mini Kit (Qiagen) and DNase I digestion ...
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bioRxiv - Microbiology 2020Quote: ... and the dilutions were performed by using Microbial DNA-Free Water (Qiagen, Valencia, CA, USA) Inoculums from all three real salmon gut compartments were diluted to 0.25 ng/μl ...
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bioRxiv - Microbiology 2019Quote: ... RNase-free water (included in the Rotor-Gene Multiplex PCR Kit, catalogue no. 204774, Qiagen, Germany), nuclease-free water (Cat ...