Labshake search
Citations for Qiagen :
451 - 500 of 2116 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... The His-tagged nanobodies were purified by using Ni-NTA purification column (Qiagen) followed by desalting step using disposable PD-10 desalting columns (Cytiva ...
-
bioRxiv - Biochemistry 2019Quote: ... The cleaved tag and protease were removed by Ni-NTA affinity chromatography (Qiagen) and CLOCK PAS-AB was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
-
bioRxiv - Microbiology 2023Quote: ... Mouse monoclonal antibody that recognizes the strep-tag epitope was purchased from Qiagen. Recombinant NifB expressed in E ...
-
bioRxiv - Microbiology 2019Quote: ... Influenza virus nucleic load was determined by qRT-PCR using influenza virus segment M specific primers (IAV-M; Table. 1) in 96-well plates according to the manufacturer instructions (OneStep RT-PCR, Qiagen, Toronto, Canada). Absolute quantification was performed using a standard curve based on a 10-fold serial dilution of a plasmid containing the A/Guinea Fowl/129/2015(H5N9 ...
-
bioRxiv - Microbiology 2023Quote: The three cell mock communities (even, staggered, and spike-in) were diluted with Buffer AVE (Qiagen, Hilden, Germany) and split into eight replicates per mock and dilution for subsequent DNA extraction ...
-
bioRxiv - Plant Biology 2020Quote: ... coli cells grown overnight at 30°C (Plasmid Mini Kit, QIAGEN), and its concentration measured by nanodrop ...
-
bioRxiv - Neuroscience 2021Quote: ... coli DH5α and purified with a EndoFree Plasmid Maxi Kit (QIAGEN). The plasmids were then digested with AflIII and XbaI (both NEW ENGLAND BIOLABS ...
-
bioRxiv - Cell Biology 2021Quote: ... coli and plasmid DNA was isolated by Plasmid Maxi-Prep (Qiagen). MCF10DCIS.com-GFP cells were cultured overnight to approximately 80% confluence ...
-
bioRxiv - Molecular Biology 2021Quote: ... coli DH5α-pir and purified with QIAprep spin miniprep kit (Qiagen) according to the manufacturer’s recommendations.
-
bioRxiv - Biochemistry 2022Quote: ... coli DH5α and purified using QIAprep spin mini-prep kit (Qiagen). All cDNA constructs were verified by DNA sequencing and services ...
-
bioRxiv - Microbiology 2020Quote: ... coli strains was purified using the QIAprep Spin Miniprep Kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... coli cells using the QIAprep spin miniprep kit (Qiagen, Hilden, Germany). Electrocompetent C ...
-
bioRxiv - Synthetic Biology 2020Quote: ... coli NEB5α and isolated by miniprep according to the manufacturers (Qiagen) protocol ...
-
bioRxiv - Genetics 2020Quote: ... coli cultures was performed with the QIAprep Spin Miniprep kit (Qiagen) and was combined with FastPrep (MP Biomedicals ...
-
bioRxiv - Genomics 2020Quote: ... coli culture following infection using the RNeasy Mini kit (Qiagen: #74106) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... coli (DH5α) and afterwards isolated with a mini-prep kit (Qiagen) according to the manufacturer’s instructions as described below ...
-
bioRxiv - Microbiology 2020Quote: ... coli M15 containing plasmid pREP4 to produce the LacI repressor (Qiagen). These strains were grown in 1L LB broth at 37°C with aeration until the OD 600 nm was 0.6-1.0 ...
-
bioRxiv - Plant Biology 2023Quote: ... coli cells using a miniprep kit from Qiagen (Germantown, MD, USA) following the recommended protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... coli DH5α and isolated using Qiagen QIAprep Spin Miniprep Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... coli C mutants was extracted using DNeasy Blood & Tissue kit (Qiagen). Quality of extracted DNA was confirmed before sequencing ...
-
bioRxiv - Biochemistry 2020Quote: ... 0.1 M Tris/HCl pH 8.5 (JCSG+ suite, Qiagen). Hanging drops of 1 ml protein at 2 mg/ml and 1 ml of precipitant (19% (w/v ...
-
bioRxiv - Microbiology 2021Quote: RNA and DNA was harvested from the BHK-SARS-2R_GFP_NeoR_NL cell line using the RNeasy mini kit (Qiagen) and the QIAamp genomic DNA mini kit (Qiagen) ...
-
bioRxiv - Cancer Biology 2019Quote: ... 200nM siRNA/well were used for transfection using 5µl/well of Hi-Perfect (Qiagen) following manufacturer’s recommendation.
-
bioRxiv - Biochemistry 2020Quote: ... but with an anti-His antibody (Qiagen, #34660 at a dilution of 1:5000) as primary ...
-
bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
-
bioRxiv - Biochemistry 2021Quote: ... Blots were probed with antibodies against His6 (penta-His Qiagen catalog #34460; 1:10,000), FLAG2 (Sigma catalog #A8592 ...
-
bioRxiv - Plant Biology 2022Quote: ... Blocking of the membranes was performed in anti His HRP conjugate blocking buffer (Qiagen). After blocking at room temperature for 2 h ...
-
bioRxiv - Microbiology 2022Quote: ... After incubation with the primary antibody from QIAGEN (Penta-His Antibody, 1:1000 dilution) at RT for 1 h under shaking ...
-
bioRxiv - Genomics 2022Quote: ... 25 fmol of the exogenous synthetic spike-in control Caenorhabditis elegans miRNA cel-miR-39 (Qiagen, Cat. No. 219610) was spiked into samples at the beginning of the extraction procedure to check both the extraction of miRNAs and the efficiency of the cDNA synthesis ...
-
bioRxiv - Cancer Biology 2022Quote: ... after adding 1.0×10^6 copies/µL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... coli BL21(DE3)pLysS and purified through Ni-NTA-agarose (Qiagen, #30210). Proteins were eluted by 200 mM imidazole ...
-
bioRxiv - Cancer Biology 2019Quote: ... coli BL21(DE3)pLysS and purified through Ni-NTA-agarose (Qiagen, #30210) as previously described (21) ...
-
bioRxiv - Biophysics 2020Quote: ... coli BL21 (DE3) (38) and purified using Ni-NTA affinity column (Qiagen), HiTrapTM Heparin HP column and HiTrapTM Q HP column (GE Healthcare ...
-
bioRxiv - Plant Biology 2021Quote: ... coli strain BL21(BE3) and purified using Ni-NTA agarose chromatography (Qiagen) as previously described (Xu et al. ...
-
bioRxiv - Biochemistry 2021Quote: ... coli cells and purified by FPLC using a Ni-NTA column (Qiagen) and Heparin (GE Healthcare ...
-
bioRxiv - Cell Biology 2021Quote: ... coli and purified by Nickel affinity chromatography (Ni2+-NTA-sepharose resin, Qiagen). His6-FUBI-eS30 WT and AA constructs were expressed in E ...
-
bioRxiv - Bioengineering 2022Quote: ... coli and purified using a QiaPrep Spin Miniprep kit (Qiagen, Venlo, NL). All plasmids were subjected to Sanger sequencing to verify the presence of the correct sing guide RNA (sgRNA ...
-
bioRxiv - Genomics 2023Quote: ... coli RNA was isolated using an RNEasy column (Qiagen, Germantown, MD, USA) followed by polyadenylation with an E ...
-
bioRxiv - Microbiology 2023Quote: ... coli was extracted with the MagAttract HMW DNA kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol for DNA extraction from Gram-negative bacteria ...
-
bioRxiv - Biochemistry 2023Quote: ... coli using CLC Genomic Workbench v9.01 (QIAGEN® CLC bio, Aarhus, Denmark). Furthermore ...
-
bioRxiv - Cell Biology 2023Quote: ... coli bacteria (XL1Blue strain or DH5α strain) using plasmid extraction kits (QIAGEN; Venlo ...
-
bioRxiv - Microbiology 2024Quote: ... Plasmids were prepared from VectorBuilder Escherichia coli stocks using Plasmid Miniprep (Qiagen) and ampicillin selection ...
-
bioRxiv - Biochemistry 2021Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
-
bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
-
bioRxiv - Microbiology 2020Quote: ... were coated with 100 ng/well of mouse anti-Penta His BSA-free antibody (Qiagen) in PBS ...
-
bioRxiv - Biochemistry 2022Quote: ... a 1:2000 dilution of a mouse anti-Penta-His Alexa Fluor 647 conjugate (Qiagen) was used.
-
bioRxiv - Biochemistry 2021Quote: ... Monoclonal mouse anti-StrepII and anti-His antibodies were obtained from QIAGEN (catalog number 34850) and Genscript (catalog number A00186) ...
-
bioRxiv - Microbiology 2022Quote: ... BapR-CPD-His was affinity purified from cleared lysates using Ni-NTA agarose beads (Qiagen) with gentle rocking at 4°C for 2 hours ...
-
bioRxiv - Immunology 2022Quote: ... after which the cells were stained with anti-Penta-His-AF647 (1 µg/mL; Qiagen) for 30 minutes at 4°C ...