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Citations for Qiagen :
1 - 50 of 788 citations for Sodium Voltage Gated Channel Alpha Subunit 10 SCN10A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tags ...
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bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tag ...
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bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse penta-His antibody (cat. no. 34660, QIAGEN) against His tags ...
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bioRxiv - Neuroscience 2020Quote: ... along with the human NaV β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.X:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transiently co-transfected with NaV1.2 and the accessory β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.2:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
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bioRxiv - Physiology 2022Quote: ... Using the Neuronal ion channel plate (Qiagen, UK), 84 ion channels as well as housekeepers were measured in each sample ...
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bioRxiv - Immunology 2022Quote: 5000-25000 P3/P4 LP macrophages from bone marrow chimeras 8 weeks following reconstitution were FACS-sorted (gated on: CD103-, Ly6G-, CD11b+, CD11cint, CD64+, Ly6C-, CD45.1+ or CD45.2+) directly into RLT buffer (Qiagen) on a FACS Aria II (BD Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... WGA-AF488+ bright cells were gated compared to unstained TG control and sorted directly to RLT buffer (Qiagen) containing 1% 2-mercaptoethanol (Sigma ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from 10000 sorted cells (gated using strategies shown) from livers of C57BL/6 mice using a RNeasy Plus micro kit (QIAGEN). Sensifast cDNA synthesis kit (Bioline ...
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bioRxiv - Molecular Biology 2019Quote: ... resuspended in 50 mM sodium citrate buffer containing 10 µl of RNase A (20 mg/ml, Qiagen 76254) and incubated for 2 hours at 50°C ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Immunology 2022Quote: ... Antigen specific B cells were gated with CD19+/IgM-/IgD-/Ghost dye-/PE+/BV605+ and sorted in catch buffer B (Qiagen TCL Buffer + 1% beta mercaptoethanol) by one cell per well in a 96 well plate ...
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bioRxiv - Molecular Biology 2023Quote: ... the FT and Eluate fractions (90 µL each) were mixed with 10 µL 3 M sodium acetate and applied to a QIAquick spin column (Qiagen). Purified DNA was visualized a 1.3% agarose / 0.5x TBE gel and SYBR Green staining ...
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bioRxiv - Bioengineering 2021Quote: ... Intestinal epithelial cells in the apical channel were lysed and collected with RLT buffer (Qiagen) with 1% 2-Mercaptoethanol (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... A 10 nM solution of biotinylated penta-His antibody (Qiagen) was incubated for 10 min on the neutravidin-coated surface and excess unbound antibody removed (22 ...
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bioRxiv - Molecular Biology 2023Quote: ... 600 µl of lysis buffer (TES (0.1 M TRIS, 10 mM EDTA, 2% sodium dodecyl sulphate; pH 8) and Proteinase K (Qiagen, 20 mg/ml) in a 20:1 ratio ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative RT-PCR Array: Total RNA from microfluidic channels was purified using an RNeasy Mini Kit (Qiagen). Next ...
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bioRxiv - Microbiology 2021Quote: ... then sodium bisulfite-converted (EpiTect Bisulfite kit, Qiagen). The 5’LTR or the rev regions were amplified by (semi)nested PCR (primer sequences are available upon request) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and treated the pools with sodium bisulfite (EpiTect, Qiagen) following a protocol for formalin-fixed paraffin-embedded samples (Gu et al ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted from sorted cells (1,3×107 cells per channel) using the QIAamp DNA Mini kit (Qiagen). Gene-trap insertion site recovery and sequencing libraries were generated as previously described 60.
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bioRxiv - Biophysics 2020Quote: ... Cells were transiently transfected with cDNA encoding the mouse Piezo1 channel or its mutants tagged with GFP on its N-terminus in the pCDNA3 vector using the Effectene reagent (QIAGEN). Cells were then trypsinized and re-plated on poly-D-lysine-coated round coverslips 24 hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes containing the channels were collected by centrifugation (100,000×g) and proteins were extracted with 40 mM DM.65 Channels were purified using Ni-NTA agarose beads (Qiagen). Liposome reconstitution was performed using E ...
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bioRxiv - Bioengineering 2023Quote: RNA was extracted from the cells after dynamic culture by removing the medium from the channels and adding the RLT buffer (RNeasy Kit, Qiagen) for cell lysis and consequent RNA isolation ...
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bioRxiv - Immunology 2021Quote: ... a new empty 200 µL filtered tip was inserted to top channel outlet and 100 ul RNase easy lysis buffer (Qiagen, #74034) was used to lyse epithelial cells by quickly pressing and releasing the micropipette plunger 3 times ...
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bioRxiv - Plant Biology 2022Quote: ... and sodium bisulfite treated using the Epitect Bisulfite Conversion kit (QIAGEN). The region of interest within the MeSWEET10a promoter was amplified using the primers in Extended Data Table 3 ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... 0.005% sodium dodecyl sulfate) supplemented with 5LμL of Proteinase K (Qiagen). The cells were lysed by incubation at 55L°C for 1Lh ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... 0.005% sodium dodecyl sulfate) supplemented with 5LμL of Proteinase K (Qiagen) after the growth medium was removed ...
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bioRxiv - Molecular Biology 2019Quote: ... The cloned plasmid was amplified in DH5-Alpha cells and purified with QIAfilter™ Plasmid Maxi Kit (Qiagen, catalogue number 12243). Plasmid concentration was measured using Qubit (Thermofisher ...
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bioRxiv - Synthetic Biology 2023Quote: ... Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with LZRS-vector constructs using Effectene transfection reagent (Qiagen, 301425) as follows ...
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bioRxiv - Cancer Biology 2019Quote: All tissues were dissociated in 100 mM sodium carbonate using TissueLyzer II (QIAGEN) for 0.5 min at a rate of 0.30 repetitions three times and stored on ice for 30 sec between the dissociation cycles ...
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bioRxiv - Physiology 2020Quote: ... with 0.1% (w/v) sodium dodecyl sulphate (SDS) with a Tissue Ruptor (Qiagen). Samples were centrifuged for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % (w/v) sodium deoxycholate) and once with 500 µl EB buffer (Qiagen). Beads were treated with 300 µg/ml RNase A ...
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bioRxiv - Cell Biology 2020Quote: RNA FISH was performed using a FITC-labeled DNA oligo probe complementary to alpha satellite (Integrated DNA Technologies, Newark, NJ) or a biotinylated locked nucleic acid (LNA) oligo probe complementary to HSATII (QIAGEN, Hilden, Germany) (see Key Resources Table) ...
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bioRxiv - Cell Biology 2021Quote: ... Sodium bisulfite conversion of unmethylated cytosines in DNA was based on EpiTect Bisulfite Handbook (Qiagen). We designed primers (Table S3 ...
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bioRxiv - Biochemistry 2022Quote: ... as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000, Qiagen) for one hour ...
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bioRxiv - Cell Biology 2021Quote: ... Two μg of DNA was sodium bisulphite modified using the Eptech Kit (Qiagen GmbH, Hilden, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Cell pellets were resuspended in 50 mM sodium citrate buffer and treated with RNase A (QIAGEN) for 2 hours at 50°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 0.1 mM sodium orthovanadate and 0.2 mM phenylmethylsulfonyl fluoride (PMSF)) with a Tissue RuptorTM (Qiagen, Netherlands) homogenizer ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μg of RNA was resuspended in 10 μl of RNAse free water and mixed with sodium bisulfite pH 5.0 (42.5 μl) and DNA protection buffer (17.5 μL) (EpiTect Bisulfite Kit, Qiagen). The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... Adapter-ligated DNA was treated with sodium bisulfite using the EpiTect Plus Bisulfite kit (QIAGEN, Hilden, Germany) and amplified using the Kapa HiFi Uracil + ReadyMix (Roche ...
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bioRxiv - Paleontology 2019Quote: ... DNA was then exposed to two rounds of sodium bisulfite treatment using the EpiTect Bisulfite kit (QIAGEN), and paired-end DNA sequencing was performed using the Illumina Hi-Seq 2000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The complex was initially crystallized in a buffer 0.1 M MES pH 6.5 and 0.6 M sodium fluoride from Anions Suite crystallization screen (Qiagen). After optimization of crystallization conditions ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Neuroscience 2021Quote: Five hundred ng of DNA was treated with sodium bisulfite using the EpiTect Fast DNA Bisulfite kit (Qiagen). Primers for nested PCR (2 fragments for HCRT ...
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bioRxiv - Microbiology 2021Quote: ... 1mM PMSF and 0.25% sodium deoxycholate and the clarified lysate was loaded on a Ni-NTA column (Qiagen). Non-specifically bound proteins were removed by washing with lysis buffer containing 20mM imidazole and the proteins eluted with buffer containing 150mM imidazole ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng of each native library were treated by sodium bisulfite using the EpiTect Bisulfite kit (Qiagen, Valencia, CA) and purified according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... De-crosslinked DNA was added with 10μl 3M sodium acetate pH 5.2 and purified with QIAquick PCR Purification kit (QIAGEN 28106) according to manufacture instruction ...
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bioRxiv - Plant Biology 2023Quote: ... Adaptor-ligated libraries were subjected to two successive treatments of sodium bisulfite conversion using the EpiTect Bisulfite kit (Qiagen) as outlined in the manufacturer’s instructions ...