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Citations for Qiagen :
151 - 200 of 463 citations for SARS Coronavirus Nucleoprotein N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 replication was quantified via RT-qPCR using the QuantiTect Multiplex RT-qPCR Kit (Qiagen) with a Rotor Gene Q cycler (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: RNA and DNA was harvested from the BHK-SARS-2R_GFP_NeoR_NL cell line using the RNeasy mini kit (Qiagen) and the QIAamp genomic DNA mini kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 S mutant viruses using RNeasy Mini kit (Qiagen), and the S gene was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... was purified from curd stomach milk collected from P7 offspring that were exposed to mCHD (n=4) or mHFD (n=4) using a combination of QIAzol and miRNeasy Mini Kit (217004; Qiagen, Toronto, ON, CA) as described previously (Izumi et al. ...
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bioRxiv - Bioengineering 2021Quote: DNA contents in native/control kidneys (n = 4) and decellularized kidneys (n = 4) were measured using a Qiagen DNeasy Kit (Qiagen, Valencia, CA, USA). The tissues were initially minced and stored overnight at −80°C ...
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bioRxiv - Physiology 2020Quote: Total RNA was extracted from the gill samples collected from both Lake Winnipeg walleye (n = 10) and Delta Marsh walleye (n = 21) using the RNeasy Plus Mini Kit (Qiagen, Toronto, ON, CA) following manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: DNA content in native/control kidneys (n = 4) and decellularized kidneys (n = 4) were measured using a Qiagen DNeasy Kit (Qiagen, Valencia, CA, USA). The tissues were initially minced and stored overnight at −80°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... coli BL21(DE3)pLysS and purified through Ni-NTA-agarose (Qiagen, #30210). Proteins were eluted by 200 mM imidazole ...
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bioRxiv - Cancer Biology 2019Quote: ... coli BL21(DE3)pLysS and purified through Ni-NTA-agarose (Qiagen, #30210) as previously described (21) ...
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bioRxiv - Biophysics 2020Quote: ... coli BL21 (DE3) (38) and purified using Ni-NTA affinity column (Qiagen), HiTrapTM Heparin HP column and HiTrapTM Q HP column (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... coli strain BL21(BE3) and purified using Ni-NTA agarose chromatography (Qiagen) as previously described (Xu et al. ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells and purified by FPLC using a Ni-NTA column (Qiagen) and Heparin (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... coli and purified by Nickel affinity chromatography (Ni2+-NTA-sepharose resin, Qiagen). His6-FUBI-eS30 WT and AA constructs were expressed in E ...
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bioRxiv - Bioengineering 2022Quote: ... coli and purified using a QiaPrep Spin Miniprep kit (Qiagen, Venlo, NL). All plasmids were subjected to Sanger sequencing to verify the presence of the correct sing guide RNA (sgRNA ...
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bioRxiv - Microbiology 2023Quote: ... coli was extracted with the MagAttract HMW DNA kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol for DNA extraction from Gram-negative bacteria ...
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bioRxiv - Genomics 2023Quote: ... coli RNA was isolated using an RNEasy column (Qiagen, Germantown, MD, USA) followed by polyadenylation with an E ...
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bioRxiv - Biochemistry 2023Quote: ... coli using CLC Genomic Workbench v9.01 (QIAGEN® CLC bio, Aarhus, Denmark). Furthermore ...
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bioRxiv - Cell Biology 2023Quote: ... coli bacteria (XL1Blue strain or DH5α strain) using plasmid extraction kits (QIAGEN; Venlo ...
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bioRxiv - Developmental Biology 2023Quote: ... coli was modified from the protein purification protocol of The QIAexpressionist (QIAGEN) as follows ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were prepared from VectorBuilder Escherichia coli stocks using Plasmid Miniprep (Qiagen) and ampicillin selection ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
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bioRxiv - Microbiology 2020Quote: ... for the SARS-CoV-2 low stringency screen with the QIAmp DNA Blood maxi kit (Qiagen ref. 51194). In a first PCR step ...
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bioRxiv - Immunology 2021Quote: RNA of cells exposed to SARS-CoV-2 was isolated with the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from 140 µl of virus stock (SARS-CoV-2 VOC Delta, GISAID ID: EPI_ISL_15250227) using a QIAamp Viral RNA Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: RNA was extracted from CaCo-2 cells infected with SARS-CoV-2 using the RNeasy Mini kit (Qiagen) including an in-column DnaseI treatment step ...
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bioRxiv - Genomics 2019Quote: ... tBLASTp analyses (e-value < 0.001) were performed using CLC Genomics Workbench (CLC bio Qiagen). Protein sequences were defined as orthologs if they were reciprocal-best BLASTp hits having an e-value < 10−10 ...
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bioRxiv - Microbiology 2022Quote: O/N cultures were mixed with RNA protect (QIAgen, UK) at a ratio of 1:2 and pelleted at 5000xg for 10 min ...
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bioRxiv - Plant Biology 2019Quote: ... coli BL21 from pET16b-based plasmids and affinity purification via Ni-NTA (Qiagen), followed by specificity tests (Supplemental Figure 14).
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bioRxiv - Microbiology 2020Quote: ... coli 10-beta cloning strain were purified using the standard QIAprep protocol (QIAGEN), then delivered to the Pseudomonas strain via previously published electroporation83 or conjugation84 protocols ...
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bioRxiv - Biochemistry 2020Quote: ... coli and purified using Qiagen Ni+2 agarose as per vendor protocol (Qiagen). Recombinant proteins were dialyzed in demethylation buffer overnight at 4°C prior to use in enzymatic assays.
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bioRxiv - Genomics 2022Quote: ... coli isolates from Germany was extracted using the QIAamp DNA Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... coli in LB Broth with 100ug/mL ampicillin using Qiaprep Spin columns (Qiagen).
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bioRxiv - Microbiology 2019Quote: ... coli host JM109 and isolated with a commercial plasmid isolation kit (Qiagen Inc.). For cloning PCR fragments ...
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bioRxiv - Developmental Biology 2020Quote: ... coli cells were isolated according to the QIAprep Spin Miniprep Kit protocol (Qiagen). A restriction reaction was then set up to linearize the plasmid DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... coli cells and positive colonies were collected using a Mini Prep kit (Qiagen) and sequenced using the U6 primer (Supplementary Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... coli strain TJ249 culture using the QIAGEN DNeasy Blood & Tissue kit (QIAGEN GmbH). The extracted genomic DNA was sequenced on the PacBio RS II platform using P6C4 chemistry at Macrogen (Tokyo ...
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bioRxiv - Biophysics 2023Quote: ... coli and purified using a combination of Ni-NTA affinity (Qiagen, Hilden, Germany) and size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... coli BL21 ClearColi cell expression system and purified by NiNTA-Agarose beads (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was isolated from Escherichia coli using a Spin MiniPrep kit (Qiagen) and transferred to X ...
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bioRxiv - Immunology 2021Quote: ... RNA of cells exposed to SARS-CoV-2 WT was isolated with the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: The SARS-CoV-2 RNA was isolated from virus particles with the QIAmp viral RNA mini kit (Qiagen - USA) and reversely transcribed to cDNA with the High-Capacity Reverse Transcription Kit (Thermo - USA) ...
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bioRxiv - Microbiology 2021Quote: The QIAseq SARS-CoV-2 Primer Panel paired with the QIAseq FX DNA Library construction kits (Qiagen GmbH, Germany) were used for enriching and sequencing the entire SARS-CoV-2 viral genome ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from supernatants of SARS-CoV-2-infected Vero E6 cells using the RNeasy kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: Libraries were constructed using QIASEQ SARS-CoV-2 Primer Panel and QIAseq FX DNA Library UDI-A kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... PCR based COVID-19 viral genome enrichment was performed using QIAseq SARS-CoV-2 Primer Panel Kit (Qiagen, Germany). A total of 98 primer pairs specific to COVID-19 were used for enrichment where primers pairs were distributed in two pools (Pool-1 and Pool-2) ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: All breast cancer specimens (n = 21) were processed through AllPrep (Qiagen) protocol for the lysis and extraction of RNA and proteins (Fig 1D) ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli DH10B strains using a QIAprep plasmid miniprep kit (Qiagen, Redwood City, CA, USA) and digested with PvuII (FastDigest ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (DE3) and purified with dextrin sepharose high performance (Qiagen, Chatsworth, CA, USA) using an affinity column (Qiagen) ...
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bioRxiv - Genetics 2020Quote: ... coli cultures by maxi-prep using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965) and quantified plasmid DNA using Qubit dsDNA HS Assay Kit (Thermo Fisher #Q32854) ...
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bioRxiv - Biophysics 2021Quote: ... coli and purified by His-tag affinity purification using Ni-NTA agarose beads (Qiagen) followed by ion exchange purification using a mono-Q HR 5/5 column (GE Healthcare) ...