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Citations for Qiagen :
1 - 50 of 1144 citations for Tetanus Toxoid Recombinant Heavy Chain Fragment C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... plasmids bearing chimeric antibody heavy and light chain genes were prepared (EndoFree Plasmid Mega prep Kit, QIAGEN) and transfected into Human Embryonic Kidney cells (HEK293F ...
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bioRxiv - Biochemistry 2020Quote: ... low melting point agarose gel and the approximately 700 base pair band corresponding to the heavy chain only immunoglobulin was extracted and purified using QIAquick Gel Extraction Kit (Qiagen). A secondary amplification of the VHH domain was performed using modified primers (VHH-Esp-For ...
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bioRxiv - Cell Biology 2022Quote: ... Drosophila S2 cells were transfected with mNeonGreen-tagged kinesin-1 full-length heavy chain (KHC) and Halo-tagged PLP584-1811 (Effectene, QIAGEN). The lysates were harvested for TIRF motility assays at ∼48 h post-transfection ...
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bioRxiv - Immunology 2020Quote: ... cDNA products were then used in nested PCR for heavy/light chain variable region amplification with HotStarTaq Plus DNA Polymerase (QIAGEN # 203643) and specific primer sets described previously 34,35 ...
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bioRxiv - Molecular Biology 2021Quote: ... Approximately 700 base pair bands corresponding to the heavy-chain only immunoglobulin were extracted (QIAquick Gel Extraction Kit: Qiagen K.K., Tokyo, Japan). Nested PCR was performed to amplify coding genes of VHH domains using the VHH-PstI-For and VHH-BstEII-Rev primers and subcloned into the pMES4 phagemid vector5 ...
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bioRxiv - Cancer Biology 2023Quote: ... and lower bands corresponding to the heavy-chain only immunoglobulin were extracted using the QIAquick Gel Extraction Kit (Qiagen K.K., Tokyo, Japan). Subsequent nested PCR was performed to amplify coding genes of VHH domains using the VHH-PstI-For and VHH-BstEII-Rev primers and subcloned into the pMES4 phagemid vector36 ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments that were generated by the polymerase chain reaction (PCR) were purified using the PCR Purification Kit (Qiagen), Monarch® PCR & DNA Cleanup Kit (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant both N-and C-terminally His-tagged proteins were purified using a Ni-NTA Superflow cartridge (1ml, Qiagen) and the Fast Protein Liquid Chromatography (FPLC ...
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bioRxiv - Immunology 2021Quote: Conditioned medium of HEK293 cells producing recombinant sema3A fused with 6xHistidine tag in C-terminal was collected and purified using Ni-NTA agarose beads (QIAGEN). The protein activity was assessed using the cytoskeleton collapse assay (19 ...
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bioRxiv - Cell Biology 2023Quote: ... This fragment (fragment 1) was gel-purified (QIAquick DNA cleanup, Qiagen) to remove any residual plasmid and contain only a linear template.
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bioRxiv - Cancer Biology 2022Quote: ... The cleaved fragments were released by incubating the tube for 30□min at 37□°C and then purified by Qiagen MinElute PCR Purification Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated at 37 °C for 30 min followed by immediate purification of DNA fragments with the MinElute PCR Purification Kit (Qiagen). ATAC-seq including amplification of Library and Indexing was performed as described elsewhere67 ...
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bioRxiv - Microbiology 2020Quote: ... Fragments were purified (Qiagen) and Gibson Assembly was performed as suggested by the manufacturer and transformed into E ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragments were purified using a MinElute PCR fragment purification kit (Qiagen). Real-time PCR reactions were performed using the KAPA probe fast universal master mix and the indicated primers on a Rotorgene 6’000 machine ...
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bioRxiv - Microbiology 2023Quote: ... we dried both washed lichen fragments and wash-water pellets at 65°C and extracted DNA with the DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... and AR DBD domain DNA fragment were cut by NheI (N-terminal) and XhoI (C-terminal) and purified with the QIAquick Gel Extraction Kit (Qiagen; cat. 28704). After ligation with T4 DNA Ligase (New England ...
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bioRxiv - Microbiology 2023Quote: ... Polymerase chain reaction (PCR) was performed with the HotStarTaq Plus polymerase (Qiagen) with 5 min initial denaturation at 94°C ...
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bioRxiv - Microbiology 2020Quote: Polymerase chain reaction (PCR) was performed using Taq DNA Polymerase (QIAGEN, Hilden, Germany). The fungal ITS-28S rRNA gene region was obtained using the ITS5 (White et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Purification of His6-light chain TeTx was done using Ni-NTA resin (Qiagen) in 50 mM Tris HCl pH 7.4 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... QuantiTect Probe RT-PCR (reverse transcription polymerase chain reaction) Kit (QIAGEN, Hilden, Germany) was used to perform RRT-PCR reactions in the ABI 7300 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2023Quote: Polymerase Chain Reactions (PCRs) were performed using Type-it Microsatellite PCR Kit (Qiagen) in 10 µl reactions ...
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bioRxiv - Immunology 2022Quote: ... Recombinant plasmids were then isolated (MiniPrep, Qiagen) and sequenced (DNA Sequencing Facility ...
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bioRxiv - Neuroscience 2021Quote: ... DNA fragments were purified by Qiagen MinElute PCR Purification Kit from the supernatant and used for the construction of sequencing libraries ...
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bioRxiv - Microbiology 2020Quote: ... This fragment was PCR purified (Qiagen) and ligated into HindIII digested gel-purified pHERD20T (37 ...
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bioRxiv - Microbiology 2019Quote: ... Fragments were stored in RNAlater (Qiagen) at room temperature and transported to the laboratory for DNA extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR fragments were column purified (Qiagen) and quantified ...
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bioRxiv - Immunology 2023Quote: ... Digested DNA fragments were purified (Qiagen) and ligated to gel-purified plasmid backbones using T4 DNA ligase (Roche) ...
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bioRxiv - Physiology 2023Quote: ... and real-time polymerase chain reaction (PCR) was performed with Fast SYBR Green (QIAGEN). Gapdh was used as the housekeeping gene ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were grown to approximately 80% confluency in light and heavy media and transfected using Attractene (#301007, Qiagen, Germany) with SBP-empty vector (control cells ...
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bioRxiv - Immunology 2019Quote: ... Input material (total cytoplasmic mRNA) and efficiently translated mRNA (heavy polysome-associated, >3 ribosomes) were extracted with QIAzol (Qiagen) and purified using RNeasy MinElute Cleanup Kit (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... PCR-amplified fragments and digested fragments separated on 0.8% agarose gels were purified with kits from Qiagen (France). Restriction enzymes ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR fragments were purified from gels (Qiagen), their concentration measured using a BioPhotometer D30 (Eppendorf ...
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bioRxiv - Cell Biology 2021Quote: ... The subsequent fragment was gel purified (Qiagen) and ligated using T4 ligase (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... fragments were purified using MinElute columns (Qiagen). ATAC-seq libraries were amplified by 10 PCR cycles using the Nextera index system (Illumina) ...
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bioRxiv - Genetics 2020Quote: ... Transposed DNA fragments were purified by Qiagen MiniElute PCR purification kit (Qiagen) ...
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bioRxiv - Physiology 2020Quote: ... The polymerase chain reaction (PCR) were carried out using Rotor Gene Q (Qiagen, Hilden, Germany) with initial incubation at 95 °C for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction was conducted using miRCURY LNA miRNA SYBR PCR kit (Qiagen #339345) as per manufacturer’s instruction on BioRad CFX96™ System in 96-well plates in 3-6 repeats ...
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bioRxiv - Molecular Biology 2021Quote: Polymerase chain reaction (PCR) products were gel purified using Qiagen QlAquick gel purification columns (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The TEV-MCS fragment and BamHI + KpnI digested EGFP fragment was ligated into BamHI + SalI digested pQE30 vector (Qiagen) in a 3-fragment ligation reaction ...
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bioRxiv - Plant Biology 2021Quote: ... recombinant protein and purified using Ni-NTA resin (Qiagen). Polyclonal antibodies were raised in mice as described in 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant protein was purified using Ni-NTA agarose (Qiagen). Rabbit polyclonal antibodies were generated by Covance ...
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bioRxiv - Microbiology 2023Quote: ... The two fragments of yfhO and the fragment with the kanamycin resistance cassette were purified using the QIAquick PCR purification kit (Qiagen) and fused by Gibson Assembly (SGA-DNA ...
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bioRxiv - Microbiology 2019Quote: ... DNA fragments were purified using MiniElute columns (Qiagen) and quantified using the Qubit dsDNA HS kit (Life Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... and form frozen tissue fragments - using QIAzol (Qiagen), RNA extraction beads (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA fragments were purified using Qiaquick minielute (Qiagen) according to the manufacturer protocol and diluted to 1/10 for input and to the half for immunoprecipitated fractions ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested PCR fragments were gel-purified (Qiagen #28104) and ligated into the pLVX-Puro vector (Takara #632164 ...
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bioRxiv - Genomics 2023Quote: ... The amplified fragments were gel purified (QIAGEN 28506) and shuttled into pDONR221 (ThermoFisher 12536017 ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA fragments were purified using MinElute columns (Qiagen). Libraries were prepared using NEBNext Ultra II DNA Library prep kit (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time polymerase chain reactions were performed using the QuantiFast SYBR Green PCR Kit (QIAGEN) and the CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Transgenic animals were identified by polymerase chain reaction of tail DNA (DNeasy kit, Qiagen, Hilden, Germany) using primers for Cre (Schönig et al. ...