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Citations for Qiagen :
1 - 50 of 2439 citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 99% Buffer TCL (Qiagen, cat# 1031576), sealed with perfluorinated oil (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and eluted with 90 μL EB (Qiagen). For the respective ADT and HTO modalities ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2 μl of 10 mg/ml RNase (Qiagen Valencia, CA, USA) was added to each of the samples and kept at 4°C for 30 minutes to remove fragments of RNA strands.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... using the bead-beater homogenizer (TissueLyser LT Adapter, Qiagen; 40Hz, 90 seconds). After the lysis ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were then resuspended in 90 μl Buffer ALO (Qiagen) supplemented with 0.4X NuPAGE™ LDS Sample Buffer (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... Lung samples were homogenized with DMEM + 2% FBS in a TissueLyser (Qiagen Inc., CA) operated at 25-30 Hz for four minutes ...
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bioRxiv - Immunology 2020Quote: ... The remaining 90% of the samples were resuspended in lysis buffer (TCL Buffer (QIAGEN) supplemented with 1% 2-Mercaptoethanol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was eluted in 45 µl of EB buffer (10 mM tris-hydrochloride, pH 8.0; QIAGEN, Netherlands) supplemented with 0.05% (v/v ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from the red blood cells of the parents and 99 F2 hybrid females using the DNeasy Blood & Tissue Kit (Qiagen). The library was constructed according to a previously described method [27] ...
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bioRxiv - Microbiology 2020Quote: ... We eluted purified DNA in 45 µl of EB buffer (10 mM tris-hydrochloride (pH 8.0) (Qiagen, The Netherlands) supplemented with 0.05% (v/v ...
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bioRxiv - Genetics 2023Quote: ... Precipitated proteins were resuspended in 6M guanidine hydrochloride and incubated with 25 µL of Ni-NTA agarose beads (Qiagen) overnight ...
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bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and transferred to MaxTract High Density 2 mL tubes (Qiagen Inc, Valencia, CA, USA). Samples were then extracted a second time as described above and the aqueous phase from the repeated extractions for each sample were combined ...
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bioRxiv - Microbiology 2024Quote: ... The aqueous phase was removed and transferred to MaxTract High Density 2 mL tubes (Qiagen Inc, Valencia, CA, USA). Samples were then extracted a second time as described above and the aqueous phase from technical replicates for each soil sample were combined ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended in buffer A supplemented with 8 M guanidine hydrochloride (= buffer B) and loaded onto an Ni-NTA agarose column (QIAGEN) pre-equilibrated in the same buffer ...
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bioRxiv - Microbiology 2021Quote: ... in Tris/HCl/Ca+2 buffer was subjected to extraction in house using the silica method or QIAamp viral RNA mini kit (Qiagen) according to the manufacturer’s guidelines.
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bioRxiv - Genetics 2020Quote: ... we split the 50 μl reaction volume into two and we added 25 μl of guanidine hydrochloride (Buffer PB, Qiagen 28606) to each as a chaotropic agent to stop the reaction and dissociate the proteins and transposase from the DNA ...
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bioRxiv - Neuroscience 2024Quote: ... Inclusion bodies were solubilized in 8 M guanidine hydrochloride (GdnHCl) and then PrP was captured using Ni-NTA Superflow beads (Qiagen #30410). Bead-bound PrP was refolded on-column using a 4 h gradient from 6 to 0 M GdnHCl and then eluted using a gradient from 0 to 500 mM imidazole ...
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bioRxiv - Cell Biology 2020Quote: ... 80–90% depletion of OGT/OGA mRNAs was verified by RT-PCR using QuantiTect Reverse Transcription Kit (205311, Qiagen), QuantiTect SYBR Green PCR Kit (204143 ...
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bioRxiv - Cancer Biology 2022Quote: ... The siRNA library that contained 90 siRNA targeting 45 genes of interest was custom synthesized by Qiagen (Table S3).
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bioRxiv - Physiology 2021Quote: ... The hemolymph was diluted in 500 μL 90% (v/v) methanol, homogenized using metal beads (2.4 mm diameter, Askubal) in a tissue lyzer (Qiagen) for 1 min at 30 Hz ...
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bioRxiv - Microbiology 2022Quote: ... monocytogenes 10403S reference genome (NC_017544) and single nucleotide polymorphisms (SNPs) with a frequency of at least 90% identified using CLC workbench genomics (Qiagen) and Geneious Prime® v.2021.0.1 ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was monitored on Rotor-Gene® Q-Pure Detection System (Software Ver. 2, Qiagen Inc., Valencia, CA, USA) and performed using QuantiFast SYBR® Green PCR Master Mix (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from a SARS-CoV-2 Omicron BA.1 clinical isolate (SARS-CoV-2/human/USA/CA-CDC-4358237-001/2021) (GenBank: OM264909.1) using the QIAamp Viral RNA kit (Qiagen, Hilden, Germany). The complete wild-type Omicron genome was then reverse transcribed via RT-PCR with SuperScript™ IV First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Dissected cortex and striatum were homogenized 2 x 1min at 25 Hz in 750μL of QIAzol Lysis Reagent (Qiagen, Valencia, CA) with TissueLyser (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... HEK-beta cells were transiently transfected with WT or variant NaV1.2 (2 µg) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.).
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bioRxiv - Evolutionary Biology 2024Quote: Total genomic DNA was isolated from 2 grams of intestinal contents using the QIAamp DNA Stool Mini kit (Qiagen, Valencia, CA), following the manufacturer’s stated methodology ...
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bioRxiv - Physiology 2021Quote: ... 5 μL buffer/mg of tissue) to each sample right before mechanical lysis using the TissueLyser II (20 Hz, twice for 90 seconds) (Qiagen). Homogenates were centrifuged at +4°C (600g ...
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bioRxiv - Genomics 2022Quote: ... Amplified libraries were then purified using 1.5x ratio of SPRI beads to remove adapter dimers and fragments smaller than 90-100bp and eluted in 50 μL of EB (Qiagen) buffer after 10 minutes incubation at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the FT and Eluate fractions (90 µL each) were mixed with 10 µL 3 M sodium acetate and applied to a QIAquick spin column (Qiagen). Purified DNA was visualized a 1.3% agarose / 0.5x TBE gel and SYBR Green staining ...
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bioRxiv - Genomics 2022Quote: ... Analysis of upstream regulators of the WFB and CAF up-regulated genes was performed using Chip-seq Enrichment Analysis (ChEA3) [90] and the Upstream Regulators tab within IPA (Qiagen).
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bioRxiv - Neuroscience 2022Quote: ... blood was drawn from participants at UCSF within 90 days of clinical assessment and stored in PAXgene blood RNA tubes (Qiagen) in liquid nitrogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Digoxigenin (DIG)-labelled probes were denatured at 90°C for 4 min and diluted with 1 x microRNA ISH buffer (Qiagen), following protocol by Endisha & Kapoor ...
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from the lungs of uninfected and SARS-CoV-2 infected hamsters with or without immune suppression using Trizol reagent and purified by RNeasy mini columns (Qiagen, CA, USA) as described previously 61 ...
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bioRxiv - Microbiology 2020Quote: ... bearing a C-terminal histidine tag (ACRO Biosystems, Newark, NJ) was coated at 2 μg/ml on a Ni-NTA plate (Qiagen, Valencia, CA). After washing and blocking ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Ligated DNA fragments ranging in size from 300 to 450 base pairs (bp) were extracted from 2% low-melt agarose gels and purified using a MinElute gel extraction kit (Qiagen, Valencia, CA). The recovered fragments were amplified using PCR ...
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bioRxiv - Physiology 2022Quote: ... Genomic DNAs of 61 weaned pups (2 pups died before weaning) were collected from their tails with a DNeasy Tissue kit (Qiagen, Valencia, CA). Six Ptger3-tTA BAC transgenic founder rats were identified by PCR for the presence of the tTAad-BGH insert ...
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bioRxiv - Genetics 2024Quote: DNA was prepared from tail biopsies obtained at approximately 2-weeks of age (Gentra Puregene Mouse Tail Kit, Qiagen, Valencia, CA, USA). Scn1a genotype was determined as previously described (Kearney et al ...
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bioRxiv - Cancer Biology 2020Quote: ... were purchased from Qiagen (Qiagen, Valencia, CA) and qPCR was performed using the Qiagen miSCRIPT SYBR® Green PCR kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein in the supernatant was purified to 90% purity by a single-step chromatography on Ni++ - metal chelate affinity (Qiagen, UK) columns and eluting at 0.5 M imidazole ...
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bioRxiv - Genetics 2022Quote: ... and sex-matched controls were grown to 80-90% confluency in T75 flasks before total RNA extraction via RNeasy Plus Mini Kit (Qiagen, 74136). Whole blood samples were collected and purified using MagMAX for Stabilized Blood Tubes RNA Isolation Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA was extracted from 90% of harvested cells per flask using a Qiagen RNeasy Midi Kit (Qiagen cat. no. 75144). mRNA was isolated from 75 μg total RNA using Dynabeads Oligo(dT)25 (Invitrogen cat ...
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bioRxiv - Microbiology 2021Quote: ... transferred to the laboratory (within 2 hours) on ice and immediately processed for DNA extraction using the DNeasy PowerSoil kit (Qiagen, Valencia, CA, USA) according to manufacturer protocols ...
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bioRxiv - Microbiology 2021Quote: ... were selected with a P<0.05 and 2-fold change in gene expression and used for network/pathway analysis using Ingenuity Pathway Analysis (IPA; Qiagen, Valencia, CA, USA) as described previously (5).
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from peripheral blood leukocyte samples from visit 2 or 3 using the Gentra Puregene Blood Kit (Qiagen; Valencia, CA, USA) according to the manufacturer’s instructions (www.qiagen.com ...
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bioRxiv - Pathology 2021Quote: ... 20 – 30 mg of tissue were placed into a 2 ml tube with 600 µl of RLT buffer with 1% β-mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Valencia, CA; #69989). Tissues were then dissociated using a Qiagen TissueLyser II (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... respectively (Qiagen, Valencia, CA). Metatranscriptomic libraries were sequenced in two lanes of Illumina’s NovaSeq 6000 System flow cell utilizing the NovaSeq Xp workflow (SE 120bp ...
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bioRxiv - Molecular Biology 2024Quote: ... respectively (Qiagen, Valencia, CA). Antibodies against extracellular RNA or DNA were obtained after immunizing 4-month-old New Zealand white rabbits with nucleic acids and complete Freund’s adjuvant according to an immunization protocol described by Cold Spring Harbor Laboratories (73) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and WPMY1 parental cell lines were seeded in 6-well plates and grown for 24 hours to a confluency of 70% to 90% before RNA was extracted using an RNeasy RNA extraction kit (Qiagen, Venlo, the Netherlands). Samples were prepared in triplicate ...
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bioRxiv - Neuroscience 2023Quote: ... 10% of the cells were used for passaging and DNA was extracted on the remaining 90% of the cells using a Qiagen DNA extraction kit (Qiagen; Cat. No. 69504). Clones were screened by PCR (DLK KO primers ...
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bioRxiv - Neuroscience 2022Quote: ... with TissueLyser (Qiagen, Valencia, CA) and 5mm stainless steel beads (Qiagen ...