Labshake search
Citations for Qiagen :
51 - 100 of 5229 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2021Quote: ... The DNA was purified by agarose gel electrophoresis and extracted using the Qiagen gel extraction kit (Qiagen), followed by quantification by q-PCR (Brilliant III Ultra-Fast SYBR® QPCR ...
-
bioRxiv - Biochemistry 2023Quote: ... The PCR product was confirmed for size by agarose gel electrophoresis and subsequently purified by QIAquick (Qiagen) columns ...
-
bioRxiv - Microbiology 2021Quote: ... This was purified by 1% agarose gel electrophoresis and gel extracted according to QIAquick gel extraction kit (QIAGEN) standard protocol ...
-
bioRxiv - Microbiology 2019Quote: ... Successful amplification was confirmed by gel electrophoresis before samples were cleaned using the MinElute PCR Purification kit (Qiagen). PCR products were quantified and ∼80 ng of dsDNA was carried forward to library preparation using the NEBNext Ultra DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs) ...
-
bioRxiv - Genetics 2021Quote: ... An approximately 1500bp PCR product was isolated using agarose gel electrophoresis and purified using QIAprep (QIAGEN, Valencia, CA). Sanger-sequencing was performed by Laragen (Culver City ...
-
bioRxiv - Cell Biology 2021Quote: ... The PCR #2 products were validated using gel electrophoresis and purified using the QIAquick Gel extraction kit (QIAGEN). The purified libraries were then quantified by Nanodrop and Qubit prior to sequencing on an Illumina MiSeq.
-
bioRxiv - Molecular Biology 2021Quote: ... Amplification products were analyzed by electrophoresis and were purified using the QIAquick PCR purification kit (Qiagen, Germantown, MD) according to the manufacturer’s protocol ...
-
bioRxiv - Paleontology 2022Quote: ... pools were blended at equimolar amounts based on DNA concentration readings from a QIAxcel capillary electrophoresis device (Qiagen). Lastly ...
-
bioRxiv - Genomics 2019Quote: ... The sequences were purified by agarose gel electrophoresis and the QIAEX II Gel Extraction Kit (Qiagen, Hilden, Germany).
-
bioRxiv - Genetics 2021Quote: Long PCR products were purified by gel electrophoresis and short ones with the QIAquick PCR purification kit (Qiagen). Terminal adenine overhangs were added using Taq polymerase in the presence of dATP ...
-
bioRxiv - Systems Biology 2021Quote: ... Samples were purified by DNA agarose gel electrophoresis followed by gel extraction using QIAquick Gel Extraction Kit (Qiagen).
-
bioRxiv - Molecular Biology 2021Quote: ... Adapter-ligated samples were purified by agarose gel electrophoresis and extraction with the QIAquick Gel Extraction kit (Qiagen) according to manufacturer’s protocol ...
-
Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Size was validated by agarose gel electrophoresis and PCR products were purified using QiaQuick PCR Purification Kit (Qiagen). Equimolar amounts of purified DNA fragments (minimum 15fmol ...
-
bioRxiv - Cancer Biology 2023Quote: ... The PCR #2 products were validated using gel electrophoresis and purified using the QIAquick Gel extraction kit (QIAGEN). The purified libraries were then quantified by Nanodrop and Qubit prior to sequencing on an Illumina MiSeq (2 samples per lane for >100X coverage).
-
bioRxiv - Plant Biology 2024Quote: ... All fractions were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using anti-His (Qiagen) and anti-strep (MilliporeSigma ...
-
bioRxiv - Genetics 2021Quote: ... PCR 2 products were purified by electrophoresis with a 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen) and eluting with 30 μL water ...
-
bioRxiv - Genomics 2020Quote: ... Successful amplification was confirmed using 1.5% agarose gel electrophoresis before purifying second PCR amplicons with the MinElute Purification kit (Qiagen). The next step was quantifying purified samples with a QuantIT PicoGreen daDNA assay kit and using these values to normalize all samples to 3 ng/μL ...
-
bioRxiv - Molecular Biology 2020Quote: ... The products of restriction enzyme digests were purified by preparative gel electrophoresis followed by QIAquick gel extraction kit (Qiagen). Restriction endonucleases ...
-
bioRxiv - Genomics 2021Quote: ... The amplified fragments were separated by agarose-gel electrophoresis and purified using QIAquick Gel Extraction Kit (QIAGEN, Limburg, Neferland). DNA concentrations were measured using a droplet-based absorptiometer ...
-
bioRxiv - Biochemistry 2022Quote: ... PCR products were verified and purified by standard agarose gel electrophoresis methods utilizing DNA sample clean up kits (Qiagen), and were sequenced using the same primers through commercial services (Source Bioscience ...
-
bioRxiv - Microbiology 2021Quote: ... PCR products were confirmed by agarose gel electrophoresis using 0.8-1% agarose and purified using PCR purification kit (Qiagen). In case of NS1 gene ...
-
bioRxiv - Synthetic Biology 2019Quote: ... PCR amplicons were then resolved by gel electrophoresis followed by DNA isolation with a QIAquick Gel Extraction Kit (Qiagen). The amplicons were Sanger sequenced (Eurofins Genomics ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The 202 bp PCR product was purified by electrophoresis in agarose gel using a QIAquick Gel Extraction Kit (Qiagen), and eluted in 43 μL of water ...
-
bioRxiv - Genomics 2021Quote: ... PCR 2 products (pooled by common amplicons) were purified by electrophoresis with a 1.5% agarose gel using a QIAquick Gel Extraction Kit (Qiagen), eluting with 40 µL of water ...
-
bioRxiv - Microbiology 2022Quote: ... Successful amplification and correct size of the sul1 and intI1 PCR produces were finally confirmed by 1.5% agarose gel electrophoresis (40 min, 110V) and subsequently purified with the QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2023Quote: ... DNA fragments resolved with electrophoresis of 1.2% Agarose gel were exercised and purified using the Gel Extraction kit (Qiagen) and subject to Sanger sequencing service provided by the Psomagen Service Center (New York).
-
bioRxiv - Plant Biology 2023Quote: ... and fragments 300–1000 bp in length were fractionated by electrophoresis in 2.0% agarose gel (Kanto Chemical Co., Inc., Tokyo, Japan) with a MinElute Gel Extraction Kit (Qiagen). These libraries were sequenced on a HiSeq 4000 sequencer (Illumina Inc. ...
-
bioRxiv - Cell Biology 2023Quote: ... The products of restriction enzyme digests were purified by preparative gel electrophoresis followed by QIAquick Gel Extraction Kit (Qiagen). Ligation were performed with T4 DNA ligase (New England Biolabs ...
-
bioRxiv - Developmental Biology 2023Quote: ... PCR products were purified using gel electrophoresis and gel purification using a QiAquick gel extraction kit (Qiagen, Cat. #28704). Purified PCR products were sent for sanger sequencing using the same primers as used in the RCR amplification step ...
-
bioRxiv - Bioengineering 2023Quote: ... and the desired DNA fragment was separated by gel electrophoresis and extracted using gel extraction kit (Qiagen, Hilden, Germany). The extracted DNA was further treated with T4 DNA polymerase (Takara Bio Inc. ...
-
bioRxiv - Bioengineering 2023Quote: ... PCR products were extracted from agarose gels after electrophoresis and purified using the QIAEX II Gel Extraction Kits (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Products were separated by agarose gel electrophoresis and library amplicons were extracted using the QIAquick Gel Extraction Kit (Qiagen). Amplicons were quantified with the KAPA Library Quantification Kit for Illumina Platforms (KAPA Biosystems ...
-
bioRxiv - Cancer Biology 2023Quote: ... Amplified PCR products (∼240–250 bp) were purified by agarose gel electrophoresis using the QIAquick gel extraction kit (Qiagen). Purified PCR products were quantified using the Qubit dsDNA high sensitivity assay kit (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... the amplified gene fragments of the VH and Vκ/Vλ were each subjected to electrophoresis on a 1.5% agarose gel and purified via DNA size using the QIAquick gel extraction kit (QIAGEN) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... insertion of the gRNA was confirmed by gel electrophoresis of PCR products and the plasmid was purified using the QiaQuick Gel Extraction kit (Qiagen) following provider’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... The PCR products were detected by gel electrophoresis and purified with the MinElute PCR Purification Kit (Qiagen, Valencia, CA, USA) and sequenced.
-
bioRxiv - Genomics 2020Quote: PCR amplicons verified by 1% agarose gel electrophoresis were purified from gel using Gel extraction kit (Qiagen GmbH, Hilden, Germany) and ligated into a pGEM-T easy cloning vector (Promega ...
-
bioRxiv - Molecular Biology 2020Quote: ... The PCR products were also run on a QIAxcel capillary gel electrophoresis system according to the manufacturer’s instructions (Qiagen, Germany) and fragments were sized and scored using QIAxcel ScreenGel software (Qiagen ...
-
bioRxiv - Genetics 2019Quote: ... purified using an E-gel electrophoresis system (Agarose 2%) followed by column purification with a MinElute PCR Purification Kit (Qiagen). In order to introduce the doped sequence in the full-length TDP-43 sequence the purified oligonucleotide was cloned into 100 ug of linearized pRS416 Gal TDP-43 by a Gibson approach (Supplementary Table 1 ...
-
bioRxiv - Cancer Biology 2019Quote: ... the amplified sgRNA barcode PCR product (∼240-250 bp) was purified by agarose gel electrophoresis using the QIAquick gel extraction kit (Qiagen). The concentration of individual libraries was quantified by fluorescence using the Qubit dsDNA high sensitivity assay kit (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2021Quote: ... The PCR products were subjected to 1 % agarose gel electrophoresis for analysis and purified with QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2021Quote: ... PCR reactions were analysed by agarose gel electrophoresis and PCR products of the predicted size were extracted from the gel using QIAquick Gel Extraction Kit (Qiagen). Fragments were ligated into the pCR8/TOPO Gateway entry vector (Invitrogen) ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR products were isolated by agarose gel electrophoresis and subsequent gel extraction using the QIAquick Gel Extraction Kit (Qiagen). The purified PCR products then were processed and sequenced ...
-
bioRxiv - Microbiology 2021Quote: ... The PCR products were isolated by agarose gel electrophoresis and subsequent gel extraction using the QIAquick Gel Extraction Kit (Qiagen). The purified PCR products were then processed and sequenced using the NextSeq 75 – High Output (82 cycles in read 1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Correct and unique amplification of the target regions was verified by agarose gel electrophoresis before purifying PCR products using the QIAquick PCR Purification Kit (Qiagen). For analysis by TIDE ...
-
bioRxiv - Genomics 2020Quote: ... followed by evaluation of the PCR products by gel electrophoresis and purification with the Qiaquick PCR purification kit (28104, Qiagen). These PCR primers were evaluated for specificity in human (MCF7 ...
-
bioRxiv - Genomics 2022Quote: PCR amplicons verified by 1% agarose gel electrophoresis were purified from gel using Gel extraction kit (Qiagen GmbH, Hilden, Germany) and ligated into a pGEM-T easy cloning vector (Promega ...
-
bioRxiv - Neuroscience 2019Quote: ... Library fragments in the range of 100-800 bp were size-selected using agarose gel electrophoresis followed by DNA gel extraction (QIAGEN). Recovered DNA was further cleaned and concentrated using a column (Zymo Research) ...
-
Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: ... The products of restriction enzyme digests were extracted and purified by preparative gel electrophoresis followed by QIAquick gel extraction kit (Qiagen). Restriction endonucleases ...
-
bioRxiv - Biochemistry 2021Quote: ... The CYP168A1 optimized cDNA insert was removed from the plasmid using NdeI and HindIII restriction enzymes and isolated using agarose gel electrophoresis and the Qiagen Gel Extraction Kit (Qiagen) for ligation into a similarly digested and isolated pCWOri+ CYP expression vector (113) ...