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Citations for Qiagen :
1 - 50 of 5289 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... we extracted total RNA from CD4+/CD8+/CD3+ T cells (from spleen or bone marrow) of Foxp3-GFP or C57BL/6 mice using RNeasy Micro Kit (Qiagen) and cleaned from excess DNA with DNAse 1 enzyme (Promega) ...
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bioRxiv - Immunology 2020Quote: RNA was isolated from CD3+ T-cells using the Qiagen RNeasy Plus Mini Kit or EN AllPrep Mini Kit with on column DNaseI (Qiagen) digestion ...
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bioRxiv - Immunology 2020Quote: ... was isolated from previously sorted T cells and monocytes (CD19-CD3+/CD14+)30 using the QiaAmp DNA mini kit (Qiagen), and the concentration was measured on Nanodrop.
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bioRxiv - Cell Biology 2019Quote: ... conjugated with anti-penta-his biotin antibody (Qiagen) for 1 and half hours at 4°C ...
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bioRxiv - Immunology 2021Quote: ... CD3+CD8+CD45RA- and CD3+CD4+CD45RA-cell populations were bulk sorted then frozen into RLT buffer (Qiagen).
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bioRxiv - Biophysics 2020Quote: ... the surface was incubated with 1 nM biotinylated penta-His antibody (Qiagen) in buffer A for 10 min ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from cultured in vitro or in vivo primed T cells or ex vivo sorted T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... plasmids bearing chimeric antibody heavy and light chain genes were prepared (EndoFree Plasmid Mega prep Kit, QIAGEN) and transfected into Human Embryonic Kidney cells (HEK293F ...
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bioRxiv - Biophysics 2021Quote: ... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
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bioRxiv - Cancer Biology 2019Quote: ... Pathways significantly affected in non-T cell-inflamed or non-T cell-inflamed-enriched mutated genes were identified by IPA® (QIAGEN Inc., Germany).
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bioRxiv - Cancer Biology 2019Quote: ... T cells were sorted directly into 250μl RNAprotect buffer (Qiagen), spun down for 1 minute at 2000 RPM ...
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bioRxiv - Biophysics 2023Quote: ... the glass was additionally treated with biotinylated anti-hexahistidine monoclonal antibody (Penta-His Biotin Conjugate; Qiagen) as in Duchi et al.23 and Dulin et al.31.
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bioRxiv - Biophysics 2023Quote: ... 20 nM biotin-NTA (QIAGEN) was charged with 50 nM Ni2+ and incubated on the slide for 10 min before rinsing away the unbound excess biotin-NTA and Ni2+ with PBS (this step was omitted for the labeled DNA samples) ...
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bioRxiv - Immunology 2021Quote: Purified CD4+ T cells were homogenized using the Qiashredder (Qiagen, 79654) and RNA was extracted using the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... gliding motility assays were performed with specific immobilization of motors on the surface via penta-His antibodies (34660, Qiagen) as previously described[64] (Figure S4) ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was extracted from flow-sorted CD3+ cells using the RNEasyPlus Micro Kit (Qiagen, 74034), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Sorted T cells were lysed in Buffer RLT Plus (Qiagen, Hilden, Germany) with 1% β-mercaptoethanol (Millipore-Sigma ...
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bioRxiv - Immunology 2020Quote: ... or CD4+ T cells was extracted using the RNeasy micro kit (Qiagen) followed by cDNA synthesis using the iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from isolated CD8+ T cells (RNeasy kit, Qiagen) and 300 ng of RNA were used for cDNA synthesis (First-Strand Synthesis kit ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from murine T cells via RNeasy Kit (Qiagen) with DNase digestion (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... and CD95+ CD4 T cells using a RNeasy plus mini kit (QIAgen). Isolated RNA sample quality was assessed using a BioAnalyzer RNA pico assay (Agilent Technologies Inc. ...
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bioRxiv - Immunology 2024Quote: ... total mRNA was isolated from mouse T cells by RNeasy Micro kit (Qiagen), reverse transcribed from mRNA to cDNA for subsequent real-time PCR analysis ...
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bioRxiv - Immunology 2024Quote: ... total RNA was extracted from T cells by using RNeasy mini kit (QIAGEN) and reverse-transcribed into cDNA with Superiorscript III reverse transcriptase (Enzynomics ...
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bioRxiv - Immunology 2024Quote: T cells were subjected to RNA extraction utilizing the RNeasy Micro Kit (QIAGEN). RNA from the renal cortex was isolated with the NucleoSpin Kit (Macharey-Nagel ...
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bioRxiv - Microbiology 2020Quote: ... and a plasmid expressing VSV-G or HIVKB9 envelope glycoproteins were cotransfected at the mass ratio of 9:1 (9 Hi.fate / 1 Env) using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: Relative telomere length of MDS patients was measured by quantitative polymerase chain reaction (qPCR) as previously reported.8 K562 cell DNA was extracted using the QIAamp Blood Mini kit (Qiagen). Telomere length was measured by two orthogonal methods ...
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bioRxiv - Cell Biology 2022Quote: ... Drosophila S2 cells were transfected with mNeonGreen-tagged kinesin-1 full-length heavy chain (KHC) and Halo-tagged PLP584-1811 (Effectene, QIAGEN). The lysates were harvested for TIRF motility assays at ∼48 h post-transfection ...
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bioRxiv - Immunology 2022Quote: ... Enriched cells were surface stained and 5-20 ×103 intratumoral cDC1s were sorted directly in RLT lysis buffer (Qiagen) containing 2-mercaptoethanol ...
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bioRxiv - Biophysics 2021Quote: ... NF-κB molecules were immobilized through the 6xHis-tag on the C-terminus of RelA by penta-His antibody biotin conjugate (34440, Qiagen). For DNA-bound NF-κB experiments ...
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bioRxiv - Clinical Trials 2019Quote: Genomic DNA from 1-10 Million CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen). In some experiments ...
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bioRxiv - Microbiology 2020Quote: ... the variable region genes from each single cell were amplified in a reverse transcriptase polymerase chain reaction (RT-PCR: QIAGEN, Germany) using a cocktail of sense primers specific for the leader region and antisense primers to the Cγ constant region for heavy chain and Cκ and Cγ for light chain ...
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bioRxiv - Immunology 2021Quote: ... total RNA of CD8+ human T cells was extracted by miRNeasy Mini Kit (Qiagen), following the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2022Quote: Cultured human T cells after treatment were flash frozen in Buffer RLT (Qiagen, 79216) and kept in −80°C until processing ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA from human primary T cells was isolated using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from sorted CD4+ T cells using the RNeasy Mini kit (Qiagen). A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Polymerase chain reaction (PCR) was performed with the HotStarTaq Plus polymerase (Qiagen) with 5 min initial denaturation at 94°C ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA from 1-5 million total CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen) or phenol-chloroform and the DNA concentration was measured by Qubit High Sensitivity Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA (gDNA) was extracted from J-Lat cells 10.6 and HIV-1 negative CD4+ T cells with the QIAcube (Qiagen), using the QiaAmp DNA mini kit (Qiagen) ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were washed with PBS-T (PBS1x/0.1% Tween 20) and incubated with mouse antibody against 6xHis-tag (1:2,000; Qiagen), rabbit antibody against GST-tag (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... epithelial cells (DAPI-EGFP+CD45-) and fibroblasts (DAPI-EGFP-CD45-CD11b-CD31-CD3-) were sorted and lysed in RLT buffer (Qiagen). Total RNA was prepared using RNeasy (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from CD4+ T cells using the DNeasy blood and tissue kit (Qiagen), according to manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... The total RNA of sorted T cells was extracted with an RNeasy Micro kit (Qiagen) and reverse transcribed using reverse transcribed using SuperScript III Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... primary T cells and BJ-5at fibroblasts using the RNeasy Plus Mini Kit (Qiagen. #74136), reverse transcribed to first-stand cDNAs using iScript™ cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was extracted from sorted T cell populations with mini RNA-easy Kit (Qiagen). Equal amounts of total RNA from different groups were used for the assay ...
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bioRxiv - Immunology 2022Quote: ... DNA for library preparation was extracted from CD8+ T-cells using FlexiGene DNA Kit (Qiagen). 150 ng aliquots of obtained DNA were used as input to prepare each out of four TRA libraries ...
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bioRxiv - Immunology 2022Quote: ... CD4+/CD8+ T cells and Tfh from indicated organs using RNeasy Mini Kit (Qiagen Inc.). cDNA synthesis was performed using Superscript III First Strand Synthesis System (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... isolate DNA of CAR-T cell pellets with DNEasy Blood & Tissue Kit (Qiagen catalogue # 69506), perform PCR amplification with appropriate forward and reverse primers to TSC2 S1364A Δ insertion and then submit PRC products for sequencing (Genewiz ...
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bioRxiv - Immunology 2023Quote: T-cells or homogenized colon were suspended in RLT buffer (RNAeasy kit, Qiagen, Valencia, CA), RNA was extracted (RNAeasy ...