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Citations for Qiagen :
551 - 600 of 5289 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then harvested and subjected for genomic DNA isolation using “Blood & Cell Culture DNA Mini Kit” (Qiagen, Hilden, Germany). Reactivation of LCLs was determined by real-time quantitative PCR (qPCR ...
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bioRxiv - Immunology 2019Quote: DNA and RNA were extracted from cells using either Blood & Cell Culture DNA Mini Kit and RNAeasy isolation kit (Qiagen), or all-prep DNA/RNA kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: RNA from isolated fresh B cells and B cells activated with LPS/IL-4 was extracted using a RNeasy kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from wild-type and ΔTMPRSS2 293T cell lines using the Puregene cell kit according to the manufacturer’s instructions (Qiagen, #158388). Co-transfection of pJC144 (guide 1 ...
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bioRxiv - Microbiology 2020Quote: ... or experimental siRNA 72 h after transfection (approximate cell concentration was 1.6×105 cells/ well) were harvested and lyzed using the QIAshredder Kit (Qiagen; 79656). mRNA was harvested using the RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... was extracted from the top (5.61 million cells) and bottom (4.87 million cells) fractions using the QIAamp DNA mini kit (QIAGEN cat.#51304). Fixed cells were decrosslinked by overnight incubation in 200 µL of PBS with added Proteinase K and 200 µL buffer AL (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: RNAs were prepared from purified D0 cells and from D7 to D14 populations isolated by flow cytometric cell sorting using an RNeasy Plus kit (Qiagen), and then depleted of rRNAs and processed for RNA-Seq library construction using a TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: ... gDNA was isolated from at oncogene-negative mouse cell lines or FACS-sorted Tomatopositive cancer cells using either the AllPrep DNA/RNA(Qiagen) or the DNeasy Blood and Tissue Kit ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and CD45+ Calcein+ DAPI-CD19+ CD38+ activated B cells were single-cell sorted into 96-well plates with catching buffer (RLT lysis buffer (Qiagen) with 1% 2-mercaptoethanol (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were removed and the remaining cell pellet (500 μl) was transferred into a 15 ml tube containing 2.5 ml RNAprotect® Cell Reagent (Qiagen). The cells were resuspended by vortexing ...
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bioRxiv - Microbiology 2021Quote: Total RNA (5×106 cells) was extracted from FEA or CRFK cell lines by using the RNeasy Plus Mini Kit (Qiagen). RNA from FEA ...
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bioRxiv - Immunology 2021Quote: Human B cells harvested post gene-editing or cell sorting were lysed for RNA extraction by the RNeasy Micro Kit (74004, Qiagen). Primers used for reverse transcription and library amplification were modified from previous29 ...
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bioRxiv - Genomics 2021Quote: ... DNA was isolated from thawed APOE cell lines by Bioneer A/S using the Blood & Cell Culture DNA Mini Kit (Qiagen). For analysis of Pax8-CreERT2 insert high molecular DNA was prepared from mouse ears using MagAttract HMW DNA Kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA (gDNA) isolation from bulk transfected cells and clonal cell lines was performed using the DNeasy Blood and Tissue kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from the FACS-purified cells was isolated immediately after FACS sorting using Qiagen RNA Mini (>100,000 cells, Qiagen 74104) or RNA Micro kits (<100,000 cells ...
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bioRxiv - Microbiology 2022Quote: RNA was isolated from approximately 107 cells grown to a density of 106 cells/ml using the RNAeasy Mini Kit (Qiagen), including an on-column DNase I treatment (Qiagen) ...
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bioRxiv - Neuroscience 2022Quote: ... iPS-NES cells were plated in 6-well plates and lysates were collected in RNAprotect® Cell Reagent (QIAGEN, #76526) to stabilize the RNA ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 5 million cells per cell line using the Qiagen AllPrep DNA/RNA extraction kits (Qiagen, 80004). Nucleic acid quantification and quality detection were performed and RNA sequencing libraries were generated via Illumina Stranded mRNA prep ...
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bioRxiv - Microbiology 2022Quote: DNA was purified for sequencing from cells infected in six well plates using a blood and cell culture DNA mini kit (Qiagen). Samples were sequenced at the Northwestern University Genomics Core Facility using NextGen Illumina HiSeq SR500 sequencing ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was extracted from puromycin selected SUDHL4 cells and untreated WT cells using the QIAamp DNA Blood Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Purified CD4+FYP+ Treg cells were stimulated with Cell Stimulation Cocktail (eBioscience) for 2 hrs and lysed in RLT buffer (Qiagen) containing 1% 2-mercaptoethanol (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... the cell culture supernatants were collected and cells were lysed using RLT Buffer from the RNeasy Mini Kit (#74106; Qiagen).
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from 1 million cells of each cell line using the QIAamp®DNA Micro Kit (QIAGEN) following the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondria isolation from HEK293T cells was performed after 24 hours of cell transfection using the Q proteome Mitochondria Isolation Kit (QIAGEN). Cell lysis and mitochondria homogenisation were conducted as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Investigation of the cell cycle related genes in LoVo cell line on treatment with FA FOS I was performed as described above by using the synchronized and 24 h FA FOS I (198 µg/ml) treated cells profiled by using RT2 Prolifer PCR Array- Human Cell cycle from Qiagen. The data was analysed by using Gene globe platform and reactome pathway analysis.
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bioRxiv - Systems Biology 2023Quote: ... DAPT was washed out and cells were allowed to signal for 6 hours before cells were harvested for RNA extraction using QIAshredder columns (Qiagen) and the RNeasy Mini Kit per the manufacturer’s instructions (see next section for further details of the plated ligand assay) ...
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bioRxiv - Molecular Biology 2023Quote: Cells collected from either 3D culture organoids or sorted cells were lysed in RLT buffer from RNeasy kit (Qiagen, 74106). RNA samples were isolated using the same kit following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... phenol-free total RNA was extracted from cells directly in cell culture wells using RNeasy Plus Micro Kit with gDNA eliminator columns (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA from Library-Diverse was isolated by Blood & Cell Culture DNA Maxi Kit (Qiagen; 30 million cells per condition). Genomic DNA from the TRIP library editing experiments was isolated by DNeasy Blood & Tissue Kit (4 million cells per condition) ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA Extraction and Purification: Genomic DNA was extracted from 5 M cells using the Gentra Puregene Cell Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Hybridoma cells were lysed by resuspending the cell pellet in 150 µL of RNeasy Lysis Buffer (RLT) (Qiagen Catalog# 1015750) and transferred to the corresponding wells of a 96 well U bottom master block plate (Greiner Catalog# 780215) ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA from freshly sorted single cells was extracted and amplified using the REPLI-g Advanced Single Cell kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The DNA was extracted from the FACS sorted cells and whole genomes were amplified using multiple displacement amplification technique (MDA) with the REPLI-g Single Cell kit (Qiagen) by following the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: Bulk-RNA free of genomic DNA for RT-qPCR analysis was extracted from cell pellets (≤ 2 x 106 cells) using the RNeasy plus spin-column purification kit (Qiagen) and performed using the Qiacube liquid handler (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... of either the B cell clone or polyclonal B cells of the patients were directly FACS sorted into 100µL ALT lysis buffer (Qiagen MicroKit). gDNA was extracted as per manufacturer’s instructions (Qiagen MicroKit) ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was extracted from cell pellets containing at least 1e6 cells/pellet using the DNeasy Blood and tissue kit (Qiagen), followed by bisulfite conversion using the Epitech Fast Bisulfite Conversion Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... AllStars Hs Cell Death siRNA (Cat# 1027298, Qiagen) was used as a positive control for transfection efficiency ...
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bioRxiv - Genetics 2021Quote: ... S2R+ cells were transfected using Effectene (Qiagen, 301427) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Sorted cells were collected in RLT buffer (Qiagen) and frozen at -80°C.
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: ... (AllStars Hs Cell Death Control siRNA from Qiagen), and siRNA against IKKγ ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed using RNeasy lysis buffer (QIAGEN) supplemented with DTT and proteins were degraded using proteinase K (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were either harvested for DNA extraction (Qiagen) or frozen down for long-term storage ...
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bioRxiv - Immunology 2020Quote: ... cells were suspended in RLT buffer (Qiagen®) before freezing at -80°C until analysis ...
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bioRxiv - Immunology 2022Quote: ... cells were lysed (RLT buffer, Qiagen, Valencia, California) and RNA was extracted (RNeasy Plus mini kit ...
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bioRxiv - Developmental Biology 2020Quote: ... Sorted cells were collected in RLT buffer (Qiagen) for RNA isolation.
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bioRxiv - Cancer Biology 2019Quote: A375 cells were stability transfected via Effectene (Qiagen) coupled with a piggyBac transposase integration system (43 ...
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bioRxiv - Developmental Biology 2019Quote: ... sorted cells were homogenized with a QIAshredder (QIAGEN)and total RNA extracted using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Systems Biology 2019Quote: ... cells were lysed using Buffer RLT Plus (Qiagen) with 1% beta-marcaptoethanol (Sigma) ...
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bioRxiv - Pathology 2019Quote: ... Fresh cells were processed through Qiashredder (Qiagen 79654) and RNeasy mini kit (Qiagen 74104 ...