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Citations for Qiagen :
101 - 150 of 6945 citations for Somatostatin Receptor 4 SSTR4 Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... coli and purified by His-tag affinity purification using Ni-NTA agarose beads (Qiagen) followed by ion exchange purification using a mono-Q HR 5/5 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The target proteins were purified by affinity chromatography using a Ni-NTA column (Qiagen) with the lysis buffer supplemented with 20 mM and 200 mM imidazole serving as the washing buffer and elution buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was purified by affinity chromatography using Ni-NTA agarose (Qiagen, Hilden, Germany), followed by gel filtration chromatography ...
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bioRxiv - Cell Biology 2020Quote: ... were purified by two rounds of nickel affinity chromatography using Ni-NTA agarose (QIAGEN), with overnight cleavage by N-terminally His-tagged TEV protease (expressed in our laboratory ...
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Large-scale conformational changes of FhaC provide insights into the two-partner secretion mechanismbioRxiv - Biophysics 2022Quote: ... Fha44 was affinity-purified from the supernatants with Ni-NTA beads (Qiagen, Courtaboeuf, France). The membrane extracts were prepared and FhaC was detected as above ...
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bioRxiv - Biophysics 2019Quote: ... Proteins were purified by immobilized metal affinity chromatography according to the manufacturer’s protocol (Qiagen), followed by gel filtration in 10 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... and purified using immobilized metal affinity chromatography using Ni-NTA resin (Qiagen, Hilden, Germany). Eluted proteins were renatured by three-step dialysis to a final buffer composition of 20 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified by affinity chromatography using a Ni-NTA agarose resin (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
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bioRxiv - Microbiology 2023Quote: ... and affinity-purified in a gravity flow column using Ni2+-NTA-agarose beads (Qiagen). After binding of the His-tagged proteins to the columns ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged proteins were purified by affinity chromatography on Ni-NTA Agarose resin (Qiagen) and eluted with buffer containing 0.5 M imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The labeled cDNAs were purified with a QIAquick PCR purification kit (Qiagen). DNA microarrays were processed and analyzed as previously described [59] ...
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bioRxiv - Genomics 2020Quote: ... Labeled PCR product was purified with a QIAquick PCR Purification Kit (QIAGEN) and 50ng was mixed with hybridization buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Labeled DNA was purified using a spin column PCR purification kit (Qiagen) and eluted with 10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The monomeric ACE2 was purified by affinity chromatography using Ni-NTA agarose (Qiagen, Hilden, Germany), followed by gel filtration chromatography ...
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bioRxiv - Cell Biology 2020Quote: ... induced in BL21-CodonPlus(DE3)-RIL Escherichia coli and affinity purified with Ni2+ agarose (Qiagen). The purified His6Tam41 protein was used as an antigen in rabbits (Pacific Immunology) ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were purified under native conditions by affinity chromatography using Ni–NTA agarose (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified from clarified lysate by affinity chromatography on Ni2+-nitrilotriaceta-agarose (Qiagen) in 20 mM Tris-HCl 500 mM NaCl pH 8.0 buffer and by size exclusion chromatography on Superdex 200 HL 16/600 column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... BapR-CPD-His was affinity purified from cleared lysates using Ni-NTA agarose beads (Qiagen) with gentle rocking at 4°C for 2 hours ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant Lsr2 proteins were purified using immobilized-metal affinity chromatography (Ni-NTA agarose, Qiagen) and size exclusion chromatography ...
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bioRxiv - Molecular Biology 2019Quote: ... His6- and GST-tagged proteins were affinity-purified in batch on Ni-NTA agarose (Qiagen) and glutathione Sepharose 4 Fast Flow (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tagged AC was secreted into the medium and purified by nickel affinity chromatography (Qiagen) with elution in 100 mM citrate pH 4.0.
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bioRxiv - Biochemistry 2022Quote: ... Sso7dmut-sortase cleavage site-IN was purified with nickel affinity chromatography (Ni-NTA Superflow, Qiagen). The hexahistidine tag and Sso7dmut were cleaved from HIV-1 IN by addition of his-tagged sortase and GGGC peptide (GenScript) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cleared lysate was purified by affinity chromatography on a Ni-NTA Superflow Cartridge (Qiagen) using an ÄKTA Start (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was purified by affinity chromatography on a Ni-NTA Superflow Cartridge (Qiagen) using an ÄKTA Start (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was purified by affinity chromatography on a Ni-NTA Superflow Cartridge (Qiagen) using an ÄKTA Start (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... the proteins were purified by Ni-NTA affinity chromatography using Ni-NTA agarose beads (Qiagen) and gel filtration with a HiLoad 16/600 Superdex 75 pg column in buffers containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Physiology 2024Quote: ... C3 constructs were purified from the soluble fractions by nickel-based affinity (Ni-NTA, Qiagen). The most concentrated fraction was further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Synthetic Biology 2023Quote: ... All proteins were purified by gravity flow Ni2+ -NTA affinity chromatography (Qiagen, Cat. -No. 30230), except TEV-construct which was purified by gravity flow Strep-Tactin®XT Superflow® high-capacity resin (IBA Cat ...
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bioRxiv - Bioengineering 2024Quote: ... and was then subjected to Ni-NTA affinity purification at 4 [(Qiagen, Valencia, CA). The column was adequately washed with 20 column volume of wash buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2020Quote: ... BipA-His was purified from the soluble fraction by affinity chromatography using Ni-NTA agarose (QIAGEN) previously equilibrated with equilibration buffer ...
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bioRxiv - Molecular Biology 2021Quote: Proteins were purified from Sf21 insect cells by affinity purification with Ni-NTA agarose (Qiagen, 30230) and ...
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bioRxiv - Molecular Biology 2021Quote: ... 6x-His-Smt3 was purified from BL21 E.coli cells using a Ni-NTA affinity column (Qiagen) following manufacturer indications ...
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bioRxiv - Microbiology 2021Quote: ... Toxins were then purified from bacterial cell lysates through affinity chromatography on a Nickel-resin (Qiagen), ion exchange ...
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bioRxiv - Plant Biology 2021Quote: ... HIS-tagged proteins were purified by metal-affinity chromatography using Ni-NTA resins (Qiagen, Hilden, Germany). Protein concentration was estimated by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tagged protein was purified by affinity chromatography on Ni-NTA agarose beads (Qiagen 30230) and cleaved on beads with TEV protease o/n at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was purified over Ni-NTA affinity resin according to the manufacturer’s recommendation (Qiagen). Eluted proteins were pooled and dialyzed overnight at 4°C into 50 mM Na2HPO4 pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... the resolubilized histidine-tagged histones were purified with Ni affinity chromatography using Ni-NTA beads (Qiagen). For untagged H2B ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was then purified on Ni-NTA affinity chromatography with Ni-NTA agarose resin (Qiagen) following the protocol described in Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... coli under a T7 promoter and purified using Ni-NTA nickel affinity beads (Qiagen, Hilden Germany). Purified protein was dissolved in PBS solution and lyophilized ...
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bioRxiv - Immunology 2023Quote: ... Proteins and complexes were purified from the cell supernatants using Nickel-NTA affinity resin (Qiagen: 30210), followed by Superose 6 (Cytiva ...
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bioRxiv - Molecular Biology 2024Quote: ... The fusion protein in the lysate was affinity-purified by Ni-NTA agarose beads (Qiagen, 30210) capturing HIS tag or by glutathione sepharose 4B beads (Amersham ...
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bioRxiv - Systems Biology 2021Quote: ... Purified labeled RNA (LRNA) and FRNA were subjected to DNase digestion (Qiagen, 79254). Total fragmented RNA and labeled RNA libraries were prepared with Ovation Universal RNA-Seq kit (NuGEN ...
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bioRxiv - Genetics 2023Quote: ... The labeled DNA was purified using the Qiaquick PCR purification kit from Qiagen, eluted in 30 μl of water ...
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bioRxiv - Genomics 2023Quote: ... The labeled DNA was purified using the Qiaquick PCR purification kit from Qiagen, eluted in 30 µl of water ...
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bioRxiv - Microbiology 2023Quote: ... Then this amplified product was labeled with biotin at 5’ end followed by purification using the Qiaquick nucleotide removal kit (QIAGEN).To check the drug-protein interaction ...
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bioRxiv - Cell Biology 2020Quote: ... HsKinesin-II was purified using Ni column and MBP column successively (Ni-NTA agarose affinity resin, QIAGEN; Amylose resin ...
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bioRxiv - Biochemistry 2019Quote: ... 20mM Imidazole) and Noxa was purified by affinity chromatography on a Nickel-NTA column (Qiagen, Valencia, CA). Noxa was eluted with a buffer containing 10mM HEPES (7.2) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The RBD was purified from the expression media by metal-affinity chromatography using Ni-NTA beads (Qiagen). The Fabs were purified from the expression media using rProtein A Sepharose Fast Flow beads (GE healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... for 45 min and the supernatant was purified using affinity chromatography with a Ni-NTA column (Qiagen). Ni-NTA column bound with MBP-GLIC fusion protein was washed with 5 column volumes of Buffer B1 with 50 mM imidazole and 1% DDM ...