Labshake search
Citations for Qiagen :
551 - 600 of 1134 citations for Water Nuclease Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... DNAse treatment was performed with RNAse-free DNAse Set (Qiagen, ref: 79254). Equal volumes of all samples were reverse transcribed with Superscript IV reverse transcriptase (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... with in-column DNAse treatment using RNAase-Free DNase (Qiagen, Cat.No.79254). Sequencing libraries were prepared using SMARTer Stranded Total RNA-seq kit (Clontech Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... after on column digestion with RNase-free DNase (Qiagen, Cat No. 79256) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... including an on-column DNAse treatment using RNase-free DNase set (Qiagen) to remove genomic DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... with on-column DNase digestion (RNase-Free DNase Set, Qiagen, Hilden, Germany). Quality of the purified RNA was tested on an Agilent 2200 Tapestation using RNA screentape.
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bioRxiv - Cancer Biology 2020Quote: ... with on-column DNase digestion (RNase-Free DNase Set, Qiagen, Hilden, Germany). cDNA was prepared from 4 μg RNA using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... DNase treatment was performed immediately after total RNA purification (RNase-free; Qiagen). First strand cDNA was prepared from 400 ng of total RNA and the complementary DNA strand was synthesized using iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Physiology 2022Quote: ... the RNA samples were treated with DNAse RNAse-free (Qiagen, Hilden, Germany) and purified with Rneasy mini column (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... The plasmid was purified with the Endo-Free Mega prep kit (Qiagen) and sequenced by NGS using the approach described in Konermann et al35 ...
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bioRxiv - Plant Biology 2021Quote: ... and genomic DNA was removed by treating RNase-free DNase I (Qiagen). Extracted RNA was reverse-transcribed using oligo dT and SuperScript™ IV reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... an on-column DNase digestion with the RNase-free DNase set (Qiagen) was performed during total RNA isolation 62 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Residual DNA has been digested with the RNase-Free DNase Set (Qiagen); the abundant ribosomal RNA was depleted by using the Ribo-Zero rRNA Removal Kit (Illumina) ...
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bioRxiv - Neuroscience 2021Quote: ... and genomic DNA was digested with RNase-free DNase set (Qiagen, 79254). miRNA reverse transcription was performed using miRCURY LNA reverse transcription kit (Qiagen ...
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bioRxiv - Physiology 2021Quote: ... followed by digestion with on column-RNase-free DNAse digestion kit (Qiagen) and clean up with RNeasy mini kit (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: ... Genomic DNA was digested with DNase I (RNase-Free DNase Set, Qiagen). Concentration and purity of RNA were both evaluated with NanoDrop spectrophotometer ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 µg RNA was DNase treated using RNase-Free DNase Set (Qiagen). 1 µg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... with on-column DNase digestion with the RNase-free DNase Set (Qiagen). RNA concentration and purity were evaluated using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNeasy Mini Kit and RNase-free DNase set were from Qiagen. High-Capacity cDNA Reverse Transcription Kit was from Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... which included a genomic DNA digestion step with RNase-free DNase (Qiagen), as per the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Total RNA was isolated from the cells using the RNeasy mini isolation kit from Qiagen (Germany) with a purification step using the RNase-free DNase kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The plasmid was purified with the Endo-Free Mega prep kit (Qiagen). Of note ...
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bioRxiv - Neuroscience 2019Quote: ... Purified RNA was inbcubated with DNase I (RNase-Free DNase Set, Qiagen) over a mini kit column (RNAeasy ...
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bioRxiv - Genetics 2020Quote: ... Total RNAs were treated with amplification-grade RNase-free DNase I (Qiagen) to remove contaminating DNAs ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μg RNA was DNase treated using RNase-Free DNase Set (Qiagen) and 1 μg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: ... The cell-free extract was bound to pre-equilibrated Ni2+-NTA (Qiagen) matrix for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... with on-column DNase digestion with the RNase-Free DNase Set (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA samples were then treated with the RNase-free DNase set (Qiagen) to remove gDNA contaminants and QC checked using Qubit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... The extracted RNA was treated with RNase-free DNase set (Qiagen, USA) to remove residual genomic DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... with 15 minute on-column digestion with RNase-free DNase I (Qiagen) to eliminate genomic DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA samples were further treated with RNase-free DNase kit (Qiagen), reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: DNA-free RNA was obtained with the RNeasy MiniKit (Qiagen, Valencia, CA) with DNase treatment ...
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bioRxiv - Microbiology 2022Quote: ... Residual DNA in RNA samples was digested with RNase-free DNase (Qiagen) I and cleaned three times on a Spin Column from PureLink® RNA Mini Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... On-column DNase I treatment using the RNase-free DNase set (Qiagen) was included in the RNA extraction to remove any DNA contaminants ...
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bioRxiv - Molecular Biology 2022Quote: ... The resultant RNA was then treated with RNase-free DNase Set (QIAGEN) according to the protocol provided by the manufacturer with some minor modifications ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was removed from samples by RNase-free DNase I (Qiagen) treatment ...
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bioRxiv - Immunology 2022Quote: ... with an on-column DNase digestion using RNAse-free DNase Set (Qiagen) for 30 minutes to eliminate genomic DNA contamination ...
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bioRxiv - Neuroscience 2022Quote: ... and genomic DNA was removed using the RNase-Free DNase Set (QIAGEN). Retrotranscribed cDNA was obtained from 1 μg of total RNA using the SuperScript VILO Retrotranscription kit from Life Technologies according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... with on-column DNase digestion using RNase-Free DNase Set (QIAGEN 79254). cDNA was synthesized with random hexamers from the Superscript III First-Strand Synthesis System (Invitrogen 18080051) ...
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bioRxiv - Microbiology 2023Quote: ... frozen liquid samples using the phenol-free RNeasy Plus Mini Kit (Qiagen) according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... which included an in-line RNAse-free DNase removal step (Qiagen 79256). Total RNA was reverse transcribed to cDNA and amplified with V4-specific primers [39] ...
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bioRxiv - Immunology 2023Quote: ... DNase treatment was performed on-column using RNase-Free DNase Set (QIAGEN). cDNA was generated from 200ng-1ug RNA by using SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme Kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was removed from samples using the RNase-free DNase Set (QIAGEN). RNA quantity and quality was assessed using the Agilent 2100 Bioanalyser in conjuction with the RNA 6000 PicoLabChip Kit following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... All plasmids were purified using the Endotoxin-Free Plasmid Purification Kit (QIAGEN) prior to the in vivo gene-delivery experiment.
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bioRxiv - Cell Biology 2023Quote: ... and on-column DNase digestion with the RNAse-Free DNase Set (QIAGEN). Purity and concentration of the extracted RNA were measured using Nano-Drop lite Spectrometer (Thermo-Fisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and on-column DNA digestion with the RNase-free DNase set (Qiagen). In order to remove any remaining DNA contamination ...
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bioRxiv - Plant Biology 2023Quote: ... followed by DNase digestion with RNase free DNase Set (Qiagen, Hilden, Germany). RNA samples were quantified on Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and included DNA digestion using RNAse-free DNAse kit (Qiagen, cat.: 79254). Total RNA was quantified by UV spectrometry at 260 nm and RNA purity was estimated by 260/230 and 260/280 absorbance ratios using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... with on-column DNA digestion using RNase-Free DNase Set (Qiagen, Germany). Purified RNA was quantified using QubitTM RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... in RNase-free conditions following the user’s manual with DNase treatment (Qiagen). Quality control of the samples was done via Tapestation (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: DNA constructs were prepared using the endotoxin-free Maxiprep kit (Qiagen, #12362), following the manufacturer’s instructions ...