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Citations for Qiagen :
51 - 100 of 1157 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: The total RNA was extracted from uninfected and SARS-CoV-2 infected mice lung tissue using RNeasy mini kit (QIAGEN #74104). The quantity of RNA was determined using Qubit RNA assay kit with Qubit 4.0 and the quality of RNA was tested using agarose gel electrophoresis and High Sensitivity Tape station Kit (Agilent 2200 ...
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from the lungs of uninfected and SARS-CoV-2 infected hamsters with or without immune suppression using Trizol reagent and purified by RNeasy mini columns (Qiagen, CA, USA) as described previously 61 ...
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bioRxiv - Microbiology 2020Quote: We evaluated the impact of using Nanotrap particles to capture and concentrate SARS-CoV-2 on several nucleic acid extraction methods: the QIAamp® Viral RNA Mini Kit from QIAGEN (52906); the RNeasy® Mini Kit from QIAGEN (74106) ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from serially diluted and the 4 different media SARS-CoV-2 samples using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, Hilden, Germany). Briefly ...
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bioRxiv - Genomics 2020Quote: ... 80 µl of Viral Transport Media that had previously stored a nasopharyngeal swab from a patient infected with SARS-CoV-2 were used for RNA isolation using the QIAamp Viral RNA Mini spin kit (Qiagen, Cat No./ID: 52904) according to manufacturer specifications ...
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bioRxiv - Genomics 2021Quote: Amplicon libraries for viral genome sequencing were prepared using QIAseq FX DNA Library Kit and QIAseq SARS-CoV-2 Primer Panel (Qiagen, cat. no. 180475, cat. no. 333896) as instructed by the manufacturer’s manual ...
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bioRxiv - Biophysics 2021Quote: ... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 and SARS-CoV RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... NF-κB molecules were immobilized through the 6xHis-tag on the C-terminus of RelA by penta-His antibody biotin conjugate (34440, Qiagen). For DNA-bound NF-κB experiments ...
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bioRxiv - Neuroscience 2023Quote: ... synthetic RNA spike-ins (UniSp2, UniSp4, UniSp5 RNA Spike-in mix; Qiagen) were added and used as quality controls for RNA isolation ...
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bioRxiv - Neuroscience 2021Quote: ... A set of 52 RNA spike-ins (QIAseq miRNA Library QC Spike-Ins – Qiagen) that spanned a wide range of concentrations were added to the recovered RNAs according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... A set of 52 RNA spike-ins (QIAseq miRNA Library QC Spike-Ins – Qiagen) spanning a wide range of molar concentrations were added to the recovered RNAs ...
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bioRxiv - Cell Biology 2019Quote: ... S2 cells were transfected using Effectene (Qiagen) accord to the instructions provided by the manufacturer.
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bioRxiv - Molecular Biology 2019Quote: ... S2 cells were transfected using Effectene (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... S2 cells were transfected using Effectene (Qiagen), according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... transiently transfected S2 cells using Effectene (Qiagen) were cultured in Schneider’s medium at 22 °C for four days ...
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bioRxiv - Biophysics 2021Quote: ... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
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bioRxiv - Systems Biology 2023Quote: ... 1ul ExiSEQ NGS Spike-in (Qiagen) was added to the extracted RNA ...
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bioRxiv - Microbiology 2022Quote: ... Antibodies against His-tag and S-tag were purchased from Qiagen and GenScript respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Those included UniSpike 2 and 4 from the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN, Hilden, Germany), which were used to monitor RNA isolation efficacy ...
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bioRxiv - Cell Biology 2021Quote: Total mRNA was isolated from HEK293 (2×106) and Jurkat cells (4×106) parental and MCU-KO cell lines using the RNeasy Mini Kit (Qiagen). Isolated RNA was then analyzed using a NanoDrop spectrophotometer (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... hexa-histidine tag (Qiagen), GFP (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... S2 cells were transfected using Effectene Transfection Reagent (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... S2 cells were transfected using Effectene Transfection Reagent (QIAGEN), as previously described99 with plasmids for Actin5C-GAL4 (a gift from Yasushi Hiromi ...
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bioRxiv - Neuroscience 2023Quote: ... we added 1 µl of the spike-in mix provided in the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN) to the tube ...
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bioRxiv - Molecular Biology 2022Quote: ... Spike-in cel-miR-39-3p (MS00019789, Qiagen) was used as an internal control.
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bioRxiv - Genomics 2020Quote: ... SARS-CoV-2 nucleic acids were isolated from 300 µl viral transport media using the QIAamp Viral RNA Mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... anti-Strep-tag (34850, Qiagen), anti-HSP90 (4874 ...
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bioRxiv - Biochemistry 2019Quote: ... anti-Strep-tag (34850; Qiagen), anti-His-tag (11922416001 ...
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bioRxiv - Immunology 2024Quote: ... anti-Strep Tag (Qiagen 34850), anti-MLKL (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: ... anti-strep tag (Qiagen, 34850), anti-6*his tag (Thermo Fisher Scientific,11533923) ...
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bioRxiv - Biophysics 2023Quote: ... 20 nM biotin-NTA (QIAGEN) was charged with 50 nM Ni2+ and incubated on the slide for 10 min before rinsing away the unbound excess biotin-NTA and Ni2+ with PBS (this step was omitted for the labeled DNA samples) ...
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bioRxiv - Cell Biology 2022Quote: ... Syn-cel-miR-39 spike in synthetic RNA (Qiagen) was added to monitor extraction efficiency ...
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bioRxiv - Physiology 2022Quote: ... and 0.15 fmol of the spike-in UniSp6 (Qiagen) were added to the cDNA to control for the reproducibility of the cDNA synthesis.
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bioRxiv - Microbiology 2021Quote: ... pCDNA-SARC-CoV-2 Wuhan-Hu-1 P681H S plasmid was then extracted using the QIAprep Spin Miniprep Kit (Qiagen N.V.) and Sanger sequencing was used to confirm incorporation of the mutation.
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bioRxiv - Microbiology 2021Quote: ... MERS-CoV viral RNA was detected via the UpE MERS-CoV assay (27) using the Rotor-GeneTM probe kit (Qiagen). MERS-CoV dilutions with known genome copies were run in parallel to allow calculation of genome copies in samples.
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bioRxiv - Neuroscience 2021Quote: HEK293 cells and MEFs were transiently transfected with PolyFect (Qiagen) and Lipofectamine 3000 (Thermo Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were transfected into S2 cells using Effectene Transfection Reagent (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... DNA constructs were transiently transfected into S2 cells using Effectene (Qiagen). EVs for Western blot analysis (Fig ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 RNA from the apical washes of the ALI HBE culture was isolated using QIAamp Viral RNA Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... StrEP-Tag (mouse monoclonal, Qiagen, #34850). Peroxidase-conjugated secondary antibodies against rabbit IgG (#7074 ...
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bioRxiv - Cancer Biology 2021Quote: ... HIS-tag (Qiagen 34610 1:100) and HER3 (R&D Systems AF4518 1:400) ...
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bioRxiv - Biophysics 2023Quote: ... anti-Strep-tag antibody (Qiagen, 34850), or anti-HA-tag antibody (Cell Signaling ...