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Citations for Qiagen :
351 - 400 of 1543 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... the solubilized His-tagged histones were purified using Ni-NTA beads (Qiagen). For untagged H2B ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tagged proteins were purified with HisBind NiNTA-agarose (Qiagen) according to the recommendations of the supplier ...
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bioRxiv - Cell Biology 2019Quote: ... coli lysates using a nickel-nitrilotriacetic acid superflow resin (Qiagen) then combined and sent for anti-sera production (Covance) ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21-RIL and purified using Ni-NTA agarose (Qiagen) and Mono S affinity chromatography ...
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bioRxiv - Microbiology 2019Quote: ... coli cells and purified using the QiaQuick Miniprep kit (Qiagen) and quantified via Qubit Fluorometric Quantitation (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... coli DH5α cells and purified with Qiagen miniprep kit (Qiagen). The recovered plasmid stocks were verified through sequencing (Eton Bioscience).
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bioRxiv - Microbiology 2021Quote: ... coli pKAS32 cultures using a QIAprep Spin Miniprep Kit (Qiagen) and restriction digested with SacI and XbaI ...
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bioRxiv - Developmental Biology 2022Quote: ... Coli using the Plasmid Plus Mega Kit (Qiagen, Cat#12981). To assess library complexity ...
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bioRxiv - Plant Biology 2021Quote: ... coli BL21 RIL cells and purified on NiNTA beads (Qiagen), then concentrated and desalted using Vivaspin 500 columns (Sartorius).
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bioRxiv - Biochemistry 2019Quote: ... coli DH5α cells following QIAprep Spin Miniprep Kit protocol (QIAGEN) and used to transform the expression strains E ...
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bioRxiv - Molecular Biology 2020Quote: ... coli with the QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions and subsequently sequenced using the BigDye Terminator v1.1 Cycle Sequencing Kit and an ABI 3130 capillary sequencer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... coli cell lysate using Ni-nitriloacetic acid agarose resin (Qiagen) under denaturing conditions according to the manufacturer’s guidelines ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli using an endotoxin-free Maxi kit (Qiagen, ON, Canada) and resuspended in nuclease-free water ...
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bioRxiv - Microbiology 2021Quote: ... coli TOP10 using a QIAprep Spin Miniprep Kit (Qiagen, Germany) and was verified by sequencing (using 9 pairs of overlapping primers ...
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bioRxiv - Genomics 2021Quote: ... coli cultures using the DNeasy UltraClean Microbial Kit from Qiagen according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... coli DH5α cells following QIAprep Spin Miniprep Kit protocol (QIAGEN) and used to transform the expression strains E ...
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bioRxiv - Molecular Biology 2022Quote: ... coli (DH5α) and purified using the Plasmid Maxi Kit (Qiagen). Site-directed mutagenesis was used to introduce single nucleotide variants into the generated construct ...
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bioRxiv - Microbiology 2023Quote: ... coli HST08 strain using the QIAprep Spin Miniprep Kit (Qiagen). Recombinant and empty pVRL1Z plasmids were transformed into electro-competent A ...
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bioRxiv - Biochemistry 2022Quote: ... coli codon optimized L1 ORF0 sequence generated by Miniprep (Qiagen). 8nM EN WT and inhibitors or vehicle were incubated at room temperature for 1 hour before adding 2nM plasmid ...
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bioRxiv - Genomics 2023Quote: ... coli cultures using the EndoFree Plasmid Maxi Kit (Qiagen #12362). Low passage number (≤ passage 10 ...
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bioRxiv - Microbiology 2022Quote: ... 6XHis-tag fusion proteins were purified using Ni-NTA affinity chromatography (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and immunoblotted using a mouse monoclonal antibody against Strep-tag (Qiagen, 34850). Alkaline phosphatase conjugated to anti-mouse IgG (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding the recombinant RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). On Day 0 ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from strains marked with KI in Table S1 was extracted using DNeasy Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s guidelines for Gram-negative bacteria ...
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bioRxiv - Biophysics 2021Quote: The His-tagged TCF7L2 constructs were affinity purified using Ni-NTA agarose (Qiagen) and the tagged cleaved from the protein using thrombin ...
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bioRxiv - Microbiology 2019Quote: ... the fusion protein was purified using His-Bind columns (Qiagen, Venlo, The Netherlands) and analyzed by SDS-PAGE using gels containing 12% polyacrylamide ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged proteins were purified on Ni-NTA agarose (Qiagen; catalog no. 30761) and GST-tagged TopBP1 protein was purified on Glutathione Sepharose™ 4B (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Western blot analysis was performed using the Penta His HRP conjugate kit (Qiagen). To check for equal loading ...
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bioRxiv - Microbiology 2021Quote: ... His-N-WASP was first isolated using Ni-NTA beads (Qiagen, Valencia, CA) using a buffer with an imidazole gradient (20 mM Bis-Tris ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged TEV protease was removed by incubation with Ni-NTA (Qiagen) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... a penta-His HRP conjugate was used (1:4000, QIAGEN GmbH, Hilden, Germany). The detection of GFP was performed with the primary antibody anti-GFP (1:10000 ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant His-tagged GAPDH was purified using Ni-NTA Agarose (Qiagen, Valencia, USA) in native conditions according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: Assay mixes consisted of 25 nM ALEXA488-conjugated penta-His antibody (Qiagen # 35310), 50 μM DTT ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged fusion proteins were purified with a Ni-NTA column (Qiagen), and the elution fraction was incubated with TEV protease for 15-18 h at 4°C to cleave the N-terminal His-tag ...
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bioRxiv - Microbiology 2022Quote: ... His-Hcp was purified from the soluble fraction by NTA-resin chromatography (Qiagen). Purified protein was desalted with a PD-10 desalting column (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... Target protein was recovered and purified with a His-Trap affinity column (QIAGEN) according to manufacturers’ protocols ...
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bioRxiv - Cell Biology 2023Quote: ... Preparation of his-tagged recombinant proteins was performed according to manufacturer instructions (Qiagen). Preparation of GST-tagged recombinant proteins was performed according to manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen) and eluted with DPBS containing 150 mM imidazole.
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bioRxiv - Neuroscience 2024Quote: ... The His-tagged nanobodies were purified by using Ni-NTA purification column (Qiagen) followed by desalting step using disposable PD-10 desalting columns (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... His-tagged SLFN11 (residues 349-901) was purified by Ni-NTA beads (Qiagen). All purified GST- or His-tagged proteins were dialyzed against a buffer containing 50 mM Tris-HCl and 150 nM NaCl and validated by Coomassie blue-stained SDSLJPAGE.
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bioRxiv - Biochemistry 2019Quote: ... The cleaved tag and protease were removed by Ni-NTA affinity chromatography (Qiagen) and CLOCK PAS-AB was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... Mouse monoclonal antibody that recognizes the strep-tag epitope was purchased from Qiagen. Recombinant NifB expressed in E ...
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bioRxiv - Microbiology 2023Quote: The three cell mock communities (even, staggered, and spike-in) were diluted with Buffer AVE (Qiagen, Hilden, Germany) and split into eight replicates per mock and dilution for subsequent DNA extraction ...
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bioRxiv - Plant Biology 2020Quote: ... coli cells grown overnight at 30°C (Plasmid Mini Kit, QIAGEN), and its concentration measured by nanodrop ...
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bioRxiv - Neuroscience 2021Quote: ... coli DH5α and purified with a EndoFree Plasmid Maxi Kit (QIAGEN). The plasmids were then digested with AflIII and XbaI (both NEW ENGLAND BIOLABS ...
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bioRxiv - Cell Biology 2021Quote: ... coli and plasmid DNA was isolated by Plasmid Maxi-Prep (Qiagen). MCF10DCIS.com-GFP cells were cultured overnight to approximately 80% confluence ...
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bioRxiv - Molecular Biology 2021Quote: ... coli DH5α-pir and purified with QIAprep spin miniprep kit (Qiagen) according to the manufacturer’s recommendations.
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bioRxiv - Biochemistry 2022Quote: ... coli DH5α and purified using QIAprep spin mini-prep kit (Qiagen). All cDNA constructs were verified by DNA sequencing and services ...
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bioRxiv - Microbiology 2020Quote: ... coli strains was purified using the QIAprep Spin Miniprep Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... coli cells using the QIAprep spin miniprep kit (Qiagen, Hilden, Germany). Electrocompetent C ...