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Citations for Qiagen :
551 - 600 of 5229 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Additional sequencing performed on gDNA extracted by QIAGEN Blood & Cell Culture DNA Maxi Kit (QIAGEN, 13362), followed by PCR and insertion into Zero Blunt TOPO PCR Cloning Plasmid (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted in RTL Buffer Plus (Qiagen) containing 1% ß-mercaptoethanol (Sigma ...
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bioRxiv - Neuroscience 2024Quote: RNA was isolated from cell lysates (Qiagen, 74106) and reverse transcribed to generate cDNA (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: Cells were sorted into TCL lysis buffer (Qiagen) before extraction of RNA using RNAClean XP beads (Beckman) ...
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bioRxiv - Biochemistry 2023Quote: ... brucei cells using the RNeasy mini kit (Qiagen). RNA was treated with Precision DNase Kits (Primer Design ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed using QIAzol lysis kit (Qiagen) and RNA was extracted using RNAeasy mini kit (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... cultured cells were collected in RNeasy (Qiagen, Inc.) for RNA purification ...
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bioRxiv - Microbiology 2024Quote: ... the cell pellet was treated with RNAProtect (Qiagen) and RNA was isolated with Qiagen RNAEasy (#74104) ...
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bioRxiv - Genomics 2024Quote: Cells were lysed with Buffer RLT Plus (Qiagen) containing 1% beta-mercaptoethanol (Sigma) ...
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bioRxiv - Genomics 2024Quote: ... cells were lysed with buffer RLT Plus (Qiagen) containing 1% beta-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: Cells were sorted into RLT lysis buffer (Qiagen) with 1% β-mercaptoethanol ...
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bioRxiv - Immunology 2024Quote: ... cells pelleted and lysed in RLT buffer (Qiagen). RNA was extracted using Qiagen RNeasy micro kit and their transcriptome measured using the nanostring Human Immunology V2 characterisation panel (Nanostring technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected using Effectene transfection reagent (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA from 60 million unsorted cells (500× coverage of the GeCKO v2 library) and 6 million sorted cells was isolated using Blood & Cell Culture DNA Midi Kit (QIAGEN, 13343) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... total RNA was extracted from cells from stable-cell lines created using a RNeasy Plus Mini Kit (Qiagen, catalog number: 74134) following the Manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were then rinsed with 1X PBS and processed for subcellular fractionation (Qproteome Cell Compartment Kit, Qiagen; following manufacturer’s protocol). Nuclear fractions were isolated and protein precipitated by incubation in 4x volume of ice-cold acetone for 15 minutes followed by pelleting (10 min ...
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bioRxiv - Microbiology 2020Quote: Total cellular DNA was extracted from cells (cell lines or PBMCs) using a QIAmp DNA Blood mini kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from untreated cells (24 h) and IFNγ-treated cells (6 or 24 h) were extracted with RNeasy plus mini kit (Qiagen, 74134) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... At the end of activation cells were washed twice with PBS and cell pellets were snap-frozen in RLT Plus lysis buffer (Qiagen, #1053393). Total RNA was extracted with the Qiagen’s RNeasy kit according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted for each of the persistently infected cells as well as the cell lines without NDV infection (negative control) using Qiagen RNeasy micro kit (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysis and RNA extraction from siRNA transfected cells were performed using the RNeasy RNA extraction kit (Qiagen Inc, Germantown, MD). 1 µg of total RNA was reverse transcribed with the High-capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: E3 and E4 primary microglia were plated at 5×106 cells/well in 6-well plates and RNA was extracted from the cells using the RNEasy Plus Mini Kit (Qiagen #74136) and converted to cDNA using High-Capacity RNA-to-cDNA kit (Thermo #4387406 ...
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bioRxiv - Genomics 2019Quote: ... and the remaining cells in single-cell capture (T1, T2, and T3 samples) using the QIAamp DNA Mini kit (Qiagen, 51304), and 500 ng of DNA were used to construct exome sequencing libraries with the same reagents as the single cell DNA-seq.
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bioRxiv - Genomics 2021Quote: ... the cells were collected and total RNA was extracted from the cells using the Qiagen RNeasy® Plus Kit (Qiagen, 74134). One µg of RNA was then used to synthesize cDNA using the Bio-RAD iScript cDNA Synthesis Kit (Bio-RAD ...
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bioRxiv - Cell Biology 2019Quote: ... MCMBP depleted cells using siRNA and MCMBP-KO (clone #1 and #2) cells was isolated using RNeasy Mini Kit (Qiagen, 74104). The cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Genetics 2021Quote: ... S2 cells were seeded at 1 × 106 cells/ml in a 6-well plate and transfected using Effectene (Qiagen, Germantown, MD). For mobility shift assay (Figure 4) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A pellet of 200-400 µl of animals was resuspended in 400 µl Cell Lysis Solution (Qiagen Gentra Puregen Cell kit) with 5 µl proteinase K (20 mg/ml ...
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bioRxiv - Evolutionary Biology 2022Quote: ... log-phase cells in the range of 1–2 × 108 cells using the Qiagen DNeasy® Blood and Tissue kit (Qiagen). We resuspended the genomic DNA in 10 mM Tris-HCl pH 8.5 and stored it at 4° until submission to the McDonnell Genome Institute at Washington University in St ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from 1,000 H3K27me3 HI/LO β-cells or from 50,000 sorted βHI and βLO cells was extracted using the miRNeasy FFPE Kit (QIAGEN, 217504), followed by the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (E6420L) ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from CD33+ ITGA7-sorted primary AML cells (> 4x105 cells/sample) using the RNeasy Mini kit (Qiagen, 74106) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNAlater was removed from the sorted cells prior to RNA isolation and total RNA was isolated from the pelleted cells using the RNeasy mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions with some modifications ...
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bioRxiv - Microbiology 2020Quote: ... RNA from cell lysates or cell culture supernatants was extracted using the Qiagen QIAamp viral RNA mini kit (Qiagen, Valencia, CA, USA). Reverse transcription (RT ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA and DNA was extracted from the same cells from neuropathologist annotated tumour regions (>60% cancer cells) using appropriate Qiagen kits (Qiagen, Sussex, UK). Paired end strand-specific whole transcriptome libraries were prepared for 16 pairs using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Cancer Biology 2020Quote: DNA coming from CD34+ cells of the patient and Mononuclear cells of Healthy Donors was extracted by means of DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). 50ng of DNA were analysed through P056 MLPA kits (MRC Holland ...
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bioRxiv - Cell Biology 2021Quote: ... Genomic DNA was extracted from the control and the peptide-treated frozen cells using the Blood & Cell Culture DNA Midi Kit according to manufacturer’s instructions (Qiagen, ref. no. 13343). A first PCR was performed to amplify the lentiCRISPR sgRNA region using the following primers:
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA was extracted from osimertinib-resistant cells as well as matched parental cells were extracted using a DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). We reviewed all candidate alterations identified in resistant cells as well as parental cells and considered those identified only in resistant cells as candidate acquired resistance genomic alterations to osimertinib.
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bioRxiv - Immunology 2021Quote: DNA was extracted from the cultured cells or ex vivo CD4+ T cell samples using DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from purified CD4+ TN and TCM cells and from 24ST1NLESG cells using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... gDNA was isolated from 5 x 106 Colo205 cells using the Blood and Cell Culture DNA Maxi Kit (Qiagen, catalog no. 13362) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Suspension of lysed cells or tissues in RLT buffer was defrosted and cells were disrupted and homogenized using the QIAshredder system (Qiagen, cat. 79656) and total RNA was then extracted with the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: gDNA samples from the unselected control (gDNACtrl) and 2nd (gDNAScreen) selected group cells were extracted using the Blood and Cell Culture DNA Midi Kit (#13343; QIAGEN, Germantown, MD).
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from the SBMA OL cell model and the control cell model using the RNeasy Mini Kit (Qiagen, Hilden, Germany). The quality and quantity of the RNA obtained were checked using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was extracted from sorted cells (RNeasy, Qiagen) and stored at −80°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... washed with 1 ml RNAprotect® Cell Reagent (Qiagen) and centrifuged for 10 min at 2,500 x g ...
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bioRxiv - Immunology 2022Quote: Respective cell populations were lysed in RLT buffer (Qiagen) containing 2-mercaptoethanol or TRIzol (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... cells were lysed in RLT buffer (Qiagen, Hilden, Germany) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Immunology 2022Quote: ... cells were suspended in RLT buffer (Qiagen, Valencia, CA), homogenized ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from cell lysates (Qiagen RNeasy kit) and IFIT1 expression was measured by qRT-PCR (IDT Assay ID Hs.PT.561.20769090.g).
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bioRxiv - Genomics 2019Quote: ... Isolated PBMCs were resuspended in RNAprotect cell reagent (Qiagen) and stored at −80 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were reverse transfected with LNA GapmeRs (Exiqon, QIAGEN) at a final concentration of 50 or 100 nM (with similar results) ...