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Citations for Qiagen :
551 - 600 of 6945 citations for Somatostatin Receptor 4 SSTR4 Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Cell debris and protein aggregates were removed by centrifugation and the supernatant was loaded on a Ni-NTA Superflow affinity resin (Qiagen). The resin was washed with lysis buffer and the protein eluted with lysis buffer containing 200 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... All the constructs also contain a His6-tag at the N-terminus for affinity purification with Nickel-NTA agarose beads (Qiagen). Site-directed mutagenesis was performed using the QuickChange Lightning kit (Agilent Technology) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell extract was incubated for 10 min at +4°C and clarified by centrifugation at 39 000 g for 30 min and immediately processed to metal (Ni2+) affinity purification using Ni2+-NTA agarose beads (Qiagen) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Metal-chelate affinity chromatography was used to purify the 6xHis-tagged proteins using the Ni-NTA Fast Start Kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... the supernatants were mixed with Sigma HIS-Select Nickel Affinity Gel (at a ratio of 3:1) equilibrated in lysis buffer before being added into a polypropylene column (Qiagen). After extensive washing with a cold lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: c-miR isolations from blood serum were carried out using affinity column-based miRNeasy Serum/Plasma Advanced Kit (217204, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified using Ni-NTA immobilized metal-affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow Resin (GE Lifesciences) or NiNTA Agarose (QIAGEN) and gravity flow ...
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bioRxiv - Genetics 2020Quote: ... The supernatant of the transfected cells encoding for N-HIS-FAM19A5 was collected for Ni-NTA affinity chromatography (Qiagen, Germany). Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Cell debris was cleared by centrifugation at 40,000 g for 45 min at 4°C and supernatant was applied to His-tag affinity purification using Ni-NTA agarose (beads or 5 ml column, Qiagen) in buffer A (50 mM TRIS ...
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bioRxiv - Immunology 2023Quote: ... we incubated both proteins for 1 day at 4°C in 1x PBS and purified the resulting mSav-cc-PS-CFP2 together with an excess of PS-CFP2-tetrazine via Ni-NTA based affinity chromatography (QIAGEN) and gel filtration (Superdex 200 10/300 GL ...
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bioRxiv - Microbiology 2023Quote: ... The cleared lysate was applied to an affinity chromatog-raphy column containing Ni-nitrilotriacetic acid (NTA) su-perflow resin (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Systems Biology 2022Quote: ... The purified PCR product was digested with HindIII and BamHI and purified by the PCR purification kit (Qiagen). The purified tapA promoter and gfp-lcn DNA fragments were mixed and ligated with pDG1664 digested with EcoRI and BamHI ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were weighed and homogenized in 4 volumes of water (4 µL of water/mg tissue, 4°C) using a bead beater (TissueLyser II, QIAGEN; Germantown, MD). Aqueous homogenates were profiled using four complimentary liquid chromatography tandem mass spectrometry (LC-MS ...
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bioRxiv - Microbiology 2020Quote: ... and purified using the RNAeasy Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and purified with RNeasy Mini kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... purified with MinElute PCR Purification Kit (Qiagen) and circularized by an intramolecular ligation reaction using the Quick Ligation™ Kit (New England Biolabs)
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bioRxiv - Microbiology 2019Quote: ... and purified by QIAquick Gel Extraction (QIAGEN). Single strand viral RNA was synthesized from the purified viral DNA substrate using ScriptMAX® Thermo T7 Transcription Kit (TOYOBO ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were purified using Qiaquick (Qiagen) columns ...
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bioRxiv - Immunology 2022Quote: ... purified using QIAquick Nucleotide Removal kit (QIAGEN) and analyzed on 10% Novex TBE Gels (Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... and purified using the RNeasy kit (Qiagen). Moreover ...
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bioRxiv - Neuroscience 2021Quote: ... and purified with Plasmid Plus Kit (QIAGEN). The lentiviruses were packaged in Lenti-X 293T cell line (Takara Bio ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was purified over RNAeasy columns (Qiagen). 1μg total RNA was used in a reverse-transcription (RT ...
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bioRxiv - Molecular Biology 2020Quote: ... purified with the PCR cleanup kit (Qiagen), ligated in 250μL with 3μL of T4 ligase (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... column-purified (Qiagen, QIAquick PCR purification kit), mixed in equimolar amounts ...
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bioRxiv - Cell Biology 2022Quote: ... The genomic DNA was column purified (Qiagen) and eluted in 35 μL nuclease free water.
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bioRxiv - Immunology 2022Quote: ... RNA was purified with RNAeasy kit (Qiagen) as described previously(Vijay et al. ...
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bioRxiv - Genomics 2020Quote: ... Transposed DNA was purified (MiniElute Kit, Qiagen) and PCR amplified for 5 cycles ...
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bioRxiv - Genomics 2020Quote: ... and the samples were purified by Qiagen MinElute PCR Purification kit (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... DNA was purified on MinElute columns (Qiagen). Libraries were amplified using the following PCR set up ...
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bioRxiv - Genomics 2019Quote: ... and purified using a Minelute column (Qiagen). Sonicated DNA was size selected on an 1,5 % agarose cassette aiming for an insert size of 300 bp to 500 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified using RNeasy Mini Kit (QIAGEN). A Ribo-Zero Gold rRNA Removal Kit (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... Purified PCR products (PCR purification kit, Qiagen) of CHPV and JEV positive samples were sent for sequencing ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... coli and purified with NiNTA matrix (Qiagen). Immunization of the rabbits was performed by Absea (Beijing ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was purified using RNAeasy kit (Qiagen) as per manufacturers specifications used for microarray analysis ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR fragments were purified from gels (Qiagen), their concentration measured using a BioPhotometer D30 (Eppendorf ...
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bioRxiv - Genetics 2019Quote: ... RNA was purified using either RNeasy (Qiagen) or Direct-zol (Zymo ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA was purified (Qiagen RNeasy Mini Kit) and ethanol precipitated ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were then column purified (Qiagen) and eluted in TE (10mM Tris-HCl pH 8.0 ...
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bioRxiv - Genetics 2019Quote: ... and purified with QIAEX II kit (Qiagen). PCR products from the first step were used as templates in the second PCR step ...
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bioRxiv - Immunology 2019Quote: ... PCR products were purified (Qiagen, Valencia, CA) and sequenced by Genewiz ...
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bioRxiv - Genomics 2019Quote: ... and purified using RNeasy mini kit (Qiagen). Libraries were constructed using NEBNext Ultra II RNA Library Prep kit for Illumina (New England Biolabs) ...
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bioRxiv - Cancer Biology 2019Quote: ... purified using QIAquick Gel Extraction Kit (Qiagen) and dephosphorylated using Antarctic phosphatase (New England Biolabs) ...
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bioRxiv - Genetics 2020Quote: ... DNA was purified with MinElute Kit (Qiagen). Quantification of ChIP and INPUT DNA was accomplished using Qubit 2.0 Fluorometer system (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... gel purified (Gel Extraction kit, Qiagen, Netherlands), and in vitro transcripted (Sp6 mMESSAGE mMACHINE kit ...
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bioRxiv - Immunology 2021Quote: ... and purified by RNeasy Mini Kit (Qiagen). Library construction and sequencing were performed by iRepertoire (Huntsville ...
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bioRxiv - Immunology 2021Quote: ... and purified using PCR purification columns (Qiagen). PCR was performed using primers in the CLEC7A locus (see Table 3) ...
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bioRxiv - Molecular Biology 2020Quote: ... purified using a silica spin column (Qiagen), quantified using a NanoDrop photospectrometer (Thermo ...
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bioRxiv - Bioengineering 2020Quote: ... and purified using RNeasy mini kit (Qiagen). The yield of RNA was quantified using a NanoDrop spectrometer (NanoDrop Technologies ...
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bioRxiv - Biochemistry 2020Quote: The digestion mixture was purified by Qiagen PCR purification kit ...
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bioRxiv - Biochemistry 2020Quote: The ligation mixture was purified by Qiagen PCR purification kit and then concentrated by GeneVac ...