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Citations for Qiagen :
351 - 400 of 803 citations for St Louis Encephalitis virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Thawed RLT lysates were then run over a QIAshredder column (Qiagen, 79654) and subsequently over a gDNA eliminator column ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysate was subjected to genotyping using a HotStar Taq polymerase (QIAGEN) and previously established primers101 ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was incubated with equilibrated Ni-NTA Agarose resins (Qiagen #30210) at 4ℒC for 2-4 hours ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: ... RNA was isolated from the lysate using an RNEasy plus kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was incubated for two hours with Ni-NTA beads (Qiagen). Non-specifically bound proteins were removed by washing the beads with 20 mM NaPO4 pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequently RNA was extracted from tissue lysates using the RNeasy MiniKit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was purified from lysates using a RNeasy Plus Mini Kit (Qiagen). RNA-seq libraries and sequencing (RNA-seq ...
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bioRxiv - Microbiology 2024Quote: ... The cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen) on a rotator for 1 hour at 4°C ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted from tissue lysate by RNAEasy Mini column purification (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen) and linear acrylamide (ThermoFisher Scientific ...
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bioRxiv - Epidemiology 2019Quote: Total RNA was extracted from SFTS virus IgM positive samples using a QIAamp viral RNA mini Kit (Qiagen, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Viral RNA (vRNA) was isolated from virus-infected lung homogenate supernatant using the QIAamp Viral RNA Mini Kit (QIAGEN). Influenza genome copies were quantified from RNA isolations by reverse transcription and quantitative PCR (RT-qPCR ...
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bioRxiv - Microbiology 2021Quote: Virus isolation was performed on tissues following homogenization in 1 mL DMEM using a TissueLyser (Qiagen, Germantown, MD, USA) and inoculating Vero E6 cells in a 96 well plate with 200 µL of 1:10 serial dilutions of the homogenate ...
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bioRxiv - Biophysics 2021Quote: The SARS-CoV-2 RNA was isolated from virus particles with the QIAmp viral RNA mini kit (Qiagen - USA) and reversely transcribed to cDNA with the High-Capacity Reverse Transcription Kit (Thermo - USA) ...
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bioRxiv - Microbiology 2020Quote: ... followed by extraction of viral nucleic acids using a commercial kit (QIAamp MinElute virus spin kit, Qiagen, Venlo, Netherlands). A portion (2.5 µl ...
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bioRxiv - Microbiology 2021Quote: ... the resulting filtrate was used for viral DNA extraction using the QiAmp Ultra-Sensitive Virus Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Nucleic acid was first extracted from each blood sample using QIAamp MinElute Virus Spin kits (Qiagen, Mississauga, Ontario, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Purified ZIKV RNA and HSV-2 DNA were extracted using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704), and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and subsequently extracted using the Qiagen EZ1 Advance XL extractor with the EZ1 Virus Mini Kit v2.0 (Qiagen, Germany). For the eastern Australian RSV cases ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 10,000 TCID50 WT and NS1R38A virus containing inoculum using the QIAamp Viral RNA mini kit (Qiagen), according to the manufactures protocol ...
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bioRxiv - Immunology 2021Quote: ... viral RNA was extracted from AFs stocks using the QIAamp MinElute Virus Spin kit (cat. n° 57704, Qiagen, Germany), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Fold changes of N transcript expression with each treatment were calculated corcenning to the Virus control using REST 2009 program (v2.0.13, Qiagen, GbmH) [26] ...
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bioRxiv - Microbiology 2022Quote: ... Sample DNA was extracted from the viral enriched filtrate using the QiAmp Ultra-Sensitive Virus Kit (Qiagen, Hilden, Germany) and was subjected to whole genome amplification (WGA ...
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bioRxiv - Microbiology 2022Quote: ... followed by total nucleic acid extraction of 400 ul of pretreated stool using the EZ1 Virus Mini Kit v2.0 (Qiagen). Extracts were eluted in 60 ul volume.
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bioRxiv - Immunology 2022Quote: Serum viral RNA was extracted from 200 μl using the QiaAmp Min Elute Virus Spin kit (Qiagen, Cat# 57704) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Viral genomic RNA was purified from 280 μL virus-containing media using the QIAamp Viral RNA Mini Kit (Qiagen) as per the manufacturer’s instructions and eluted in 60 μL nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: We extracted influenza virus genomic RNA from the clarified allantoic fluid using the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen) and linear acrylamide (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... RNA from one aliquot per condition per virus isolate and negative control was immediately extracted with the QIAamp Viral RNA Mini Kit (Qiagen) and stored at −80°C until further processing ...
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bioRxiv - Microbiology 2021Quote: Total RNA of each isolate was extracted from frozen virus stock using QIAamp Viral RNA Mini Kit (Qiagen, Germantown, MD) following the manufacturers spin protocol ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the working stock of each reporter virus and treated with DNase using the DNase Max kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: 200µL of lung clarified homogenate or infectious cell supernatant (virus stock) was inactivated with an equal volume of VXL lysis buffer (Qiagen) and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted from 70 μL of supernatant using QIAamp 96 virus QIAcube HT kit on the QIAcube HT System (Qiagen). Each RNA sample was analyzed by one-step RT-qPCR with two SYBR Green assays ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from supernatants of swab material using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Total Nucleic Acid (TNA) was extracted from using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (Qiagen). Autopsy tissues were collected from lung ...
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bioRxiv - Microbiology 2019Quote: For viral genome copy measurements RNA was extracted from 2 µl sucrose purified virus using the RNeasy mini kit (QIAgen). The RNA was then treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from supernatants of hamster nasal wash samples using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). Real time RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: Viral RNA extraction was performed on an EZ1 Advanced XL device using the EZ1 virus mini kit V2.0 according to the manufacturer’s recommendations (Qiagen, Courtaboeuf, France). The qPCR was run on a Lightcycler® 480 thermocycler (Roche diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from cell culture supernatants or hamster nasal washes using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: Total viral RNA was extracted from deactivated samples using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (QIAGEN). One step reverse transcription to cDNA and real-time PCR (RT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... MB897-1) were used to generate infectious virus by transfecting 293T cells using the Effectene transfection reagent (QIAGEN, Valencia, CA). Supernatant containing virus was collected 2 days post transfection ...
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bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from either DF-1 cultures infected with the WT virus or DF-1P cells using the Qiamp viral RNA mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... EAV genome copy number was determined by one step RT-qPCR using the QuantiTect™ Virus + ROX Vial Kit (Qiagen). Each RNA sample was tested in triplicate ...
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bioRxiv - Physiology 2021Quote: ... We infected Sf9 cells with the recombinant virus to express and purify the His-tagged proteins using a nickel-nitrilotriacetic acid (Ni-NTA, Qiagen) column ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA from the original material used for next-generation sequencing of the serum and passage 3 cultured MRI103 virus was used as the template for amplification using the One-Step Ahead RT-PCR kit (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... virus pellets were resuspended by vortexing and community DNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed with a primer set targeting partial regions of the ORF1b gene in the SARS-CoV-2 virus using the QIAGEN OneStep RT-PCR kit (Qiagen) as previously reported (51) ...
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bioRxiv - Genomics 2021Quote: ... Total Nucleic Acid (TNA) was extracted using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (Qiagen). RNA isolation and library preparation is fully described in Butler ...
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bioRxiv - Immunology 2022Quote: ... we isolated viral RNA directly from the Fluenz Tetra vaccine (AstraZeneca; season 2017/2018) virus using the Qiagen Viral RNA Isolation Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were cultured in growth media containing virus for 60 hours and total RNA was then extracted with the RNeasy kit (Qiagen). From the total RNA ...