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Citations for Qiagen :
151 - 200 of 848 citations for Recombinant Mouse F2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... His-Penta-Conjugate (Qiagen) was then used as the secondary antibody (1:5000) ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bands were transferred to a nitrocellulose membrane and probed with primary antibodies [mouse anti-His (Qiagen) to detect CAT ...
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bioRxiv - Immunology 2020Quote: ... and detection was performed using anti-His antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse antibody labeled with AlexaFluor647 (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... DNA of F2s was extracted from silica dried young leaves using Qiagen DNEasy reagents and Magattract beads (Qiagen, Inc.), libraries were prepared following the protocol of RipTide High Throughput Rapid DNA Library Preparation kit (iGenomX ...
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bioRxiv - Genetics 2020Quote: We extracted gDNA from F0 and F1 worms and from F2 cercariae using the Blood and Tissue kit (Qiagen), following the manufacturer protocol ...
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bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
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bioRxiv - Cell Biology 2022Quote: ... His-EHD1 was purified by binding with Ni2+-NTA His-bind slurry (Qiagen, Germany) and eluting with imidazole as described previously (65) ...
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bioRxiv - Biophysics 2021Quote: ... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
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bioRxiv - Cell Biology 2021Quote: ... and detection was performed using anti-His primary antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse secondary antibody labeled with AlexaFluor647 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... and detection was performed using anti-His primary antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse secondary antibody labeled with AlexaFluor647 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... and anti-His (Qiagen 34660) antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... anti-RGS-His (Qiagen, 34610), anti-Vinculin (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-penta-His (34660, Qiagen), anti-GFP-HRP (B-2 ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His (Qiagen 34660) antibodies ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from the red blood cells of the parents and 99 F2 hybrid females using the DNeasy Blood & Tissue Kit (Qiagen). The library was constructed according to a previously described method [27] ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from all F2 individuals (n = 118) and their parental lines using the DNeasy Plant Mini Kit (Qiagen). The obtained DNA samples were digested with PstI and MspI to construct a double digest restriction-site associated DNA sequencing (ddRAD-Seq ...
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bioRxiv - Genetics 2022Quote: High molecular weight (HMW) genomic DNA from 538 F2 spleen samples was isolated via the MagAttract HMW DNA Kit (Qiagen) at the University of Michigan and transferred to the University of California San Diego for further processing ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was extracted from young leaf tissue collected from each F0 parent (flash frozen) and each F2 (desiccated using silica gel) using Qiagen DNEasy reagents and Magattract beads (Qiagen, Inc.). Sequencing libraries for the two F0 parents were prepared using the NEBNext Ultra II kit (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was extracted from 37 F2 plants that did not show a HR using a DNeasy Plant Mini Kit (Qiagen, Germany). This DNA was pooled and sequenced by DNA Link (Republic of Korea ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was isolated from tails of F2 progeny between WSB and B6 mice using DNeasy Tissue kit (Qiagen, Hilden, Germany). Genome-wide SNP genotyping was performed with a new Mouse Universal Genotyping Array (MiniMUGA) ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was isolated from individuals of parental lines and F2 populations with a DNeasy Plant Kit 96 (Qiagen, Hilden, Germany). Each DNA sample was diluted to a final concentration of 25 ng/μL ...
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bioRxiv - Microbiology 2019Quote: ... and HRP-penta-His antibody (Qiagen) and HRP-anti-mouse IgG (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... HIS-tag (Qiagen 34610 1:100) and HER3 (R&D Systems AF4518 1:400) ...
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bioRxiv - Microbiology 2019Quote: ... Penta His HRP conjugate (Qiagen, 34460) was diluted 1:5000 in 1% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Pulled-down fractions were analysed by SDS-PAGE or Western blot using rabbit antibodies against KtrB or mouse against His-tag (Qiagen).
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bioRxiv - Developmental Biology 2021Quote: ... About 100 sterile F2 worms were identified and pooled for genomic DNA prep by standard column purification (Qiagen Minelute PCR purification Kits).
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bioRxiv - Genetics 2019Quote: Genomic DNA was isolated from tails of F2 intercross between CAST/EiJ (CAST) and WSB/EiJ (WSB) mice using DNeasy Tissue kit (Qiagen, Hilden, Germany). Genome-wide SNP genotyping was performed with a Mouse Universal Genotyping Array (MUGA ...
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bioRxiv - Genetics 2019Quote: Genomic DNA was isolated from Lerik1-3 x ICE106/ICE107 F2 and F3 individuals and from ICE79 x Don-0 F2 individuals using a Biosprint 96 instrument and the BioSprint 96 DNA Plant Kit (Qiagen, Hilden, Germany).The individuals represented all classes of segregating phenotypes ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was isolated from young leaves of the parental lines and F1 and F2 populations using the DNeasy Plant Kit 96 (Qiagen, Hilden, Germany). CmACS-7 and CmWIP1 were genotyped according to Boualem et al ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from 833 high and low weight intercross F2 offspring and all F1 and F0 parents with a DNeasy (Qiagen, Hilden, Germany) extraction kit using the modified mouse tail protocol of Fritz et al ...
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bioRxiv - Cell Biology 2022Quote: ... Drosophila S2 cells were transfected with mNeonGreen-tagged kinesin-1 full-length heavy chain (KHC) and Halo-tagged PLP584-1811 (Effectene, QIAGEN). The lysates were harvested for TIRF motility assays at ∼48 h post-transfection ...
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bioRxiv - Synthetic Biology 2020Quote: ... For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen, #34660) and then incubated with 1:10000 peroxidase labelled anti-mouse IgG secondary antibody (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... The recombinant plasmids for mouse vaccination were prepared using an EndoFree Plasmid Purification Kit (Cat# 12391, Qiagen, Hilden, USA).
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bioRxiv - Biochemistry 2023Quote: ... Cleaved CK1δ ΔC was further purified away from His-GST and His-TEV using Ni-NTA resin (Qiagen) and subsequent size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... the Penta-His HRP Conjugate Kit (Qiagen) was used following manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... anti-RGS-His (Qiagen, 1:2000, 34610), anti-Myc (ChromoTek ...
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bioRxiv - Cell Biology 2022Quote: ... The Penta-His HRP Conjugate Kit (Qiagen) was used for detection of His-tagged proteins according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was extracted from plant cells and Hi-C libraries were prepared using EpiTect Hi-C Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... Genomic DNA was isolated from the head and thorax of each F2 offspring and the parents and grandparents using the Qiagen DNeasy kit (Qiagen, Inc., Valencia, CA, USA) with an RNase A treatment following an overnight tissue lysis incubation at 55°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing His-eIF4A1 or His-eIF4A2 was incubated with a 1.5 ml bed volume of Ni-NTA Superflow (Qiagen, 30430) or a 0.75 ml bed volume of Ni-NTA agarose (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... coli strains expressing (His)6-Clr4 and (His)6-Swi6 and allowed to bind at 4°C overnight to Ni-NTA resin (Qiagen; binding capacity 5-10mg protein/ml resin) ...
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bioRxiv - Molecular Biology 2022Quote: Drosophila S2 cells were transiently transfected with pAc-brm-FLAG-6xHIS in combination with either pAc-clk-V5-HIS or pAc-V5-HIS empty plasmid using Effectene (Qiagen). For cycloheximide experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transiently transfected with pAc-brm-FLAG-6xHIS in combination with pAc-clk-V5-HIS or pAc-V5-HIS empty plasmid using Effectene (Qiagen). Cells were harvested 48 hours after transfection for processing ...
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bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... Penta-His Antibody (Qiagen, 34660, IB: 1:1000) and Y188 to APP C-terminus (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with anti-penta-his biotin antibody (Qiagen) for 1 and half hours at 4°C ...
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bioRxiv - Immunology 2019Quote: ... were inserted Bam HI site of pET30a (Qiagen) to produce pET30a-YFP ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies included: anti-Penta-His (QIAGEN, #34650), anti-FLAG M2 (Sigma-Aldrich ...